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Q10N20

- MPK5_ORYSJ

UniProt

Q10N20 - MPK5_ORYSJ

Protein

Mitogen-activated protein kinase 5

Gene

MPK5

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to low temperature (12 degrees Celsius) treatment.By similarity2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei65 – 651ATPPROSITE-ProRule annotation
    Active sitei162 – 1621Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi42 – 509ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. activation of MAPK activity involved in osmosensory signaling pathway Source: EnsemblPlants/Gramene
    2. camalexin biosynthetic process Source: EnsemblPlants/Gramene
    3. defense response Source: UniProtKB-KW
    4. inflorescence development Source: EnsemblPlants/Gramene
    5. ovule development Source: EnsemblPlants/Gramene
    6. priming of cellular response to stress Source: EnsemblPlants/Gramene
    7. regulation of stomatal complex development Source: EnsemblPlants/Gramene
    8. regulation of stomatal complex patterning Source: EnsemblPlants/Gramene
    9. response to bacterium Source: EnsemblPlants/Gramene
    10. response to chitin Source: EnsemblPlants/Gramene
    11. response to osmotic stress Source: EnsemblPlants/Gramene
    12. response to oxidative stress Source: EnsemblPlants/Gramene
    13. response to UV-B Source: EnsemblPlants/Gramene
    14. response to wounding Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Plant defense

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205014. CREB phosphorylation through the activation of Ras.
    REACT_214549. Signalling to ERK5.
    REACT_215626. ERK2 activation.
    REACT_221522. ERKs are inactivated.
    REACT_221537. ERK1 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 5 (EC:2.7.11.24)
    Short name:
    MAP kinase 5
    Alternative name(s):
    Benzothiadiazole-induced MAP kinase 1
    MAP kinase 2
    Multiple stress-responsive MAP kinase 2
    OsBIMK1
    OsMAP1
    OsMAPK2
    OsMAPK5
    OsMPK3
    OsMSRMK2
    Gene namesi
    Name:MPK5
    Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
    Ordered Locus Names:Os03g0285800, LOC_Os03g17700
    ORF Names:OSJNBa0013D02.9
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ10N20.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Mitogen-activated protein kinase 5PRO_0000239748Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei194 – 1941PhosphothreonineBy similarity
    Modified residuei196 – 1961PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-194 and Tyr-196, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in roots, stems and panicles, and at lower levels in leaves.1 Publication

    Inductioni

    By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors.5 Publications

    Interactioni

    Subunit structurei

    Interacts with MKK1.1 Publication

    Protein-protein interaction databases

    IntActiQ10N20. 2 interactions.
    MINTiMINT-7234365.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10N20.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 322287Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi194 – 1963TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    KOiK04371.
    OMAiIADEPIC.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01772. JNKMAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform MAPK5a (identifier: Q10N20-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV    50
    CSVMNFETRE MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD 100
    VIPPPIPQAF NDVYIATELM DTDLHHIIRS NQELSEEHCQ YFLYQILRGL 150
    KYIHSANVIH RDLKPSNLLL NANCDLKICD FGLARPSSES DMMTEYVVTR 200
    WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR DHMHQMRLIT 250
    EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE 300
    RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED 350
    QMKQLIFNEA IEMNPNIRY 369
    Length:369
    Mass (Da):42,995
    Last modified:August 22, 2006 - v1
    Checksum:i417D81732635F2D3
    GO
    Isoform MAPK5b (identifier: Q10N20-2) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         70-173: Missing.

    Note: Does not possess kinase activity.

    Show »
    Length:265
    Mass (Da):30,817
    Checksum:i65AB271A79D98F8C
    GO

    Sequence cautioni

    The sequence AAO16999.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461A → G in CAC13967. (PubMed:11975731)Curated
    Sequence conflicti89 – 891H → L in CAC13967. (PubMed:11975731)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei70 – 173104Missing in isoform MAPK5b. 1 PublicationVSP_019261Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ486975 mRNA. Translation: CAD31224.1.
    AF216315 mRNA. Translation: AAG40579.1.
    AJ250311 mRNA. Translation: CAC13967.1.
    AF479883 mRNA. Translation: AAL87689.1.
    AF479884 mRNA. Translation: AAL87690.1.
    AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation.
    DP000009 Genomic DNA. Translation: ABF95354.1.
    AP008209 Genomic DNA. Translation: BAF11684.1.
    RefSeqiNP_001049770.1. NM_001056305.1. [Q10N20-1]
    UniGeneiOs.406.

    Genome annotation databases

    EnsemblPlantsiOS03T0285800-01; OS03T0285800-01; OS03G0285800. [Q10N20-1]
    OS03T0285800-02; OS03T0285800-02; OS03G0285800. [Q10N20-1]
    GeneIDi4332475.
    KEGGiosa:4332475.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ486975 mRNA. Translation: CAD31224.1 .
    AF216315 mRNA. Translation: AAG40579.1 .
    AJ250311 mRNA. Translation: CAC13967.1 .
    AF479883 mRNA. Translation: AAL87689.1 .
    AF479884 mRNA. Translation: AAL87690.1 .
    AC134232 Genomic DNA. Translation: AAO16999.1 . Different initiation.
    DP000009 Genomic DNA. Translation: ABF95354.1 .
    AP008209 Genomic DNA. Translation: BAF11684.1 .
    RefSeqi NP_001049770.1. NM_001056305.1. [Q10N20-1 ]
    UniGenei Os.406.

    3D structure databases

    ProteinModelPortali Q10N20.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q10N20. 2 interactions.
    MINTi MINT-7234365.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0285800-01 ; OS03T0285800-01 ; OS03G0285800 . [Q10N20-1 ]
    OS03T0285800-02 ; OS03T0285800-02 ; OS03G0285800 . [Q10N20-1 ]
    GeneIDi 4332475.
    KEGGi osa:4332475.

    Organism-specific databases

    Gramenei Q10N20.

    Phylogenomic databases

    eggNOGi COG0515.
    KOi K04371.
    OMAi IADEPIC.

    Enzyme and pathway databases

    Reactomei REACT_205014. CREB phosphorylation through the activation of Ras.
    REACT_214549. Signalling to ERK5.
    REACT_215626. ERK2 activation.
    REACT_221522. ERKs are inactivated.
    REACT_221537. ERK1 activation.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01772. JNKMAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues."
      Agrawal G.K., Rakwal R., Iwahashi H.
      Biochem. Biophys. Res. Commun. 294:1009-1016(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), INDUCTION.
      Strain: cv. Nipponbare.
      Tissue: Leaf.
    2. "Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
      Wen J.-Q., Oono K., Imai R.
      Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), ENZYME REGULATION, INTERACTION WITH MKK1, INDUCTION.
    3. "Expression of Oryza sativa MAP kinase gene is developmentally regulated and stress-responsive."
      Huang H.-J., Fu S.-F., Tai Y.-H., Chou W.-C., Huang D.-D.
      Physiol. Plantarum 114:572-580(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), TISSUE SPECIFICITY, INDUCTION, PHOSPHORYLATION.
    4. "Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase."
      Xiong L., Yang Y.
      Plant Cell 15:745-759(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MAPK5A AND MAPK5B), FUNCTION, ENZYME REGULATION, INDUCTION, ALTERNATIVE SPLICING.
    5. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    7. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    8. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
      Reyna N.S., Yang Y.
      Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, NOMENCLATURE.

    Entry informationi

    Entry nameiMPK5_ORYSJ
    AccessioniPrimary (citable) accession number: Q10N20
    Secondary accession number(s): Q7FNE2
    , Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3