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Q10B67

- MAN4_ORYSJ

UniProt

Q10B67 - MAN4_ORYSJ

Protein

Mannan endo-1,4-beta-mannosidase 4

Gene

MAN4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei196 – 1961Proton donorBy similarity
    Active sitei314 – 3141NucleophileBy similarity

    GO - Molecular functioni

    1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase 4 (EC:3.2.1.78)
    Alternative name(s):
    Beta-mannanase 4
    Endo-beta-1,4-mannanase 4
    OsMAN4
    Gene namesi
    Name:MAN4
    Ordered Locus Names:Os03g0828500, LOC_Os03g61280
    ORF Names:OSJNBa0010E04.2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ10B67.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Mannan endo-1,4-beta-mannosidase 4PRO_0000277485Add
    BLAST

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ10B67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3934.
    HOGENOMiHOG000169952.
    OMAiGAMVQAW.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q10B67-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSCALVQRP PPWRAAVAPG DGMVAVDGTQ FVVDCGRTIF FSGFNAYWLM    50
    MMAADPALRG AVATAFQQAS AHGLNLARTW AFSDGGDQPL QSSPGVYNET 100
    MFQGLDFVIA EARRHGIYLL LCLTNNFDNF GGKRQYVRWA GDAGHNLTSD 150
    DDFFTSTIVK SYFKNHVKTV LTRVNTLTGV AYKDDPTIFA WELMNEPRCY 200
    ADPTGAMVQA WVEEMAPYVK SVDGRHLVTP GLEGFYGAGE HESKELNPWG 250
    IYYGTNYVAT HRTAAVDFAT IHLYPDVWLW GSSADEQATF FRNWTRSHID 300
    ATAAYLGMPL LVTEYGKFLW KEVGANKAQR NYFLDLVLDA IYASASRGGP 350
    LVGGAFWQLL LDGDIVAGMD SLRDGYEIIL AEDSRAASII GEHSEQLAAL 400
    NGQDADVLCR RASSHRRTRL GNSLSCGGGD TLELLLRMVL ACFVSLSRSI 450
    SSFIVQNFIL L 461
    Length:461
    Mass (Da):50,739
    Last modified:February 6, 2007 - v2
    Checksum:i872068A0D8DDD08F
    GO
    Isoform 2 (identifier: Q10B67-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-22: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:439
    Mass (Da):48,491
    Checksum:i881013E73BC3D91D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti421 – 4211G → S in AK106915. (PubMed:12869764)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2222Missing in isoform 2. CuratedVSP_023021Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC096687 Genomic DNA. Translation: AAL79758.1.
    DP000009 Genomic DNA. Translation: ABF99676.1.
    DP000009 Genomic DNA. Translation: ABF99677.1.
    AP008209 Genomic DNA. Translation: BAF13691.1.
    AK106915 mRNA. No translation available.
    RefSeqiNP_001051777.1. NM_001058312.1. [Q10B67-1]
    UniGeneiOs.7260.

    Genome annotation databases

    EnsemblPlantsiOS03T0828500-01; OS03T0828500-01; OS03G0828500. [Q10B67-1]
    GeneIDi4334653.
    KEGGiosa:4334653.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC096687 Genomic DNA. Translation: AAL79758.1 .
    DP000009 Genomic DNA. Translation: ABF99676.1 .
    DP000009 Genomic DNA. Translation: ABF99677.1 .
    AP008209 Genomic DNA. Translation: BAF13691.1 .
    AK106915 mRNA. No translation available.
    RefSeqi NP_001051777.1. NM_001058312.1. [Q10B67-1 ]
    UniGenei Os.7260.

    3D structure databases

    ProteinModelPortali Q10B67.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0828500-01 ; OS03T0828500-01 ; OS03G0828500 . [Q10B67-1 ]
    GeneIDi 4334653.
    KEGGi osa:4334653.

    Organism-specific databases

    Gramenei Q10B67.

    Phylogenomic databases

    eggNOGi COG3934.
    HOGENOMi HOG000169952.
    OMAi GAMVQAW.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Nipponbare.
    5. "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
      Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
      Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMAN4_ORYSJ
    AccessioniPrimary (citable) accession number: Q10B67
    Secondary accession number(s): Q10B68, Q8SAY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3