Q11010 (AMPN_STRLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminopeptidase N
EC=3.4.11.2

Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name=Lys-AP

Gene names

Name:

pepN

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aminopeptidase with broad substrate specificity to several peptides. Shows strong preference for leucine but cleaves also next to Arg and lysine in peptide-bond-containing substrates.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 857	857	Aminopeptidase N		PRO_0000095076
Regions
	Region	264 – 268	5	Substrate binding By similarity
Sites
	Active site	299	1	Proton acceptor By similarity
	Metal binding	298	1	Zinc; catalytic By similarity
	Metal binding	302	1	Zinc; catalytic By similarity
	Metal binding	321	1	Zinc; catalytic By similarity
	Binding site	130	1	Substrate By similarity
	Site	386	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q11010 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 40B749F78D201A7D	FASTA	857	94,424
	10 20 30 40 50 60 MPGTNLTREE ARQRATLLTV DSYEIDLDLT GAQEGGTYRS VTTVRFDVAE GGGESFIDLV 70 80 90 100 110 120 APTVHEVTLN GDALDTAEVF QDSRIALPGL LPGRNILRVV ADCAYTNTGE GLHRFVDPVD 130 140 150 160 170 180 DQAYLYTQFE VPDARRVFAS FEQPDLKATF QFTVKAPEGW TVISNSPTPE PKDNVWEFEP 190 200 210 220 230 240 TPRISSYVTA LIVGPYHSVH SVYEKDGQSV PLGIYCRPSL AEHLDADAIF EVTRQGFDWF 250 260 270 280 290 300 QEKFDYAYPF KKYDQLFVPE FNAGAMENAG AVTIRDQYVF RSKVTDAAYE VRAATILHEL 310 320 330 340 350 360 AHMWFGDLVT MEWWNDLWLN ESFATYAEAA CQAAAPGSKW PHSWTTFANQ MKTWAYRQDQ 370 380 390 400 410 420 LPSTHPIMAD ISDLDDVLVN FDGITYAKGA SVLKQLVAYV GEEAFFKGVQ AYFKRHAFGN 430 440 450 460 470 480 TRLSDLLGAL EETSGRDLKT WSKAWLETAG INVLRPEIET DADGVITSFA IRQEAPALPA 490 500 510 520 530 540 GAKGEPTLRP HRIAIGAYDL DGAGKLVRGD RVELDVDGEL TAVPQLVGKA RPAVLLLNDD 550 560 570 580 590 600 DLSYAKVRLD EQSLAVVTEH LGDFTESLPR ALCWASAWDM TRDAELATRD YLALVLSGIG 610 620 630 640 650 660 KESDIGVVQS LHRQVKLAID QYAAPTAREA LLTRWTEATL AHLRAAEAGS DHQLAWARAF 670 680 690 700 710 720 AATARTPEQL DLLDALLDGT QTIEGLAVDT ELRWAFVQRL AAVGRFGGSE IAAEYERDKT 730 740 750 760 770 780 AAGERHAATA RAARPTEAAK AEAWESVVES DKLPNAVQEA VIAGFVQTDQ RELLAAYTER 790 800 810 820 830 840 YFEALKDVWA SRSHEMAQQI AVGLYPAVQV SQDTLDRTDA WLASAEPNAA LRRLVSESRS 850 GIERALRAQA ADAAAAE

References

[1]	"The aminopeptidase N-encoding pepN gene of Streptomyces lividans 66." Butler M.J., Aphale J.S., Binnie C., Dizonno M.A., Krygsman P., Soltes G.A., Walczyk E., Malek L.T. Gene 141:115-119(1994) [PubMed: 7909302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 415-439. Strain: 66 / 1326.
[2]	"Intracellular aminopeptidases in Streptomyces lividans 66." Butler M.J., Aphale J.S., Dizonno M.A., Krygsman P., Walczyk E., Malek L.T. J. Ind. Microbiol. 13:24-29(1994) [PubMed: 7765336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 66 / 1326.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23172 Genomic DNA. Translation: AAA26696.1.
3D structure databases
ProteinModelPortal	Q11010.
ModBase	Search...
Protein family/group databases
MEROPS	M01.009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012778. Pept_M1_aminopeptidase. IPR024571. Pept_M1_aminopeptidase_C. IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF11838. DUF3358. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02412. PepN_strep_liv. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_STRLI

Accession

Primary (citable) accession number: Q11010

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program