Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q10MX3

- ASNS1_ORYSJ

UniProt

Q10MX3 - ASNS1_ORYSJ

Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

Os03g0291500

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21NucleophilePROSITE-ProRule annotation
    Binding sitei99 – 991GlutamineBy similarity
    Binding sitei232 – 2321ATP; via carbonyl oxygenBy similarity
    Binding sitei268 – 2681ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei344 – 3441Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi342 – 3432ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to sucrose starvation Source: EnsemblPlants/Gramene
    2. glutamine metabolic process Source: UniProtKB-KW
    3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
    4. response to fructose Source: EnsemblPlants/Gramene
    5. response to glucose Source: EnsemblPlants/Gramene
    6. response to sucrose Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase 1
    Gene namesi
    Ordered Locus Names:Os03g0291500, LOC_Os03g18130
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ10MX3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 604603Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000420842Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ10MX3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 186185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini211 – 451241Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    HOGENOMiHOG000027493.
    OMAiKKVLWAI.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q10MX3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGILAVLGA ADWSQAKRAH VLSCSRRLKH RGPDWSGLYQ CEGNFLAQQR    50
    LAIVSPLSGD QPLYNADRTI VVVANGEIYN HKKIRKQFAS KHTFSTGSDC 100
    EVIIPLYEEY GEDFVDMLDG VFAFVLYDTR TKTYMAARDA IGVNPLYIGR 150
    GSDGAVWISS EMKALNEDCV EFEIFPPGHL YSSAAGGLRR WYKPQWFAEN 200
    VPATPYQPLL LREAFEKAVI KRLMTDVPFG VLLSGGLDSS LVAAVTKRHL 250
    IKTEAAEKFG AELHSFVVGL EGSPDLIAAR EVADHLGTIH HEFHFTVQDG 300
    IDAIEEVIYH DETYDVTTIR ASTPMFLMAR KIKALGVKMV LSGEGSDELL 350
    GGYLYFHFAP NKEEFHKETC RKVKALHQYD CLRANKATSA WGLEVRVPFL 400
    DKEFINVAMS MDPEWKMYNA DLGRIEKWVM RKAFDDEEHP YLPKHILYRQ 450
    KEQFSDGVGY NWIDGLKAFT EQQVSDEMMK NAAKVYPHNT PVNKEAYYYR 500
    MIFERLFPQE SARETVPWGP SIACSTPAAI EWVEQWKASH DPSGRLIASH 550
    NSASASANHT NHANANANGN SNGKANGNCA MAANGTNGVG LVVANGTANG 600
    KMEA 604
    Length:604
    Mass (Da):67,322
    Last modified:August 22, 2006 - v1
    Checksum:i029B5770234C20FB
    GO

    Sequence cautioni

    The sequence BAF11713.2 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137634 Genomic DNA. No translation available.
    DP000009 Genomic DNA. Translation: ABF95401.1.
    AP008209 Genomic DNA. Translation: BAF11713.2. Sequence problems.
    AK318591 mRNA. No translation available.
    RefSeqiNP_001049799.2. NM_001056334.2.
    UniGeneiOs.11327.

    Genome annotation databases

    EnsemblPlantsiOS03T0291500-01; OS03T0291500-01; OS03G0291500.
    GeneIDi4332506.
    KEGGiosa:4332506.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137634 Genomic DNA. No translation available.
    DP000009 Genomic DNA. Translation: ABF95401.1 .
    AP008209 Genomic DNA. Translation: BAF11713.2 . Sequence problems.
    AK318591 mRNA. No translation available.
    RefSeqi NP_001049799.2. NM_001056334.2.
    UniGenei Os.11327.

    3D structure databases

    ProteinModelPortali Q10MX3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0291500-01 ; OS03T0291500-01 ; OS03G0291500 .
    GeneIDi 4332506.
    KEGGi osa:4332506.

    Organism-specific databases

    Gramenei Q10MX3.

    Phylogenomic databases

    HOGENOMi HOG000027493.
    OMAi KKVLWAI.

    Enzyme and pathway databases

    UniPathwayi UPA00134 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "Oryza sativa full length cDNA."
      The rice full-length cDNA consortium
      Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiASNS1_ORYSJ
    AccessioniPrimary (citable) accession number: Q10MX3
    Secondary accession number(s): Q0DSS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3