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Q10NX8 (BGAL6_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 6

Short name=Lactase 6
EC=3.2.1.23
Gene names
Ordered Locus Names:Os03g0255100, LOC_Os03g15020
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Releases galactose by hydrolysis of plant cell wall galactose-containing polysaccharides such as galacto-xyloglucan, pectic galactan and galactan (in vitro). Ref.4

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Probable.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 3.5. Ref.4

Sequence caution

The sequence ABF95027.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAF11505.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Ref.4
Chain31 – 858828Beta-galactosidase 6
PRO_0000294158

Regions

Domain772 – 85887SUEL-type lectin

Sites

Active site1891Proton donor Potential
Active site2581Nucleophile Potential

Amino acid modifications

Glycosylation321N-linked (GlcNAc...) Potential
Glycosylation2591N-linked (GlcNAc...) Potential
Glycosylation4821N-linked (GlcNAc...) Potential
Glycosylation5071N-linked (GlcNAc...) Potential
Glycosylation5951N-linked (GlcNAc...) Potential
Glycosylation8301N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict791I → F AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q10NX8 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: DBD15B3FB1FF8067

FASTA85892,840
        10         20         30         40         50         60 
MAAATVGVLL RLLLLPVVVV VSLLVGASRA ANVTYDHRAV VIDGVRRVLV SGSIHYPRST 

        70         80         90        100        110        120 
PDMWPGLIQK SKDGGLDVIE TYVFWDIHEA VRGQYDFEGR KDLVRFVKAV ADAGLYVHLR 

       130        140        150        160        170        180 
IGPYVCAEWN YGGFPVWLHF VPGIKFRTDN EAFKAEMQRF TEKVVDTMKG AGLYASQGGP 

       190        200        210        220        230        240 
IILSQIENEY GNIDSAYGAA GKAYMRWAAG MAVSLDTGVP WVMCQQSDAP DPLINTCNGF 

       250        260        270        280        290        300 
YCDQFTPNSK SKPKMWTENW SGWFLSFGGA VPYRPAEDLA FAVARFYQRG GTFQNYYMYH 

       310        320        330        340        350        360 
GGTNFGRSTG GPFIATSYDY DAPIDEYGMV RQPKWGHLRD VHKAIKLCEP ALIAAEPSYS 

       370        380        390        400        410        420 
SLGQNTEATV YQTADNSICA AFLANVDAQS DKTVKFNGNT YKLPAWSVSI LPDCKNVVLN 

       430        440        450        460        470        480 
TAQINSQVTT SEMRSLGSSI QDTDDSLITP ELATAGWSYA IEPVGITKEN ALTKPGLMEQ 

       490        500        510        520        530        540 
INTTADASDF LWYSTSIVVK GDEPYLNGSQ SNLLVNSLGH VLQIYINGKL AGSAKGSASS 

       550        560        570        580        590        600 
SLISLQTPVT LVPGKNKIDL LSTTVGLSNY GAFFDLVGAG VTGPVKLSGP NGALNLSSTD 

       610        620        630        640        650        660 
WTYQIGLRGE DLHLYNPSEA SPEWVSDNAY PTNQPLIWYK TKFTAPAGDD PVAIDFTGMG 

       670        680        690        700        710        720 
KGEAWVNGQS IGRYWPTNLA PQSGCVNSCN YRGAYSSNKC LKKCGQPSQT LYHVPRSFLQ 

       730        740        750        760        770        780 
PGSNDLVLFE QFGGDPSMIS FTTRQTSSIC AHVSEMHPAQ IDSWISPQQT SQTQGPALRL 

       790        800        810        820        830        840 
ECPREGQVIS NIKFASFGTP SGTCGNYNHG ECSSSQALAV VQEACVGMTN CSVPVSSNNF 

       850 
GDPCSGVTKS LVVEAACS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Purification and characterization of extracellular beta-galactosidase secreted by supension cultured rice (Oryza sativa L.) cells."
Kaneko S., Kobayashi H.
Biosci. Biotechnol. Biochem. 67:627-630(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 31-80 AND 453-498, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: cv. Nipponbare.
Tissue: Callus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DP000009 Genomic DNA. Translation: ABF95027.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF11505.1. Sequence problems.
RefSeqNP_001049591.1. NM_001056126.1.
UniGeneOs.22360.

3D structure databases

ProteinModelPortalQ10NX8.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4332289.
KEGGosa:4332289.

Organism-specific databases

GrameneQ10NX8.

Phylogenomic databases

eggNOGCOG1874.
ProtClustDBCLSN2690970.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL6_ORYSJ
AccessionPrimary (citable) accession number: Q10NX8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries