Q10WA8 (ISPH_TRIEI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Tery_4479

Organism

Trichodesmium erythraeum (strain IMS101) [Complete proteome] [HAMAP]

Taxonomic identifier

203124 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Trichodesmium

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021192

Sequences

	Sequence		Length	Mass (Da)
	Q10WA8 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 9D3F542C02B1B994	FASTA	401	45,425
	10 20 30 40 50 60 MDTKAFTRAL KKSENYNRQG FGHAQEVATL MQSAYKSNLI QQIRENNYTL QRGEVTIKLA 70 80 90 100 110 120 KTFGFCWGVE RSVAMAYETR QHFPNQQIWI SYELIHNPSV NQDMKDMEIK FIPVIDGQKD 130 140 150 160 170 180 FSVAGNNDVV ILPAFGASVQ EMQILKDKNC QIVDTTCPWV SKVWNSIEKH KKKKFTSIIH 190 200 210 220 230 240 GKYSHAETIA TSSFADKYLI VLNMTQAQYV CNYILNGGNR DEFLAKFKQA YSEGFDPDLD 250 260 270 280 290 300 LEQVGIANQT TMLKTETEEI GKLFERTMMK KYRPDQLNAH YQNFNTICDA TQERQDAIAG 310 320 330 340 350 360 LFNEKLDLMV VIGGFNSSNT THLQEMAIEN GIPSYHIDGA QRILSRNKIE HKPLGREVKI 370 380 390 400 SEGWLPEGPV VVGLTSGAST PDQAVEDTIN KIVAMKLVVM N

References

[1]

"Complete sequence of Trichodesmium erythraeum IMS101." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IMS101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000393 Genomic DNA. Translation: ABG53466.1.
RefSeq	YP_723939.1. NC_008312.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q10WA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4246132.
GenomeReviews	Gene locus Tery_4479 in contig CP000393_GR.
KEGG	ter:Tery_4479.
NMPDR	fig|203124.1.peg.3223.
PATRIC	23994531. VBITriEry99848_5668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	Q10WA8.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	TERY203124:TERY_4479-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_TRIEI

Accession

Primary (citable) accession number: Q10WA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program