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Protein

Galactoside 2-alpha-L-fucosyltransferase 2

Gene

FUT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transfer of fucose to the terminal galactose on glycan chains of cell surface glycoproteins and glycolipids (PubMed:7876235). The resulting epitope plays a role in cell-cell interaction including host-microbe interaction (PubMed:12692541, PubMed:8018146). Mediates interaction with intestinal microbiota influencing its composition (PubMed:21625510, PubMed:24733310, PubMed:22068912). Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble ABO blood group antigen synthesis pathway (PubMed:7876235).5 Publications

Catalytic activityi

GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Kineticsi

  1. KM=11.5 mM for phenyl-beta-D-galactoside1 Publication
  2. KM=3.6 mM for lacto-N-biose1 Publication
  3. KM=3.8 mM for N-acetyllactosamine1 Publication
  4. KM=197 µM for GDP-fucose1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    • fucosyltransferase activity Source: ProtInc
    • galactoside 2-alpha-L-fucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    • carbohydrate metabolic process Source: ProtInc
    • L-fucose catabolic process Source: UniProtKB
    • protein glycosylation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Blood group antigen, Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciZFISH:HS11104-MONOMER.
    BRENDAi2.4.1.69. 2681.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT11. Glycosyltransferase Family 11.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 2 (EC:2.4.1.691 Publication)
    Alternative name(s):
    Alpha(1,2)FT 2
    Fucosyltransferase 2
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
    SE2
    Secretor blood group alpha-2-fucosyltransferase
    Secretor factor
    Short name:
    Se
    Gene namesi
    Name:FUT2
    Synonyms:SEC2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:4013. FUT2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
    Transmembranei15 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST14
    Topological domaini29 – 343LumenalSequence analysisAdd BLAST315

    GO - Cellular componenti

    • extracellular exosome Source: UniProtKB
    • Golgi apparatus Source: UniProtKB
    • Golgi cisterna membrane Source: UniProtKB-SubCell
    • integral component of membrane Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi2524.
    MIMi182100. gene+phenotype.
    612542. phenotype.
    OpenTargetsiENSG00000176920.
    PharmGKBiPA28429.

    Polymorphism and mutation databases

    BioMutaiFUT2.
    DMDMi1730125.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000121401 – 343Galactoside 2-alpha-L-fucosyltransferase 2Add BLAST343

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi282N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi308N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ10981.
    PeptideAtlasiQ10981.
    PRIDEiQ10981.

    PTM databases

    iPTMnetiQ10981.
    PhosphoSitePlusiQ10981.

    Expressioni

    Tissue specificityi

    Small intestine, colon and lung.

    Gene expression databases

    BgeeiENSG00000176920.
    CleanExiHS_FUT2.
    ExpressionAtlasiQ10981. baseline and differential.
    GenevisibleiQ10981. HS.

    Organism-specific databases

    HPAiHPA014402.

    Interactioni

    Protein-protein interaction databases

    BioGridi108800. 1 interactor.
    IntActiQ10981. 1 interactor.
    STRINGi9606.ENSP00000375748.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10981.
    SMRiQ10981.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 11 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFKR. Eukaryota.
    ENOG4111HG3. LUCA.
    GeneTreeiENSGT00390000001450.
    HOGENOMiHOG000261621.
    HOVERGENiHBG004338.
    InParanoidiQ10981.
    KOiK00718.
    OMAiAFLPEWT.
    OrthoDBiEOG091G0X2P.
    PhylomeDBiQ10981.
    TreeFamiTF315810.

    Family and domain databases

    InterProiIPR002516. Glyco_trans_11.
    [Graphical view]
    PANTHERiPTHR11927. PTHR11927. 1 hit.
    PfamiPF01531. Glyco_transf_11. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

    Isoform Long (identifier: Q10981-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MLVVQMPFSF PMAHFILFVF TVSTIFHVQQ RLAKIQAMWE LPVQIPVLAS
    60 70 80 90 100
    TSKALGPSQL RGMWTINAIG RLGNQMGEYA TLYALAKMNG RPAFIPAQMH
    110 120 130 140 150
    STLAPIFRIT LPVLHSATAS RIPWQNYHLN DWMEEEYRHI PGEYVRFTGY
    160 170 180 190 200
    PCSWTFYHHL RQEILQEFTL HDHVREEAQK FLRGLQVNGS RPGTFVGVHV
    210 220 230 240 250
    RRGDYVHVMP KVWKGVVADR RYLQQALDWF RARYSSLIFV VTSNGMAWCR
    260 270 280 290 300
    ENIDTSHGDV VFAGDGIEGS PAKDFALLTQ CNHTIMTIGT FGIWAAYLTG
    310 320 330 340
    GDTIYLANYT LPDSPFLKIF KPEAAFLPEW TGIAADLSPL LKH
    Length:343
    Mass (Da):39,017
    Last modified:October 1, 1996 - v1
    Checksum:i12066D9CF175E13A
    GO
    Isoform Short (identifier: Q10981-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-11: Missing.

    Show »
    Length:332
    Mass (Da):37,740
    Checksum:i96DE97ED89807A8A
    GO

    Sequence cautioni

    The sequence BAA11638 differs from that shown. Reason: Erroneous initiation.Curated

    Polymorphismi

    Three alleles have been identified in the Japanese population: Se1, Se2, and Sej.1 Publication
    Common polymorphisms in FUT2 define the vitamin B12 plasma level quantitative trait locus 1 (B12QTL1) [MIMi:612542]. Vitamin B12 found in meat and milk products is necessary for the formation of red blood cells, DNA synthesis during cell division, and maintenance of the myelin nerve sheath, among other functions. Deficiency in vitamin B12, clinically associated with pernicious anemia, cardiovascular disease, cancer, and neurodegenerative disorders, is often related to poor intestinal B12 absorption rather than direct dietary deficiency.1 Publication
    Genetic variation in FUT2 results in the non-secretor phenotype which gives rise to non-functional FUT2, resulting in a lack of the H type-1 oligosaccharide ligand in secretions, and this prevents Norwalk virus binding contributing to resistance to Norwalk virus infection.1 Publication

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_00342225I → V Polymorphism found in Xhosa population. 2 PublicationsCorresponds to variant rs1800021dbSNPEnsembl.1
    Natural variantiVAR_003423138R → C Polymorphism found in Xhosa population. 2 PublicationsCorresponds to variant rs1800022dbSNPEnsembl.1
    Natural variantiVAR_003424140I → F in allele Sej; non-secretor phenotype. 3 PublicationsCorresponds to variant rs1047781dbSNPEnsembl.1
    Natural variantiVAR_003425172D → N Polymorphism found in Xhosa population. 2 PublicationsCorresponds to variant rs1800025dbSNPEnsembl.1
    Natural variantiVAR_022187258G → S.1 PublicationCorresponds to variant rs602662dbSNPEnsembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0187361 – 11Missing in isoform Short. CuratedAdd BLAST11

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U17894 Genomic DNA. Translation: AAC24453.1.
    D89327 Genomic DNA. Translation: BAA13944.1.
    D87942 mRNA. Translation: BAA21684.1.
    AY937240 Genomic DNA. Translation: AAX14047.1.
    BC121066 mRNA. No translation available.
    D82933 Genomic DNA. Translation: BAA11638.1. Different initiation.
    CCDSiCCDS33069.1. [Q10981-1]
    PIRiA56098.
    RefSeqiNP_000502.4. NM_000511.5. [Q10981-1]
    NP_001091107.1. NM_001097638.2. [Q10981-1]
    UniGeneiHs.579928.

