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Protein

Puromycin-sensitive aminopeptidase

Gene

Npepps

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins.1 Publication

Catalytic activityi

Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Strongly inhibited by bestatin, leuhistin, actinonin, amastatin, 1,10-phenanthroline, DFP, PCMBS, Zn2+, Cd2+, Co2+, Cu2+, Hg2+, EDTA and puromycin. Not inhibited by PMSF, and only slightly inhibited by leupeptin and aprotinin. Activity is increased by Mg2+ and Ca2+ (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181SubstrateBy similarity1
Metal bindingi353Zinc; catalyticPROSITE-ProRule annotation1
Active sitei354Proton acceptorPROSITE-ProRule annotation1
Metal bindingi357Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi376Zinc; catalyticPROSITE-ProRule annotation1
Sitei439Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM01.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Puromycin-sensitive aminopeptidase (EC:3.4.11.14)
Short name:
PSA
Alternative name(s):
Cytosol alanyl aminopeptidase
Short name:
AAP-S
Gene namesi
Name:Npepps
Synonyms:Psa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1101358. Npepps.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit dwarfism, increased anxiety, impaired pain responses and do not reproduce as well as wild-type mice. More MHC class I molecules are displayed on dendritic cell surfaces.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000951171 – 920Puromycin-sensitive aminopeptidaseAdd BLAST920

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei465Nitrated tyrosineCombined sources1

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ11011.
MaxQBiQ11011.
PaxDbiQ11011.
PeptideAtlasiQ11011.
PRIDEiQ11011.

PTM databases

iPTMnetiQ11011.
PhosphoSitePlusiQ11011.
SwissPalmiQ11011.

Expressioni

Tissue specificityi

Widely expressed. Highest expression in brain, particularly the striatum and hippocampus. Expressed in Sertoli cells.3 Publications

Gene expression databases

BgeeiENSMUSG00000001441.
CleanExiMM_NPEPPS.
ExpressionAtlasiQ11011. baseline and differential.
GenevisibleiQ11011. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ11011. 2 interactors.
MINTiMINT-4108858.
STRINGi10090.ENSMUSP00000001480.

Structurei

3D structure databases

ProteinModelPortaliQ11011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 321Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi727 – 731Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
HOVERGENiHBG106325.
InParanoidiQ11011.
KOiK08776.
OMAiRTVQQCC.
OrthoDBiEOG091G01GH.
PhylomeDBiQ11011.
TreeFamiTF300395.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q11011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLAAAVPSL ARRLLLLGPP PPPLLLLLSR SSRRRRRLHS LGLAAMPEKR
60 70 80 90 100
PFERLPAEVS PINYSLCLKP DLLDFTFEGK LEAAAQVRQA TNQIVMNCAD
110 120 130 140 150
IDIITASYAP EGDEEIHATG FNYQNEDEKV TLSFPSTLQT GTGTLKIDFV
160 170 180 190 200
GELNDKMKGF YRSRYTTPAG EVRYAAVTQF EATDARRAFP CWDEPAIKAT
210 220 230 240 250
FDISLVVPKD RVALSNMNVI DRKPYPDDEN LVEVKFARTP VMSTYLVAFV
260 270 280 290 300
VGEYDFVETR SKDGVCVRVY TPVGKAEQGK FALEVAAKTL PFYKDYFNVP
310 320 330 340 350
YPLPKIDLIA IADFAAGAME NWGLVTYRET ALLIDPKNSC SSSRQWVALV
360 370 380 390 400
VGHELAHQWF GNLVTMEWWT HLWLNEGFAS WIEYLCVDHC FPEYDIWTQF
410 420 430 440 450
VSADYTRAQE LDALDNSHPI EVSVGHPSEV DEIFDAISYS KGASVIRMLH
460 470 480 490 500
DYIGDKDFKK GMNMYLTKFQ QKNAATEDLW ESLESASGKP IAAVMNTWTK
510 520 530 540 550
QMGFPLIYVE AEQVEDDRVL KLSQKKFCAS GPYGGEDCPQ WMVPITISTS
560 570 580 590 600
EDPNQAKLKI LMDKPEMSVV LKNVKPDQWV KLNLGTVGFY RTQYSSAMLE
610 620 630 640 650
SLLPGIRDLS LPPVDRLGLQ NDLFSLARAG IISTVEVLKV MEAFVNEPNY
660 670 680 690 700
TVWSDLSCNL GILSTLLSHT DFYEEIQEFV KDVFSPIGER LGWDPKPGEG
710 720 730 740 750
HLDALLRGLV LGKLGKAGHK ATLEEARRRF KEHVEGKQIL SADLRSPVYL
760 770 780 790 800
TVLKHGDGAT LDIMLKLHKQ ADMQEEKNRI ERVLGATLSP ELIQKVLTFA
810 820 830 840 850
LSEEVRPQDT VSVIGGVAGG SKHGRKAAWK FIKDNWEELH NRYQGGFLIS
860 870 880 890 900
RLIKLSVEGF AVDKMAGEVK AFFESHPAPS AERTIQQCCE NILLNAAWLK
910 920
RDADSIHQYL LQRKTSPPSV
Length:920
Mass (Da):103,325
Last modified:July 13, 2010 - v2
Checksum:iBECF4139F074A356
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185A → P in AAC52409 (PubMed:7592939).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35646 mRNA. Translation: AAC52409.1.
AL627445 Genomic DNA. Translation: CAM26998.1.
CH466556 Genomic DNA. Translation: EDL16075.1.
BC009653 mRNA. Translation: AAH09653.1.
BC086798 mRNA. Translation: AAH86798.1.
BC098212 mRNA. Translation: AAH98212.1.
AK133898 mRNA. Translation: BAE21917.1.
CCDSiCCDS25317.1.
PIRiT10052.
RefSeqiNP_032968.2. NM_008942.2.
UniGeneiMm.29824.

