Reviewed, UniProtKB/Swiss-Prot Q10B67 (MAN4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Mannan endo-1,4-beta-mannosidase 4
    EC=3.2.1.78
Alternative name(s):
    Beta-mannanase 4
    Endo-beta-1,4-mannanase 4
    OsMAN4

Gene names

Name:	MAN4
Ordered Locus Names:	Os03g0828500, LOC_Os03g61280
ORF Names:	OSJNBa0010E04.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Tissue specificity	Ubiquitous. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q10B67-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q10B67-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Mannan endo-1,4-beta-mannosidase 4		PRO_0000277485
Sites
	Active site	196	1	Proton donor By similarity
	Active site	314	1	Nucleophile By similarity
Natural variations
	Alternative sequence	1 – 22	22	Missing in isoform 2.		VSP_023021
Experimental info
	Sequence conflict	421	1	G → S in AK106915. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 872068A0D8DDD08F	FASTA	461	50,739
	10 20 30 40 50 60 MSSCALVQRP PPWRAAVAPG DGMVAVDGTQ FVVDCGRTIF FSGFNAYWLM MMAADPALRG 70 80 90 100 110 120 AVATAFQQAS AHGLNLARTW AFSDGGDQPL QSSPGVYNET MFQGLDFVIA EARRHGIYLL 130 140 150 160 170 180 LCLTNNFDNF GGKRQYVRWA GDAGHNLTSD DDFFTSTIVK SYFKNHVKTV LTRVNTLTGV 190 200 210 220 230 240 AYKDDPTIFA WELMNEPRCY ADPTGAMVQA WVEEMAPYVK SVDGRHLVTP GLEGFYGAGE 250 260 270 280 290 300 HESKELNPWG IYYGTNYVAT HRTAAVDFAT IHLYPDVWLW GSSADEQATF FRNWTRSHID 310 320 330 340 350 360 ATAAYLGMPL LVTEYGKFLW KEVGANKAQR NYFLDLVLDA IYASASRGGP LVGGAFWQLL 370 380 390 400 410 420 LDGDIVAGMD SLRDGYEIIL AEDSRAASII GEHSEQLAAL NGQDADVLCR RASSHRRTRL 430 440 450 460 GNSLSCGGGD TLELLLRMVL ACFVSLSRSI SSFIVQNFIL L
	Isoform 2. Checksum: 881013E73BC3D91D	FASTA	439	48,491
	10 20 30 40 50 60 MVAVDGTQFV VDCGRTIFFS GFNAYWLMMM AADPALRGAV ATAFQQASAH GLNLARTWAF 70 80 90 100 110 120 SDGGDQPLQS SPGVYNETMF QGLDFVIAEA RRHGIYLLLC LTNNFDNFGG KRQYVRWAGD 130 140 150 160 170 180 AGHNLTSDDD FFTSTIVKSY FKNHVKTVLT RVNTLTGVAY KDDPTIFAWE LMNEPRCYAD 190 200 210 220 230 240 PTGAMVQAWV EEMAPYVKSV DGRHLVTPGL EGFYGAGEHE SKELNPWGIY YGTNYVATHR 250 260 270 280 290 300 TAAVDFATIH LYPDVWLWGS SADEQATFFR NWTRSHIDAT AAYLGMPLLV TEYGKFLWKE 310 320 330 340 350 360 VGANKAQRNY FLDLVLDAIY ASASRGGPLV GGAFWQLLLD GDIVAGMDSL RDGYEIILAE 370 380 390 400 410 420 DSRAASIIGE HSEQLAALNG QDADVLCRRA SSHRRTRLGN SLSCGGGDTL ELLLRMVLAC 430 FVSLSRSISS FIVQNFILL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Nipponbare.
[5]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed: 16897088] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC096687 Genomic DNA. Translation: AAL79758.1. DP000009 Genomic DNA. Translation: ABF99676.1. DP000009 Genomic DNA. Translation: ABF99677.1. AP008209 Genomic DNA. Translation: BAF13691.1. AK106915 mRNA. No translation available.
RefSeq	NP_001051777.1.
UniGene	Os.7260
3D structure databases
HSSP	HSSP built from PDB template 1QNR based on UniProtKB Q99036.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Genome annotation databases
GeneID	4334653.
GenomeReviews	Gene locus MAN4 in contig AP008209_GR.
KEGG	osa:4334653.
NMPDR	fig|39947.1.peg.1972.
Organism-specific databases
Gramene	Q8SAY2.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN4_ORYSJ

Accession

Primary (citable) accession number: Q10B67
Secondary accession number(s): Q10B68, Q8SAY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	September 22, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)