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Protein

Aminopeptidase N

Gene

pepN

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. Shows strong preference for leucine but cleaves also next to Arg and lysine in peptide-bond-containing substrates.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130SubstrateBy similarity1
Metal bindingi298Zinc; catalyticPROSITE-ProRule annotation1
Active sitei299Proton acceptorPROSITE-ProRule annotation1
Metal bindingi302Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi321Zinc; catalyticPROSITE-ProRule annotation1
Sitei386Transition state stabilizerBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950761 – 857Aminopeptidase NAdd BLAST857

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ11010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 268Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR012778. Pept_M1_aminopeptidase.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 2 hits.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
TIGRFAMsiTIGR02412. pepN_strep_liv. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q11010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTNLTREE ARQRATLLTV DSYEIDLDLT GAQEGGTYRS VTTVRFDVAE
60 70 80 90 100
GGGESFIDLV APTVHEVTLN GDALDTAEVF QDSRIALPGL LPGRNILRVV
110 120 130 140 150
ADCAYTNTGE GLHRFVDPVD DQAYLYTQFE VPDARRVFAS FEQPDLKATF
160 170 180 190 200
QFTVKAPEGW TVISNSPTPE PKDNVWEFEP TPRISSYVTA LIVGPYHSVH
210 220 230 240 250
SVYEKDGQSV PLGIYCRPSL AEHLDADAIF EVTRQGFDWF QEKFDYAYPF
260 270 280 290 300
KKYDQLFVPE FNAGAMENAG AVTIRDQYVF RSKVTDAAYE VRAATILHEL
310 320 330 340 350
AHMWFGDLVT MEWWNDLWLN ESFATYAEAA CQAAAPGSKW PHSWTTFANQ
360 370 380 390 400
MKTWAYRQDQ LPSTHPIMAD ISDLDDVLVN FDGITYAKGA SVLKQLVAYV
410 420 430 440 450
GEEAFFKGVQ AYFKRHAFGN TRLSDLLGAL EETSGRDLKT WSKAWLETAG
460 470 480 490 500
INVLRPEIET DADGVITSFA IRQEAPALPA GAKGEPTLRP HRIAIGAYDL
510 520 530 540 550
DGAGKLVRGD RVELDVDGEL TAVPQLVGKA RPAVLLLNDD DLSYAKVRLD
560 570 580 590 600
EQSLAVVTEH LGDFTESLPR ALCWASAWDM TRDAELATRD YLALVLSGIG
610 620 630 640 650
KESDIGVVQS LHRQVKLAID QYAAPTAREA LLTRWTEATL AHLRAAEAGS
660 670 680 690 700
DHQLAWARAF AATARTPEQL DLLDALLDGT QTIEGLAVDT ELRWAFVQRL
710 720 730 740 750
AAVGRFGGSE IAAEYERDKT AAGERHAATA RAARPTEAAK AEAWESVVES
760 770 780 790 800
DKLPNAVQEA VIAGFVQTDQ RELLAAYTER YFEALKDVWA SRSHEMAQQI
810 820 830 840 850
AVGLYPAVQV SQDTLDRTDA WLASAEPNAA LRRLVSESRS GIERALRAQA

ADAAAAE
Length:857
Mass (Da):94,424
Last modified:October 1, 1996 - v1
Checksum:i40B749F78D201A7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23172 Genomic DNA. Translation: AAA26696.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23172 Genomic DNA. Translation: AAA26696.1.

3D structure databases

ProteinModelPortaliQ11010.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM01.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR012778. Pept_M1_aminopeptidase.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 2 hits.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
TIGRFAMsiTIGR02412. pepN_strep_liv. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPN_STRLI
AccessioniPrimary (citable) accession number: Q11010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.