    Genome annotation databases

    EnsembliENST00000391876; ENSP00000375748; ENSG00000176920. [Q10981-1]
    ENST00000425340; ENSP00000387498; ENSG00000176920. [Q10981-1]
    GeneIDi2524.
    KEGGihsa:2524.
    UCSCiuc010emc.4. human. [Q10981-1]

    Keywords - Coding sequence diversityi

    Alternative initiation, Polymorphism

    Cross-referencesi

    Web resourcesi

    dbRBC/BGMUT

    Blood group antigen gene mutation database

    SeattleSNPs
    Functional Glycomics Gateway - GTase

    Fucosyltransferase 2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U17894 Genomic DNA. Translation: AAC24453.1.
    D89327 Genomic DNA. Translation: BAA13944.1.
    D87942 mRNA. Translation: BAA21684.1.
    AY937240 Genomic DNA. Translation: AAX14047.1.
    BC121066 mRNA. No translation available.
    D82933 Genomic DNA. Translation: BAA11638.1. Different initiation.
    CCDSiCCDS33069.1. [Q10981-1]
    PIRiA56098.
    RefSeqiNP_000502.4. NM_000511.5. [Q10981-1]
    NP_001091107.1. NM_001097638.2. [Q10981-1]
    UniGeneiHs.579928.

    3D structure databases

    ProteinModelPortaliQ10981.
    SMRiQ10981.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi108800. 1 interactor.
    IntActiQ10981. 1 interactor.
    STRINGi9606.ENSP00000375748.

    Protein family/group databases

    CAZyiGT11. Glycosyltransferase Family 11.

    PTM databases

    iPTMnetiQ10981.
    PhosphoSitePlusiQ10981.

    Polymorphism and mutation databases

    BioMutaiFUT2.
    DMDMi1730125.

    Proteomic databases

    PaxDbiQ10981.
    PeptideAtlasiQ10981.
    PRIDEiQ10981.

    Protocols and materials databases

    DNASUi2524.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000391876; ENSP00000375748; ENSG00000176920. [Q10981-1]
    ENST00000425340; ENSP00000387498; ENSG00000176920. [Q10981-1]
    GeneIDi2524.
    KEGGihsa:2524.
    UCSCiuc010emc.4. human. [Q10981-1]

    Organism-specific databases

    CTDi2524.
    DisGeNETi2524.
    GeneCardsiFUT2.
    HGNCiHGNC:4013. FUT2.
    HPAiHPA014402.
    MIMi182100. gene+phenotype.
    612542. phenotype.
    neXtProtiNX_Q10981.
    OpenTargetsiENSG00000176920.
    PharmGKBiPA28429.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiENOG410IFKR. Eukaryota.
    ENOG4111HG3. LUCA.
    GeneTreeiENSGT00390000001450.
    HOGENOMiHOG000261621.
    HOVERGENiHBG004338.
    InParanoidiQ10981.
    KOiK00718.
    OMAiAFLPEWT.
    OrthoDBiEOG091G0X2P.
    PhylomeDBiQ10981.
    TreeFamiTF315810.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    BioCyciZFISH:HS11104-MONOMER.
    BRENDAi2.4.1.69. 2681.

    Miscellaneous databases

    GeneWikiiFUT2.
    GenomeRNAii2524.
    PROiQ10981.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000176920.
    CleanExiHS_FUT2.
    ExpressionAtlasiQ10981. baseline and differential.
    GenevisibleiQ10981. HS.

    Family and domain databases

    InterProiIPR002516. Glyco_trans_11.
    [Graphical view]
    PANTHERiPTHR11927. PTHR11927. 1 hit.
    PfamiPF01531. Glyco_transf_11. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFUT2_HUMAN
    AccessioniPrimary (citable) accession number: Q10981
    Secondary accession number(s): Q0VAG5, Q14338, Q5D0G2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: November 2, 2016
    This is version 157 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    There are two genes (FUT1 and FUT2) which encode galactoside 2-L-fucosyltransferase. They are expressed in a tissue-specific manner with expression restricted to cells of mesodermal or endodermal origin respectively.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Blood group antigen proteins
      Nomenclature of blood group antigens and list of entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    7. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.