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Q11068

- MGAT1_CAEEL

UniProt

Q11068 - MGAT1_CAEEL

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Protein

Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

gly-13

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Manganese. The cofactor is mostly bound to the substrate (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei130 – 1301SubstrateBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei200 – 2001SubstrateBy similarity
Metal bindingi201 – 2011ManganeseBy similarity
Active sitei279 – 2791Proton acceptorSequence Analysis
Binding sitei312 – 3121SubstrateBy similarity

GO - Molecular functioni

  1. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: WormBase
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein N-linked glycosylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_183152. N-glycan trimming and elongation in the cis-Golgi.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT13. Glycosyltransferase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:gly-13
ORF Names:B0416.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiB0416.6; CE26434; WBGene00001638; gly-13.

Subcellular locationi

GO - Cellular componenti

  1. Golgi medial cisterna Source: WormBase
  2. integral component of membrane Source: UniProtKB-KW
  3. perinuclear region of cytoplasm Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000191388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi100 ↔ 131By similarity
Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi227 ↔ 293By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ11068.
PRIDEiQ11068.

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ11068.
SMRiQ11068. Positions 93-442.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Topological domaini24 – 449426LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2317Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148227.
GeneTreeiENSGT00530000063632.
HOGENOMiHOG000247055.
InParanoidiQ11068.
KOiK00726.
OMAiHEETAQV.
OrthoDBiEOG7R56SN.
PhylomeDBiQ11068.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10468. PTHR10468. 1 hit.
PfamiPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q11068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHAVTKIFII FIFVFILWTL YVENDITNRT RNTDNIDDLL ESANRLERLL
60 70 80 90 100
KFEAKKIAAL AEDVHKIRAN RKGKHVIMEE MVSQDLKQWK DPIPVLVFSC
110 120 130 140 150
NRAMAVRDHV EKLIRYRPSQ EKFPIIVTQD CDNENVKNEV KKFGDKVEYI
160 170 180 190 200
KHLAGDKANI TIPPSHRQYT AYYRIARHYK LALNHVFVDK GYSSVIITED
210 220 230 240 250
DLDISPDFFS YFSSTRYLLE NDEKLWCVTA WNDNGKQENI DMTAASTLYR
260 270 280 290 300
SDFFAGLGWM MSSKTWHELE PIWPVGFWDD WMRDPARRKD RQCIRPEISR
310 320 330 340 350
TGMMSYGKEG ASKGQFFSKH LAKIKVNDKY INFGKIDLDY LLPANFAKKT
360 370 380 390 400
NLEVMKEAVE LSIDNVASFV LSSENKGKSV RVMYDGNIDY IRKADKLHIM
410 420 430 440
HDFKAGVPRT AYDGIVTCFI NGIRIYLVPD RTKVSAYNPD WSVPPSFGE
Length:449
Mass (Da):52,151
Last modified:August 14, 2001 - v2
Checksum:i8448210B2A78FC88
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082010 mRNA. Translation: AAD03022.1.
FO080200 Genomic DNA. Translation: CCD61925.1.
PIRiT43340.
RefSeqiNP_509566.1. NM_077165.5.
UniGeneiCel.19612.

Genome annotation databases

EnsemblMetazoaiB0416.6; B0416.6; WBGene00001638.
GeneIDi181160.
KEGGicel:CELE_B0416.6.
UCSCiB0416.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082010 mRNA. Translation: AAD03022.1 .
FO080200 Genomic DNA. Translation: CCD61925.1 .
PIRi T43340.
RefSeqi NP_509566.1. NM_077165.5.
UniGenei Cel.19612.

3D structure databases

ProteinModelPortali Q11068.
SMRi Q11068. Positions 93-442.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT13. Glycosyltransferase Family 13.

Proteomic databases

PaxDbi Q11068.
PRIDEi Q11068.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai B0416.6 ; B0416.6 ; WBGene00001638 .
GeneIDi 181160.
KEGGi cel:CELE_B0416.6.
UCSCi B0416.6. c. elegans.

Organism-specific databases

CTDi 181160.
WormBasei B0416.6 ; CE26434 ; WBGene00001638 ; gly-13.

Phylogenomic databases

eggNOGi NOG148227.
GeneTreei ENSGT00530000063632.
HOGENOMi HOG000247055.
InParanoidi Q11068.
KOi K00726.
OMAi HEETAQV.
OrthoDBi EOG7R56SN.
PhylomeDBi Q11068.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_183152. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

NextBioi 912664.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10468. PTHR10468. 1 hit.
Pfami PF03071. GNT-I. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development."
    Chen S., Zhou S., Sarkar M., Spence A.M., Schachter H.
    J. Biol. Chem. 274:288-297(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiMGAT1_CAEEL
AccessioniPrimary (citable) accession number: Q11068
Secondary accession number(s): O76776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 14, 2001
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3