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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

Os03g0291500

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway: L-asparagine biosynthesis

This protein is involved in the pathway L-asparagine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophilePROSITE-ProRule annotation
Binding sitei99 – 991GlutamineBy similarity
Binding sitei232 – 2321ATP; via carbonyl oxygenBy similarity
Binding sitei268 – 2681ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei344 – 3441Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi342 – 3432ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_283821. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Gene namesi
Ordered Locus Names:Os03g0291500, LOC_Os03g18130
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 3

Organism-specific databases

GrameneiQ10MX3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 604603Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000420842Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g18130.1.

Structurei

3D structure databases

ProteinModelPortaliQ10MX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 186185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini211 – 451241Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiQ10MX3.
OMAiEKWVMRK.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10MX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAVLGA ADWSQAKRAH VLSCSRRLKH RGPDWSGLYQ CEGNFLAQQR
60 70 80 90 100
LAIVSPLSGD QPLYNADRTI VVVANGEIYN HKKIRKQFAS KHTFSTGSDC
110 120 130 140 150
EVIIPLYEEY GEDFVDMLDG VFAFVLYDTR TKTYMAARDA IGVNPLYIGR
160 170 180 190 200
GSDGAVWISS EMKALNEDCV EFEIFPPGHL YSSAAGGLRR WYKPQWFAEN
210 220 230 240 250
VPATPYQPLL LREAFEKAVI KRLMTDVPFG VLLSGGLDSS LVAAVTKRHL
260 270 280 290 300
IKTEAAEKFG AELHSFVVGL EGSPDLIAAR EVADHLGTIH HEFHFTVQDG
310 320 330 340 350
IDAIEEVIYH DETYDVTTIR ASTPMFLMAR KIKALGVKMV LSGEGSDELL
360 370 380 390 400
GGYLYFHFAP NKEEFHKETC RKVKALHQYD CLRANKATSA WGLEVRVPFL
410 420 430 440 450
DKEFINVAMS MDPEWKMYNA DLGRIEKWVM RKAFDDEEHP YLPKHILYRQ
460 470 480 490 500
KEQFSDGVGY NWIDGLKAFT EQQVSDEMMK NAAKVYPHNT PVNKEAYYYR
510 520 530 540 550
MIFERLFPQE SARETVPWGP SIACSTPAAI EWVEQWKASH DPSGRLIASH
560 570 580 590 600
NSASASANHT NHANANANGN SNGKANGNCA MAANGTNGVG LVVANGTANG

KMEA
Length:604
Mass (Da):67,322
Last modified:August 22, 2006 - v1
Checksum:i029B5770234C20FB
GO

Sequence cautioni

The sequence BAF11713.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137634 Genomic DNA. No translation available.
DP000009 Genomic DNA. Translation: ABF95401.1.
AP008209 Genomic DNA. Translation: BAF11713.2. Sequence problems.
AK318591 mRNA. No translation available.
RefSeqiNP_001049799.2. NM_001056334.2.
UniGeneiOs.11327.

Genome annotation databases

EnsemblPlantsiOS03T0291500-01; OS03T0291500-01; OS03G0291500.
GeneIDi4332506.
KEGGiosa:4332506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137634 Genomic DNA. No translation available.
DP000009 Genomic DNA. Translation: ABF95401.1.
AP008209 Genomic DNA. Translation: BAF11713.2. Sequence problems.
AK318591 mRNA. No translation available.
RefSeqiNP_001049799.2. NM_001056334.2.
UniGeneiOs.11327.

3D structure databases

ProteinModelPortaliQ10MX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g18130.1.

Protein family/group databases

MEROPSiC44.976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0291500-01; OS03T0291500-01; OS03G0291500.
GeneIDi4332506.
KEGGiosa:4332506.

Organism-specific databases

GrameneiQ10MX3.

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiQ10MX3.
OMAiEKWVMRK.

Enzyme and pathway databases

UniPathwayiUPA00134.
ReactomeiREACT_283821. Amino acid synthesis and interconversion (transamination).

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Oryza sativa full length cDNA."
    The rice full-length cDNA consortium
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiASNS1_ORYSJ
AccessioniPrimary (citable) accession number: Q10MX3
Secondary accession number(s): Q0DSS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: August 22, 2006
Last modified: June 24, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.