Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ADP-ribosylation factor 1-like 2

Gene

arf-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Involved in endoplasmic reticulum dynamics during embryogenesis (PubMed:15716356). Also required for adult germline function (PubMed:15576487). Plays a role in cell shedding during embryogenesis probably by promoting the endocytosis of cell adhesion molecules (PubMed:22801495).1 Publication2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: UniProtKB
  • gamete generation Source: UniProtKB
  • intracellular protein transport Source: WormBase
  • mitochondrion organization Source: WormBase
  • oocyte development Source: WormBase
  • regulation of apoptotic process involved in development Source: UniProtKB
  • regulation of receptor-mediated endocytosis Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1660499. Synthesis of PIPs at the plasma membrane.
R-CEL-1660514. Synthesis of PIPs at the Golgi membrane.
R-CEL-421837. Clathrin derived vesicle budding.
R-CEL-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1-like 2
Alternative name(s):
ADP-ribosylation factor-related protein 1.2
Gene namesi
Name:arf-1.2
Synonyms:arf-1
ORF Names:B0336.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiB0336.2; CE00696; WBGene00000182; arf-1.2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Death during embryogenesis. Some muscle differentiation occurs although it is poorly organized and little elongation or morphogenesis is evident.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074052 – 181ADP-ribosylation factor 1-like 2Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiQ10943.
PaxDbiQ10943.
PeptideAtlasiQ10943.
PRIDEiQ10943.

Expressioni

Tissue specificityi

Expressed in hypodermis, intestine, spermatheca, uterus, gonadal sheath, vulva cells, pharynx muscle, body wall muscle, head neurons, ventral nerve cord.1 Publication

Developmental stagei

First detected in very young embryos with as little as 48-64 cells.1 Publication

Gene expression databases

BgeeiWBGene00000182.

Interactioni

Protein-protein interaction databases

BioGridi41027. 6 interactors.
DIPiDIP-24528N.
IntActiQ10943. 3 interactors.
MINTiMINT-212315.
STRINGi6239.B0336.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ10943.
SMRiQ10943.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
InParanoidiQ10943.
KOiK07937.
OMAiNQEMRIL.
OrthoDBiEOG091G0S7S.
PhylomeDBiQ10943.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNVFGSLFK GLFGKREMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
GEAREELMRM LAEDELRDAV LLVFANKQDL PQAMNAAEVT DKLGLHSLRN
160 170 180
RSWYIQATCA TSGDGLYEGL DWLSNQLKNR S
Length:181
Mass (Da):20,522
Last modified:January 23, 2007 - v2
Checksum:i98FD0886AD9A2459
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080138 Genomic DNA. Translation: CCD61516.1.
PIRiT15341.
RefSeqiNP_498235.1. NM_065834.3.
UniGeneiCel.6543.

Genome annotation databases

EnsemblMetazoaiB0336.2.1; B0336.2.1; WBGene00000182.
B0336.2.2; B0336.2.2; WBGene00000182.
GeneIDi175801.
KEGGicel:CELE_B0336.2.
UCSCiB0336.2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080138 Genomic DNA. Translation: CCD61516.1.
PIRiT15341.
RefSeqiNP_498235.1. NM_065834.3.
UniGeneiCel.6543.

3D structure databases

ProteinModelPortaliQ10943.
SMRiQ10943.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41027. 6 interactors.
DIPiDIP-24528N.
IntActiQ10943. 3 interactors.
MINTiMINT-212315.
STRINGi6239.B0336.2.1.

Proteomic databases

EPDiQ10943.
PaxDbiQ10943.
PeptideAtlasiQ10943.
PRIDEiQ10943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0336.2.1; B0336.2.1; WBGene00000182.
B0336.2.2; B0336.2.2; WBGene00000182.
GeneIDi175801.
KEGGicel:CELE_B0336.2.
UCSCiB0336.2.1. c. elegans.

Organism-specific databases

CTDi175801.
WormBaseiB0336.2; CE00696; WBGene00000182; arf-1.2.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
InParanoidiQ10943.
KOiK07937.
OMAiNQEMRIL.
OrthoDBiEOG091G0S7S.
PhylomeDBiQ10943.

Enzyme and pathway databases

ReactomeiR-CEL-1660499. Synthesis of PIPs at the plasma membrane.
R-CEL-1660514. Synthesis of PIPs at the Golgi membrane.
R-CEL-421837. Clathrin derived vesicle budding.
R-CEL-6811438. Intra-Golgi traffic.

Miscellaneous databases

PROiQ10943.

Gene expression databases

BgeeiWBGene00000182.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF12_CAEEL
AccessioniPrimary (citable) accession number: Q10943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.