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Q10N20

- MPK5_ORYSJ

UniProt

Q10N20 - MPK5_ORYSJ

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Protein

Mitogen-activated protein kinase 5

Gene

MPK5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated in response to low temperature (12 degrees Celsius) treatment.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651ATPPROSITE-ProRule annotation
Active sitei162 – 1621Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  2. activation of MAPK activity involved in osmosensory signaling pathway Source: EnsemblPlants/Gramene
  3. camalexin biosynthetic process Source: EnsemblPlants/Gramene
  4. defense response to bacterium Source: EnsemblPlants/Gramene
  5. defense response to fungus Source: EnsemblPlants/Gramene
  6. detection of biotic stimulus Source: EnsemblPlants/Gramene
  7. inflorescence development Source: EnsemblPlants/Gramene
  8. jasmonic acid mediated signaling pathway Source: EnsemblPlants/Gramene
  9. negative regulation of defense response Source: EnsemblPlants/Gramene
  10. negative regulation of programmed cell death Source: EnsemblPlants/Gramene
  11. ovule development Source: EnsemblPlants/Gramene
  12. photosynthesis, light reaction Source: EnsemblPlants/Gramene
  13. plant-type hypersensitive response Source: EnsemblPlants/Gramene
  14. priming of cellular response to stress Source: EnsemblPlants/Gramene
  15. protein targeting to membrane Source: EnsemblPlants/Gramene
  16. regulation of hydrogen peroxide metabolic process Source: EnsemblPlants/Gramene
  17. regulation of multi-organism process Source: EnsemblPlants/Gramene
  18. regulation of plant-type hypersensitive response Source: EnsemblPlants/Gramene
  19. regulation of protein dephosphorylation Source: EnsemblPlants/Gramene
  20. regulation of stomatal complex development Source: EnsemblPlants/Gramene
  21. regulation of stomatal complex patterning Source: EnsemblPlants/Gramene
  22. regulation of transcription, DNA-templated Source: EnsemblPlants/Gramene
  23. response to cold Source: EnsemblPlants/Gramene
  24. response to hypoxia Source: EnsemblPlants/Gramene
  25. response to osmotic stress Source: EnsemblPlants/Gramene
  26. response to UV-B Source: EnsemblPlants/Gramene
  27. salicylic acid biosynthetic process Source: EnsemblPlants/Gramene
  28. stomatal complex development Source: EnsemblPlants/Gramene
  29. systemic acquired resistance, salicylic acid mediated signaling pathway Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203586. ERK/MAPK targets.
REACT_215626. ERK2 activation.
REACT_221522. ERKs are inactivated.
REACT_221537. ERK1 activation.
REACT_224658. Regulation of HSF1-mediated heat shock response.
REACT_233484. Signal transduction by L1.
REACT_248492. ISG15 antiviral mechanism.
REACT_261734. Signaling by FGFR.
REACT_268490. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 5 (EC:2.7.11.24)
Short name:
MAP kinase 5
Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2
Gene namesi
Name:MPK5
Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
Ordered Locus Names:Os03g0285800, LOC_Os03g17700
ORF Names:OSJNBa0013D02.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10N20.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: EnsemblPlants/Gramene
  2. nucleus Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Mitogen-activated protein kinase 5PRO_0000239748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941PhosphothreonineBy similarity
Modified residuei196 – 1961PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-194 and Tyr-196, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in roots, stems and panicles, and at lower levels in leaves.1 Publication

Inductioni

By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors.5 Publications

Gene expression databases

ExpressionAtlasiQ10N20. baseline and differential.

Interactioni

Subunit structurei

Interacts with MKK1.1 Publication

Protein-protein interaction databases

IntActiQ10N20. 2 interactions.
MINTiMINT-7234365.

Structurei

3D structure databases

ProteinModelPortaliQ10N20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 322287Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi194 – 1963TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiQ10N20.
KOiK04371.
OMAiIADEPIC.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform MAPK5a (identifier: Q10N20-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV
60 70 80 90 100
CSVMNFETRE MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD
110 120 130 140 150
VIPPPIPQAF NDVYIATELM DTDLHHIIRS NQELSEEHCQ YFLYQILRGL
160 170 180 190 200
KYIHSANVIH RDLKPSNLLL NANCDLKICD FGLARPSSES DMMTEYVVTR
210 220 230 240 250
WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR DHMHQMRLIT
260 270 280 290 300
EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE
310 320 330 340 350
RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED
360
QMKQLIFNEA IEMNPNIRY
Length:369
Mass (Da):42,995
Last modified:August 22, 2006 - v1
Checksum:i417D81732635F2D3
GO
Isoform MAPK5b (identifier: Q10N20-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-173: Missing.

