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Protein

Mannan endo-1,4-beta-mannosidase 4

Gene

MAN4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei196 – 1961Proton donorBy similarity
Active sitei314 – 3141NucleophileBy similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 4 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 4
Endo-beta-1,4-mannanase 4
OsMAN4
Gene namesi
Name:MAN4
Ordered Locus Names:Os03g0828500, LOC_Os03g61280
ORF Names:OSJNBa0010E04.2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 3

Organism-specific databases

GrameneiQ10B67.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Mannan endo-1,4-beta-mannosidase 4PRO_0000277485Add
BLAST

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ10B67. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ10B67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3934.
HOGENOMiHOG000169952.
InParanoidiQ10B67.
OMAiFYGDGAH.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q10B67-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSCALVQRP PPWRAAVAPG DGMVAVDGTQ FVVDCGRTIF FSGFNAYWLM
60 70 80 90 100
MMAADPALRG AVATAFQQAS AHGLNLARTW AFSDGGDQPL QSSPGVYNET
110 120 130 140 150
MFQGLDFVIA EARRHGIYLL LCLTNNFDNF GGKRQYVRWA GDAGHNLTSD
160 170 180 190 200
DDFFTSTIVK SYFKNHVKTV LTRVNTLTGV AYKDDPTIFA WELMNEPRCY
210 220 230 240 250
ADPTGAMVQA WVEEMAPYVK SVDGRHLVTP GLEGFYGAGE HESKELNPWG
260 270 280 290 300
IYYGTNYVAT HRTAAVDFAT IHLYPDVWLW GSSADEQATF FRNWTRSHID
310 320 330 340 350
ATAAYLGMPL LVTEYGKFLW KEVGANKAQR NYFLDLVLDA IYASASRGGP
360 370 380 390 400
LVGGAFWQLL LDGDIVAGMD SLRDGYEIIL AEDSRAASII GEHSEQLAAL
410 420 430 440 450
NGQDADVLCR RASSHRRTRL GNSLSCGGGD TLELLLRMVL ACFVSLSRSI
460
SSFIVQNFIL L
Length:461
Mass (Da):50,739
Last modified:February 6, 2007 - v2
Checksum:i872068A0D8DDD08F
GO
Isoform 2 (identifier: Q10B67-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:439
Mass (Da):48,491
Checksum:i881013E73BC3D91D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti421 – 4211G → S in AK106915 (PubMed:12869764).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform 2. CuratedVSP_023021Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC096687 Genomic DNA. Translation: AAL79758.1.
DP000009 Genomic DNA. Translation: ABF99676.1.
DP000009 Genomic DNA. Translation: ABF99677.1.
AP008209 Genomic DNA. Translation: BAF13691.1.
AK106915 mRNA. No translation available.
RefSeqiNP_001051777.1. NM_001058312.1. [Q10B67-1]
UniGeneiOs.7260.

Genome annotation databases

EnsemblPlantsiOS03T0828500-01; OS03T0828500-01; OS03G0828500. [Q10B67-1]
GeneIDi4334653.
KEGGiosa:4334653.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC096687 Genomic DNA. Translation: AAL79758.1.
DP000009 Genomic DNA. Translation: ABF99676.1.
DP000009 Genomic DNA. Translation: ABF99677.1.
AP008209 Genomic DNA. Translation: BAF13691.1.
AK106915 mRNA. No translation available.
RefSeqiNP_001051777.1. NM_001058312.1. [Q10B67-1]
UniGeneiOs.7260.

3D structure databases

ProteinModelPortaliQ10B67.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0828500-01; OS03T0828500-01; OS03G0828500. [Q10B67-1]
GeneIDi4334653.
KEGGiosa:4334653.

Organism-specific databases

GrameneiQ10B67.

Phylogenomic databases

eggNOGiCOG3934.
HOGENOMiHOG000169952.
InParanoidiQ10B67.
OMAiFYGDGAH.

Gene expression databases

ExpressionAtlasiQ10B67. baseline.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Nipponbare.
  5. "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
    Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
    Funct. Integr. Genomics 7:1-16(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAN4_ORYSJ
AccessioniPrimary (citable) accession number: Q10B67
Secondary accession number(s): Q10B68, Q8SAY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: January 7, 2015
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.