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Q10NX8

- BGAL6_ORYSJ

UniProt

Q10NX8 - BGAL6_ORYSJ

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Protein

Beta-galactosidase 6

Gene

Os03g0255100

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Releases galactose by hydrolysis of plant cell wall galactose-containing polysaccharides such as galacto-xyloglucan, pectic galactan and galactan (in vitro).1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

pH dependencei

Optimum pH is 3.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891Proton donorSequence Analysis
Active sitei258 – 2581NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: EnsemblPlants/Gramene

GO - Biological processi

  1. carbohydrate metabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 6 (EC:3.2.1.23)
Short name:
Lactase 6
Gene namesi
Ordered Locus Names:Os03g0255100, LOC_Os03g15020
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10NX8.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 30301 PublicationAdd
BLAST
Chaini31 – 858828Beta-galactosidase 6PRO_0000294158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi595 – 5951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi830 – 8301N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

ExpressionAtlasiQ10NX8. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ10NX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini772 – 85887SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
InParanoidiQ10NX8.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10NX8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAATVGVLL RLLLLPVVVV VSLLVGASRA ANVTYDHRAV VIDGVRRVLV
60 70 80 90 100
SGSIHYPRST PDMWPGLIQK SKDGGLDVIE TYVFWDIHEA VRGQYDFEGR
110 120 130 140 150
KDLVRFVKAV ADAGLYVHLR IGPYVCAEWN YGGFPVWLHF VPGIKFRTDN
160 170 180 190 200
EAFKAEMQRF TEKVVDTMKG AGLYASQGGP IILSQIENEY GNIDSAYGAA
210 220 230 240 250
GKAYMRWAAG MAVSLDTGVP WVMCQQSDAP DPLINTCNGF YCDQFTPNSK
260 270 280 290 300
SKPKMWTENW SGWFLSFGGA VPYRPAEDLA FAVARFYQRG GTFQNYYMYH
310 320 330 340 350
GGTNFGRSTG GPFIATSYDY DAPIDEYGMV RQPKWGHLRD VHKAIKLCEP
360 370 380 390 400
ALIAAEPSYS SLGQNTEATV YQTADNSICA AFLANVDAQS DKTVKFNGNT
410 420 430 440 450
YKLPAWSVSI LPDCKNVVLN TAQINSQVTT SEMRSLGSSI QDTDDSLITP
460 470 480 490 500
ELATAGWSYA IEPVGITKEN ALTKPGLMEQ INTTADASDF LWYSTSIVVK
510 520 530 540 550
GDEPYLNGSQ SNLLVNSLGH VLQIYINGKL AGSAKGSASS SLISLQTPVT
560 570 580 590 600
LVPGKNKIDL LSTTVGLSNY GAFFDLVGAG VTGPVKLSGP NGALNLSSTD
610 620 630 640 650
WTYQIGLRGE DLHLYNPSEA SPEWVSDNAY PTNQPLIWYK TKFTAPAGDD
660 670 680 690 700
PVAIDFTGMG KGEAWVNGQS IGRYWPTNLA PQSGCVNSCN YRGAYSSNKC
710 720 730 740 750
LKKCGQPSQT LYHVPRSFLQ PGSNDLVLFE QFGGDPSMIS FTTRQTSSIC
760 770 780 790 800
AHVSEMHPAQ IDSWISPQQT SQTQGPALRL ECPREGQVIS NIKFASFGTP
810 820 830 840 850
SGTCGNYNHG ECSSSQALAV VQEACVGMTN CSVPVSSNNF GDPCSGVTKS

LVVEAACS
Length:858
Mass (Da):92,840
Last modified:July 10, 2007 - v2
Checksum:iDBD15B3FB1FF8067
GO

Sequence cautioni

The sequence ABF95027.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence BAF11505.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791I → F AA sequence (PubMed:12723614)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000009 Genomic DNA. Translation: ABF95027.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF11505.1. Sequence problems.
RefSeqiNP_001049591.1. NM_001056126.1.
UniGeneiOs.22360.

Genome annotation databases

GeneIDi4332289.
KEGGiosa:4332289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000009 Genomic DNA. Translation: ABF95027.1 . Sequence problems.
AP008209 Genomic DNA. Translation: BAF11505.1 . Sequence problems.
RefSeqi NP_001049591.1. NM_001056126.1.
UniGenei Os.22360.

3D structure databases

ProteinModelPortali Q10NX8.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4332289.
KEGGi osa:4332289.

Organism-specific databases

Gramenei Q10NX8.

Phylogenomic databases

eggNOGi COG1874.
InParanoidi Q10NX8.

Gene expression databases

ExpressionAtlasi Q10NX8. baseline.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Purification and characterization of extracellular beta-galactosidase secreted by supension cultured rice (Oryza sativa L.) cells."
    Kaneko S., Kobayashi H.
    Biosci. Biotechnol. Biochem. 67:627-630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 31-80 AND 453-498, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Nipponbare.
    Tissue: Callus.

Entry informationi

Entry nameiBGAL6_ORYSJ
AccessioniPrimary (citable) accession number: Q10NX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3