Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q10NX8

- BGAL6_ORYSJ

UniProt

Q10NX8 - BGAL6_ORYSJ

Protein

Beta-galactosidase 6

Gene

Os03g0255100

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Releases galactose by hydrolysis of plant cell wall galactose-containing polysaccharides such as galacto-xyloglucan, pectic galactan and galactan (in vitro).1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    pH dependencei

    Optimum pH is 3.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei189 – 1891Proton donorSequence Analysis
    Active sitei258 – 2581NucleophileSequence Analysis

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase 6 (EC:3.2.1.23)
    Short name:
    Lactase 6
    Gene namesi
    Ordered Locus Names:Os03g0255100, LOC_Os03g15020
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ10NX8.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 30301 PublicationAdd
    BLAST
    Chaini31 – 858828Beta-galactosidase 6PRO_0000294158Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi595 – 5951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi830 – 8301N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ10NX8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini772 – 85887SUEL-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated
    Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    IPR000922. Lectin_gal-bd_dom.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF02140. Gal_Lectin. 1 hit.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q10NX8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAATVGVLL RLLLLPVVVV VSLLVGASRA ANVTYDHRAV VIDGVRRVLV    50
    SGSIHYPRST PDMWPGLIQK SKDGGLDVIE TYVFWDIHEA VRGQYDFEGR 100
    KDLVRFVKAV ADAGLYVHLR IGPYVCAEWN YGGFPVWLHF VPGIKFRTDN 150
    EAFKAEMQRF TEKVVDTMKG AGLYASQGGP IILSQIENEY GNIDSAYGAA 200
    GKAYMRWAAG MAVSLDTGVP WVMCQQSDAP DPLINTCNGF YCDQFTPNSK 250
    SKPKMWTENW SGWFLSFGGA VPYRPAEDLA FAVARFYQRG GTFQNYYMYH 300
    GGTNFGRSTG GPFIATSYDY DAPIDEYGMV RQPKWGHLRD VHKAIKLCEP 350
    ALIAAEPSYS SLGQNTEATV YQTADNSICA AFLANVDAQS DKTVKFNGNT 400
    YKLPAWSVSI LPDCKNVVLN TAQINSQVTT SEMRSLGSSI QDTDDSLITP 450
    ELATAGWSYA IEPVGITKEN ALTKPGLMEQ INTTADASDF LWYSTSIVVK 500
    GDEPYLNGSQ SNLLVNSLGH VLQIYINGKL AGSAKGSASS SLISLQTPVT 550
    LVPGKNKIDL LSTTVGLSNY GAFFDLVGAG VTGPVKLSGP NGALNLSSTD 600
    WTYQIGLRGE DLHLYNPSEA SPEWVSDNAY PTNQPLIWYK TKFTAPAGDD 650
    PVAIDFTGMG KGEAWVNGQS IGRYWPTNLA PQSGCVNSCN YRGAYSSNKC 700
    LKKCGQPSQT LYHVPRSFLQ PGSNDLVLFE QFGGDPSMIS FTTRQTSSIC 750
    AHVSEMHPAQ IDSWISPQQT SQTQGPALRL ECPREGQVIS NIKFASFGTP 800
    SGTCGNYNHG ECSSSQALAV VQEACVGMTN CSVPVSSNNF GDPCSGVTKS 850
    LVVEAACS 858
    Length:858
    Mass (Da):92,840
    Last modified:July 10, 2007 - v2
    Checksum:iDBD15B3FB1FF8067
    GO

    Sequence cautioni

    The sequence ABF95027.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence BAF11505.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti79 – 791I → F AA sequence (PubMed:12723614)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DP000009 Genomic DNA. Translation: ABF95027.1. Sequence problems.
    AP008209 Genomic DNA. Translation: BAF11505.1. Sequence problems.
    RefSeqiNP_001049591.1. NM_001056126.1.
    UniGeneiOs.22360.

    Genome annotation databases

    GeneIDi4332289.
    KEGGiosa:4332289.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DP000009 Genomic DNA. Translation: ABF95027.1 . Sequence problems.
    AP008209 Genomic DNA. Translation: BAF11505.1 . Sequence problems.
    RefSeqi NP_001049591.1. NM_001056126.1.
    UniGenei Os.22360.

    3D structure databases

    ProteinModelPortali Q10NX8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4332289.
    KEGGi osa:4332289.

    Organism-specific databases

    Gramenei Q10NX8.

    Phylogenomic databases

    eggNOGi COG1874.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    IPR000922. Lectin_gal-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF02140. Gal_Lectin. 1 hit.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "Purification and characterization of extracellular beta-galactosidase secreted by supension cultured rice (Oryza sativa L.) cells."
      Kaneko S., Kobayashi H.
      Biosci. Biotechnol. Biochem. 67:627-630(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 31-80 AND 453-498, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Nipponbare.
      Tissue: Callus.

    Entry informationi

    Entry nameiBGAL6_ORYSJ
    AccessioniPrimary (citable) accession number: Q10NX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3