Reviewed, UniProtKB/Swiss-Prot Q10982 (FUT2_PIG)

Names and origin

Protein names

Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 2
    EC=2.4.1.69
Alternative name(s):
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
    Alpha(1,2)FT 2
    Fucosyltransferase 2

Gene names

Name:

FUT2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Galactoside 2-alpha-L-fucosyltransferase 2		PRO_0000149111
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 340	312	Lumenal Potential
Amino acid modifications
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	158	1	R → H in CAA67932. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q10982-1 [UniParc]. Last modified July 15, 1999. Version 3. Checksum: 0629F1C04FC206AD	FASTA	340	38,987
	10 20 30 40 50 60 MLSMQASFFF PTGPFILFVF TASTIFHLQQ RMVKIQPTWE LQMVTQVTTE SPSSPQLKGM 70 80 90 100 110 120 WTINAIGRLG NQMGEYATLY ALARMNGRPA FIPPEMHSTL APIFRITLPV LHASTARRIP 130 140 150 160 170 180 WQNYHLNDWM EERYRHIPGE YVRLTGYPCS WTFYHHLRTE ILREFTLHNH VREEAQDFLR 190 200 210 220 230 240 GLRVNGSRPS TYVGVHVRRG DYVHVMPNVW KGVVADRRYL EQALDWFRAR YRSPVFVVSS 250 260 270 280 290 300 NGMAWCRENI NASRGDVVFA GNGIEGSPAK DFALLTQCNH TVMTIGTFGI WAAYLAGGET 310 320 330 340 IYLANYTLPD SPFLKLFKPE AAFLPEWIGI EADLSPLLKH

References

[1]	"Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci." Meijerink E., Fries R., Voegeli P., Masabanda J., Wigger G., Stricker C., Neuenschwander S., Bertschinger H.U., Stranzinger G. Mamm. Genome 8:736-741(1997) [PubMed: 9321466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and characterization of the pig secretor type alpha(1,2)fucosyltransferase." Cohney S., Mouhtouris E., McKenzie I.F.C., Sandrin M.S. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"A DNA polymorphism influencing alpha(1,2)fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion." Meijerink E., Neuenschwander S., Fries R., Dinter A., Bertschinger H.U., Stranzinger G., Vogeli P. Immunogenetics 52:129-136(2000) [PubMed: 11132149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Small intestine.
[4]	Petit J.-M. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 69-334.
[5]	"Porcine submaxillary gland GDP-L-fucose: beta-D-galactoside alpha-2-L-fucosyltransferase is likely a counterpart of the human Secretor gene-encoded blood group transferase." Thurin J., Blaszczyk-Thurin M. J. Biol. Chem. 270:26577-26580(1995) [PubMed: 7592879] [Abstract] Cited for: PROTEIN SEQUENCE OF 69-80; 119-133 AND 316-334. Tissue: Submandibular gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U70881 Genomic DNA. Translation: AAB81883.1. AF027304 Genomic DNA. Translation: AAC09170.1. AF136895 mRNA. Translation: AAF59832.1. X99621 Genomic DNA. Translation: CAA67932.1.
RefSeq	NP_999234.1.
UniGene	Ssc.243
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT11. Glycosyltransferase Family 11.
Genome annotation databases
GeneID	397139.
KEGG	ssc:397139.
Organism-specific databases
CTD	397139.
Phylogenomic databases
HOVERGEN	Q10982.
Enzyme and pathway databases
BRENDA	2.4.1.69. 249.
Family and domain databases
InterPro	IPR002516. Glyco_trans_11. [Graphical view]
PANTHER	PTHR11927. Glyco_trans_11. 1 hit.
Pfam	PF01531. Glyco_transf_11. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT2_PIG

Accession

Primary (citable) accession number: Q10982
Secondary accession number(s): O19100, Q29044

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1999
Last modified:	October 13, 2009
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)