Q10VG8 (SYA_TRIEI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Tery_4806

Organism

Trichodesmium erythraeum (strain IMS101) [Complete proteome] [HAMAP]

Taxonomic identifier

203124 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Trichodesmium

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347855
Sites
	Metal binding	561	1	Zinc Potential
	Metal binding	565	1	Zinc Potential
	Metal binding	663	1	Zinc Potential
	Metal binding	667	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10VG8 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 65D22B5C5CE87B88	FASTA	874	96,282
	10 20 30 40 50 60 MSAKKLTGNE IRKKFLSFYA QREHTILPSA SLVPEDPTVL LTIAGMLPFK PIFLGQQPRQ 70 80 90 100 110 120 YPRATTSQKC IRTNDIENVG RTARHHTFFE MLGNFSFGDY FKPEAIALAW ELSTKIFALP 130 140 150 160 170 180 PERLVVSVFR EDDEAFAIWR DQIGIPAHRI QRMDEADNFW ASGPTGPCGP CSEIYYDFHP 190 200 210 220 230 240 ELGDDHIDLE DDTRFIEFYN LVFMQYNRDA NGNLTPLENR NIDTGMGLER MAQILQKVPN 250 260 270 280 290 300 NYETDLIFPI IKTASDIADI DYRKSDDKTK VSLKVIGDHV RAVANLIADG VTASNVGRGY 310 320 330 340 350 360 ILRRLIRRVV RHGRLIGISG EFTSKVAETA ITLAEDIYPN LRERETVIKA ELQREESRFL 370 380 390 400 410 420 ETLERGEKLL AEIMAKPETK KTQHISGEDA FKLYDTYGFP LELTQEIAEE NGLTVDVSMF 430 440 450 460 470 480 DQEMKLAQIR SQSAHETIDL TAQDGVKLDI DKTQFLGYTD LSSPAQVMAL VGDGEQLETV 490 500 510 520 530 540 QAGAQVQIVL DKTPFYAESG GQIADRGYLS GDSLVVRIED VQKQNNIFVH FGRIERGRLQ 550 560 570 580 590 600 LGMTVNAQID GTCRRRAQAN HTATHLLQAA LRSLVDSSIS QAGSLVSFDR LRFDFNCPRG 610 620 630 640 650 660 LKPEEVEQVE AQVNSWIAEA HSATVAEMPL EVAKAKGAVA MFGEKYADVV RVVDYPGVSM 670 680 690 700 710 720 ELCGGTHVNN TAEIGVFKII SEAGISSGVR RIEAVAGLAV LDYLKVRDAV VKELSDRFKA 730 740 750 760 770 780 KPEELSERVS NLQQELKDSQ KQLEALKGEL AVAKSDQLLG NAETVGEFQI LVAEMPGVDA 790 800 810 820 830 840 EALKTAAERL QQKLDESAVV LGSAAEGKVS LVAAFSKSVN GKGLQAGKFI GGIAKICGGG 850 860 870 GGGRPNLAQA GGRDPSKLKE ALESAKEQLV DGLK

References

[1]

"Complete sequence of Trichodesmium erythraeum IMS101." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IMS101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000393 Genomic DNA. Translation: ABG53756.1.
RefSeq	YP_724229.1. NC_008312.1.
3D structure databases
ProteinModelPortal	Q10VG8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q10VG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4246460.
GenomeReviews	Gene locus Tery_4806 in contig CP000393_GR.
KEGG	ter:Tery_4806.
NMPDR	fig|203124.1.peg.1917.
PATRIC	23995387. VBITriEry99848_6095.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	GESKTDQ.
PhylomeDB	Q10VG8.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	TERY203124:TERY_4806-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_TRIEI

Accession

Primary (citable) accession number: Q10VG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program