Q10QA5 (D14_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable strigolactone esterase D14
EC=3.1.-.-

Alternative name(s):
Protein DWARF-14
Protein DWARF-88
Protein HIGH-TILLERING DWARF 2

Gene names

Name:	D14
Synonyms:	D88, HTD2
Ordered Locus Names:	Os03g0203200, LOC_Os03g10620

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Ref.8 Ref.10
Tissue specificity	Expressed in the parenchyma cells of the root stele and lateral roots, vascular tissues of vein and leaf sheath, ligule base, auricle base and stem base. Ref.1 Ref.8
Disruption phenotype	Increased tillers and reduced plant height, elevated levels of strigolactones. Ref.1 Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the AB hydrolase superfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 318

318

Probable strigolactone esterase D14

PRO_0000422054

Regions

Compositional bias

10 – 53

44

Gly/Ser-rich

Sites

Active site

147

1

Nucleophile Probable

Active site

268

1

By similarity

Active site

297

1

By similarity

Experimental info

Mutagenesis

79

1

G → R in d88; dwarf and high tillering phenotypes. Ref.1

Secondary structure

1

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318

Helix Strand Turn

Details...

Helix

56 – 59

4

Beta strand

63 – 66

4

Beta strand

69 – 75

7

Helix

82 – 85

4

Turn

86 – 88

3

Helix

89 – 91

3

Turn

92 – 95

4

Beta strand

97 – 100

4

Helix

111 – 113

3

Helix

118 – 120

3

Helix

122 – 135

14

Beta strand

141 – 146

6

Helix

148 – 159

12

Turn

161 – 163

3

Beta strand

164 – 171

8

Helix

188 – 200

13

Helix

202 – 214

13

Helix

219 – 230

12

Helix

234 – 245

12

Helix

250 – 255

6

Beta strand

260 – 264

5

Helix

274 – 282

9

Beta strand

287 – 291

5

Helix

299 – 302

4

Helix

304 – 314

11

Sequences

	Sequence		Length	Mass (Da)
	Q10QA5 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 996A6044670563EF	FASTA	318	33,508
	10 20 30 40 50 60 MLRSTHPPPS SPSSSSSGGG GGGGSSASSS SEKTMVGGGG GGGGGSGSAA PSGAKLLQIL 70 80 90 100 110 120 NVRVVGSGER VVVLSHGFGT DQSAWSRVLP YLTRDHRVVL YDLVCAGSVN PDHFDFRRYD 130 140 150 160 170 180 NLDAYVDDLL AILDALRIPR CAFVGHSVSA MIGILASIRR PDLFAKLVLI GASPRFLNDS 190 200 210 220 230 240 DYHGGFELEE IQQVFDAMGA NYSAWATGYA PLAVGADVPA AVQEFSRTLF NMRPDISLHV 250 260 270 280 290 300 CQTVFKTDLR GVLGMVRAPC VVVQTTRDVS VPASVAAYLK AHLGGRTTVE FLQTEGHLPH 310 LSAPSLLAQV LRRALARY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dwarf 88, a novel putative esterase gene affecting architecture of rice plant." Gao Z., Qian Q., Liu X., Yan M., Feng Q., Dong G., Liu J., Han B. Plant Mol. Biol. 71:265-276(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, MUTAGENESIS OF GLY-79, DISRUPTION PHENOTYPE. Strain: cv. Lansheng.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Suppression of tiller bud activity in tillering dwarf mutants of rice." Ishikawa S., Maekawa M., Arite T., Onishi K., Takamure I., Kyozuka J. Plant Cell Physiol. 46:79-86(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: cv. Shiokari.
[7]	"DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice." Arite T., Iwata H., Ohshima K., Maekawa M., Nakajima M., Kojima M., Sakakibara H., Kyozuka J. Plant J. 51:1019-1029(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: cv. Shiokari.
[8]	"d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers." Arite T., Umehara M., Ishikawa S., Hanada A., Maekawa M., Yamaguchi S., Kyozuka J. Plant Cell Physiol. 50:1416-1424(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[9]	"The computational-based structure of Dwarf14 provides evidence for its role as potential strigolactone receptor in plants." Gaiji N., Cardinale F., Prandi C., Bonfante P., Ranghino G. BMC Res. Notes 5:307-307(2012) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[10]	"Structures of D14 and D14L in the strigolactone and karrikin signaling pathways." Kagiyama M., Hirano Y., Mori T., Kim S.Y., Kyozuka J., Seto Y., Yamaguchi S., Hakoshima T. Genes Cells 18:147-160(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 55-318 IN COMPLEX WITH INHIBITOR, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

GQ406388 Genomic DNA. Translation: ACV30015.1.
DP000009 Genomic DNA. Translation: ABF94525.1.
DP000009 Genomic DNA. Translation: ABF94526.1.
AP008209 Genomic DNA. Translation: BAF11220.1.
AK070827 mRNA. Translation: BAG92161.1.

RefSeq

NP_001049306.1. NM_001055841.1.

UniGene

Os.11186.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3W04	X-ray	1.45	A/B	55-318	[»]
3W05	X-ray	1.58	A/B	55-318	[»]
4IH9	X-ray	1.55	A/B	51-318	[»]
4IHA	X-ray	1.55	A/B	51-318	[»]

ProteinModelPortal

Q10QA5.

ModBase

Search...

Protein-protein interaction databases

STRING

39947.LOC_Os03g10620.1.

Proteomic databases

PRIDE

Q10QA5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

LOC_Os03g10620.2; LOC_Os03g10620.2; LOC_Os03g10620.
LOC_Os03g10620.3; LOC_Os03g10620.3; LOC_Os03g10620.

GeneID

4331983.

KEGG

osa:4331983.

Organism-specific databases

Gramene

Q10QA5.

Phylogenomic databases

eggNOG

COG0596.

HOGENOM

HOG000251386.

ProtClustDB

CLSN2684507.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

D14_ORYSJ

Accession

Primary (citable) accession number: Q10QA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2013
Last sequence update:	August 22, 2006
Last modified:	May 29, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program