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Q10CE4

- GLO1_ORYSJ

UniProt

Q10CE4 - GLO1_ORYSJ

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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO1

Gene

GLO1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation.2 Publications

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.1 Publication

Cofactori

FMN.PROSITE-ProRule annotation

Kineticsi

  1. KM=0.4 mM for glycolate (at pH 8.0 and 30 degrees Celsius)1 Publication
  2. KM=4 mM for glyoxylate (at pH 8.0 and 30 degrees Celsius)1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251SubstratePROSITE-ProRule annotation
Binding sitei107 – 1071FMNPROSITE-ProRule annotation
Binding sitei128 – 1281FMNPROSITE-ProRule annotation
Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
Binding sitei156 – 1561FMNPROSITE-ProRule annotation
Binding sitei165 – 1651SubstratePROSITE-ProRule annotation
Binding sitei231 – 2311FMNPROSITE-ProRule annotation
Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
Binding sitei258 – 2581SubstratePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 31025FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. glycolate oxidase activity Source: UniProtKB
  3. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
  4. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
  5. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. modulation by virus of host morphology or physiology Source: UniProtKB
  2. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
  3. photorespiration Source: UniProtKB
  4. regulation of photosynthesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Host-virus interaction, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 1
Short name:
GOX 1
Short name:
OsGLO1
Short chain alpha-hydroxy acid oxidase GLO1
Gene namesi
Name:GLO1
Synonyms:GOX
Ordered Locus Names:Os03g0786100, LOC_Os03g57220
ORF Names:OsJ_12861
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10CE4.

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Peroxisomal (S)-2-hydroxy-acid oxidase GLO1PRO_0000403409Add
BLAST

Proteomic databases

PaxDbiQ10CE4.

Expressioni

Tissue specificityi

Expressed constitutively in leaves (at protein level).1 Publication

Interactioni

Subunit structurei

Homotetramer or homooctamer (By similarity). Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ10CE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 360360FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi367 – 3693Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1304.
InParanoidiQ10CE4.
KOiK11517.
OMAiHALWAPR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10CE4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGEITNVMEY QAIAKQKLPK MIYDYYASGA EDEWTLKENR EAFSRILFRP
60 70 80 90 100
RILIDVSKID MSATVLGFKI SMPIMIAPSA MQKMAHPDGE YATARAASAA
110 120 130 140 150
GTIMTLSSWA TSSVEEVAST GPGIRFFQLY VYKDRNVVEQ LVRRAERAGF
160 170 180 190 200
KAIALTVDTP RLGRREADIK NRFVLPPYLT LKNFEGLDLA EMDKSNDSGL
210 220 230 240 250
ASYVAGQIDR TLSWKDVKWL QSITSLPILV KGVITAEDAR LAVHSGAAGI
260 270 280 290 300
IVSNHGARQL DYVPATISAL EEVVTAAAGR IPVYLDGGVR RGTDVFKALA
310 320 330 340 350
LGAAGVFIGR PVVFALAAEG EAGVRNVLRM MREEFELTMA LSGCTSLADI
360
TRAHIYTDAD RLARPFPRL
Length:369
Mass (Da):40,384
Last modified:August 22, 2006 - v1
Checksum:iB14AF6A0E95883FF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000009 Genomic DNA. Translation: ABF99231.1.
AP008209 Genomic DNA. Translation: BAF13401.1.
CM000140 Genomic DNA. Translation: EEE60058.1.
AK098878 mRNA. Translation: BAG93788.1.
AK120304 mRNA. Translation: BAG99960.1.
RefSeqiNP_001051487.1. NM_001058022.1.
UniGeneiOs.74656.

Genome annotation databases

EnsemblPlantsiOS03T0786100-01; OS03T0786100-01; OS03G0786100.
OS03T0786100-02; OS03T0786100-02; OS03G0786100.
GeneIDi4334349.
KEGGiosa:4334349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000009 Genomic DNA. Translation: ABF99231.1 .
AP008209 Genomic DNA. Translation: BAF13401.1 .
CM000140 Genomic DNA. Translation: EEE60058.1 .
AK098878 mRNA. Translation: BAG93788.1 .
AK120304 mRNA. Translation: BAG99960.1 .
RefSeqi NP_001051487.1. NM_001058022.1.
UniGenei Os.74656.

3D structure databases

ProteinModelPortali Q10CE4.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q10CE4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0786100-01 ; OS03T0786100-01 ; OS03G0786100 .
OS03T0786100-02 ; OS03T0786100-02 ; OS03G0786100 .
GeneIDi 4334349.
KEGGi osa:4334349.

Organism-specific databases

Gramenei Q10CE4.

Phylogenomic databases

eggNOGi COG1304.
InParanoidi Q10CE4.
KOi K11517.
OMAi HALWAPR.

Enzyme and pathway databases

UniPathwayi UPA00951 ; UER00912 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view ]
Pfami PF01070. FMN_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Oxalate accumulation and regulation is independent of glycolate oxidase in rice leaves."
    Xu H.-W., Ji X.-M., He Z.-H., Shi W.-P., Zhu G.-H., Niu J.-K., Li B.-S., Peng X.-X.
    J. Exp. Bot. 57:1899-1908(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, FUNCTION.
    Strain: cv. Shishoubaimao and cv. Xiangzhongxian 2.
  7. "Interaction of rice dwarf virus outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8."
    Zhou F., Wu G., Deng W., Pu Y., Wei C., Li Y.
    FEBS Lett. 581:34-40(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RICE DWARF VIRUS P8, SUBCELLULAR LOCATION.
    Strain: cv. Xiushui 11.
  8. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
    Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
    J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Shishoubaimao.

Entry informationi

Entry nameiGLO1_ORYSJ
AccessioniPrimary (citable) accession number: Q10CE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: August 22, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3