Reviewed, UniProtKB/Swiss-Prot Q10P67 (DAPB2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable dihydrodipicolinate reductase 2, chloroplastic
      Short name=DHPR 2
    EC=1.3.1.26

Gene names

Name:	DAPB2
Ordered Locus Names:	Os03g0245100, LOC_Os03g14120
ORF Names:	OsJ_009732

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2,3,4,5-tetrahydrodipicolinate + NAD(P)(+) = 2,3-dihydrodipicolinate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 4/4.
Subcellular location	Plastid › chloroplastPotential.
Sequence similarities	Belongs to the dihydrodipicolinate reductase family.
Sequence caution	The sequence EAZ26249.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: InterPro
Molecular function	binding Inferred from electronic annotation. Source: InterPro dihydrodipicolinate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q10P67-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q10P67-2)

The sequence of this isoform differs from the canonical sequence as follows:
     179-203: Missing.

Notes: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Chloroplast Potential
	Chain	33 – 325	293	Probable dihydrodipicolinate reductase 2, chloroplastic		PRO_0000307186
Regions
	Compositional bias	121 – 124	4	Poly-Val
Natural variations
	Alternative sequence	179 – 203	25	Missing in isoform 2.		VSP_028630

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: E1A4D87189A0585F	FASTA	325	35,574
	10 20 30 40 50 60 MLSLRPPCTL SPAPWRRRRT LHGAAGTPQR VSVAAPSAIV EAVSPPARFS FPILVNGCTG 70 80 90 100 110 120 KMGLSVAEAV TSSGLHLVPI SFSSRDTLDR TVRVGHTDVR IYGPSAREDV LSSVIDEFPD 130 140 150 160 170 180 VVVVDYTAPD SVNANAELYC KLGLPFVMGT TGGDRQLLYK SVQDSNNYAL ISPQMGKQVV 190 200 210 220 230 240 AFLAAMEIMA EQFPGAFSGY HLEVLESHQA GKLDISGTAK AVIACFEKLG VSYDMNRMVK 250 260 270 280 290 300 IRDPEQQLEM VGVPEEHIEG HAFHLYHLTS PDDSVSFEFQ HNVCGRSIYA EGSVDAAMFL 310 320 HRKVRSNDSK RIYDMIDVLR EGSMR
	Isoform 2 [UniParc]. Checksum: F3460C7245224459		300	32,862
	10 20 30 40 50 60 MLSLRPPCTL SPAPWRRRRT LHGAAGTPQR VSVAAPSAIV EAVSPPARFS FPILVNGCTG 70 80 90 100 110 120 KMGLSVAEAV TSSGLHLVPI SFSSRDTLDR TVRVGHTDVR IYGPSAREDV LSSVIDEFPD 130 140 150 160 170 180 VVVVDYTAPD SVNANAELYC KLGLPFVMGT TGGDRQLLYK SVQDSNNYAL ISPQMGKQVL 190 200 210 220 230 240 ESHQAGKLDI SGTAKAVIAC FEKLGVSYDM NRMVKIRDPE QQLEMVGVPE EHIEGHAFHL 250 260 270 280 290 300 YHLTSPDDSV SFEFQHNVCG RSIYAEGSVD AAMFLHRKVR SNDSKRIYDM IDVLREGSMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.-I., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DP000009 Genomic DNA. Translation: ABF94935.1. DP000009 Genomic DNA. Translation: ABF94937.1. AP008209 Genomic DNA. Translation: BAF11450.1. Different initiation. CM000140 Genomic DNA. Translation: EAZ26249.1. Sequence problems. AK102253 mRNA. No translation available. AK059346 mRNA. No translation available.
RefSeq	NP_001049536.1.
UniGene	Os.51228
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4332231.
KEGG	osa:4332231.
Family and domain databases
InterPro	IPR000846. DapB. IPR011770. DapB_bac/pln. IPR011859. DapB_pln. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR20836. DapB_bac/pln. 1 hit.
Pfam	PF05173. DapB_C. 1 hit. PF01113. DapB_N. 1 hit. [Graphical view]
ProDom	PD004105. DapB. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02130. dapB_plant. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAPB2_ORYSJ

Accession

Primary (citable) accession number: Q10P67
Secondary accession number(s): A3AG10, Q0DTI8, Q10P66

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	August 22, 2006
Last modified:	November 25, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)