Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q10N20 (MPK5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 5

Short name=MAP kinase 5
EC=2.7.11.24
Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2
Gene names
Name:MPK5
Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
Ordered Locus Names:Os03g0285800, LOC_Os03g17700
ORF Names:OSJNBa0013D02.9
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in disease resistance and abiotic stress tolerance signaling pathways. Acts as a positive regulator of drought, salt and cold tolerance. Negatively modulates pathogenesis-related (PR) gene expression and broad-spectrum disease resistance. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to low temperature (12 degrees Celsius) treatment. Ref.2 Ref.4

Subunit structure

Interacts with MKK1. Ref.2

Tissue specificity

Expressed in roots, stems and panicles, and at lower levels in leaves. Ref.3

Induction

By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea, by stresses, hormones, heavy metals, high/low temperature, UV-C and phosphatase inhibitors. Ref.1 Ref.2 Ref.3 Ref.4 Ref.8

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-194 and Tyr-196, which activates the enzyme By similarity. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAO16999.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPlant defense
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity involved in osmosensory signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

camalexin biosynthetic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

inflorescence development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

ovule development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

priming of cellular response to stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of stomatal complex development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of stomatal complex patterning

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to UV-B

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to bacterium

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to chitin

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to osmotic stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to oxidative stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to wounding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform MAPK5a (identifier: Q10N20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform MAPK5b (identifier: Q10N20-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-173: Missing.
Note: Does not possess kinase activity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Mitogen-activated protein kinase 5
PRO_0000239748

Regions

Domain36 – 322287Protein kinase
Nucleotide binding42 – 509ATP By similarity
Motif194 – 1963TXY

Sites

Active site1621Proton acceptor By similarity
Binding site651ATP By similarity

Amino acid modifications

Modified residue1941Phosphothreonine By similarity
Modified residue1961Phosphotyrosine By similarity

Natural variations

Alternative sequence70 – 173104Missing in isoform MAPK5b.
VSP_019261

Experimental info

Sequence conflict461A → G in CAC13967. Ref.3
Sequence conflict891H → L in CAC13967. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform MAPK5a [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 417D81732635F2D3

FASTA36942,995
        10         20         30         40         50         60 
MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV CSVMNFETRE 

        70         80         90        100        110        120 
MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD VIPPPIPQAF NDVYIATELM 

       130        140        150        160        170        180 
DTDLHHIIRS NQELSEEHCQ YFLYQILRGL KYIHSANVIH RDLKPSNLLL NANCDLKICD 

       190        200        210        220        230        240 
FGLARPSSES DMMTEYVVTR WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR 

       250        260        270        280        290        300 
DHMHQMRLIT EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE 

       310        320        330        340        350        360 
RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED QMKQLIFNEA 


IEMNPNIRY 

« Hide

Isoform MAPK5b [UniParc] [UniParc].

Checksum: 65AB271A79D98F8C
Show »

FASTA26530,817

References

« Hide 'large scale' references
[1]"Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues."
Agrawal G.K., Rakwal R., Iwahashi H.
Biochem. Biophys. Res. Commun. 294:1009-1016(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), INDUCTION.
Strain: cv. Nipponbare.
Tissue: Leaf.
[2]"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
Wen J.-Q., Oono K., Imai R.
Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), ENZYME REGULATION, INTERACTION WITH MKK1, INDUCTION.
[3]"Expression of Oryza sativa MAP kinase gene is developmentally regulated and stress-responsive."
Huang H.-J., Fu S.-F., Tai Y.-H., Chou W.-C., Huang D.-D.
Physiol. Plantarum 114:572-580(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAPK5A), TISSUE SPECIFICITY, INDUCTION, PHOSPHORYLATION.
[4]"Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase."
Xiong L., Yang Y.
Plant Cell 15:745-759(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MAPK5A AND MAPK5B), FUNCTION, ENZYME REGULATION, INDUCTION, ALTERNATIVE SPLICING.
[5]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[6]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[7]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[8]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ486975 mRNA. Translation: CAD31224.1.
AF216315 mRNA. Translation: AAG40579.1.
AJ250311 mRNA. Translation: CAC13967.1.
AF479883 mRNA. Translation: AAL87689.1.
AF479884 mRNA. Translation: AAL87690.1.
AC134232 Genomic DNA. Translation: AAO16999.1. Different initiation.
DP000009 Genomic DNA. Translation: ABF95354.1.
AP008209 Genomic DNA. Translation: BAF11684.1.
RefSeqNP_001049770.1. NM_001056305.1.
UniGeneOs.406.

3D structure databases

ProteinModelPortalQ10N20.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ10N20. 2 interactions.
MINTMINT-7234365.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0285800-01; OS03T0285800-01; OS03G0285800. [Q10N20-1]
OS03T0285800-02; OS03T0285800-02; OS03G0285800. [Q10N20-1]
GeneID4332475.
KEGGosa:4332475.

Organism-specific databases

GrameneQ10N20.

Phylogenomic databases

eggNOGCOG0515.
KOK04371.
OMADVIPPPL.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK5_ORYSJ
AccessionPrimary (citable) accession number: Q10N20
Secondary accession number(s): Q7FNE2 expand/collapse secondary AC list , Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 22, 2006
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations