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Protein

Pyruvate dehydrogenase E1 component subunit beta-4, chloroplastic

Gene

Os03g0645100

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136Thiamine pyrophosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandPyruvate, Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta-4, chloroplastic (EC:1.2.4.1)
Gene namesi
Ordered Locus Names:Os03g0645100, LOC_Os03g44300
ORF Names:OsJ_11888
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 57ChloroplastSequence analysisAdd BLAST57
ChainiPRO_000042137658 – 400Pyruvate dehydrogenase E1 component subunit beta-4, chloroplasticAdd BLAST343

Proteomic databases

PaxDbiQ10G39.

Expressioni

Gene expression databases

ExpressionAtlasiQ10G39. baseline and differential.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os03g44300.1.

Structurei

3D structure databases

SMRiQ10G39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0524. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281450.
InParanoidiQ10G39.
KOiK00162.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
InterProiView protein in InterPro
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
PfamiView protein in Pfam
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
SMARTiView protein in SMART
SM00861. Transket_pyr. 1 hit.
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q10G39-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAASSLHAA PRVGSSSSFS SSSSAGRRSA SAARSVRVAA AAGSCAARRA
60 70 80 90 100
GGRMVARAAV ASKAESPASA ASSKSDGHEV LLFEALREAL IEEMKEDPTV
110 120 130 140 150
CVFGEDVGHY GGSYKVTKGL AEMFGDLRVL DTPIAENSFT GMGVGAAMKG
160 170 180 190 200
LRPVVEGMNM GFLLLAYNQI SNNCGMLHYT SGGQFKIPIV IRGPGGVGRQ
210 220 230 240 250
LGAEHSQRLE SYFQSIPGLQ MVACSTPYNA KGLMKAAIRS ENPVVLFEHV
260 270 280 290 300
LLYNLKEKIP DEEYVLCLEE AEMVRPGEHV TILTYSRMRY HVMQAAKTLV
310 320 330 340 350
NKGYDPEVID IRSLKPFDLH TIGNSIKKTH RVLIVEECMR TGGIGASLRS
360 370 380 390 400
AIIDNFWDYL DAPIMCLSSQ DVPTPYAAPL EDATVVQPAQ IVAAVEQICQ
Length:400
Mass (Da):42,876
Last modified:August 22, 2006 - v1
Checksum:i570C50A0E1AF1C84
GO
Isoform 2 (identifier: Q10G39-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Note: May be due to an intron retention.
Show »
Length:307
Mass (Da):33,747
Checksum:i969A93FC2BBA1427
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0456391 – 93Missing in isoform 2. 1 PublicationAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000009 Genomic DNA. Translation: ABF97865.1.
DP000009 Genomic DNA. Translation: ABF97866.1.
AP008209 Genomic DNA. Translation: BAF12677.1.
AP014959 Genomic DNA. No translation available.
CM000140 Genomic DNA. Translation: EEE59584.1.
AK069197 mRNA. Translation: BAG91310.1.
AK103256 mRNA. Translation: BAG95979.1.
RefSeqiXP_015631374.1. XM_015775888.1. [Q10G39-1]
UniGeneiOs.87910.

Genome annotation databases

EnsemblPlantsiOS03T0645100-01; OS03T0645100-01; OS03G0645100.
GeneIDi4333564.
GrameneiOS03T0645100-01; OS03T0645100-01; OS03G0645100.
KEGGiosa:4333564.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiODPB4_ORYSJ
AccessioniPrimary (citable) accession number: Q10G39
Secondary accession number(s): Q10G38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: August 22, 2006
Last modified: December 20, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations