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Q10B67 (MAN4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase 4

EC=3.2.1.78
Alternative name(s):
Beta-mannanase 4
Endo-beta-1,4-mannanase 4
OsMAN4
Gene names
Name:MAN4
Ordered Locus Names:Os03g0828500, LOC_Os03g61280
ORF Names:OSJNBa0010E04.2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Tissue specificity

Ubiquitous. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q10B67-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q10B67-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 461461Mannan endo-1,4-beta-mannosidase 4
PRO_0000277485

Sites

Active site1961Proton donor By similarity
Active site3141Nucleophile By similarity

Natural variations

Alternative sequence1 – 2222Missing in isoform 2.
VSP_023021

Experimental info

Sequence conflict4211G → S in AK106915. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: 872068A0D8DDD08F

FASTA46150,739
        10         20         30         40         50         60 
MSSCALVQRP PPWRAAVAPG DGMVAVDGTQ FVVDCGRTIF FSGFNAYWLM MMAADPALRG 

        70         80         90        100        110        120 
AVATAFQQAS AHGLNLARTW AFSDGGDQPL QSSPGVYNET MFQGLDFVIA EARRHGIYLL 

       130        140        150        160        170        180 
LCLTNNFDNF GGKRQYVRWA GDAGHNLTSD DDFFTSTIVK SYFKNHVKTV LTRVNTLTGV 

       190        200        210        220        230        240 
AYKDDPTIFA WELMNEPRCY ADPTGAMVQA WVEEMAPYVK SVDGRHLVTP GLEGFYGAGE 

       250        260        270        280        290        300 
HESKELNPWG IYYGTNYVAT HRTAAVDFAT IHLYPDVWLW GSSADEQATF FRNWTRSHID 

       310        320        330        340        350        360 
ATAAYLGMPL LVTEYGKFLW KEVGANKAQR NYFLDLVLDA IYASASRGGP LVGGAFWQLL 

       370        380        390        400        410        420 
LDGDIVAGMD SLRDGYEIIL AEDSRAASII GEHSEQLAAL NGQDADVLCR RASSHRRTRL 

       430        440        450        460 
GNSLSCGGGD TLELLLRMVL ACFVSLSRSI SSFIVQNFIL L 

« Hide

Isoform 2 [UniParc].

Checksum: 881013E73BC3D91D
Show »

FASTA43948,491

References

« Hide 'large scale' references
[1]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Nipponbare.
[5]"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC096687 Genomic DNA. Translation: AAL79758.1.
DP000009 Genomic DNA. Translation: ABF99676.1.
DP000009 Genomic DNA. Translation: ABF99677.1.
AP008209 Genomic DNA. Translation: BAF13691.1.
AK106915 mRNA. No translation available.
RefSeqNP_001051777.1. NM_001058312.1.
UniGeneOs.7260.

3D structure databases

ProteinModelPortalQ10B67.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0828500-01; OS03T0828500-01; OS03G0828500. [Q10B67-1]
GeneID4334653.
KEGGosa:4334653.

Organism-specific databases

GrameneQ10B67.

Phylogenomic databases

eggNOGCOG3934.
HOGENOMHOG000169952.
OMALNLARTW.
ProtClustDBCLSN2694492.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMAN4_ORYSJ
AccessionPrimary (citable) accession number: Q10B67
Secondary accession number(s): Q10B68, Q8SAY2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries