Q10RB4 (BGAL5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 5
Short name=Lactase 5
EC=3.2.1.23

Gene names

Ordered Locus Names:	Os03g0165400, LOC_Os03g06940
ORF Names:	OJ1123F12.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 841	816	Beta-galactosidase 5		PRO_0000294157
Regions
	Domain	752 – 838	87	SUEL-type lectin
Sites
	Active site	183	1	Proton donor Potential
	Active site	252	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Glycosylation	461	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	475	1	S → R in AAM22973. Ref.1
	Sequence conflict	475	1	S → R in AAM34271. Ref.1
	Sequence conflict	585	1	Q → QFQ in AAM22973. Ref.1
	Sequence conflict	585	1	Q → QFQ in AAM34271. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q10RB4 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 2D35B96E6CB7C250	FASTA	841	92,172
	10 20 30 40 50 60 MGRGCLAALL GGAVAVAVLV AVVHCAVTYD KKAVLVDGQR RILFSGSIHY PRSTPEMWDG 70 80 90 100 110 120 LIEKAKDGGL DVIQTYVFWN GHEPTPGNYN FEGRYDLVRF IKTVQKAGMF VHLRIGPYIC 130 140 150 160 170 180 GEWNFGGFPV WLKYVPGISF RTDNEPFKNA MQGFTEKIVG MMKSENLFAS QGGPIILSQI 190 200 210 220 230 240 ENEYGPEGKE FGAAGKAYIN WAAKMAVGLD TGVPWVMCKE DDAPDPVINA CNGFYCDTFS 250 260 270 280 290 300 PNKPYKPTMW TEAWSGWFTE FGGTIRQRPV EDLAFGVARF VQKGGSFINY YMYHGGTNFG 310 320 330 340 350 360 RTAGGPFITT SYDYDAPLDE YGLAREPKFG HLKELHRAVK LCEQPLVSAD PTVTTLGSMQ 370 380 390 400 410 420 EAHVFRSSSG CAAFLANYNS NSYAKVIFNN ENYSLPPWSI SILPDCKNVV FNTATVGVQT 430 440 450 460 470 480 NQMQMWADGA SSMMWEKYDE EVDSLAAAPL LTSTGLLEQL NVTRDTSDYL WYITSVEVDP 490 500 510 520 530 540 SEKFLQGGTP LSLTVQSAGH ALHVFINGQL QGSAYGTRED RKISYSGNAN LRAGTNKVAL 550 560 570 580 590 600 LSVACGLPNV GVHYETWNTG VVGPVVIHGL DEGSRDLTWQ TWSYQVGLKG EQMNLNSLEG 610 620 630 640 650 660 SGSVEWMQGS LVAQNQQPLA WYRAYFDTPS GDEPLALDMG SMGKGQIWIN GQSIGRYWTA 670 680 690 700 710 720 YAEGDCKGCH YTGSYRAPKC QAGCGQPTQR WYHVPRSWLQ PTRNLLVVFE ELGGDSSKIA 730 740 750 760 770 780 LAKRTVSGVC ADVSEYHPNI KNWQIESYGE PEFHTAKVHL KCAPGQTISA IKFASFGTPL 790 800 810 820 830 840 GTCGTFQQGE CHSINSNSVL EKKCIGLQRC VVAISPSNFG GDPCPEVMKR VAVEAVCSTA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of beta-galactosidase from rice." Chantarangsee M., Mizuno K., Fujimura T., Ketudat-Cairns J.R. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Seed.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF499737 mRNA. Translation: AAM22973.1. AF508799 mRNA. Translation: AAM34271.1. DP000009 Genomic DNA. Translation: ABF94149.1. AP008209 Genomic DNA. Translation: BAF10982.1. AK102192 mRNA. No translation available.
RefSeq	NP_001049068.1. NM_001055603.1.
UniGene	Os.13559.
3D structure databases
ProteinModelPortal	Q10RB4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q10RB4.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q10RB4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g06940.1; LOC_Os03g06940.1; LOC_Os03g06940.
GeneID	4331730.
KEGG	osa:4331730.
Organism-specific databases
Gramene	Q8L3P5.
Phylogenomic databases
GeneTree	EPGT00070000027970.
HOGENOM	HBG318019.
OMA	IGRYWMA.
PhylomeDB	Q10RB4.
ProtClustDB	CLSN2689785.
Enzyme and pathway databases
BRENDA	3.2.1.23. 4460.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL5_ORYSJ

Accession

Primary (citable) accession number: Q10RB4
Secondary accession number(s): Q8L3P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	August 22, 2006
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program