ID   GSTU1_ORYSJ             Reviewed;         231 AA.
AC   Q10CE7; B7EUJ2; O65032;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   27-MAR-2024, entry version 111.
DE   RecName: Full=Probable glutathione S-transferase GSTU1;
DE            EC=2.5.1.18;
GN   Name=GSTU1; Synonyms=GST1;
GN   OrderedLocusNames=Os03g0785900, LOC_Os03g57200; ORFNames=OsJ_012308;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15069639; DOI=10.1007/s00438-004-1006-8;
RA   Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C.;
RT   "Organisation and structural evolution of the rice glutathione S-
RT   transferase gene family.";
RL   Mol. Genet. Genomics 271:511-521(2004).
RN   [8]
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE.
RX   PubMed=12692133; DOI=10.1074/jbc.m303620200;
RA   Dixon D.P., McEwen A.G., Lapthorn A.J., Edwards R.;
RT   "Forced evolution of a herbicide detoxifying glutathione transferase.";
RL   J. Biol. Chem. 278:23930-23935(2003).
CC   -!- FUNCTION: Conjugation of reduced glutathione to a wide number of
CC       exogenous and endogenous hydrophobic electrophiles.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- SIMILARITY: Belongs to the GST superfamily. Tau family. {ECO:0000305}.
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DR   EMBL; DP000009; ABF99228.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF13399.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS86728.1; -; Genomic_DNA.
DR   EMBL; CM000140; EAZ28825.1; -; Genomic_DNA.
DR   EMBL; AK103358; BAG96039.1; -; mRNA.
DR   RefSeq; XP_015630984.1; XM_015775498.1.
DR   PDB; 1OYJ; X-ray; 1.95 A; A/B/C/D=1-231.
DR   PDBsum; 1OYJ; -.
DR   AlphaFoldDB; Q10CE7; -.
DR   SMR; Q10CE7; -.
DR   STRING; 39947.Q10CE7; -.
DR   PaxDb; 39947-Q10CE7; -.
DR   EnsemblPlants; Os03t0785900-01; Os03t0785900-01; Os03g0785900.
DR   GeneID; 4334347; -.
DR   Gramene; Os03t0785900-01; Os03t0785900-01; Os03g0785900.
DR   KEGG; osa:4334347; -.
DR   eggNOG; KOG0406; Eukaryota.
DR   HOGENOM; CLU_011226_18_2_1; -.
DR   InParanoid; Q10CE7; -.
DR   OMA; YYSWFEA; -.
DR   OrthoDB; 767442at2759; -.
DR   EvolutionaryTrace; Q10CE7; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   CDD; cd03185; GST_C_Tau; 1.
DR   CDD; cd03058; GST_N_Tau; 1.
DR   Gene3D; 1.20.1050.10; -; 1.
DR   Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR045074; GST_C_Tau.
DR   InterPro; IPR045073; Omega/Tau-like.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11260:SF732; GLUTATHIONE S-TRANSFERASE GSTU1-RELATED; 1.
DR   PANTHER; PTHR11260; GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING; 1.
DR   Pfam; PF13410; GST_C_2; 1.
DR   Pfam; PF13417; GST_N_3; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR   SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR   SUPFAM; SSF52833; Thioredoxin-like; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
DR   Genevisible; Q10CE7; OS.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Transferase.
FT   CHAIN           1..231
FT                   /note="Probable glutathione S-transferase GSTU1"
FT                   /id="PRO_0000185869"
FT   DOMAIN          5..84
FT                   /note="GST N-terminal"
FT   DOMAIN          97..220
FT                   /note="GST C-terminal"
FT   BINDING         15
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000269|PubMed:12692133"
FT   BINDING         42
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000269|PubMed:12692133"
FT   BINDING         56
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000269|PubMed:12692133"
FT   BINDING         68..69
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT   STRAND          7..11
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           16..28
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   STRAND          33..36
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           44..49
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   TURN            51..53
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   STRAND          58..61
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   STRAND          64..68
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           69..79
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           97..124
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           127..148
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   STRAND          152..158
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           164..169
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           170..175
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           176..183
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           187..190
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           192..201
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           205..210
FT                   /evidence="ECO:0007829|PDB:1OYJ"
FT   HELIX           214..229
FT                   /evidence="ECO:0007829|PDB:1OYJ"
SQ   SEQUENCE   231 AA;  25871 MW;  5E0F3E78F542B739 CRC64;
     MAEEKELVLL DFWVSPFGQR CRIAMAEKGL EFEYREEDLG NKSDLLLRSN PVHRKIPVLL
     HAGRPVSESL VILQYLDDAF PGTPHLLPPA NSGDADAAYA RATARFWADY VDRKLYDCGS
     RLWRLKGEPQ AAAGREMAEI LRTLEAELGD REFFGGGGGG RLGFVDVALV PFTAWFYSYE
     RCGGFSVEEV APRLAAWARR CGRIDSVVKH LPSPEKVYDF VGVLKKKYGV E
//