Reviewed, UniProtKB/Swiss-Prot Q10S58 (SAT2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable serine acetyltransferase 2
    EC=2.3.1.30
Alternative name(s):
    OsSERAT3;1

Gene names

Name:	SAT2
Ordered Locus Names:	Os03g0133900, LOC_Os03g04140
ORF Names:	OJ1006F06.16

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
Subunit structure	Homomultimer By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.
Sequence caution	The sequence AAN05506.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis
Coding sequence diversity	Alternative splicing
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	serine O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q10S58-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q10S58-2)

The sequence of this isoform differs from the canonical sequence as follows:
     305-326: SMAVGNPAKVVGYKDKEDPSLT → REQNQGRRAGRGGRGDNMIMIK
     327-354: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Probable serine acetyltransferase 2		PRO_0000363659
Regions
	Compositional bias	30 – 39	10	Poly-Gly
Natural variations
	Alternative sequence	305 – 326	22	SMAVG…DPSLT → REQNQGRRAGRGGRGDNMIM IK in isoform 2.		VSP_036319
	Alternative sequence	327 – 354	28	Missing in isoform 2.		VSP_036320
Experimental info
	Sequence conflict	347	1	K → R in AK067089. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 64C4029A13CE6FB5	FASTA	354	37,517
	10 20 30 40 50 60 MTSCGCLVLE KVEDHGGEAA GRGRGRLAQG GGGGGGGCGS CAGEWRSRSE TMFPIYVMGS 70 80 90 100 110 120 SRASSAAAAR GIVDAAGDPI WEAVKSEAKS EAEKEPILSS FLYASVLSHD CLERALSFVL 130 140 150 160 170 180 ANRLEDPTLL ATQLIDIFND VMMNNKDIRR SIRLDAQAFK DRDPACAQYS WALLYLKGYH 190 200 210 220 230 240 SVQSYRIAHV LWNQGRKVLA LALQSRISEV FAVDIHPAAR IGEGILLDHG TGLVIGETAI 250 260 270 280 290 300 VGNWVSLMQG VTLGGTGKEN GDRHPKIGQG ALLGAGATIL GNINVGEGAM IAAGSLVLKD 310 320 330 340 350 VPPHSMAVGN PAKVVGYKDK EDPSLTMKHD ARRDYFEHVA VSFSDDKANG SVVK
	Isoform 2. Checksum: AE85DA31E5741179	FASTA	326	34,519
	10 20 30 40 50 60 MTSCGCLVLE KVEDHGGEAA GRGRGRLAQG GGGGGGGCGS CAGEWRSRSE TMFPIYVMGS 70 80 90 100 110 120 SRASSAAAAR GIVDAAGDPI WEAVKSEAKS EAEKEPILSS FLYASVLSHD CLERALSFVL 130 140 150 160 170 180 ANRLEDPTLL ATQLIDIFND VMMNNKDIRR SIRLDAQAFK DRDPACAQYS WALLYLKGYH 190 200 210 220 230 240 SVQSYRIAHV LWNQGRKVLA LALQSRISEV FAVDIHPAAR IGEGILLDHG TGLVIGETAI 250 260 270 280 290 300 VGNWVSLMQG VTLGGTGKEN GDRHPKIGQG ALLGAGATIL GNINVGEGAM IAAGSLVLKD 310 320 VPPHREQNQG RRAGRGGRGD NMIMIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC099399 Genomic DNA. Translation: AAN05506.1. Sequence problems. DP000009 Genomic DNA. Translation: ABF93836.1. DP000009 Genomic DNA. Translation: ABF93837.1. AP008209 Genomic DNA. Translation: BAF10793.1. AK067089 mRNA. No translation available.
RefSeq	NP_001048879.1.
UniGene	Os.17315
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4331528.
GenomeReviews	Gene locus SAT2 in contig AP008209_GR.
KEGG	osa:4331528.
Organism-specific databases
Gramene	Q8H8D0.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010493. Ser_AcTrfase_N. IPR005881. Ser_O-AcTrfase. [Graphical view]
Pfam	PF00132. Hexapep. 3 hits. PF06426. SATase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01172. cysE. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT2_ORYSJ

Accession

Primary (citable) accession number: Q10S58
Secondary accession number(s): Q10S57, Q8H8D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 22, 2006
Last modified:	September 22, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)