Reviewed, UniProtKB/Swiss-Prot Q11067 (PDIA6_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable protein disulfide-isomerase A6
    EC=5.3.4.1

Gene names

Name:	tag-320
ORF Names:	B0403.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 440	422	Probable protein disulfide-isomerase A6		PRO_0000034241
Regions
	Domain	19 – 131	113	Thioredoxin 1
	Domain	127 – 273	147	Thioredoxin 2
	Motif	437 – 440	4	Prevents secretion from ER By similarity
	Compositional bias	142 – 152	11	Poly-Ser
	Compositional bias	158 – 163	6	Poly-Gly
	Compositional bias	425 – 436	12	Asp/Glu-rich (acidic)
Sites
	Active site	54	1	Nucleophile By similarity
	Active site	57	1	Nucleophile By similarity
	Active site	194	1	Nucleophile By similarity
	Active site	197	1	Nucleophile By similarity
	Site	55	1	Contributes to redox potential value By similarity
	Site	56	1	Contributes to redox potential value By similarity
	Site	117	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	195	1	Contributes to redox potential value By similarity
	Site	196	1	Contributes to redox potential value By similarity
	Site	259	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Disulfide bond	54 ↔ 57		Redox-active By similarity
	Disulfide bond	194 ↔ 197		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11067-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 608785076B159578	FASTA	440	47,728
	10 20 30 40 50 60 MALIKLLLAS LAITSVCGMY SKKDDVVELT EANFQSKVIN SDDIWIVEFY APWCGHCKSL 70 80 90 100 110 120 VPEYKKAASA LKGVAKVGAV DMTQHQSVGG PYNVQGFPTL KIFGADKKKP TDYNGQRTAQ 130 140 150 160 170 180 AIADSVLAEA KKAVSARLGG KSSGSSSSGS GSGSGKRGGG GSGNEVVELT DANFEDLVLN 190 200 210 220 230 240 SKDIWLVEFF APWCGHCKSL EPQWKAAASE LKGKVRLGAL DATVHTVVAN KFAIRGFPTI 250 260 270 280 290 300 KYFAPGSDVS DAQDYDGGRQ SSDIVAWASA RAQENMPAPE VFEGINQQVV EDACKEKQLC 310 320 330 340 350 360 IFAFLPHILD CQSECRNNYL AMLKEQSEKF KKNLWGWIWV EGAAQPALEE SFEVGGFGYP 370 380 390 400 410 420 AMTALNFRKN KYAVLKGSFG KDGIHEFLRD LSYGKGRTSS LRGDGFPKIQ KTEKWDGKDG 430 440 ALPAEDDIDL SDIDLDKTEL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40411 Genomic DNA. Translation: AAC47065.1.
PIR	T15352.
RefSeq	NP_509190.1.
UniGene	Cel.6685
3D structure databases
HSSP	HSSP built from PDB template 1MEK based on UniProtKB P07237.
ModBase	Search...
Genome annotation databases
Ensembl	B0403.4. Caenorhabditis elegans. [Contig view]
GeneID	180974.
KEGG	cel:B0403.4.
NMPDR	fig|6239.3.peg.23679.
Organism-specific databases
WormBase	WBGene00015168. tag-320.
WormPep	B0403.4. CE03880. [WorfDB]
Phylogenomic databases
OMA	Q11067. LEPEWAA.
Enzyme and pathway databases
BRENDA	5.3.4.1. 672.
Gene expression databases
ArrayExpress	Q11067.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	911804.

Entry information

Entry name

PDIA6_CAEEL

Accession

Primary (citable) accession number: Q11067

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project