Reviewed, UniProtKB/Swiss-Prot Q11068 (MGAT1_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
    EC=2.4.1.101
Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
      Short name=GlcNAc-T I
      Short name=GNT-I

Gene names

Name:	gly-13
ORF Names:	B0416.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Developmental stage	Expressed throughout development. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase		PRO_0000191388
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 23	17	Signal-anchor for type II membrane protein Potential
	Topological domain	24 – 449	426	Lumenal Potential
Amino acid modifications
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q11068-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 8448210B2A78FC88	FASTA	449	52,151
	10 20 30 40 50 60 MHAVTKIFII FIFVFILWTL YVENDITNRT RNTDNIDDLL ESANRLERLL KFEAKKIAAL 70 80 90 100 110 120 AEDVHKIRAN RKGKHVIMEE MVSQDLKQWK DPIPVLVFSC NRAMAVRDHV EKLIRYRPSQ 130 140 150 160 170 180 EKFPIIVTQD CDNENVKNEV KKFGDKVEYI KHLAGDKANI TIPPSHRQYT AYYRIARHYK 190 200 210 220 230 240 LALNHVFVDK GYSSVIITED DLDISPDFFS YFSSTRYLLE NDEKLWCVTA WNDNGKQENI 250 260 270 280 290 300 DMTAASTLYR SDFFAGLGWM MSSKTWHELE PIWPVGFWDD WMRDPARRKD RQCIRPEISR 310 320 330 340 350 360 TGMMSYGKEG ASKGQFFSKH LAKIKVNDKY INFGKIDLDY LLPANFAKKT NLEVMKEAVE 370 380 390 400 410 420 LSIDNVASFV LSSENKGKSV RVMYDGNIDY IRKADKLHIM HDFKAGVPRT AYDGIVTCFI 430 440 NGIRIYLVPD RTKVSAYNPD WSVPPSFGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development." Chen S., Zhou S., Sarkar M., Spence A.M., Schachter H. J. Biol. Chem. 274:288-297(1999) [PubMed: 9867843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF082010 mRNA. Translation: AAD03022.1. U23516 Genomic DNA. Translation: AAG38885.1.
PIR	T43340.
RefSeq	NP_509566.1.
UniGene	Cel.19612
3D structure databases
HSSP	HSSP built from PDB template 1FO8 based on UniProtKB P27115.
ModBase	Search...
Protein family/group databases
CAZy	GT13. Glycosyltransferase Family 13.
Genome annotation databases
Ensembl	B0416.6. Caenorhabditis elegans. [Contig view]
GeneID	181160.
KEGG	cel:B0416.6.
Organism-specific databases
WormBase	WBGene00001638. gly-13.
WormPep	B0416.6. CE26434. [WorfDB]
Phylogenomic databases
OMA	Q11068. IACNRVT.
Enzyme and pathway databases
BRENDA	2.4.1.101. 672.
Gene expression databases
ArrayExpress	Q11068.
Family and domain databases
InterPro	IPR004139. Glyco_trans_13. [Graphical view]
PANTHER	PTHR10468. Glyco_trans_13. 1 hit.
Pfam	PF03071. GNT-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	912664.

Entry information

Entry name

MGAT1_CAEEL

Accession

Primary (citable) accession number: Q11068
Secondary accession number(s): O76776

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 14, 2001
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project