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Q10N20

- MPK5_ORYSJ

UniProt

Q10N20 - MPK5_ORYSJ

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Protein
Mitogen-activated protein kinase 5
Gene
MPK5, BIMK1, MAPK2, MAPK5, MPK3, MSRMK2, Os03g0285800, LOC_Os03g17700, OSJNBa0013D02.9
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to low temperature (12 degrees Celsius) treatment.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651ATP By similarity
Active sitei162 – 1621Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. activation of MAPK activity involved in osmosensory signaling pathway Source: EnsemblPlants/Gramene
  2. camalexin biosynthetic process Source: EnsemblPlants/Gramene
  3. defense response Source: UniProtKB-KW
  4. inflorescence development Source: EnsemblPlants/Gramene
  5. ovule development Source: EnsemblPlants/Gramene
  6. priming of cellular response to stress Source: EnsemblPlants/Gramene
  7. regulation of stomatal complex development Source: EnsemblPlants/Gramene
  8. regulation of stomatal complex patterning Source: EnsemblPlants/Gramene
  9. response to UV-B Source: EnsemblPlants/Gramene
  10. response to bacterium Source: EnsemblPlants/Gramene
  11. response to chitin Source: EnsemblPlants/Gramene
  12. response to osmotic stress Source: EnsemblPlants/Gramene
  13. response to oxidative stress Source: EnsemblPlants/Gramene
  14. response to wounding Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_205014. CREB phosphorylation through the activation of Ras.
REACT_214549. Signalling to ERK5.
REACT_215626. ERK2 activation.
REACT_221522. ERKs are inactivated.
REACT_221537. ERK1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 5 (EC:2.7.11.24)
Short name:
MAP kinase 5
Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2
Gene namesi
Name:MPK5
Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
Ordered Locus Names:Os03g0285800, LOC_Os03g17700
ORF Names:OSJNBa0013D02.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10N20.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Mitogen-activated protein kinase 5
PRO_0000239748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941Phosphothreonine By similarity
Modified residuei196 – 1961Phosphotyrosine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-194 and Tyr-196, which activates the enzyme By similarity.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in roots, stems and panicles, and at lower levels in leaves.1 Publication

Inductioni

By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors.5 Publications

Interactioni

Subunit structurei

Interacts with MKK1.1 Publication

Protein-protein interaction databases

IntActiQ10N20. 2 interactions.
MINTiMINT-7234365.

Structurei

3D structure databases

ProteinModelPortaliQ10N20.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 322287Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi194 – 1963TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
KOiK04371.
OMAiIADEPIC.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform MAPK5a (identifier: Q10N20-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV    50
CSVMNFETRE MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD 100
VIPPPIPQAF NDVYIATELM DTDLHHIIRS NQELSEEHCQ YFLYQILRGL 150
KYIHSANVIH RDLKPSNLLL NANCDLKICD FGLARPSSES DMMTEYVVTR 200
WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR DHMHQMRLIT 250
EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE 300
RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED 350
QMKQLIFNEA IEMNPNIRY 369
Length:369
Mass (Da):42,995
Last modified:August 22, 2006 - v1
Checksum:i417D81732635F2D3
GO
Isoform MAPK5b (identifier: Q10N20-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-173: Missing.

Note: Does not possess kinase activity.

Show »
Length:265
Mass (Da):30,817
Checksum:i65AB271A79D98F8C
GO

Sequence cautioni

The sequence AAO16999.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei70 – 173104Missing in isoform MAPK5b.
VSP_019261Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461A → G in CAC13967. 1 Publication
Sequence conflicti89 – 891H → L in CAC13967. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ486975 mRNA. Translation: CAD31224.1.
AF216315 mRNA. Translation: AAG40579.1.
AJ250311 mRNA. Translation: CAC13967.1.
AF479883 mRNA. Translation: AAL87689.1.
AF479884 mRNA. Translation: AAL87690.1.
AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1.
AP008209 Genomic DNA. Translation: BAF11684.1.
RefSeqiNP_001049770.1. NM_001056305.1. [Q10N20-1]
UniGeneiOs.406.

Genome annotation databases

EnsemblPlantsiOS03T0285800-01; OS03T0285800-01; OS03G0285800. [Q10N20-1]
OS03T0285800-02; OS03T0285800-02; OS03G0285800. [Q10N20-1]
GeneIDi4332475.
KEGGiosa:4332475.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ486975 mRNA. Translation: CAD31224.1 .
AF216315 mRNA. Translation: AAG40579.1 .
AJ250311 mRNA. Translation: CAC13967.1 .
AF479883 mRNA. Translation: AAL87689.1 .
AF479884 mRNA. Translation: AAL87690.1 .
AC134232 Genomic DNA. Translation: AAO16999.1 . Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1 .
AP008209 Genomic DNA. Translation: BAF11684.1 .
RefSeqi NP_001049770.1. NM_001056305.1. [Q10N20-1 ]
UniGenei Os.406.

3D structure databases

ProteinModelPortali Q10N20.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q10N20. 2 interactions.
MINTi MINT-7234365.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0285800-01 ; OS03T0285800-01 ; OS03G0285800 . [Q10N20-1 ]
OS03T0285800-02 ; OS03T0285800-02 ; OS03G0285800 . [Q10N20-1 ]
GeneIDi 4332475.
KEGGi osa:4332475.

Organism-specific databases

Gramenei Q10N20.

Phylogenomic databases

eggNOGi COG0515.
KOi K04371.
OMAi IADEPIC.

Enzyme and pathway databases

Reactomei REACT_205014. CREB phosphorylation through the activation of Ras.
REACT_214549. Signalling to ERK5.
REACT_215626. ERK2 activation.
REACT_221522. ERKs are inactivated.
REACT_221537. ERK1 activation.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues."
    Agrawal G.K., Rakwal R., Iwahashi H.
    Biochem. Biophys. Res. Commun. 294:1009-1016(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), INDUCTION.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  2. "Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
    Wen J.-Q., Oono K., Imai R.
    Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), ENZYME REGULATION, INTERACTION WITH MKK1, INDUCTION.
  3. "Expression of Oryza sativa MAP kinase gene is developmentally regulated and stress-responsive."
    Huang H.-J., Fu S.-F., Tai Y.-H., Chou W.-C., Huang D.-D.
    Physiol. Plantarum 114:572-580(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), TISSUE SPECIFICITY, INDUCTION, PHOSPHORYLATION.
  4. "Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase."
    Xiong L., Yang Y.
    Plant Cell 15:745-759(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MAPK5A AND MAPK5B), FUNCTION, ENZYME REGULATION, INDUCTION, ALTERNATIVE SPLICING.
  5. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  7. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  8. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK5_ORYSJ
AccessioniPrimary (citable) accession number: Q10N20
Secondary accession number(s): Q7FNE2
, Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 22, 2006
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi