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Protein

Galactoside 2-alpha-L-fucosyltransferase 2

Gene

Fut2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transfer of fucose to the terminal galactose on glycan chains of cell surface glycoproteins and glycolipids (PubMed:11341836, PubMed:11179967). The resulting epitope plays a role in cell-cell interaction including host-microbe interaction (By similarity). Mediates interaction with intestinal microbiota influencing its composition (By similarity). Creates a membrane-associated precursor oligosaccharide Fuc-alpha((1,2)Gal-beta-) called the H antigen which is an essential substrate for the final step in the membrane-associated blood group antigen synthesis pathway (PubMed:11179967).By similarity2 Publications

Catalytic activityi

GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.2 Publications

Kineticsi

  1. KM=33 mM for phenyl-beta-galactose1 Publication
  2. KM=20 mM for N-acetyllactosamine1 Publication
  3. KM=8.3 mM for lacto-N-biose1 Publication
  4. KM=3.8 mM for galacto-N-biose1 Publication
  5. KM=24 µM for GDP-fucose1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 Publications
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    • galactoside 2-alpha-L-fucosyltransferase activity Source: RGD

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.69. 5301.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT11. Glycosyltransferase Family 11.

    Chemistry databases

    SwissLipidsiSLP:000000796.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 2 (EC:2.4.1.692 Publications)
    Alternative name(s):
    Alpha 1,2-fucosyltransferase
    Alpha 1,2-fucosyltransferase B
    Alpha 1-2 fucosyltransferase
    Alpha(1,2)FT 2
    Fucosyltransferase 2
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
    Secretor blood group alpha-2-fucosyltransferase
    Gene namesi
    Name:Fut2
    Synonyms:Ftb, Sec1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Unplaced

    Organism-specific databases

    RGDi2639. Fut2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 5CytoplasmicSequence analysis5
    Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini27 – 354LumenalSequence analysisAdd BLAST328

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001491141 – 354Galactoside 2-alpha-L-fucosyltransferase 2Add BLAST354

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi199N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ10984.
    PRIDEiQ10984.

    Expressioni

    Tissue specificityi

    Specifically expressed in gut.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000021011.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028519.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 11 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFKR. Eukaryota.
    ENOG4111HG3. LUCA.
    HOGENOMiHOG000261621.
    HOVERGENiHBG004338.
    InParanoidiQ10984.
    KOiK00718.
    PhylomeDBiQ10984.
    TreeFamiTF315810.

    Family and domain databases

    InterProiIPR002516. Glyco_trans_11.
    [Graphical view]
    PANTHERiPTHR11927. PTHR11927. 1 hit.
    PfamiPF01531. Glyco_transf_11. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q10984-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MASAQVPFSF PLAHFLIFVF VTSTITHLQQ RIVKLQPLSE KELPMTTQMS
    60 70 80 90 100
    SGNTESPEMR RDSEQHGNGE LRGMFTINSI GRLGNQMGEY ATLFALARMN
    110 120 130 140 150
    GRLAFIPASM HNALAPIFRI SLPVLHSDTA KKIPWQNYHL NDWMEERYRH
    160 170 180 190 200
    IPGHFVRFTG YPCSWTFYHH LRPEILKEFT LHDHVREEAQ AFLRGLRVNG
    210 220 230 240 250
    SQPSTFVGVH VRRGDYVHVM PNVWKGVVAD RGYLEKALDM FRARYSSPVF
    260 270 280 290 300
    VVTSNGMAWC RENINASRGD VVFAGNGIEG SPAKDFALLT QCNHTIMTIG
    310 320 330 340 350
    TFGIWAAYLA GGDTIYLANY TLPDSPFLKV FKPEAAFLPE WVGIPADLSP

    LLKH
    Length:354
    Mass (Da):39,983
    Last modified:December 6, 2005 - v2
    Checksum:i123E8C8379E8559E
    GO
    Isoform 2 (identifier: Q10984-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         354-354: H → ALTPACPRSHFHLKAKGVTCYVAGRAF

    Show »
    Length:380
    Mass (Da):42,730
    Checksum:i8183786AE8212EBE
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti26T → I (PubMed:11341836).Curated1
    Sequence conflicti26T → I (Ref. 3) Curated1
    Sequence conflicti271V → G in AAB41515 (PubMed:8010942).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_016526354H → ALTPACPRSHFHLKAKGVTC YVAGRAF in isoform 2. 2 Publications1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF264005 mRNA. Translation: AAF72200.1.
    AF131238 Genomic DNA. Translation: AAD24469.1.
    AB006138 mRNA. Translation: BAA21742.1.
    L26010 mRNA. Translation: AAB41515.1.
    PIRiS46494.
    RefSeqiNP_113823.1. NM_031635.1.
    XP_006229197.1. XM_006229135.3.
    UniGeneiRn.10678.

    Genome annotation databases

    GeneIDi58924.
    KEGGirno:58924.
    UCSCiRGD:2639. rat. [Q10984-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF264005 mRNA. Translation: AAF72200.1.
    AF131238 Genomic DNA. Translation: AAD24469.1.
    AB006138 mRNA. Translation: BAA21742.1.
    L26010 mRNA. Translation: AAB41515.1.
    PIRiS46494.
    RefSeqiNP_113823.1. NM_031635.1.
    XP_006229197.1. XM_006229135.3.
    UniGeneiRn.10678.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028519.

    Chemistry databases

    SwissLipidsiSLP:000000796.

    Protein family/group databases

    CAZyiGT11. Glycosyltransferase Family 11.

    Proteomic databases

    PaxDbiQ10984.
    PRIDEiQ10984.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi58924.
    KEGGirno:58924.
    UCSCiRGD:2639. rat. [Q10984-1]

    Organism-specific databases

    CTDi2524.
    RGDi2639. Fut2.

    Phylogenomic databases

    eggNOGiENOG410IFKR. Eukaryota.
    ENOG4111HG3. LUCA.
    HOGENOMiHOG000261621.
    HOVERGENiHBG004338.
    InParanoidiQ10984.
    KOiK00718.
    PhylomeDBiQ10984.
    TreeFamiTF315810.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    BRENDAi2.4.1.69. 5301.

    Miscellaneous databases

    PROiQ10984.

    Gene expression databases

    BgeeiENSRNOG00000021011.

    Family and domain databases

    InterProiIPR002516. Glyco_trans_11.
    [Graphical view]
    PANTHERiPTHR11927. PTHR11927. 1 hit.
    PfamiPF01531. Glyco_transf_11. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFUT2_RAT
    AccessioniPrimary (citable) accession number: Q10984
    Secondary accession number(s): O35087, Q9JK44, Q9R275
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 6, 2005
    Last modified: November 30, 2016
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In rat, there are three genes (Fut1/Fta, Fut2/Ftb and Ftc) which encode galactoside 2-L-fucosyltransferase. They are expressed in a tissue-specific manner and Ftc may have no enzymatic activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.