Genome annotation databases

EnsembliENSMUST00000001480; ENSMUSP00000001480; ENSMUSG00000001441.
GeneIDi19155.
KEGGimmu:19155.
UCSCiuc007ldv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35646 mRNA. Translation: AAC52409.1.
AL627445 Genomic DNA. Translation: CAM26998.1.
CH466556 Genomic DNA. Translation: EDL16075.1.
BC009653 mRNA. Translation: AAH09653.1.
BC086798 mRNA. Translation: AAH86798.1.
BC098212 mRNA. Translation: AAH98212.1.
AK133898 mRNA. Translation: BAE21917.1.
CCDSiCCDS25317.1.
PIRiT10052.
RefSeqiNP_032968.2. NM_008942.2.
UniGeneiMm.29824.

3D structure databases

ProteinModelPortaliQ11011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ11011. 2 interactors.
MINTiMINT-4108858.
STRINGi10090.ENSMUSP00000001480.

Protein family/group databases

MEROPSiM01.010.

PTM databases

iPTMnetiQ11011.
PhosphoSitePlusiQ11011.
SwissPalmiQ11011.

Proteomic databases

EPDiQ11011.
MaxQBiQ11011.
PaxDbiQ11011.
PeptideAtlasiQ11011.
PRIDEiQ11011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001480; ENSMUSP00000001480; ENSMUSG00000001441.
GeneIDi19155.
KEGGimmu:19155.
UCSCiuc007ldv.2. mouse.

Organism-specific databases

CTDi9520.
MGIiMGI:1101358. Npepps.

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
HOVERGENiHBG106325.
InParanoidiQ11011.
KOiK08776.
OMAiRTVQQCC.
OrthoDBiEOG091G01GH.
PhylomeDBiQ11011.
TreeFamiTF300395.

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiNpepps. mouse.
PROiQ11011.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001441.
CleanExiMM_NPEPPS.
ExpressionAtlasiQ11011. baseline and differential.
GenevisibleiQ11011. MM.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSA_MOUSE
AccessioniPrimary (citable) accession number: Q11011
Secondary accession number(s): Q3UZE0, Q5PR74, Q91VJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 13, 2010
Last modified: November 2, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-46 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.