Reviewed, UniProtKB/Swiss-Prot Q10KF0 (PSA2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-2
    EC=3.4.25.1
Alternative name(s):
    20S proteasome alpha subunit B
    20S proteasome subunit alpha-2

Gene names

Name:	PAB1
Ordered Locus Names:	Os03g0387100, LOC_Os03g26970
ORF Names:	OSJNBb0058G04.11

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Proteasome subunit alpha type-2		PRO_0000124087

Sequences

	Sequence		Length	Mass (Da)
	Q10KF0-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: D98998C0BC6CD676	FASTA	235	25,844
	10 20 30 40 50 60 MGDSQYSFSL TTFSPSGKLV QIEHALTAVG SGQTSLGIKA ANGVVIATEK KLPSILVDET 70 80 90 100 110 120 SVQKIQSLTP NIGVVYSGMG PDFRVLVRKS RKQAQQYYRL YKETIPVTQL VRETAAVMQE 130 140 150 160 170 180 FTQSGGVRPF GVSLLIAGYD DNGPQLYQVD PSGSYFSWKA SAMGKNVSNA KTFLEKRYTE 190 200 210 220 230 DMELDDAIHT AILTLKEGYE GQISANNIEI GIIRSDREFK VLSPAEIKDF LEEVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structural features of the 20S proteasome subunits of rice (Oryza sativa)." Sassa H., Oguchi S., Inoue T., Hirano H. Gene 250:61-66(2000) [PubMed: 10854779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026559 mRNA. Translation: BAA96830.1. AC103551 Genomic DNA. Translation: AAT78811.1. DP000009 Genomic DNA. Translation: ABF96319.1. AP008209 Genomic DNA. Translation: BAF12173.1.
RefSeq	NP_001050259.1.
UniGene	Os.7900
3D structure databases
SMR	Q10KF0. Positions 6-234.
ModBase	Search...
Protein family/group databases
MEROPS	T01.972.
Genome annotation databases
GeneID	4333000.
GenomeReviews	Gene locus PAB1 in contig AP008209_GR.
KEGG	osa:4333000.
Organism-specific databases
Gramene	Q9LSU2.
Phylogenomic databases
HOGENOM	HBG499923.
PhylomeDB	Q10KF0.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA2_ORYSJ

Accession

Primary (citable) accession number: Q10KF0
Secondary accession number(s): O22539, Q6AVF6, Q9LSU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	August 22, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)