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Q10B67

- MAN4_ORYSJ

UniProt

Q10B67 - MAN4_ORYSJ

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Protein
Mannan endo-1,4-beta-mannosidase 4
Gene
MAN4, Os03g0828500, LOC_Os03g61280, OSJNBa0010E04.2
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei196 – 1961Proton donor By similarity
Active sitei314 – 3141Nucleophile By similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 4 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 4
Endo-beta-1,4-mannanase 4
OsMAN4
Gene namesi
Name:MAN4
Ordered Locus Names:Os03g0828500, LOC_Os03g61280
ORF Names:OSJNBa0010E04.2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ10B67.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Mannan endo-1,4-beta-mannosidase 4
PRO_0000277485Add
BLAST

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ10B67.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3934.
HOGENOMiHOG000169952.
OMAiGAMVQAW.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q10B67-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSCALVQRP PPWRAAVAPG DGMVAVDGTQ FVVDCGRTIF FSGFNAYWLM    50
MMAADPALRG AVATAFQQAS AHGLNLARTW AFSDGGDQPL QSSPGVYNET 100
MFQGLDFVIA EARRHGIYLL LCLTNNFDNF GGKRQYVRWA GDAGHNLTSD 150
DDFFTSTIVK SYFKNHVKTV LTRVNTLTGV AYKDDPTIFA WELMNEPRCY 200
ADPTGAMVQA WVEEMAPYVK SVDGRHLVTP GLEGFYGAGE HESKELNPWG 250
IYYGTNYVAT HRTAAVDFAT IHLYPDVWLW GSSADEQATF FRNWTRSHID 300
ATAAYLGMPL LVTEYGKFLW KEVGANKAQR NYFLDLVLDA IYASASRGGP 350
LVGGAFWQLL LDGDIVAGMD SLRDGYEIIL AEDSRAASII GEHSEQLAAL 400
NGQDADVLCR RASSHRRTRL GNSLSCGGGD TLELLLRMVL ACFVSLSRSI 450
SSFIVQNFIL L 461
Length:461
Mass (Da):50,739
Last modified:February 6, 2007 - v2
Checksum:i872068A0D8DDD08F
GO
Isoform 2 (identifier: Q10B67-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:439
Mass (Da):48,491
Checksum:i881013E73BC3D91D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform 2.
VSP_023021Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti421 – 4211G → S in AK106915. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC096687 Genomic DNA. Translation: AAL79758.1.
DP000009 Genomic DNA. Translation: ABF99676.1.
DP000009 Genomic DNA. Translation: ABF99677.1.
AP008209 Genomic DNA. Translation: BAF13691.1.
AK106915 mRNA. No translation available.
RefSeqiNP_001051777.1. NM_001058312.1. [Q10B67-1]
UniGeneiOs.7260.

Genome annotation databases

EnsemblPlantsiOS03T0828500-01; OS03T0828500-01; OS03G0828500. [Q10B67-1]
GeneIDi4334653.
KEGGiosa:4334653.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC096687 Genomic DNA. Translation: AAL79758.1 .
DP000009 Genomic DNA. Translation: ABF99676.1 .
DP000009 Genomic DNA. Translation: ABF99677.1 .
AP008209 Genomic DNA. Translation: BAF13691.1 .
AK106915 mRNA. No translation available.
RefSeqi NP_001051777.1. NM_001058312.1. [Q10B67-1 ]
UniGenei Os.7260.

3D structure databases

ProteinModelPortali Q10B67.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0828500-01 ; OS03T0828500-01 ; OS03G0828500 . [Q10B67-1 ]
GeneIDi 4334653.
KEGGi osa:4334653.

Organism-specific databases

Gramenei Q10B67.

Phylogenomic databases

eggNOGi COG3934.
HOGENOMi HOG000169952.
OMAi GAMVQAW.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Nipponbare.
  5. "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
    Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
    Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAN4_ORYSJ
AccessioniPrimary (citable) accession number: Q10B67
Secondary accession number(s): Q10B68, Q8SAY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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