Note: Does not possess kinase activity.

Show »
Length:265
Mass (Da):30,817
Checksum:i65AB271A79D98F8C
GO

Sequence cautioni

The sequence AAO16999.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461A → G in CAC13967. (PubMed:11975731)Curated
Sequence conflicti89 – 891H → L in CAC13967. (PubMed:11975731)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei70 – 173104Missing in isoform MAPK5b. 1 PublicationVSP_019261Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ486975 mRNA. Translation: CAD31224.1.
AF216315 mRNA. Translation: AAG40579.1.
AJ250311 mRNA. Translation: CAC13967.1.
AF479883 mRNA. Translation: AAL87689.1.
AF479884 mRNA. Translation: AAL87690.1.
AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1.
AP008209 Genomic DNA. Translation: BAF11684.1.
RefSeqiNP_001049770.1. NM_001056305.1. [Q10N20-1]
UniGeneiOs.406.

Genome annotation databases

EnsemblPlantsiOS03T0285800-01; OS03T0285800-01; OS03G0285800. [Q10N20-1]
OS03T0285800-02; OS03T0285800-02; OS03G0285800. [Q10N20-1]
GeneIDi4332475.
KEGGiosa:4332475.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ486975 mRNA. Translation: CAD31224.1 .
AF216315 mRNA. Translation: AAG40579.1 .
AJ250311 mRNA. Translation: CAC13967.1 .
AF479883 mRNA. Translation: AAL87689.1 .
AF479884 mRNA. Translation: AAL87690.1 .
AC134232 Genomic DNA. Translation: AAO16999.1 . Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1 .
AP008209 Genomic DNA. Translation: BAF11684.1 .
RefSeqi NP_001049770.1. NM_001056305.1. [Q10N20-1 ]
UniGenei Os.406.

3D structure databases

ProteinModelPortali Q10N20.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q10N20. 2 interactions.
MINTi MINT-7234365.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0285800-01 ; OS03T0285800-01 ; OS03G0285800 . [Q10N20-1 ]
OS03T0285800-02 ; OS03T0285800-02 ; OS03G0285800 . [Q10N20-1 ]
GeneIDi 4332475.
KEGGi osa:4332475.

Organism-specific databases

Gramenei Q10N20.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi Q10N20.
KOi K04371.
OMAi IADEPIC.

Enzyme and pathway databases

Reactomei REACT_203586. ERK/MAPK targets.
REACT_215626. ERK2 activation.
REACT_221522. ERKs are inactivated.
REACT_221537. ERK1 activation.
REACT_224658. Regulation of HSF1-mediated heat shock response.
REACT_233484. Signal transduction by L1.
REACT_248492. ISG15 antiviral mechanism.
REACT_261734. Signaling by FGFR.
REACT_268490. Signalling to ERK5.

Gene expression databases

ExpressionAtlasi Q10N20. baseline and differential.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues."
    Agrawal G.K., Rakwal R., Iwahashi H.
    Biochem. Biophys. Res. Commun. 294:1009-1016(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), INDUCTION.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  2. "Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
    Wen J.-Q., Oono K., Imai R.
    Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), ENZYME REGULATION, INTERACTION WITH MKK1, INDUCTION.
  3. "Expression of Oryza sativa MAP kinase gene is developmentally regulated and stress-responsive."
    Huang H.-J., Fu S.-F., Tai Y.-H., Chou W.-C., Huang D.-D.
    Physiol. Plantarum 114:572-580(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), TISSUE SPECIFICITY, INDUCTION, PHOSPHORYLATION.
  4. "Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase."
    Xiong L., Yang Y.
    Plant Cell 15:745-759(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MAPK5A AND MAPK5B), FUNCTION, ENZYME REGULATION, INDUCTION, ALTERNATIVE SPLICING.
  5. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  7. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  8. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK5_ORYSJ
AccessioniPrimary (citable) accession number: Q10N20
Secondary accession number(s): Q7FNE2
, Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 22, 2006
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3