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Q10MX3

- ASNS1_ORYSJ

UniProt

Q10MX3 - ASNS1_ORYSJ

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Protein
Asparagine synthetase [glutamine-hydrolyzing] 1
Gene
Os03g0291500, LOC_Os03g18130
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem By similarity.

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile By similarity
Binding sitei99 – 991Glutamine By similarity
Binding sitei232 – 2321ATP; via carbonyl oxygen By similarity
Binding sitei268 – 2681ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei344 – 3441Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi342 – 3432ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC

GO - Biological processi

  1. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  2. cellular response to sucrose starvation Source: EnsemblPlants/Gramene
  3. glutamine metabolic process Source: UniProtKB-KW
  4. response to fructose Source: EnsemblPlants/Gramene
  5. response to glucose Source: EnsemblPlants/Gramene
  6. response to sucrose Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Gene namesi
Ordered Locus Names:Os03g0291500, LOC_Os03g18130
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10MX3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 604603Asparagine synthetase [glutamine-hydrolyzing] 1
PRO_0000420842Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ10MX3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 186185Glutamine amidotransferase type-2
Add
BLAST
Domaini211 – 451241Asparagine synthetase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000027493.
OMAiKKVLWAI.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10MX3-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILAVLGA ADWSQAKRAH VLSCSRRLKH RGPDWSGLYQ CEGNFLAQQR    50
LAIVSPLSGD QPLYNADRTI VVVANGEIYN HKKIRKQFAS KHTFSTGSDC 100
EVIIPLYEEY GEDFVDMLDG VFAFVLYDTR TKTYMAARDA IGVNPLYIGR 150
GSDGAVWISS EMKALNEDCV EFEIFPPGHL YSSAAGGLRR WYKPQWFAEN 200
VPATPYQPLL LREAFEKAVI KRLMTDVPFG VLLSGGLDSS LVAAVTKRHL 250
IKTEAAEKFG AELHSFVVGL EGSPDLIAAR EVADHLGTIH HEFHFTVQDG 300
IDAIEEVIYH DETYDVTTIR ASTPMFLMAR KIKALGVKMV LSGEGSDELL 350
GGYLYFHFAP NKEEFHKETC RKVKALHQYD CLRANKATSA WGLEVRVPFL 400
DKEFINVAMS MDPEWKMYNA DLGRIEKWVM RKAFDDEEHP YLPKHILYRQ 450
KEQFSDGVGY NWIDGLKAFT EQQVSDEMMK NAAKVYPHNT PVNKEAYYYR 500
MIFERLFPQE SARETVPWGP SIACSTPAAI EWVEQWKASH DPSGRLIASH 550
NSASASANHT NHANANANGN SNGKANGNCA MAANGTNGVG LVVANGTANG 600
KMEA 604
Length:604
Mass (Da):67,322
Last modified:August 22, 2006 - v1
Checksum:i029B5770234C20FB
GO

Sequence cautioni

The sequence BAF11713.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC137634 Genomic DNA. No translation available.
DP000009 Genomic DNA. Translation: ABF95401.1.
AP008209 Genomic DNA. Translation: BAF11713.2. Sequence problems.
AK318591 mRNA. No translation available.
RefSeqiNP_001049799.2. NM_001056334.2.
UniGeneiOs.11327.

Genome annotation databases

EnsemblPlantsiOS03T0291500-01; OS03T0291500-01; OS03G0291500.
GeneIDi4332506.
KEGGiosa:4332506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC137634 Genomic DNA. No translation available.
DP000009 Genomic DNA. Translation: ABF95401.1 .
AP008209 Genomic DNA. Translation: BAF11713.2 . Sequence problems.
AK318591 mRNA. No translation available.
RefSeqi NP_001049799.2. NM_001056334.2.
UniGenei Os.11327.

3D structure databases

ProteinModelPortali Q10MX3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0291500-01 ; OS03T0291500-01 ; OS03G0291500 .
GeneIDi 4332506.
KEGGi osa:4332506.

Organism-specific databases

Gramenei Q10MX3.

Phylogenomic databases

HOGENOMi HOG000027493.
OMAi KKVLWAI.

Enzyme and pathway databases

UniPathwayi UPA00134 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Oryza sativa full length cDNA."
    The rice full-length cDNA consortium
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiASNS1_ORYSJ
AccessioniPrimary (citable) accession number: Q10MX3
Secondary accession number(s): Q0DSS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: August 22, 2006
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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