Reviewed, UniProtKB/Swiss-Prot Q10NX8 (BGAL6_ORYSJ)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase 6
      Short name=Lactase 6
    EC=3.2.1.23

Gene names

Ordered Locus Names:

Os03g0255100, LOC_Os03g15020

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Releases galactose by hydrolysis of plant cell wall galactose-containing polysaccharides such as galacto-xyloglucan, pectic galactan and galactan (in vitro). Ref.4
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.
Biophysicochemical properties	pH dependence: Optimum pH is 3.5.
Sequence caution	The sequence ABF95027.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF11505.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.4
	Chain	31 – 858	828	Beta-galactosidase 6		PRO_0000294158
Regions
	Domain	772 – 858	87	SUEL-type lectin
Sites
	Active site	189	1	Proton donor Potential
	Active site	258	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	482	1	N-linked (GlcNAc...) Potential
	Glycosylation	507	1	N-linked (GlcNAc...) Potential
	Glycosylation	595	1	N-linked (GlcNAc...) Potential
	Glycosylation	830	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	79	1	I → F AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q10NX8-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: DBD15B3FB1FF8067	FASTA	858	92,840
	10 20 30 40 50 60 MAAATVGVLL RLLLLPVVVV VSLLVGASRA ANVTYDHRAV VIDGVRRVLV SGSIHYPRST 70 80 90 100 110 120 PDMWPGLIQK SKDGGLDVIE TYVFWDIHEA VRGQYDFEGR KDLVRFVKAV ADAGLYVHLR 130 140 150 160 170 180 IGPYVCAEWN YGGFPVWLHF VPGIKFRTDN EAFKAEMQRF TEKVVDTMKG AGLYASQGGP 190 200 210 220 230 240 IILSQIENEY GNIDSAYGAA GKAYMRWAAG MAVSLDTGVP WVMCQQSDAP DPLINTCNGF 250 260 270 280 290 300 YCDQFTPNSK SKPKMWTENW SGWFLSFGGA VPYRPAEDLA FAVARFYQRG GTFQNYYMYH 310 320 330 340 350 360 GGTNFGRSTG GPFIATSYDY DAPIDEYGMV RQPKWGHLRD VHKAIKLCEP ALIAAEPSYS 370 380 390 400 410 420 SLGQNTEATV YQTADNSICA AFLANVDAQS DKTVKFNGNT YKLPAWSVSI LPDCKNVVLN 430 440 450 460 470 480 TAQINSQVTT SEMRSLGSSI QDTDDSLITP ELATAGWSYA IEPVGITKEN ALTKPGLMEQ 490 500 510 520 530 540 INTTADASDF LWYSTSIVVK GDEPYLNGSQ SNLLVNSLGH VLQIYINGKL AGSAKGSASS 550 560 570 580 590 600 SLISLQTPVT LVPGKNKIDL LSTTVGLSNY GAFFDLVGAG VTGPVKLSGP NGALNLSSTD 610 620 630 640 650 660 WTYQIGLRGE DLHLYNPSEA SPEWVSDNAY PTNQPLIWYK TKFTAPAGDD PVAIDFTGMG 670 680 690 700 710 720 KGEAWVNGQS IGRYWPTNLA PQSGCVNSCN YRGAYSSNKC LKKCGQPSQT LYHVPRSFLQ 730 740 750 760 770 780 PGSNDLVLFE QFGGDPSMIS FTTRQTSSIC AHVSEMHPAQ IDSWISPQQT SQTQGPALRL 790 800 810 820 830 840 ECPREGQVIS NIKFASFGTP SGTCGNYNHG ECSSSQALAV VQEACVGMTN CSVPVSSNNF 850 GDPCSGVTKS LVVEAACS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Purification and characterization of extracellular beta-galactosidase secreted by supension cultured rice (Oryza sativa L.) cells." Kaneko S., Kobayashi H. Biosci. Biotechnol. Biochem. 67:627-630(2003) [PubMed: 12723614] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-80 AND 453-498, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Nipponbare. Tissue: Callus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DP000009 Genomic DNA. Translation: ABF95027.1. Sequence problems. AP008209 Genomic DNA. Translation: BAF11505.1. Sequence problems.
RefSeq	NP_001049591.1.
UniGene	Os.22360
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Genome annotation databases
GeneID	4332289.
KEGG	osa:4332289.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR000922. Lectin_gal_bd. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
ProDom	PD005612. Gal_lectin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL6_ORYSJ

Accession

Primary (citable) accession number: Q10NX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	September 22, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)