Q10N20 (MPK5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase 5
Short name=MAP kinase 5
EC=2.7.11.24

Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2

Gene names

Name:	MPK5
Synonyms:	BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
Ordered Locus Names:	Os03g0285800, LOC_Os03g17700
ORF Names:	OSJNBa0013D02.9

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance. Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to low temperature (12 degrees Celsius) treatment. Ref.2 Ref.4
Subunit structure	Interacts with MEK1. Ref.2
Tissue specificity	Expressed in roots, stems and panicles, and at lower levels in leaves. Ref.3
Induction	By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors. Ref.1 Ref.2 Ref.3 Ref.4 Ref.8
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-194 and Tyr-196, which activates the enzyme By similarity. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAO16999.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Plant defense
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: GOC activation of MAPK activity involved in osmosensory signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene camalexin biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene defense response Inferred from electronic annotation. Source: UniProtKB-KW ovule development Inferred from electronic annotation. Source: EnsemblPlants/Gramene priming of cellular response to stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of stomatal complex development Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of stomatal complex patterning Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to bacterium Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to chitin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cold Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to osmotic stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to oxidative stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to wounding Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform MAPK5a (identifier: Q10N20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform MAPK5b (identifier: Q10N20-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-173: Missing.

Note: Does not possess kinase activity.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Mitogen-activated protein kinase 5		PRO_0000239748
Regions
	Domain	36 – 322	287	Protein kinase
	Nucleotide binding	42 – 50	9	ATP By similarity
	Motif	194 – 196	3	TXY
Sites
	Active site	162	1	Proton acceptor By similarity
	Binding site	65	1	ATP By similarity
Amino acid modifications
	Modified residue	194	1	Phosphothreonine By similarity
	Modified residue	196	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	70 – 173	104	Missing in isoform MAPK5b.		VSP_019261
Experimental info
	Sequence conflict	46	1	A → G in CAC13967. Ref.3
	Sequence conflict	89	1	H → L in CAC13967. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform MAPK5a [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 417D81732635F2D3	FASTA	369	42,995
	10 20 30 40 50 60 MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV CSVMNFETRE 70 80 90 100 110 120 MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD VIPPPIPQAF NDVYIATELM 130 140 150 160 170 180 DTDLHHIIRS NQELSEEHCQ YFLYQILRGL KYIHSANVIH RDLKPSNLLL NANCDLKICD 190 200 210 220 230 240 FGLARPSSES DMMTEYVVTR WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR 250 260 270 280 290 300 DHMHQMRLIT EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE 310 320 330 340 350 360 RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED QMKQLIFNEA IEMNPNIRY
	Isoform MAPK5b [UniParc] [UniParc]. Checksum: 65AB271A79D98F8C	FASTA	265	30,817
	10 20 30 40 50 60 MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV CSVMNFETRE 70 80 90 100 110 120 MVAIKKIANC DLKICDFGLA RPSSESDMMT EYVVTRWYRA PELLLNSTDY SAAIDVWSVG 130 140 150 160 170 180 CIFMELINRQ PLFPGRDHMH QMRLITEVIG TPTDDELGFI RNEDARKYMR HLPQYPRRTF 190 200 210 220 230 240 ASMFPRVQPA ALDLIERMLT FNPLQRITVE EALDHPYLER LHDIADEPIC LEPFSFDFEQ 250 260 KALNEDQMKQ LIFNEAIEMN PNIRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues." Agrawal G.K., Rakwal R., Iwahashi H. Biochem. Biophys. Res. Commun. 294:1009-1016(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), INDUCTION. Strain: cv. Nipponbare. Tissue: Leaf.
[2]	"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice." Wen J.-Q., Oono K., Imai R. Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), ENZYME REGULATION, INTERACTION WITH MEK1, INDUCTION.
[3]	"Expression of Oryza sativa MAP kinase gene is developmentally regulated and stress-responsive." Huang H.-J., Fu S.-F., Tai Y.-H., Chou W.-C., Huang D.-D. Physiol. Plantarum 114:572-580(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), TISSUE SPECIFICITY, INDUCTION, PHOSPHORYLATION.
[4]	"Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase." Xiong L., Yang Y. Plant Cell 15:745-759(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MAPK5A AND MAPK5B), FUNCTION, ENZYME REGULATION, INDUCTION, ALTERNATIVE SPLICING.
[5]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[7]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[8]	"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection." Reyna N.S., Yang Y. Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ486975 mRNA. Translation: CAD31224.1. AF216315 mRNA. Translation: AAG40579.1. AJ250311 mRNA. Translation: CAC13967.1. AF479883 mRNA. Translation: AAL87689.1. AF479884 mRNA. Translation: AAL87690.1. AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation. DP000009 Genomic DNA. Translation: ABF95354.1. AP008209 Genomic DNA. Translation: BAF11684.1.
RefSeq	NP_001049770.1. NM_001056305.1.
UniGene	Os.406.
3D structure databases
ProteinModelPortal	Q10N20.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10N20. 1 interaction.
MINT	MINT-7234365.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g17700.1; LOC_Os03g17700.1; LOC_Os03g17700.
GeneID	4332475.
KEGG	dosa:Os03t0285800-01. osa:4332475.
Organism-specific databases
Gramene	Q10N20.
Phylogenomic databases
eggNOG	COG0515.
KO	K04371.
OMA	KYQPPIM.
Gene expression databases
ArrayExpress	Q10N20.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR008351. MAPK_JNK. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01772. JNKMAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPK5_ORYSJ

Accession

Primary (citable) accession number: Q10N20
Secondary accession number(s): Q7FNE2 , Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	August 22, 2006
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program