Reviewed, UniProtKB/Swiss-Prot Q10991 (CATL1_SHEEP)

Names and origin

Protein names

Recommended name:
    Cathepsin L1
    EC=3.4.22.15
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin L1 heavy chain
    2- Recommended name:
            Cathepsin L1 light chain

Gene names

Name:	CTSL
Synonyms:	CTSL1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes.
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Cathepsin L1 heavy chain		PRO_0000026260
	Propeptide	176	1			PRO_0000026261
	Chain	177 – 217	41	Cathepsin L1 light chain		PRO_0000026262
Sites
	Active site	25	1	By similarity
	Active site	163	1	By similarity
	Active site	184	1	By similarity
Amino acid modifications
	Disulfide bond	22 ↔ 65		By similarity
	Disulfide bond	156 ↔ 206		Interchain (between heavy and light chains) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10991-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 552F6ED563564406	FASTA	217	23,608
	10 20 30 40 50 60 VPKSVDWTKK GYVTPVKNQG QCGSCWAFSA TGALEGQMFR KTGKLVSLSE QNLVDSSRPQ 70 80 90 100 110 120 GNQGCNGGLM DNAFQYIKEN GGLDSEESYP YEATDTSCNY KPEYSAAKDT GFVDIPQREK 130 140 150 160 170 180 ALMKAVATVG PISVAIDAGH SSFQFYKSGI YYDPDCSSKD LDHGVLVVGY GFEGTNNKFW 190 200 210 IVKNSWGPEW GNKGYVKMAK DQNNHCGIAT AASYPTV

References

[1]	"The amino acid sequences, structure comparisons and inhibition kinetics of sheep cathepsin L and sheep stefin B." Ritonja A., Coetzer T.H.T., Pike R.N., Dennison C. Comp. Biochem. Physiol. 114B:193-198(1996) [PubMed: 8759291] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1FH0 based on UniProtKB O60911.
SMR	Q10991. Positions 2-217.
ModBase	Search...
Protein family/group databases
MEROPS	C01.032.
Phylogenomic databases
HOVERGEN	Q10991.
Enzyme and pathway databases
BRENDA	3.4.22.15. 271.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL1_SHEEP

Accession

Primary (citable) accession number: Q10991

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)