Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q10QA5 (D14_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable strigolactone esterase D14

EC=3.1.-.-
Alternative name(s):
Protein DWARF-14
Protein DWARF-88
Protein HIGH-TILLERING DWARF 2
Gene names
Name:D14
Synonyms:D88, HTD2
Ordered Locus Names:Os03g0203200, LOC_Os03g10620
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Ref.8 Ref.10

Tissue specificity

Expressed in the parenchyma cells of the root stele and lateral roots, vascular tissues of vein and leaf sheath, ligule base, auricle base and stem base. Ref.1 Ref.8

Disruption phenotype

Increased tillers and reduced plant height, elevated levels of strigolactones. Ref.1 Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the AB hydrolase superfamily.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsecondary shoot formation

Inferred from mutant phenotype Ref.8. Source: UniProtKB

strigolactone biosynthetic process

Inferred from mutant phenotype Ref.8. Source: UniProtKB

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Probable strigolactone esterase D14
PRO_0000422054

Regions

Compositional bias10 – 5344Gly/Ser-rich

Sites

Active site1471Nucleophile Probable
Active site2681 By similarity
Active site2971 By similarity

Experimental info

Mutagenesis791G → R in d88; dwarf and high tillering phenotypes. Ref.1

Secondary structure

............................................... 318
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q10QA5 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 996A6044670563EF

FASTA31833,508
        10         20         30         40         50         60 
MLRSTHPPPS SPSSSSSGGG GGGGSSASSS SEKTMVGGGG GGGGGSGSAA PSGAKLLQIL 

        70         80         90        100        110        120 
NVRVVGSGER VVVLSHGFGT DQSAWSRVLP YLTRDHRVVL YDLVCAGSVN PDHFDFRRYD 

       130        140        150        160        170        180 
NLDAYVDDLL AILDALRIPR CAFVGHSVSA MIGILASIRR PDLFAKLVLI GASPRFLNDS 

       190        200        210        220        230        240 
DYHGGFELEE IQQVFDAMGA NYSAWATGYA PLAVGADVPA AVQEFSRTLF NMRPDISLHV 

       250        260        270        280        290        300 
CQTVFKTDLR GVLGMVRAPC VVVQTTRDVS VPASVAAYLK AHLGGRTTVE FLQTEGHLPH 

       310 
LSAPSLLAQV LRRALARY 

« Hide

References

« Hide 'large scale' references
[1]"Dwarf 88, a novel putative esterase gene affecting architecture of rice plant."
Gao Z., Qian Q., Liu X., Yan M., Feng Q., Dong G., Liu J., Han B.
Plant Mol. Biol. 71:265-276(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, MUTAGENESIS OF GLY-79, DISRUPTION PHENOTYPE.
Strain: cv. Lansheng.
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[6]"Suppression of tiller bud activity in tillering dwarf mutants of rice."
Ishikawa S., Maekawa M., Arite T., Onishi K., Takamure I., Kyozuka J.
Plant Cell Physiol. 46:79-86(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: cv. Shiokari.
[7]"DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice."
Arite T., Iwata H., Ohshima K., Maekawa M., Nakajima M., Kojima M., Sakakibara H., Kyozuka J.
Plant J. 51:1019-1029(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: cv. Shiokari.
[8]"d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers."
Arite T., Umehara M., Ishikawa S., Hanada A., Maekawa M., Yamaguchi S., Kyozuka J.
Plant Cell Physiol. 50:1416-1424(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[9]"The computational-based structure of Dwarf14 provides evidence for its role as potential strigolactone receptor in plants."
Gaiji N., Cardinale F., Prandi C., Bonfante P., Ranghino G.
BMC Res. Notes 5:307-307(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[10]"Structures of D14 and D14L in the strigolactone and karrikin signaling pathways."
Kagiyama M., Hirano Y., Mori T., Kim S.Y., Kyozuka J., Seto Y., Yamaguchi S., Hakoshima T.
Genes Cells 18:147-160(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 55-318 IN COMPLEX WITH INHIBITOR, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GQ406388 Genomic DNA. Translation: ACV30015.1.
DP000009 Genomic DNA. Translation: ABF94525.1.
DP000009 Genomic DNA. Translation: ABF94526.1.
AP008209 Genomic DNA. Translation: BAF11220.1.
AK070827 mRNA. Translation: BAG92161.1.
RefSeqNP_001049306.1. NM_001055841.1.
UniGeneOs.11186.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VXKX-ray1.75A/B54-318[»]
3W04X-ray1.45A/B55-318[»]
3W05X-ray1.58A/B55-318[»]
3WIOX-ray2.10A/B54-318[»]
4IH9X-ray1.55A/B51-318[»]
4IHAX-ray1.55A/B51-318[»]
ProteinModelPortalQ10QA5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os03g10620.1.

Proteomic databases

PRIDEQ10QA5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0203200-01; OS03T0203200-01; OS03G0203200.
GeneID4331983.
KEGGosa:4331983.

Organism-specific databases

GrameneQ10QA5.

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000251386.
ProtClustDBCLSN2684507.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameD14_ORYSJ
AccessionPrimary (citable) accession number: Q10QA5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: August 22, 2006
Last modified: January 22, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PDB cross-references

Index of Protein Data Bank (PDB) cross-references