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Q11068

- MGAT1_CAEEL

UniProt

Q11068 - MGAT1_CAEEL

Protein

Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

gly-13

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans By similarity.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

    Cofactori

    Manganese. The cofactor is mostly bound to the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei102 – 1021SubstrateBy similarity
    Binding sitei130 – 1301SubstrateBy similarity
    Binding sitei178 – 1781SubstrateBy similarity
    Binding sitei200 – 2001SubstrateBy similarity
    Metal bindingi201 – 2011ManganeseBy similarity
    Active sitei279 – 2791Proton acceptorSequence Analysis
    Binding sitei312 – 3121SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: WormBase
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein N-linked glycosylation Source: WormBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_183152. N-glycan trimming and elongation in the cis-Golgi.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT13. Glycosyltransferase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
    Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
    Short name:
    GNT-I
    Short name:
    GlcNAc-T I
    Gene namesi
    Name:gly-13
    ORF Names:B0416.6
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiB0416.6; CE26434; WBGene00001638; gly-13.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi medial cisterna Source: WormBase
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. perinuclear region of cytoplasm Source: WormBase

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000191388Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi100 ↔ 131By similarity
    Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi227 ↔ 293By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ11068.
    PRIDEiQ11068.

    Expressioni

    Developmental stagei

    Expressed throughout development.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ11068.
    SMRiQ11068. Positions 93-442.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini24 – 449426LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2317Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 13 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148227.
    GeneTreeiENSGT00530000063632.
    HOGENOMiHOG000247055.
    InParanoidiQ11068.
    KOiK00726.
    OMAiHEETAQV.
    OrthoDBiEOG7R56SN.
    PhylomeDBiQ11068.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10468. PTHR10468. 1 hit.
    PfamiPF03071. GNT-I. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q11068-1 [UniParc]FASTAAdd to Basket

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    MHAVTKIFII FIFVFILWTL YVENDITNRT RNTDNIDDLL ESANRLERLL    50
    KFEAKKIAAL AEDVHKIRAN RKGKHVIMEE MVSQDLKQWK DPIPVLVFSC 100
    NRAMAVRDHV EKLIRYRPSQ EKFPIIVTQD CDNENVKNEV KKFGDKVEYI 150
    KHLAGDKANI TIPPSHRQYT AYYRIARHYK LALNHVFVDK GYSSVIITED 200
    DLDISPDFFS YFSSTRYLLE NDEKLWCVTA WNDNGKQENI DMTAASTLYR 250
    SDFFAGLGWM MSSKTWHELE PIWPVGFWDD WMRDPARRKD RQCIRPEISR 300
    TGMMSYGKEG ASKGQFFSKH LAKIKVNDKY INFGKIDLDY LLPANFAKKT 350
    NLEVMKEAVE LSIDNVASFV LSSENKGKSV RVMYDGNIDY IRKADKLHIM 400
    HDFKAGVPRT AYDGIVTCFI NGIRIYLVPD RTKVSAYNPD WSVPPSFGE 449
    Length:449
    Mass (Da):52,151
    Last modified:August 14, 2001 - v2
    Checksum:i8448210B2A78FC88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF082010 mRNA. Translation: AAD03022.1.
    FO080200 Genomic DNA. Translation: CCD61925.1.
    PIRiT43340.
    RefSeqiNP_509566.1. NM_077165.5.
    UniGeneiCel.19612.

    Genome annotation databases

    EnsemblMetazoaiB0416.6; B0416.6; WBGene00001638.
    GeneIDi181160.
    KEGGicel:CELE_B0416.6.
    UCSCiB0416.6. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF082010 mRNA. Translation: AAD03022.1 .
    FO080200 Genomic DNA. Translation: CCD61925.1 .
    PIRi T43340.
    RefSeqi NP_509566.1. NM_077165.5.
    UniGenei Cel.19612.

    3D structure databases

    ProteinModelPortali Q11068.
    SMRi Q11068. Positions 93-442.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT13. Glycosyltransferase Family 13.

    Proteomic databases

    PaxDbi Q11068.
    PRIDEi Q11068.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai B0416.6 ; B0416.6 ; WBGene00001638 .
    GeneIDi 181160.
    KEGGi cel:CELE_B0416.6.
    UCSCi B0416.6. c. elegans.

    Organism-specific databases

    CTDi 181160.
    WormBasei B0416.6 ; CE26434 ; WBGene00001638 ; gly-13.

    Phylogenomic databases

    eggNOGi NOG148227.
    GeneTreei ENSGT00530000063632.
    HOGENOMi HOG000247055.
    InParanoidi Q11068.
    KOi K00726.
    OMAi HEETAQV.
    OrthoDBi EOG7R56SN.
    PhylomeDBi Q11068.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_183152. N-glycan trimming and elongation in the cis-Golgi.

    Miscellaneous databases

    NextBioi 912664.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10468. PTHR10468. 1 hit.
    Pfami PF03071. GNT-I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development."
      Chen S., Zhou S., Sarkar M., Spence A.M., Schachter H.
      J. Biol. Chem. 274:288-297(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiMGAT1_CAEEL
    AccessioniPrimary (citable) accession number: Q11068
    Secondary accession number(s): O76776
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3