Q10CQ1 (MAD14_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MADS-box transcription factor 14

Alternative name(s):
FDRMADS6
OsMADS14
Protein AGAMOUS-like 10
Protein APETALA1-like B
RMADS211

Gene names

Name:	MADS14
Synonyms:	AGL10, RAP1B
Ordered Locus Names:	Os03g0752800, LOC_Os03g54160
ORF Names:	OJ1112_G08.13, OSJNBa0032E21.04, OSJNBa0047E24.2

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor. May be involved in the control of flowering time. Ref.7
Subunit structure	May interact with the K-box of MADS1 and MADS6.
Subcellular location	Nucleus Probable.
Tissue specificity	Highly expressed in sterile lemmas, at intermediate levels in stamens, and weakly in lemmas, paleas and carpels. Ref.1
Developmental stage	Expressed at early stage of flower development in the spikelet (rice flower) apical meristem and later in developing stamens, pistil primordia and differentiated anthers.
Sequence similarities	Contains 1 K-box domain. Contains 1 MADS-box domain.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MADS1	Q10PZ9	4	EBI-627890,EBI-627957
MADS6	Q6EU39	5	EBI-627890,EBI-627980
MADS7	Q0J466	4	EBI-627890,EBI-627872

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q10CQ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q10CQ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     171-171: E → ENPCSFLQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	MADS-box transcription factor 14		PRO_0000229899
Regions
	Domain	1 – 61	61	MADS-box
	Domain	88 – 178	91	K-box
Natural variations
	Alternative sequence	171	1	E → ENPCSFLQ in isoform 2.		VSP_017783
Experimental info
	Sequence conflict	27	1	G → K in AAF19047. Ref.1
	Sequence conflict	183	1	V → L in BAA94342. Ref.2
	Sequence conflict	193	1	S → I in BAA94342. Ref.2
	Sequence conflict	225	1	A → P in BAA94342. Ref.2
	Sequence conflict	232	1	V → E in AAF19047. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 80CF6A3CAFB4084C	FASTA	246	28,423
	10 20 30 40 50 60 MGRGKVQLKR IENKINRQVT FSKRRSGLLK KANEISVLCD AEVALIIFST KGKLYEYATD 70 80 90 100 110 120 SCMDKILERY ERYSYAEKVL ISAESDTQGN WCHEYRKLKA KVETIQKCQK HLMGEDLESL 130 140 150 160 170 180 NLKELQQLEQ QLENSLKHIR SRKSQLMLES INELQRKEKS LQEENKVLQK ELVEKQKVQK 190 200 210 220 230 240 QQVQWDQTQP QTSSSSSSFM MREALPTTNI SNYPAAAGER IEDVAAGQPQ HVRIGLPPWM LSHING
	Isoform 2 [UniParc] [UniParc]. Checksum: E1A2118F30DEC03D	FASTA	253	29,212
	10 20 30 40 50 60 MGRGKVQLKR IENKINRQVT FSKRRSGLLK KANEISVLCD AEVALIIFST KGKLYEYATD 70 80 90 100 110 120 SCMDKILERY ERYSYAEKVL ISAESDTQGN WCHEYRKLKA KVETIQKCQK HLMGEDLESL 130 140 150 160 170 180 NLKELQQLEQ QLENSLKHIR SRKSQLMLES INELQRKEKS LQEENKVLQK ENPCSFLQLV 190 200 210 220 230 240 EKQKVQKQQV QWDQTQPQTS SSSSSFMMRE ALPTTNISNY PAAAGERIED VAAGQPQHVR 250 IGLPPWMLSH ING

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system." Moon Y.-H., Kang H.-G., Jung J.-Y., Jeon J.-S., Sung S.-K., An G. Plant Physiol. 120:1193-1204(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH MADS6. Tissue: Flower.
[2]	"Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes." Kyozuka J., Kobayashi T., Morita M., Shimamoto K. Plant Cell Physiol. 41:710-718(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Panicle.
[3]	"Isolation and characterization of rice MADS box gene homologs." Yao Q., Peng R., Xiong A. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[4]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[7]	"Production of transgenic rice plants showing reduced heading date and plant height by ectopic expression of rice MADS-box genes." Jeon J.-S., Lee S., Nam J., Jung K.-H., Yang W.-S., Yi G.-H., Oh B.-G., An G. Mol. Breed. 6:581-592(2000) [AGRICOLA] [Europe PMC] Cited for: FUNCTION.
[8]	"Two rice MADS domain proteins interact with OsMADS1." Lim J., Moon Y.-H., An G., Jang S.K. Plant Mol. Biol. 44:513-527(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MADS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF058697 mRNA. Translation: AAF19047.1. AB041020 mRNA. Translation: BAA94342.1. AY332478 mRNA. Translation: AAQ01164.1. AY551916 mRNA. Translation: AAS59822.1. AC092556 Genomic DNA. Translation: AAR87240.1. AC135225 Genomic DNA. Translation: AAP68361.1. AF377947 Genomic DNA. Translation: AAM34398.1. DP000009 Genomic DNA. Translation: ABF98924.1. DP000009 Genomic DNA. Translation: ABF98925.1. AP008209 Genomic DNA. Translation: BAF13214.1.
RefSeq	NP_001051300.1. NM_001057835.1.
UniGene	Os.2348.
3D structure databases
ProteinModelPortal	Q10CQ1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10CQ1. 12 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g54160.2; LOC_Os03g54160.2; LOC_Os03g54160.
GeneID	4334140.
KEGG	osa:4334140.
Organism-specific databases
Gramene	Q10CQ1.
Phylogenomic databases
eggNOG	COG5068.
KO	K09264.
ProtClustDB	CLSN2694303.
Gene expression databases
ArrayExpress	Q10CQ1.
Family and domain databases
InterPro	IPR002487. TF_Kbox. IPR002100. TF_MADSbox. [Graphical view]
Pfam	PF01486. K-box. 1 hit. PF00319. SRF-TF. 1 hit. [Graphical view]
PRINTS	PR00404. MADSDOMAIN.
SMART	SM00432. MADS. 1 hit. [Graphical view]
SUPFAM	SSF55455. TF_MADSbox. 1 hit.
PROSITE	PS51297. K_BOX. 1 hit. PS00350. MADS_BOX_1. 1 hit. PS50066. MADS_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAD14_ORYSJ

Accession

Primary (citable) accession number: Q10CQ1
Secondary accession number(s): Q10CQ2 , Q7Y023, Q8LLN3, Q9M7C6, Q9MAY7, Q9SEX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program