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Q10MX3

- ASNS1_ORYSJ

UniProt

Q10MX3 - ASNS1_ORYSJ

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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

Os03g0291500

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophilePROSITE-ProRule annotation
Binding sitei99 – 991GlutamineBy similarity
Binding sitei232 – 2321ATP; via carbonyl oxygenBy similarity
Binding sitei268 – 2681ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei344 – 3441Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi342 – 3432ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_250903. Amino acid synthesis and interconversion (transamination).
REACT_263402. ATF4 activates genes.
UniPathwayiUPA00134.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Gene namesi
Ordered Locus Names:Os03g0291500, LOC_Os03g18130
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10MX3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 604603Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000420842Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ10MX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 186185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini211 – 451241Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiQ10MX3.
OMAiKKVLWAI.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10MX3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILAVLGA ADWSQAKRAH VLSCSRRLKH RGPDWSGLYQ CEGNFLAQQR
60 70 80 90 100
LAIVSPLSGD QPLYNADRTI VVVANGEIYN HKKIRKQFAS KHTFSTGSDC
110 120 130 140 150
EVIIPLYEEY GEDFVDMLDG VFAFVLYDTR TKTYMAARDA IGVNPLYIGR
160 170 180 190 200
GSDGAVWISS EMKALNEDCV EFEIFPPGHL YSSAAGGLRR WYKPQWFAEN
210 220 230 240 250
VPATPYQPLL LREAFEKAVI KRLMTDVPFG VLLSGGLDSS LVAAVTKRHL
260 270 280 290 300
IKTEAAEKFG AELHSFVVGL EGSPDLIAAR EVADHLGTIH HEFHFTVQDG
310 320 330 340 350
IDAIEEVIYH DETYDVTTIR ASTPMFLMAR KIKALGVKMV LSGEGSDELL
360 370 380 390 400
GGYLYFHFAP NKEEFHKETC RKVKALHQYD CLRANKATSA WGLEVRVPFL
410 420 430 440 450
DKEFINVAMS MDPEWKMYNA DLGRIEKWVM RKAFDDEEHP YLPKHILYRQ
460 470 480 490 500
KEQFSDGVGY NWIDGLKAFT EQQVSDEMMK NAAKVYPHNT PVNKEAYYYR
510 520 530 540 550
MIFERLFPQE SARETVPWGP SIACSTPAAI EWVEQWKASH DPSGRLIASH
560 570 580 590 600
NSASASANHT NHANANANGN SNGKANGNCA MAANGTNGVG LVVANGTANG

KMEA
Length:604
Mass (Da):67,322
Last modified:August 22, 2006 - v1
Checksum:i029B5770234C20FB
GO

Sequence cautioni

The sequence BAF11713.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137634 Genomic DNA. No translation available.
DP000009 Genomic DNA. Translation: ABF95401.1.
AP008209 Genomic DNA. Translation: BAF11713.2. Sequence problems.
AK318591 mRNA. No translation available.
RefSeqiNP_001049799.2. NM_001056334.2.
UniGeneiOs.11327.

Genome annotation databases

EnsemblPlantsiOS03T0291500-01; OS03T0291500-01; OS03G0291500.
GeneIDi4332506.
KEGGiosa:4332506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137634 Genomic DNA. No translation available.
DP000009 Genomic DNA. Translation: ABF95401.1 .
AP008209 Genomic DNA. Translation: BAF11713.2 . Sequence problems.
AK318591 mRNA. No translation available.
RefSeqi NP_001049799.2. NM_001056334.2.
UniGenei Os.11327.

3D structure databases

ProteinModelPortali Q10MX3.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0291500-01 ; OS03T0291500-01 ; OS03G0291500 .
GeneIDi 4332506.
KEGGi osa:4332506.

Organism-specific databases

Gramenei Q10MX3.

Phylogenomic databases

HOGENOMi HOG000027493.
InParanoidi Q10MX3.
OMAi KKVLWAI.

Enzyme and pathway databases

UniPathwayi UPA00134 .
Reactomei REACT_250903. Amino acid synthesis and interconversion (transamination).
REACT_263402. ATF4 activates genes.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Oryza sativa full length cDNA."
    The rice full-length cDNA consortium
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiASNS1_ORYSJ
AccessioniPrimary (citable) accession number: Q10MX3
Secondary accession number(s): Q0DSS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: August 22, 2006
Last modified: November 26, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3