Reviewed, UniProtKB/Swiss-Prot Q11126 (FUT3_BOVIN)

Names and origin

Protein names

Recommended name:
    Galactoside 3(4)-L-fucosyltransferase
    EC=2.4.1.65
Alternative name(s):
    Blood group Lewis alpha-4-fucosyltransferase
      Short name=Lewis FT
    Fucosyltransferase 3
    Fucosyltransferase III
      Short name=FucT-III
    FUTB

Gene names

Name:

FUT3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of sialyl Lewis X and Lewis X/SSEA-1 antigens. It may be involved in blood group Lewis determination.
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Tissue specificity	Liver, kidney, lung and brain.
Miscellaneous	Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Galactoside 3(4)-L-fucosyltransferase		PRO_0000221095
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 34	20	Signal-anchor for type II membrane protein Potential
	Topological domain	35 – 365	331	Lumenal Potential
Amino acid modifications
	Glycosylation	100	1	N-linked (GlcNAc...) Probable
	Glycosylation	158	1	N-linked (GlcNAc...) Probable
	Glycosylation	189	1	N-linked (GlcNAc...) Probable

Sequences

	Sequence		Length	Mass (Da)
	Q11126-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 18715A361B0025D3	FASTA	365	42,654
	10 20 30 40 50 60 MYPPGCAKVK CSWHHCLPGL LLQLLLALCF FSYLRMSQEK PKPKPMWVSE LGAPSQATEG 70 80 90 100 110 120 SSAHLPLRVL LWTWPFNQPV ALSRCSELWP GTADCQLTVN RSEYPQADAV LVHHREVSHR 130 140 150 160 170 180 PQMQLPPSPR PPGQRWVWFS MESPSNCLKL KDLDGYFNLT MSYRRDSDIF MPYGWLEPWP 190 200 210 220 230 240 SQPVETLLNI SAKTKLVAWV VSNWNTDSIR VQYYKLLKPH LQVDVYGRFH TPLPHALMAK 250 260 270 280 290 300 QLSQYKFYLA FENSLHPDYI TEKLWKNALQ AWAVPVVLGP SRVNYEQFLP PKAFIHVEDF 310 320 330 340 350 360 QSPKDLAQYL LALDKDYASY LNYFRWRETL RPRSFSWALM FCKACWKLQQ EPRYQTVPSI ASWFQ

References

[1]	"Molecular cloning and expression of a bovine alpha(1,3)-fucosyltransferase gene homologous to a putative ancestor gene of the human FUT3-FUT5-FUT6 cluster." Oulmouden A., Wierinckx A., Petit J.-M., Costache M., Palcic M.M., Mollicone R., Oriol R., Julien R. J. Biol. Chem. 272:8764-8773(1997) [PubMed: 9079712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic organization of futb encoding a bovine alpha 3-fucosyltransferase: exons in human orthologous genes emerged from ancestral intronic sequences." Wierinckx A., Mercier D., Oulmouden A., Petit J.-M., Julien R. Mol. Biol. Evol. 16:1535-1547(1999) [PubMed: 10555285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X87810 Genomic DNA. Translation: CAA61079.1. AJ132772 Genomic DNA. Translation: CAA10771.1.
IPI	IPI00685349.
UniGene	Bt.4074
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
Genome annotation databases
Ensembl	ENSBTAG00000000414. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	Q11126.
Enzyme and pathway databases
BRENDA	2.4.1.65. 251.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT3_BOVIN

Accession

Primary (citable) accession number: Q11126
Secondary accession number(s): Q4LDG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)