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Protein

Mitogen-activated protein kinase 5

Gene

MPK5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance (PubMed:12615946). Functions downstream of CPK18 in a signaling pathway that represses defense gene expression and negatively regulates resistance to rice blast fungus. Phosphorylated by CPK18 at Thr-14 and Thr-32 and activated independently of MAP kinase kinase (MKK) phosphorylation (PubMed:25035404).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated in response to low temperature (12 degrees Celsius) treatment (PubMed:12177502, PubMed:12615946). Activated by phosphorylation at Thr-14 and Thr-32 by CPK18 (PubMed:25035404).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATPPROSITE-ProRule annotation1
Active sitei162Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 5 (EC:2.7.11.24)
Short name:
MAP kinase 5
Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2
Gene namesi
Name:MPK5
Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
Ordered Locus Names:Os03g0285800, LOC_Os03g17700
ORF Names:OsJ_10412Imported, OSJNBa0013D02.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14T → A: Loss of phosphorylation. 1 Publication1
Mutagenesisi32T → A: Loss of phosphorylation. 1 Publication1
Mutagenesisi65K → R: Loss of autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397481 – 369Mitogen-activated protein kinase 5Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14Phosphothreonine1 Publication1
Modified residuei32Phosphothreonine1 Publication1
Modified residuei194PhosphothreonineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated at Thr-194 and Tyr-196, which activates the enzyme (By similarity). Autophosphorylated. Phosphorylated by CPK18 at Thr-14 and Thr-32, which activates the enzyme (PubMed:25035404).By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ10N20.

Expressioni

Tissue specificityi

Expressed in roots, stems and panicles, and at lower levels in leaves.1 Publication

Inductioni

By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors.5 Publications

Gene expression databases

GenevisibleiQ10N20. OS.

Interactioni

Subunit structurei

Interacts with MKK1 (PubMed:12177502). Interacts with CPK18 (PubMed:25035404).2 Publications

Protein-protein interaction databases

IntActiQ10N20. 2 interactors.
MINTiMINT-7234365.
STRINGi39947.LOC_Os03g17700.1.

Structurei

3D structure databases

ProteinModelPortaliQ10N20.
SMRiQ10N20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 196TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
InParanoidiQ10N20.
KOiK04371.
OMAiYIGTELM.
OrthoDBiEOG09360BWL.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform MAPK5a (identifier: Q10N20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV
60 70 80 90 100
CSVMNFETRE MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD
110 120 130 140 150
VIPPPIPQAF NDVYIATELM DTDLHHIIRS NQELSEEHCQ YFLYQILRGL
160 170 180 190 200
KYIHSANVIH RDLKPSNLLL NANCDLKICD FGLARPSSES DMMTEYVVTR
210 220 230 240 250
WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR DHMHQMRLIT
260 270 280 290 300
EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE
310 320 330 340 350
RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED
360
QMKQLIFNEA IEMNPNIRY
Length:369
Mass (Da):42,995
Last modified:August 22, 2006 - v1
Checksum:i417D81732635F2D3
GO
Isoform MAPK5b (identifier: Q10N20-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-173: Missing.

Note: Does not possess kinase activity.
Show »
Length:265
Mass (Da):30,817
Checksum:i65AB271A79D98F8C
GO

Sequence cautioni

The sequence AAO16999 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46A → G in CAC13967 (PubMed:11975731).Curated1
Sequence conflicti89H → L in CAC13967 (PubMed:11975731).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01926170 – 173Missing in isoform MAPK5b. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ486975 mRNA. Translation: CAD31224.1.
AF216315 mRNA. Translation: AAG40579.1.
AJ250311 mRNA. Translation: CAC13967.1.
AF479883 mRNA. Translation: AAL87689.1.
AF479884 mRNA. Translation: AAL87690.1.
AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1.
AP008209 Genomic DNA. Translation: BAF11684.1.
AP014959 Genomic DNA. Translation: BAS83624.1.
CM000140 Genomic DNA. Translation: EEE58837.1.
AK067339 mRNA. Translation: BAG90374.1.
AK104834 mRNA. Translation: BAG96983.1.
RefSeqiXP_015630496.1. XM_015775010.1. [Q10N20-1]
UniGeneiOs.406.

Genome annotation databases

EnsemblPlantsiOS03T0285800-01; OS03T0285800-01; OS03G0285800. [Q10N20-1]
OS03T0285800-02; OS03T0285800-02; OS03G0285800. [Q10N20-1]
GeneIDi4332475.
GrameneiOS03T0285800-01; OS03T0285800-01; OS03G0285800.
OS03T0285800-02; OS03T0285800-02; OS03G0285800.
KEGGiosa:4332475.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ486975 mRNA. Translation: CAD31224.1.
AF216315 mRNA. Translation: AAG40579.1.
AJ250311 mRNA. Translation: CAC13967.1.
AF479883 mRNA. Translation: AAL87689.1.
AF479884 mRNA. Translation: AAL87690.1.
AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1.
AP008209 Genomic DNA. Translation: BAF11684.1.
AP014959 Genomic DNA. Translation: BAS83624.1.
CM000140 Genomic DNA. Translation: EEE58837.1.
AK067339 mRNA. Translation: BAG90374.1.
AK104834 mRNA. Translation: BAG96983.1.
RefSeqiXP_015630496.1. XM_015775010.1. [Q10N20-1]
UniGeneiOs.406.

3D structure databases

ProteinModelPortaliQ10N20.
SMRiQ10N20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ10N20. 2 interactors.
MINTiMINT-7234365.
STRINGi39947.LOC_Os03g17700.1.

Proteomic databases

PaxDbiQ10N20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0285800-01; OS03T0285800-01; OS03G0285800. [Q10N20-1]
OS03T0285800-02; OS03T0285800-02; OS03G0285800. [Q10N20-1]
GeneIDi4332475.
GrameneiOS03T0285800-01; OS03T0285800-01; OS03G0285800.
OS03T0285800-02; OS03T0285800-02; OS03G0285800.
KEGGiosa:4332475.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
InParanoidiQ10N20.
KOiK04371.
OMAiYIGTELM.
OrthoDBiEOG09360BWL.

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Gene expression databases

GenevisibleiQ10N20. OS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK5_ORYSJ
AccessioniPrimary (citable) accession number: Q10N20
Secondary accession number(s): B7EDC7
, Q7FNE2, Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 22, 2006
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.