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Q10RB4 (BGAL5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 5

Short name=Lactase 5
EC=3.2.1.23
Gene names
Ordered Locus Names:Os03g0165400, LOC_Os03g06940
ORF Names:OJ1123F12.1
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length841 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 841816Beta-galactosidase 5
PRO_0000294157

Regions

Domain752 – 83887SUEL-type lectin

Sites

Active site1831Proton donor Potential
Active site2521Nucleophile Potential

Amino acid modifications

Glycosylation3921N-linked (GlcNAc...) Potential
Glycosylation4611N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4751S → R in AAM22973. Ref.1
Sequence conflict4751S → R in AAM34271. Ref.1
Sequence conflict5851Q → QFQ in AAM22973. Ref.1
Sequence conflict5851Q → QFQ in AAM34271. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q10RB4 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 2D35B96E6CB7C250

FASTA84192,172
        10         20         30         40         50         60 
MGRGCLAALL GGAVAVAVLV AVVHCAVTYD KKAVLVDGQR RILFSGSIHY PRSTPEMWDG 

        70         80         90        100        110        120 
LIEKAKDGGL DVIQTYVFWN GHEPTPGNYN FEGRYDLVRF IKTVQKAGMF VHLRIGPYIC 

       130        140        150        160        170        180 
GEWNFGGFPV WLKYVPGISF RTDNEPFKNA MQGFTEKIVG MMKSENLFAS QGGPIILSQI 

       190        200        210        220        230        240 
ENEYGPEGKE FGAAGKAYIN WAAKMAVGLD TGVPWVMCKE DDAPDPVINA CNGFYCDTFS 

       250        260        270        280        290        300 
PNKPYKPTMW TEAWSGWFTE FGGTIRQRPV EDLAFGVARF VQKGGSFINY YMYHGGTNFG 

       310        320        330        340        350        360 
RTAGGPFITT SYDYDAPLDE YGLAREPKFG HLKELHRAVK LCEQPLVSAD PTVTTLGSMQ 

       370        380        390        400        410        420 
EAHVFRSSSG CAAFLANYNS NSYAKVIFNN ENYSLPPWSI SILPDCKNVV FNTATVGVQT 

       430        440        450        460        470        480 
NQMQMWADGA SSMMWEKYDE EVDSLAAAPL LTSTGLLEQL NVTRDTSDYL WYITSVEVDP 

       490        500        510        520        530        540 
SEKFLQGGTP LSLTVQSAGH ALHVFINGQL QGSAYGTRED RKISYSGNAN LRAGTNKVAL 

       550        560        570        580        590        600 
LSVACGLPNV GVHYETWNTG VVGPVVIHGL DEGSRDLTWQ TWSYQVGLKG EQMNLNSLEG 

       610        620        630        640        650        660 
SGSVEWMQGS LVAQNQQPLA WYRAYFDTPS GDEPLALDMG SMGKGQIWIN GQSIGRYWTA 

       670        680        690        700        710        720 
YAEGDCKGCH YTGSYRAPKC QAGCGQPTQR WYHVPRSWLQ PTRNLLVVFE ELGGDSSKIA 

       730        740        750        760        770        780 
LAKRTVSGVC ADVSEYHPNI KNWQIESYGE PEFHTAKVHL KCAPGQTISA IKFASFGTPL 

       790        800        810        820        830        840 
GTCGTFQQGE CHSINSNSVL EKKCIGLQRC VVAISPSNFG GDPCPEVMKR VAVEAVCSTA 


A 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of beta-galactosidase from rice."
Chantarangsee M., Mizuno K., Fujimura T., Ketudat-Cairns J.R.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
Tissue: Seed.
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF499737 mRNA. Translation: AAM22973.1.
AF508799 mRNA. Translation: AAM34271.1.
DP000009 Genomic DNA. Translation: ABF94149.1.
AP008209 Genomic DNA. Translation: BAF10982.1.
AK102192 mRNA. No translation available.
RefSeqNP_001049068.1. NM_001055603.1.
UniGeneOs.13559.

3D structure databases

ProteinModelPortalQ10RB4.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PRIDEQ10RB4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0165400-01; OS03T0165400-01; OS03G0165400.
GeneID4331730.
KEGGosa:4331730.

Organism-specific databases

GrameneQ10RB4.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
OMATYDKKAI.

Enzyme and pathway databases

BRENDA3.2.1.23. 4460.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL5_ORYSJ
AccessionPrimary (citable) accession number: Q10RB4
Secondary accession number(s): Q8L3P5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries