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Protein

ADP-ribosylation factor 1-like 2

Gene

arf-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Involved in endoplasmic reticulum dynamics during embryogenesis (PubMed:15716356). Also required for adult germline function (PubMed:15576487). Plays a role in cell shedding during embryogenesis probably by promoting the endocytosis of cell adhesion molecules (PubMed:22801495). During neurogenesis, involved in cell autonomous Q.p neuroblast asymmetric divisions that generate one precursor cell and one apoptotic cell, probably by controlling endocytosis (PubMed:21596567).1 Publication3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: UniProtKB
  • gamete generation Source: UniProtKB
  • intracellular protein transport Source: WormBase
  • mitochondrion organization Source: WormBase
  • oocyte development Source: WormBase
  • regulation of apoptotic process involved in development Source: UniProtKB
  • regulation of receptor-mediated endocytosis Source: WormBase
  • vesicle-mediated transport Source: UniProtKB-KW

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1660499 Synthesis of PIPs at the plasma membrane
R-CEL-1660514 Synthesis of PIPs at the Golgi membrane
R-CEL-421837 Clathrin derived vesicle budding
R-CEL-432722 Golgi Associated Vesicle Biogenesis
R-CEL-6807878 COPI-mediated anterograde transport
R-CEL-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-CEL-6811438 Intra-Golgi traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1-like 2
Alternative name(s):
ADP-ribosylation factor-related protein 1.2
Gene namesi
Name:arf-1.2
Synonyms:arf-1
ORF Names:B0336.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiB0336.2 ; CE00696 ; WBGene00000182 ; arf-1.2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Death during embryogenesis (PubMed:15716356). Some muscle differentiation occurs although it is poorly organized and little elongation or morphogenesis is evident (PubMed:15716356). Extra A/PVM neurons are produced from the precursor Q.pp, which is normally fated to die, acquiring the fate of its sister Q.pa (PubMed:21596567). Q.pa and Q.pp have almost similar size caused by a loss of Q.p asymmetric cell division (PubMed:21596567). In oocytes, plasma membrane localization of rme-2 is impaired resulting in the loss of vit-2 uptake which accumulates in the pseudocoelom (PubMed:21596567).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074052 – 181ADP-ribosylation factor 1-like 2Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiQ10943
PaxDbiQ10943
PeptideAtlasiQ10943
PRIDEiQ10943

Expressioni

Tissue specificityi

Expressed in hypodermis, intestine, spermatheca, uterus, gonadal sheath, vulva cells, pharynx muscle, body wall muscle, head neurons, ventral nerve cord.1 Publication

Developmental stagei

First detected in very young embryos with as little as 48-64 cells.1 Publication

Gene expression databases

BgeeiWBGene00000182

Interactioni

Protein-protein interaction databases

BioGridi41027, 6 interactors
DIPiDIP-24528N
IntActiQ10943, 3 interactors
STRINGi6239.B0336.2.1

Structurei

3D structure databases

ProteinModelPortaliQ10943
SMRiQ10943
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
InParanoidiQ10943
KOiK07937
OMAiAMREMAN
OrthoDBiEOG091G0S7S
PhylomeDBiQ10943

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNVFGSLFK GLFGKREMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
GEAREELMRM LAEDELRDAV LLVFANKQDL PQAMNAAEVT DKLGLHSLRN
160 170 180
RSWYIQATCA TSGDGLYEGL DWLSNQLKNR S
Length:181
Mass (Da):20,522
Last modified:January 23, 2007 - v2
Checksum:i98FD0886AD9A2459
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080138 Genomic DNA Translation: CCD61516.1
PIRiT15341
RefSeqiNP_498235.1, NM_065834.3
UniGeneiCel.6543

Genome annotation databases

EnsemblMetazoaiB0336.2.1; B0336.2.1; WBGene00000182
B0336.2.2; B0336.2.2; WBGene00000182
B0336.2.3; B0336.2.3; WBGene00000182
GeneIDi175801
KEGGicel:CELE_B0336.2
UCSCiB0336.2.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiARF12_CAEEL
AccessioniPrimary (citable) accession number: Q10943
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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