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Protein

Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

gly-13

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein].

Cofactori

Mn2+By similarityNote: The cofactor is mostly bound to the substrate.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei178SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Metal bindingi201ManganeseBy similarity1
Active sitei279Proton acceptorSequence analysis1
Binding sitei312SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation Source: WormBase

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-964739 N-glycan trimming and elongation in the cis-Golgi
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:gly-13
ORF Names:B0416.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiB0416.6 ; CE26434 ; WBGene00001638 ; gly-13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini24 – 449LumenalSequence analysisAdd BLAST426

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913881 – 449Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdd BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi100 ↔ 131By similarity
Glycosylationi159N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi227 ↔ 293By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ11068
PaxDbiQ11068
PeptideAtlasiQ11068

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

BgeeiWBGene00001638

Interactioni

Protein-protein interaction databases

STRINGi6239.B0416.6

Structurei

3D structure databases

ProteinModelPortaliQ11068
SMRiQ11068
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1413 Eukaryota
ENOG410XQD8 LUCA
GeneTreeiENSGT00530000063632
HOGENOMiHOG000247055
InParanoidiQ11068
KOiK00726
OMAiQDPSLWC
OrthoDBiEOG091G0A29
PhylomeDBiQ11068

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q11068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHAVTKIFII FIFVFILWTL YVENDITNRT RNTDNIDDLL ESANRLERLL
60 70 80 90 100
KFEAKKIAAL AEDVHKIRAN RKGKHVIMEE MVSQDLKQWK DPIPVLVFSC
110 120 130 140 150
NRAMAVRDHV EKLIRYRPSQ EKFPIIVTQD CDNENVKNEV KKFGDKVEYI
160 170 180 190 200
KHLAGDKANI TIPPSHRQYT AYYRIARHYK LALNHVFVDK GYSSVIITED
210 220 230 240 250
DLDISPDFFS YFSSTRYLLE NDEKLWCVTA WNDNGKQENI DMTAASTLYR
260 270 280 290 300
SDFFAGLGWM MSSKTWHELE PIWPVGFWDD WMRDPARRKD RQCIRPEISR
310 320 330 340 350
TGMMSYGKEG ASKGQFFSKH LAKIKVNDKY INFGKIDLDY LLPANFAKKT
360 370 380 390 400
NLEVMKEAVE LSIDNVASFV LSSENKGKSV RVMYDGNIDY IRKADKLHIM
410 420 430 440
HDFKAGVPRT AYDGIVTCFI NGIRIYLVPD RTKVSAYNPD WSVPPSFGE
Length:449
Mass (Da):52,151
Last modified:August 14, 2001 - v2
Checksum:i8448210B2A78FC88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082010 mRNA Translation: AAD03022.1
FO080200 Genomic DNA Translation: CCD61925.1
PIRiT43340
RefSeqiNP_509566.1, NM_077165.5
UniGeneiCel.19612

Genome annotation databases

EnsemblMetazoaiB0416.6; B0416.6; WBGene00001638
GeneIDi181160
KEGGicel:CELE_B0416.6
UCSCiB0416.6 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMGAT1_CAEEL
AccessioniPrimary (citable) accession number: Q11068
Secondary accession number(s): O76776
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 14, 2001
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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