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Q10YQ3 (BIOB_TRIEI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Tery_3533
OrganismTrichodesmium erythraeum (strain IMS101) [Complete proteome] [HAMAP]
Taxonomic identifier203124 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesTrichodesmium

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Biotin synthase HAMAP-Rule MF_01694
PRO_0000381692

Sites

Metal binding971Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1011Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1041Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1791Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2391Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3091Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q10YQ3 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: CC8C87B15288A197

FASTA37039,818
        10         20         30         40         50         60 
MIAVLKTPLS NHIKANTKAL FSSLPKTKNE LEISLDDLAK QIISGYRLSR EEAIILTQIA 

        70         80         90        100        110        120 
GEEDILLLCE AADRVRQACC GNVVDLCSII NVKSGGCSEN CSFCSQSVHH PGEDSPIYGL 

       130        140        150        160        170        180 
KSTEEILDQA KAAEAAGAKR FCLVSQGRGV KYNSPKSTEF EQILGTVRQI LAETNIKPCC 

       190        200        210        220        230        240 
ALGELTPEQA QALAEAGVTR YNHNLEASEH FYPEIVSTHS WNDRVETVKN LKAAGIQACT 

       250        260        270        280        290        300 
GGIIGMGETW ADRIDLALAL RELEVESVPL NLLNSREGTP LGGLLKLDPY DALKAIAIFR 

       310        320        330        340        350        360 
LILPKQIIRY AGGREAVMGD LQALGLKAGI NAMLIGHYLT TLGQPPEKDQ AMLKSLGLQG 

       370 
GETPIATNGL 

« Hide

References

[1]"Complete sequence of Trichodesmium erythraeum IMS101."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IMS101.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000393 Genomic DNA. Translation: ABG52621.1.
RefSeqYP_723094.1. NC_008312.1.

3D structure databases

ProteinModelPortalQ10YQ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203124.Tery_3533.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG52621; ABG52621; Tery_3533.
GeneID4244359.
KEGGter:Tery_3533.
PATRIC23992168. VBITriEry99848_4499.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycTERY203124:GJDR-3567-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_TRIEI
AccessionPrimary (citable) accession number: Q10YQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways