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Q10YQ3

- BIOB_TRIEI

UniProt

Q10YQ3 - BIOB_TRIEI

Protein

Biotin synthase

Gene

bioB

Organism
Trichodesmium erythraeum (strain IMS101)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi97 – 971Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi179 – 1791Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi239 – 2391Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi309 – 3091Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciTERY203124:GJDR-3567-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Tery_3533
    OrganismiTrichodesmium erythraeum (strain IMS101)
    Taxonomic identifieri203124 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesTrichodesmium
    ProteomesiUP000008878: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Biotin synthasePRO_0000381692Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203124.Tery_3533.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10YQ3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q10YQ3-1 [UniParc]FASTAAdd to Basket

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    MIAVLKTPLS NHIKANTKAL FSSLPKTKNE LEISLDDLAK QIISGYRLSR    50
    EEAIILTQIA GEEDILLLCE AADRVRQACC GNVVDLCSII NVKSGGCSEN 100
    CSFCSQSVHH PGEDSPIYGL KSTEEILDQA KAAEAAGAKR FCLVSQGRGV 150
    KYNSPKSTEF EQILGTVRQI LAETNIKPCC ALGELTPEQA QALAEAGVTR 200
    YNHNLEASEH FYPEIVSTHS WNDRVETVKN LKAAGIQACT GGIIGMGETW 250
    ADRIDLALAL RELEVESVPL NLLNSREGTP LGGLLKLDPY DALKAIAIFR 300
    LILPKQIIRY AGGREAVMGD LQALGLKAGI NAMLIGHYLT TLGQPPEKDQ 350
    AMLKSLGLQG GETPIATNGL 370
    Length:370
    Mass (Da):39,818
    Last modified:August 22, 2006 - v1
    Checksum:iCC8C87B15288A197
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000393 Genomic DNA. Translation: ABG52621.1.
    RefSeqiYP_723094.1. NC_008312.1.

    Genome annotation databases

    EnsemblBacteriaiABG52621; ABG52621; Tery_3533.
    GeneIDi4244359.
    KEGGiter:Tery_3533.
    PATRICi23992168. VBITriEry99848_4499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000393 Genomic DNA. Translation: ABG52621.1 .
    RefSeqi YP_723094.1. NC_008312.1.

    3D structure databases

    ProteinModelPortali Q10YQ3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203124.Tery_3533.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG52621 ; ABG52621 ; Tery_3533 .
    GeneIDi 4244359.
    KEGGi ter:Tery_3533.
    PATRICi 23992168. VBITriEry99848_4499.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci TERY203124:GJDR-3567-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IMS101.

    Entry informationi

    Entry nameiBIOB_TRIEI
    AccessioniPrimary (citable) accession number: Q10YQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3