Q10984 (FUT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactoside 2-alpha-L-fucosyltransferase 2
EC=2.4.1.69

Alternative name(s):
Alpha 1,2-fucosyltransferase
Alpha 1,2-fucosyltransferase B
Alpha 1-2 fucosyltransferase
Alpha(1,2)FT 2
Fucosyltransferase 2
GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
Secretor blood group alpha-2-fucosyltransferase

Gene names

Name:	Fut2
Synonyms:	Ftb, Sec1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Creates a membrane-associated precursor oligosaccharide Fuc-alpha((1,2)Gal-beta-) called the H antigen which is an essential substrate for the final step in the membrane-associated A and B antigen synthesis pathway. Ref.2
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Tissue specificity	Specifically expressed in gut. Ref.2
Miscellaneous	In rat, there are three genes (Fut1/Fta, Fut2/Ftb and Ftc) which encode galactoside 2-L-fucosyltransferase. They are expressed in a tissue-specific manner and Ftc may have no enzymatic activity.
Sequence similarities	Belongs to the glycosyltransferase 11 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from direct assay Ref.2. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q10984-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q10984-2)

The sequence of this isoform differs from the canonical sequence as follows:
     354-354: H → ALTPACPRSHFHLKAKGVTCYVAGRAF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Galactoside 2-alpha-L-fucosyltransferase 2		PRO_0000149114
Regions
	Topological domain	1 – 5	5	Cytoplasmic Potential
	Transmembrane	6 – 26	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	27 – 354	328	Lumenal Potential
Amino acid modifications
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	354	1	H → ALTPACPRSHFHLKAKGVTC YVAGRAF in isoform 2.		VSP_016526
Experimental info
	Sequence conflict	26	1	T → I Ref.1
	Sequence conflict	26	1	T → I Ref.3
	Sequence conflict	271	1	V → G in AAB41515. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 123E8C8379E8559E	FASTA	354	39,983
	10 20 30 40 50 60 MASAQVPFSF PLAHFLIFVF VTSTITHLQQ RIVKLQPLSE KELPMTTQMS SGNTESPEMR 70 80 90 100 110 120 RDSEQHGNGE LRGMFTINSI GRLGNQMGEY ATLFALARMN GRLAFIPASM HNALAPIFRI 130 140 150 160 170 180 SLPVLHSDTA KKIPWQNYHL NDWMEERYRH IPGHFVRFTG YPCSWTFYHH LRPEILKEFT 190 200 210 220 230 240 LHDHVREEAQ AFLRGLRVNG SQPSTFVGVH VRRGDYVHVM PNVWKGVVAD RGYLEKALDM 250 260 270 280 290 300 FRARYSSPVF VVTSNGMAWC RENINASRGD VVFAGNGIEG SPAKDFALLT QCNHTIMTIG 310 320 330 340 350 TFGIWAAYLA GGDTIYLANY TLPDSPFLKV FKPEAAFLPE WVGIPADLSP LLKH
	Isoform 2 [UniParc]. Checksum: 8183786AE8212EBE	FASTA	380	42,730
	10 20 30 40 50 60 MASAQVPFSF PLAHFLIFVF VTSTITHLQQ RIVKLQPLSE KELPMTTQMS SGNTESPEMR 70 80 90 100 110 120 RDSEQHGNGE LRGMFTINSI GRLGNQMGEY ATLFALARMN GRLAFIPASM HNALAPIFRI 130 140 150 160 170 180 SLPVLHSDTA KKIPWQNYHL NDWMEERYRH IPGHFVRFTG YPCSWTFYHH LRPEILKEFT 190 200 210 220 230 240 LHDHVREEAQ AFLRGLRVNG SQPSTFVGVH VRRGDYVHVM PNVWKGVVAD RGYLEKALDM 250 260 270 280 290 300 FRARYSSPVF VVTSNGMAWC RENINASRGD VVFAGNGIEG SPAKDFALLT QCNHTIMTIG 310 320 330 340 350 360 TFGIWAAYLA GGDTIYLANY TLPDSPFLKV FKPEAAFLPE WVGIPADLSP LLKALTPACP 370 380 RSHFHLKAKG VTCYVAGRAF

References

[1]	"An amino acid region at the N-terminus of rat hepatoma alpha1-->2 fucosyltransferase modulates enzyme activity and interaction with lipids: strong preference for glycosphingolipids containing terminal Galbeta1-->3GalNAc-structures." Sherwood A.L., Stroud M.R., Levery S.B., Holmes E.H. Biochemistry 40:5708-5719(2001) [PubMed: 11341836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Fischer.
[2]	"Comparison of the three rat GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferases FTA, FTB and FTC." Bureau V., Marionneau S., Cailleau-Thomas A., Le Moullac-Vaidye B., Liehr T., Le Pendu J. Eur. J. Biochem. 268:1006-1019(2001) [PubMed: 11179967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY. Strain: BDIX.
[3]	Soejima M., Wang B., Koda Y., Kimura H. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Colon cancer.
[4]	"Evidence for two distinct alpha(1,2)-fucosyltransferase genes differentially expressed throughout the rat colon." Piau J.-P., Labarriere N., Dabouis G., Denis M.G. Biochem. J. 300:623-626(1994) [PubMed: 8010942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 222-354 (ISOFORM 2). Strain: BDIX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF264005 mRNA. Translation: AAF72200.1. AF131238 Genomic DNA. Translation: AAD24469.1. AB006138 mRNA. Translation: BAA21742.1. L26010 mRNA. Translation: AAB41515.1.
IPI	IPI00202052. IPI00559563.
PIR	S46494.
RefSeq	NP_113823.1. NM_031635.1.
UniGene	Rn.10678.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q10984.
Protein family/group databases
CAZy	GT11. Glycosyltransferase Family 11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028519; ENSRNOP00000028519; ENSRNOG00000021011.
GeneID	58924.
KEGG	rno:58924.
UCSC	NM_031635. rat.
Organism-specific databases
CTD	2524.
RGD	2639. Fut2.
Phylogenomic databases
eggNOG	maNOG09331.
GeneTree	ENSGT00390000001450.
HOVERGEN	HBG004338.
InParanoid	Q10984.
OrthoDB	EOG4BK545.
Gene expression databases
ArrayExpress	Q10984.
Genevestigator	Q10984.
GermOnline	ENSRNOG00000021011. Rattus norvegicus.
Family and domain databases
InterPro	IPR002516. Glyco_trans_11. [Graphical view]
KO	K00718.
PANTHER	PTHR11927. Glyco_trans_11. 1 hit.
Pfam	PF01531. Glyco_transf_11. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611490.

Entry information

Entry name

FUT2_RAT

Accession

Primary (citable) accession number: Q10984
Secondary accession number(s): O35087, Q9JK44, Q9R275

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 6, 2005
Last modified:	November 16, 2011
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program