Q11068 (MGAT1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
EC=2.4.1.101

Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name=GNT-I
Short name=GlcNAc-T I

Gene names

Name:	gly-13
ORF Names:	B0416.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Manganese. The cofactor is mostly bound to the substrate By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Developmental stage	Expressed throughout development. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase		PRO_0000191388
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 23	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 449	426	Lumenal Potential
Sites
	Active site	279	1	Proton acceptor Potential
	Metal binding	201	1	Manganese By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	130	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity
	Binding site	312	1	Substrate By similarity
Amino acid modifications
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Disulfide bond	100 ↔ 131		By similarity
	Disulfide bond	227 ↔ 293		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11068 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 8448210B2A78FC88	FASTA	449	52,151
	10 20 30 40 50 60 MHAVTKIFII FIFVFILWTL YVENDITNRT RNTDNIDDLL ESANRLERLL KFEAKKIAAL 70 80 90 100 110 120 AEDVHKIRAN RKGKHVIMEE MVSQDLKQWK DPIPVLVFSC NRAMAVRDHV EKLIRYRPSQ 130 140 150 160 170 180 EKFPIIVTQD CDNENVKNEV KKFGDKVEYI KHLAGDKANI TIPPSHRQYT AYYRIARHYK 190 200 210 220 230 240 LALNHVFVDK GYSSVIITED DLDISPDFFS YFSSTRYLLE NDEKLWCVTA WNDNGKQENI 250 260 270 280 290 300 DMTAASTLYR SDFFAGLGWM MSSKTWHELE PIWPVGFWDD WMRDPARRKD RQCIRPEISR 310 320 330 340 350 360 TGMMSYGKEG ASKGQFFSKH LAKIKVNDKY INFGKIDLDY LLPANFAKKT NLEVMKEAVE 370 380 390 400 410 420 LSIDNVASFV LSSENKGKSV RVMYDGNIDY IRKADKLHIM HDFKAGVPRT AYDGIVTCFI 430 440 NGIRIYLVPD RTKVSAYNPD WSVPPSFGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development." Chen S., Zhou S., Sarkar M., Spence A.M., Schachter H. J. Biol. Chem. 274:288-297(1999) [PubMed: 9867843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082010 mRNA. Translation: AAD03022.1. FO080200 Genomic DNA. Translation: CCD61925.1.
PIR	T43340.
RefSeq	NP_509566.1. NM_077165.4.
UniGene	Cel.19612.
3D structure databases
ProteinModelPortal	Q11068.
ModBase	Search...
Protein-protein interaction databases
STRING	Q11068.
Protein family/group databases
CAZy	GT13. Glycosyltransferase Family 13.
Proteomic databases
PRIDE	Q11068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0416.6; B0416.6; B0416.6.
GeneID	181160.
KEGG	cel:B0416.6.
UCSC	B0416.6. c. elegans.
Organism-specific databases
CTD	181160.
WormBase	B0416.6; CE26434; WBGene00001638; gly-13.
Phylogenomic databases
eggNOG	meNOG05989.
GeneTree	EMGT00050000010523.
HOGENOM	HBG717617.
InParanoid	Q11068.
OMA	PPDHRKF.
PhylomeDB	Q11068.
Gene expression databases
ArrayExpress	Q11068.
Family and domain databases
InterPro	IPR004139. Glyco_trans_13. [Graphical view]
KO	K00726.
PANTHER	PTHR10468. Glyco_trans_13. 1 hit.
Pfam	PF03071. GNT-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	912664.

Entry information

Entry name

MGAT1_CAEEL

Accession

Primary (citable) accession number: Q11068
Secondary accession number(s): O76776

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 14, 2001
Last modified:	December 14, 2011
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project