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Q11010

- AMPN_STRLI

UniProt

Q11010 - AMPN_STRLI

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Protein
Aminopeptidase N
Gene
pepN
Organism
Streptomyces lividans
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. Shows strong preference for leucine but cleaves also next to Arg and lysine in peptide-bond-containing substrates.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei130 – 1301Substrate By similarity
Metal bindingi298 – 2981Zinc; catalytic By similarity
Active sitei299 – 2991Proton acceptor By similarity
Metal bindingi302 – 3021Zinc; catalytic By similarity
Metal bindingi321 – 3211Zinc; catalytic By similarity
Sitei386 – 3861Transition state stabilizer By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Alternative name(s):
    Alanine aminopeptidase
    Lysyl aminopeptidase
    Short name:
    Lys-AP
    Gene namesi
    Name:pepN
    OrganismiStreptomyces lividans
    Taxonomic identifieri1916 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 857857Aminopeptidase N
    PRO_0000095076Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ11010.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni264 – 2685Substrate binding By similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR012778. Pept_M1_aminopeptidase.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    TIGRFAMsiTIGR02412. pepN_strep_liv. 1 hit.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q11010-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGTNLTREE ARQRATLLTV DSYEIDLDLT GAQEGGTYRS VTTVRFDVAE    50
    GGGESFIDLV APTVHEVTLN GDALDTAEVF QDSRIALPGL LPGRNILRVV 100
    ADCAYTNTGE GLHRFVDPVD DQAYLYTQFE VPDARRVFAS FEQPDLKATF 150
    QFTVKAPEGW TVISNSPTPE PKDNVWEFEP TPRISSYVTA LIVGPYHSVH 200
    SVYEKDGQSV PLGIYCRPSL AEHLDADAIF EVTRQGFDWF QEKFDYAYPF 250
    KKYDQLFVPE FNAGAMENAG AVTIRDQYVF RSKVTDAAYE VRAATILHEL 300
    AHMWFGDLVT MEWWNDLWLN ESFATYAEAA CQAAAPGSKW PHSWTTFANQ 350
    MKTWAYRQDQ LPSTHPIMAD ISDLDDVLVN FDGITYAKGA SVLKQLVAYV 400
    GEEAFFKGVQ AYFKRHAFGN TRLSDLLGAL EETSGRDLKT WSKAWLETAG 450
    INVLRPEIET DADGVITSFA IRQEAPALPA GAKGEPTLRP HRIAIGAYDL 500
    DGAGKLVRGD RVELDVDGEL TAVPQLVGKA RPAVLLLNDD DLSYAKVRLD 550
    EQSLAVVTEH LGDFTESLPR ALCWASAWDM TRDAELATRD YLALVLSGIG 600
    KESDIGVVQS LHRQVKLAID QYAAPTAREA LLTRWTEATL AHLRAAEAGS 650
    DHQLAWARAF AATARTPEQL DLLDALLDGT QTIEGLAVDT ELRWAFVQRL 700
    AAVGRFGGSE IAAEYERDKT AAGERHAATA RAARPTEAAK AEAWESVVES 750
    DKLPNAVQEA VIAGFVQTDQ RELLAAYTER YFEALKDVWA SRSHEMAQQI 800
    AVGLYPAVQV SQDTLDRTDA WLASAEPNAA LRRLVSESRS GIERALRAQA 850
    ADAAAAE 857
    Length:857
    Mass (Da):94,424
    Last modified:October 1, 1996 - v1
    Checksum:i40B749F78D201A7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23172 Genomic DNA. Translation: AAA26696.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23172 Genomic DNA. Translation: AAA26696.1 .

    3D structure databases

    ProteinModelPortali Q11010.
    ModBasei Search...

    Protein family/group databases

    MEROPSi M01.009.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR012778. Pept_M1_aminopeptidase.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    TIGRFAMsi TIGR02412. pepN_strep_liv. 1 hit.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The aminopeptidase N-encoding pepN gene of Streptomyces lividans 66."
      Butler M.J., Aphale J.S., Binnie C., Dizonno M.A., Krygsman P., Soltes G.A., Walczyk E., Malek L.T.
      Gene 141:115-119(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 415-439.
      Strain: 66 / 1326.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 66 / 1326.

    Entry informationi

    Entry nameiAMPN_STRLI
    AccessioniPrimary (citable) accession number: Q11010
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: May 14, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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