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Protein

Beta-galactosidase 6

Gene

Os03g0255100

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Releases galactose by hydrolysis of plant cell wall galactose-containing polysaccharides such as galacto-xyloglucan, pectic galactan and galactan (in vitro).1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

pH dependencei

Optimum pH is 3.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Proton donorSequence analysis1
Active sitei258NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 6 (EC:3.2.1.23)
Short name:
Lactase 6
Gene namesi
Ordered Locus Names:Os03g0255100, LOC_Os03g15020
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 301 PublicationAdd BLAST30
ChainiPRO_000029415831 – 858Beta-galactosidase 6Add BLAST828

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...)Sequence analysis1
Glycosylationi259N-linked (GlcNAc...)Sequence analysis1
Glycosylationi482N-linked (GlcNAc...)Sequence analysis1
Glycosylationi507N-linked (GlcNAc...)Sequence analysis1
Glycosylationi595N-linked (GlcNAc...)Sequence analysis1
Glycosylationi830N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ10NX8.

Expressioni

Gene expression databases

GenevisibleiQ10NX8. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g15020.1.

Structurei

3D structure databases

ProteinModelPortaliQ10NX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini772 – 858SUEL-type lectinPROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
InParanoidiQ10NX8.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10NX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATVGVLL RLLLLPVVVV VSLLVGASRA ANVTYDHRAV VIDGVRRVLV
60 70 80 90 100
SGSIHYPRST PDMWPGLIQK SKDGGLDVIE TYVFWDIHEA VRGQYDFEGR
110 120 130 140 150
KDLVRFVKAV ADAGLYVHLR IGPYVCAEWN YGGFPVWLHF VPGIKFRTDN
160 170 180 190 200
EAFKAEMQRF TEKVVDTMKG AGLYASQGGP IILSQIENEY GNIDSAYGAA
210 220 230 240 250
GKAYMRWAAG MAVSLDTGVP WVMCQQSDAP DPLINTCNGF YCDQFTPNSK
260 270 280 290 300
SKPKMWTENW SGWFLSFGGA VPYRPAEDLA FAVARFYQRG GTFQNYYMYH
310 320 330 340 350
GGTNFGRSTG GPFIATSYDY DAPIDEYGMV RQPKWGHLRD VHKAIKLCEP
360 370 380 390 400
ALIAAEPSYS SLGQNTEATV YQTADNSICA AFLANVDAQS DKTVKFNGNT
410 420 430 440 450
YKLPAWSVSI LPDCKNVVLN TAQINSQVTT SEMRSLGSSI QDTDDSLITP
460 470 480 490 500
ELATAGWSYA IEPVGITKEN ALTKPGLMEQ INTTADASDF LWYSTSIVVK
510 520 530 540 550
GDEPYLNGSQ SNLLVNSLGH VLQIYINGKL AGSAKGSASS SLISLQTPVT
560 570 580 590 600
LVPGKNKIDL LSTTVGLSNY GAFFDLVGAG VTGPVKLSGP NGALNLSSTD
610 620 630 640 650
WTYQIGLRGE DLHLYNPSEA SPEWVSDNAY PTNQPLIWYK TKFTAPAGDD
660 670 680 690 700
PVAIDFTGMG KGEAWVNGQS IGRYWPTNLA PQSGCVNSCN YRGAYSSNKC
710 720 730 740 750
LKKCGQPSQT LYHVPRSFLQ PGSNDLVLFE QFGGDPSMIS FTTRQTSSIC
760 770 780 790 800
AHVSEMHPAQ IDSWISPQQT SQTQGPALRL ECPREGQVIS NIKFASFGTP
810 820 830 840 850
SGTCGNYNHG ECSSSQALAV VQEACVGMTN CSVPVSSNNF GDPCSGVTKS

LVVEAACS
Length:858
Mass (Da):92,840
Last modified:July 10, 2007 - v2
Checksum:iDBD15B3FB1FF8067
GO

Sequence cautioni

The sequence ABF95027 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAF11505 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79I → F AA sequence (PubMed:12723614).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000009 Genomic DNA. Translation: ABF95027.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF11505.1. Sequence problems.
AP014959 Genomic DNA. No translation available.
RefSeqiXP_015632058.1. XM_015776572.1.
UniGeneiOs.22360.

Genome annotation databases

GeneIDi4332289.
KEGGiosa:4332289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000009 Genomic DNA. Translation: ABF95027.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF11505.1. Sequence problems.
AP014959 Genomic DNA. No translation available.
RefSeqiXP_015632058.1. XM_015776572.1.
UniGeneiOs.22360.

3D structure databases

ProteinModelPortaliQ10NX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g15020.1.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbiQ10NX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4332289.
KEGGiosa:4332289.

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
InParanoidiQ10NX8.

Gene expression databases

GenevisibleiQ10NX8. OS.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL6_ORYSJ
AccessioniPrimary (citable) accession number: Q10NX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 30, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.