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Reviewed, UniProtKB/Swiss-Prot Q11011 (PSA_MOUSE)

Last modified June 16, 2009. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Puromycin-sensitive aminopeptidase
      Short name=PSA
    EC=3.4.11.-
Gene names
Name: Npepps
Synonyms: Psa
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Aminopeptidase with broad substrate specificity to several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Cytoplasm. Nucleus.

Tissue specificity

Widely expressed. Highest expression in brain.

Sequence similarities

Belongs to the peptidase M1 family.

Caution

It is uncertain whether Met-1 or Met-46 is the initiator.

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Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   LigandMetal-binding
Zinc
   Molecular functionAminopeptidase
Hydrolase
Metalloprotease
Protease
   PTMNitration
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metallopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 920920Puromycin-sensitive aminopeptidase
PRO_0000095117

Regions

Motif727 – 7315Nuclear localization signal Potential

Sites

Active site3541 By similarity
Active site4051Proton donor Potential
Metal binding3531Zinc; catalytic By similarity
Metal binding3571Zinc; catalytic By similarity
Metal binding3761Zinc; catalytic By similarity

Amino acid modifications

Modified residue4651Nitrated tyrosine

Experimental info

Sequence conflict1851P → A in AAH09653. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q11011-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 30D4378C3969628B

FASTA920103,351
        10         20         30         40         50         60 
MWLAAAVPSL ARRLLLLGPP PPPLLLLLSR SSRRRRRLHS LGLAAMPEKR PFERLPAEVS 

        70         80         90        100        110        120 
PINYSLCLKP DLLDFTFEGK LEAAAQVRQA TNQIVMNCAD IDIITASYAP EGDEEIHATG 

       130        140        150        160        170        180 
FNYQNEDEKV TLSFPSTLQT GTGTLKIDFV GELNDKMKGF YRSRYTTPAG EVRYAAVTQF 

       190        200        210        220        230        240 
EATDPRRAFP CWDEPAIKAT FDISLVVPKD RVALSNMNVI DRKPYPDDEN LVEVKFARTP 

       250        260        270        280        290        300 
VMSTYLVAFV VGEYDFVETR SKDGVCVRVY TPVGKAEQGK FALEVAAKTL PFYKDYFNVP 

       310        320        330        340        350        360 
YPLPKIDLIA IADFAAGAME NWGLVTYRET ALLIDPKNSC SSSRQWVALV VGHELAHQWF 

       370        380        390        400        410        420 
GNLVTMEWWT HLWLNEGFAS WIEYLCVDHC FPEYDIWTQF VSADYTRAQE LDALDNSHPI 

       430        440        450        460        470        480 
EVSVGHPSEV DEIFDAISYS KGASVIRMLH DYIGDKDFKK GMNMYLTKFQ QKNAATEDLW 

       490        500        510        520        530        540 
ESLESASGKP IAAVMNTWTK QMGFPLIYVE AEQVEDDRVL KLSQKKFCAS GPYGGEDCPQ 

       550        560        570        580        590        600 
WMVPITISTS EDPNQAKLKI LMDKPEMSVV LKNVKPDQWV KLNLGTVGFY RTQYSSAMLE 

       610        620        630        640        650        660 
SLLPGIRDLS LPPVDRLGLQ NDLFSLARAG IISTVEVLKV MEAFVNEPNY TVWSDLSCNL 

       670        680        690        700        710        720 
GILSTLLSHT DFYEEIQEFV KDVFSPIGER LGWDPKPGEG HLDALLRGLV LGKLGKAGHK 

       730        740        750        760        770        780 
ATLEEARRRF KEHVEGKQIL SADLRSPVYL TVLKHGDGAT LDIMLKLHKQ ADMQEEKNRI 

       790        800        810        820        830        840 
ERVLGATLSP ELIQKVLTFA LSEEVRPQDT VSVIGGVAGG SKHGRKAAWK FIKDNWEELH 

       850        860        870        880        890        900 
NRYQGGFLIS RLIKLSVEGF AVDKMAGEVK AFFESHPAPS AERTIQQCCE NILLNAAWLK 

       910        920 
RDADSIHQYL LQRKTSPPSV

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References

« Hide 'large scale' references
[1]"Puromycin-sensitive aminopeptidase. Sequence analysis, expression, and functional characterization."
Constam D.B., Tobler A.R., Rensing-Ehl A., Kemler I., Hersh L.B., Fontana A.
J. Biol. Chem. 270:26931-26939(1995) [PubMed: 7592939] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[3]"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease."
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J.
Biochemistry 45:8009-8022(2006) [PubMed: 16800626] [Abstract]
Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-465, MASS SPECTROMETRY.
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U35646 mRNA. Translation: AAC52409.1.
BC009653 mRNA. Translation: AAH09653.1.
IPIIPI00130000.
PIRT10052.
UniGeneMm.29824

3D structure databases

ModBaseSearch...

Protein family/group databases

MEROPSM01.010.

PTM databases

PhosphoSiteQ11011.

Proteomic databases

PRIDEQ11011.

Genome annotation databases

EnsemblENSMUSG00000001441. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:1101358. Npepps.

Phylogenomic databases

HOGENOMQ11011.
HOVERGENQ11011.

Gene expression databases

ArrayExpressQ11011.
BgeeQ11011.
CleanExMM_NPEPPS.
GermOnlineENSMUSG00000001441. Mus musculus.

Family and domain databases

InterProIPR006025. Pept_M_Zn_BS.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR015568. Peptidase_M1_puromycin-sens.
[Graphical view]
PANTHERPTHR11533:SF33. Pept_M1_puromysin-sens. 1 hit.
PTHR11533. Peptidase_M1. 1 hit.
PfamPF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry namePSA_MOUSE
AccessionPrimary (citable) accession number: Q11011
Secondary accession number(s): Q91VJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents