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Protein

Mitogen-activated protein kinase 5

Gene

MPK5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance (PubMed:12615946). Functions downstream of CPK18 in a signaling pathway that represses defense gene expression and negatively regulates resistance to rice blast fungus. Phosphorylated by CPK18 at Thr-14 and Thr-32 and activated independently of MAP kinase kinase (MKK) phosphorylation (PubMed:25035404).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated in response to low temperature (12 degrees Celsius) treatment (PubMed:12177502, PubMed:12615946). Activated by phosphorylation at Thr-14 and Thr-32 by CPK18 (PubMed:25035404).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATPPROSITE-ProRule annotation1
Active sitei162Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • camalexin biosynthetic process Source: EnsemblPlants
  • defense response to bacterium Source: UniProtKB
  • defense response to fungus Source: UniProtKB
  • inflorescence development Source: EnsemblPlants
  • plant ovule development Source: EnsemblPlants
  • pollen development Source: EnsemblPlants
  • pollen tube guidance Source: EnsemblPlants
  • positive regulation of response to salt stress Source: UniProtKB
  • priming of cellular response to stress Source: EnsemblPlants
  • regulation of gene expression Source: GO_Central
  • response to chitin Source: EnsemblPlants
  • response to cold Source: UniProtKB
  • response to freezing Source: EnsemblPlants
  • response to osmotic stress Source: EnsemblPlants
  • response to oxidative stress Source: EnsemblPlants
  • response to UV-B Source: EnsemblPlants
  • response to water deprivation Source: UniProtKB
  • response to wounding Source: EnsemblPlants

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPlant defense
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-198753 ERK/MAPK targets
R-OSA-198765 Signalling to ERK5
R-OSA-202670 ERKs are inactivated
R-OSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-OSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 5 (EC:2.7.11.24)
Short name:
MAP kinase 5
Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2
Gene namesi
Name:MPK5
Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
Ordered Locus Names:Os03g0285800, LOC_Os03g17700
ORF Names:OsJ_10412Imported, OSJNBa0013D02.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14T → A: Loss of phosphorylation. 1 Publication1
Mutagenesisi32T → A: Loss of phosphorylation. 1 Publication1
Mutagenesisi65K → R: Loss of autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397481 – 369Mitogen-activated protein kinase 5Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14Phosphothreonine1 Publication1
Modified residuei32Phosphothreonine1 Publication1
Modified residuei194PhosphothreonineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated at Thr-194 and Tyr-196, which activates the enzyme (By similarity). Autophosphorylated. Phosphorylated by CPK18 at Thr-14 and Thr-32, which activates the enzyme (PubMed:25035404).By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ10N20

PTM databases

iPTMnetiQ10N20

Expressioni

Tissue specificityi

Expressed in roots, stems and panicles, and at lower levels in leaves.1 Publication

Inductioni

By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors.5 Publications

Gene expression databases

ExpressionAtlasiQ10N20 differential
GenevisibleiQ10N20 OS

Interactioni

Subunit structurei

Interacts with MKK1 (PubMed:12177502). Interacts with CPK18 (PubMed:25035404).2 Publications

Protein-protein interaction databases

IntActiQ10N20, 2 interactors
MINTiQ10N20
STRINGi39947.LOC_Os03g17700.1

Structurei

3D structure databases

ProteinModelPortaliQ10N20
SMRiQ10N20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 196TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
InParanoidiQ10N20
KOiK20536
OMAiDVIDTIC
OrthoDBiEOG09360BWL

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008351 MAPK_JNK
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01772 JNKMAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform MAPK5a (identifier: Q10N20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV
60 70 80 90 100
CSVMNFETRE MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD
110 120 130 140 150
VIPPPIPQAF NDVYIATELM DTDLHHIIRS NQELSEEHCQ YFLYQILRGL
160 170 180 190 200
KYIHSANVIH RDLKPSNLLL NANCDLKICD FGLARPSSES DMMTEYVVTR
210 220 230 240 250
WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR DHMHQMRLIT
260 270 280 290 300
EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE
310 320 330 340 350
RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED
360
QMKQLIFNEA IEMNPNIRY
Length:369
Mass (Da):42,995
Last modified:August 22, 2006 - v1
Checksum:i417D81732635F2D3
GO
Isoform MAPK5b (identifier: Q10N20-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-173: Missing.

Note: Does not possess kinase activity.
Show »
Length:265
Mass (Da):30,817
Checksum:i65AB271A79D98F8C
GO

Sequence cautioni

The sequence AAO16999 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46A → G in CAC13967 (PubMed:11975731).Curated1
Sequence conflicti89H → L in CAC13967 (PubMed:11975731).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01926170 – 173Missing in isoform MAPK5b. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ486975 mRNA Translation: CAD31224.1
AF216315 mRNA Translation: AAG40579.1
AJ250311 mRNA Translation: CAC13967.1
AF479883 mRNA Translation: AAL87689.1
AF479884 mRNA Translation: AAL87690.1
AC134232 Genomic DNA Translation: AAO16999.1 Different initiation.
DP000009 Genomic DNA Translation: ABF95354.1
AP008209 Genomic DNA Translation: BAF11684.1
AP014959 Genomic DNA Translation: BAS83624.1
CM000140 Genomic DNA Translation: EEE58837.1
AK067339 mRNA Translation: BAG90374.1
AK104834 mRNA Translation: BAG96983.1
RefSeqiXP_015630496.1, XM_015775010.1 [Q10N20-1]
UniGeneiOs.406

Genome annotation databases

EnsemblPlantsiOs03t0285800-01; Os03t0285800-01; Os03g0285800 [Q10N20-1]
Os03t0285800-02; Os03t0285800-02; Os03g0285800 [Q10N20-1]
GeneIDi4332475
GrameneiOs03t0285800-01; Os03t0285800-01; Os03g0285800 [Q10N20-1]
Os03t0285800-02; Os03t0285800-02; Os03g0285800 [Q10N20-1]
KEGGiosa:4332475

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMPK5_ORYSJ
AccessioniPrimary (citable) accession number: Q10N20
Secondary accession number(s): B7EDC7
, Q7FNE2, Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 22, 2006
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health