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Q11068 (MGAT1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

EC=2.4.1.101
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name=GNT-I
Short name=GlcNAc-T I
Gene names
Name:gly-13
ORF Names:B0416.6
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactor

Manganese. The cofactor is mostly bound to the substrate By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Developmental stage

Expressed throughout development. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
PRO_0000191388

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2317Helical; Signal-anchor for type II membrane protein; Potential
Topological domain24 – 449426Lumenal Potential

Sites

Active site2791Proton acceptor Potential
Metal binding2011Manganese By similarity
Binding site1021Substrate By similarity
Binding site1301Substrate By similarity
Binding site1781Substrate By similarity
Binding site2001Substrate By similarity
Binding site3121Substrate By similarity

Amino acid modifications

Glycosylation281N-linked (GlcNAc...) Potential
Glycosylation1591N-linked (GlcNAc...) Potential
Disulfide bond100 ↔ 131 By similarity
Disulfide bond227 ↔ 293 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q11068 [UniParc].

Last modified August 14, 2001. Version 2.
Checksum: 8448210B2A78FC88

FASTA44952,151
        10         20         30         40         50         60 
MHAVTKIFII FIFVFILWTL YVENDITNRT RNTDNIDDLL ESANRLERLL KFEAKKIAAL 

        70         80         90        100        110        120 
AEDVHKIRAN RKGKHVIMEE MVSQDLKQWK DPIPVLVFSC NRAMAVRDHV EKLIRYRPSQ 

       130        140        150        160        170        180 
EKFPIIVTQD CDNENVKNEV KKFGDKVEYI KHLAGDKANI TIPPSHRQYT AYYRIARHYK 

       190        200        210        220        230        240 
LALNHVFVDK GYSSVIITED DLDISPDFFS YFSSTRYLLE NDEKLWCVTA WNDNGKQENI 

       250        260        270        280        290        300 
DMTAASTLYR SDFFAGLGWM MSSKTWHELE PIWPVGFWDD WMRDPARRKD RQCIRPEISR 

       310        320        330        340        350        360 
TGMMSYGKEG ASKGQFFSKH LAKIKVNDKY INFGKIDLDY LLPANFAKKT NLEVMKEAVE 

       370        380        390        400        410        420 
LSIDNVASFV LSSENKGKSV RVMYDGNIDY IRKADKLHIM HDFKAGVPRT AYDGIVTCFI 

       430        440 
NGIRIYLVPD RTKVSAYNPD WSVPPSFGE 

« Hide

References

« Hide 'large scale' references
[1]"Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development."
Chen S., Zhou S., Sarkar M., Spence A.M., Schachter H.
J. Biol. Chem. 274:288-297(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF082010 mRNA. Translation: AAD03022.1.
FO080200 Genomic DNA. Translation: CCD61925.1.
PIRT43340.
RefSeqNP_509566.1. NM_077165.5.
UniGeneCel.19612.

3D structure databases

ProteinModelPortalQ11068.
SMRQ11068. Positions 93-442.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT13. Glycosyltransferase Family 13.

Proteomic databases

PaxDbQ11068.
PRIDEQ11068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaB0416.6; B0416.6; B0416.6.
GeneID181160.
KEGGcel:CELE_B0416.6.
UCSCB0416.6. c. elegans.

Organism-specific databases

CTD181160.
WormBaseB0416.6; CE26434; WBGene00001638; gly-13.

Phylogenomic databases

eggNOGNOG148227.
HOGENOMHOG000247055.
InParanoidQ11068.
KOK00726.
OMASFWDDWM.
OrthoDBEOG7R56SN.
PhylomeDBQ11068.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR004139. Glyco_trans_13.
[Graphical view]
PANTHERPTHR10468. PTHR10468. 1 hit.
PfamPF03071. GNT-I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio912664.

Entry information

Entry nameMGAT1_CAEEL
AccessionPrimary (citable) accession number: Q11068
Secondary accession number(s): O76776
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 14, 2001
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase