Q10983 (FUT2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactoside 2-alpha-L-fucosyltransferase 2
EC=2.4.1.69

Alternative name(s):
Alpha(1,2)FT 2
Fucosyltransferase 2
GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
Secretor blood group alpha-2-fucosyltransferase

Gene names

Name:	FUT2
Synonyms:	RFT-II, SEC1

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values.
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Tissue specificity	Salivary and lactating mammary glands.
Miscellaneous	There are three genes (Fut1, Fut2 and Fut3) which encode galactoside 2-L-fucosyltransferase in rabbit. They are expressed in a tissue-specific manner.
Sequence similarities	Belongs to the glycosyltransferase 11 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Galactoside 2-alpha-L-fucosyltransferase 2		PRO_0000149113
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 43	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	44 – 354	311	Lumenal Potential
Amino acid modifications
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	317	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10983 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1E2B831F9DA6CCB4	FASTA	354	40,035
	10 20 30 40 50 60 MRFAPDYVLC PPTATRRLRA THPSVSTIYF LFTIFVVSTV FHCHQRLALV PAPWAYSARV 70 80 90 100 110 120 VVVPGHLPRE GMWTINAMGR LGNQMGEYAT LYALAKENGR PAYIPAQMHS TLAPIFRISL 130 140 150 160 170 180 PVLHSSTASR VPWQNYHLND WMEERYRHIP VPYVRLTGYP CSWTFYHHLR HEILREFTLH 190 200 210 220 230 240 DHVREEAQAF LRGLRVNGSR PSTFVGVHVR RGDYVRVMPQ VWKGVVADRG YLEQALDWFR 250 260 270 280 290 300 APTAPPVFVV TSNGMAWCRE NIDASRGDVV FAGNGLEGSP AKDFALLTQC NHTVMTIGTF 310 320 330 340 350 GFWAAYLTGG DTVYLANYTA PDSPFHLVFK PEAAFLPEWV GITANMGRAL WSGL

References

[1]	"Molecular cloning and expression of two types of rabbit beta-galactoside alpha 1,2-fucosyltransferase." Hitoshi S., Kusunoki S., Kanazawa I., Tsuji S. J. Biol. Chem. 270:8844-8850(1995) [PubMed: 7721792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80225 mRNA. Translation: CAA56512.1.
PIR	B56392.
RefSeq	NP_001075871.1. NM_001082402.1.
UniGene	Ocu.2107.
3D structure databases
ProteinModelPortal	Q10983.
ModBase	Search...
Protein-protein interaction databases
STRING	Q10983.
Protein family/group databases
CAZy	GT11. Glycosyltransferase Family 11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009290.
Organism-specific databases
CTD	100009290.
Phylogenomic databases
HOVERGEN	HBG004338.
Family and domain databases
InterPro	IPR002516. Glyco_trans_11. [Graphical view]
PANTHER	PTHR11927. Glyco_trans_11. 1 hit.
Pfam	PF01531. Glyco_transf_11. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT2_RABIT

Accession

Primary (citable) accession number: Q10983

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 19, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program