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Protein

Probable protein phosphatase 2C 28

Gene

Os03g0137200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871Manganese 1By similarity
Metal bindingi87 – 871Manganese 2By similarity
Metal bindingi88 – 881Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi288 – 2881Manganese 2By similarity
Metal bindingi347 – 3471Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 28 (EC:3.1.3.16)
Short name:
OsPP2C28
Gene namesi
Ordered Locus Names:Os03g0137200, LOC_Os03g04430
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10S32.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Probable protein phosphatase 2C 28PRO_0000363274Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiQ10S32. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g04430.1.

Structurei

3D structure databases

ProteinModelPortaliQ10S32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 328279PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000238703.
InParanoidiQ10S32.
OMAiDSNAMSK.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10S32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAAVMDYFR SCWGPRSPAG HRVRGSDVAG RQDGLLWYKD AGQLVTGEFS
60 70 80 90 100
MAVVQANNLL EDQSQVESGA LSMAEPGPQG TFIGVYDGHG GPETARFIND
110 120 130 140 150
HMFHHLRRFA TEHKCMSTDV IRKAFQATEE GFLSLVSKQW SLKPQIAAVG
160 170 180 190 200
SCCLVGVICS GTLYVANLGD SRAVLGRFVK STGEVVATQL SSEHNACYEE
210 220 230 240 250
VRQELQASHP DDPQIVVLKH NVWRVKGLIQ ISRSIGDVYL KRPEYNREPL
260 270 280 290 300
HSKFRLRETF KRPILSSEPA IAVHQIQPND HFVIFASDGL WEHLSNQEAV
310 320 330 340 350
DLVQNNPRNG IARRLVKVAM QEAAKKREMR YSDLKKIDRG VRRHFHDDIT
360 370 380 390
VIVVFLDSNA ISKANWSRGP SVSLRGGGVT LPANSLAPFS TPTVLSSTY
Length:399
Mass (Da):44,289
Last modified:August 22, 2006 - v1
Checksum:iC1DB74AAAE80BA25
GO

Sequence cautioni

The sequence ABF93864.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence ABF93865.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AK065303 differs from that shown. Reason: Frameshift at position 177. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000009 Genomic DNA. Translation: ABF93863.1.
DP000009 Genomic DNA. Translation: ABF93864.1. Sequence problems.
DP000009 Genomic DNA. Translation: ABF93865.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF10813.1.
AK065303 mRNA. No translation available.
RefSeqiNP_001048899.1. NM_001055434.1.
UniGeneiOs.14914.

Genome annotation databases

EnsemblPlantsiOS03T0137200-01; OS03T0137200-01; OS03G0137200.
GeneIDi4331548.
KEGGiosa:4331548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000009 Genomic DNA. Translation: ABF93863.1.
DP000009 Genomic DNA. Translation: ABF93864.1. Sequence problems.
DP000009 Genomic DNA. Translation: ABF93865.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF10813.1.
AK065303 mRNA. No translation available.
RefSeqiNP_001048899.1. NM_001055434.1.
UniGeneiOs.14914.

3D structure databases

ProteinModelPortaliQ10S32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g04430.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0137200-01; OS03T0137200-01; OS03G0137200.
GeneIDi4331548.
KEGGiosa:4331548.

Organism-specific databases

GrameneiQ10S32.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000238703.
InParanoidiQ10S32.
OMAiDSNAMSK.

Gene expression databases

ExpressionAtlasiQ10S32. baseline.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C28_ORYSJ
AccessioniPrimary (citable) accession number: Q10S32
Secondary accession number(s): Q10S30, Q10S31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: August 22, 2006
Last modified: January 7, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.