Q10925 (KIN25_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tyrosine-protein kinase kin-25
EC=2.7.10.2

Gene names

Name:	kin-25
ORF Names:	B0302.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1237 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA interference Inferred from mutant phenotype PubMed 22912399. Source: WormBase
Cellular_component	cytoplasm Inferred from direct assay PubMed 22912399. Source: WormBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q10925-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: Q10925-2)

The sequence of this isoform differs from the canonical sequence as follows:
     482-588: Missing.

Note: No experimental confirmation available.

Isoform c (identifier: Q10925-3)

The sequence of this isoform differs from the canonical sequence as follows:
     107-211: IKLYELIGEG...LLVSRLHDYC → TLHRSNSDRS...QLLRHHQLRC
     212-1045: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1237	1237	Probable tyrosine-protein kinase kin-25		PRO_0000088119
Regions
	Domain	107 – 369	263	Protein kinase
	Domain	366 – 426	61	SH3
	Nucleotide binding	113 – 121	9	ATP By similarity
Sites
	Active site	230	1	Proton acceptor By similarity
	Binding site	139	1	ATP By similarity
Natural variations
	Alternative sequence	107 – 211	105	IKLYE…LHDYC → TLHRSNSDRSHRHLFQRSNH NPFNTFRSLSNRNKFVYRHQ QLPFRNQFKSQLLPIVMWHP QLHLKRLQMHAIRYLQKQAH QLATRLSQLLLFTRHQLLRH HQLRC in isoform c.		VSP_014534
	Alternative sequence	212 – 1045	834	Missing in isoform c.		VSP_014535
	Alternative sequence	482 – 588	107	Missing in isoform b.		VSP_004999

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 05531196D8F865CB	FASTA	1,237	134,491
	10 20 30 40 50 60 MASTSGALVD DNVLEVLRKA QLDAFISQFV FLFNVRRFDH FSHVRDKDML EIGMQQVQIR 70 80 90 100 110 120 QLREQILKMS REMWNRSDPK QVYIQADQSM PAQNSIDEKA LIPNEQIKLY ELIGEGSFAV 130 140 150 160 170 180 VKRGTWTQSN GTHVNVAVKI LRDISPNIMD DLRVEASHLL KLQHPSLIRL YGIVRQPAMM 190 200 210 220 230 240 VFELCEGGSL LDRLRDDKKA ILLVSRLHDY CMQIAKALQF LESKHCVHRD VAARNILLAR 250 260 270 280 290 300 DERTVKICDF GLMRALKENE QMYTMAPQKK VPFAWCPPEA LRHRKFSHAS DVWSYGVTIW 310 320 330 340 350 360 EVFTFGEEPW VGCRAIDVLK NIDAGERLEK PKYCSERIYQ IMKNCWKFNP AERCKFGAIR 370 380 390 400 410 420 EDLVAAMFLD AVARETYNSI QPGALQLTKG DEVVVVENTG QDWFGQNKKN QKFGTFPRSV 430 440 450 460 470 480 VFAQTNNAVA AATAVTPQKV PTAPTIRIPP SHPPPAPLKP LNNNTKTSLN DRTSKISMPV 490 500 510 520 530 540 AGSFIHTGHG DPLGGQSWGN PATIADMYLK NPVNGAPLSS MSSGAEIIAS KELLTNGGRS 550 560 570 580 590 600 THQPAAPSPA VMSKIRGLSL DLPEYDDFDR AFDDGFSPSK IELPREFCGN DSVISGGSNS 610 620 630 640 650 660 IGLANTYVME PPKQAFDIRG NRVLPPTNKA PVLIPTNPAP SVISSTASAG ITLSTNSSQM 670 680 690 700 710 720 FTSQDRHSNM PANLFPELQH RLNQGSSTGN GVRPRPASSI GIQNNDLSML NPQVNRPFSV 730 740 750 760 770 780 VNVPIVQQPA NIPCLVPTPA PPAPAHFSQP VSSQRVAQQQ QNTLQKALND ELKGNLNKRP 790 800 810 820 830 840 TGTTAPPSNG FNAPRADVAP VQQRPISSAS IPALQPQPIQ HIQKPIQPQQ VRIPPSTAPV 850 860 870 880 890 900 QKPVQVSAPT HSNVAPTTSS QASADARNPL PPKTSPPVSN TPITVAPVHA APTTSAPSTS 910 920 930 940 950 960 VVTRRPTSTT AQMSDEERRS RIAMDISSAL PAPSALLYGS NSTSSLPSAA VSTASSVPST 970 980 990 1000 1010 1020 ARDNPVETRP SQPHVTMPPK KSSEPILSSE VLQPTRLPSA TTSQAKPVTQ PIRHPSPPVA 1030 1040 1050 1060 1070 1080 TVIPTAVVDK KPVSQNQGSN VPLFNITNSS NGYPQLNGYP NYGNGFQAYG YGMNYHQGYP 1090 1100 1110 1120 1130 1140 GYQGYNSYGN GMGQLALTHN AVTSLPPLVP SENRFSGTAQ PLGESDIMEF LGTQQRQAGS 1150 1160 1170 1180 1190 1200 SSRAVPPASA STSAASGITD LSMADKMEVL YREADFTHKG NCDTMVSQCN GNTEQALKLL 1210 1220 1230 KQQHLVDMEL AMSTETARQA LEARQYDLPA AANMLLG
	Isoform b [UniParc]. Checksum: 0074D7ACF67C3059	FASTA	1,130	123,190
	10 20 30 40 50 60 MASTSGALVD DNVLEVLRKA QLDAFISQFV FLFNVRRFDH FSHVRDKDML EIGMQQVQIR 70 80 90 100 110 120 QLREQILKMS REMWNRSDPK QVYIQADQSM PAQNSIDEKA LIPNEQIKLY ELIGEGSFAV 130 140 150 160 170 180 VKRGTWTQSN GTHVNVAVKI LRDISPNIMD DLRVEASHLL KLQHPSLIRL YGIVRQPAMM 190 200 210 220 230 240 VFELCEGGSL LDRLRDDKKA ILLVSRLHDY CMQIAKALQF LESKHCVHRD VAARNILLAR 250 260 270 280 290 300 DERTVKICDF GLMRALKENE QMYTMAPQKK VPFAWCPPEA LRHRKFSHAS DVWSYGVTIW 310 320 330 340 350 360 EVFTFGEEPW VGCRAIDVLK NIDAGERLEK PKYCSERIYQ IMKNCWKFNP AERCKFGAIR 370 380 390 400 410 420 EDLVAAMFLD AVARETYNSI QPGALQLTKG DEVVVVENTG QDWFGQNKKN QKFGTFPRSV 430 440 450 460 470 480 VFAQTNNAVA AATAVTPQKV PTAPTIRIPP SHPPPAPLKP LNNNTKTSLN DRTSKISMPV 490 500 510 520 530 540 AGNDSVISGG SNSIGLANTY VMEPPKQAFD IRGNRVLPPT NKAPVLIPTN PAPSVISSTA 550 560 570 580 590 600 SAGITLSTNS SQMFTSQDRH SNMPANLFPE LQHRLNQGSS TGNGVRPRPA SSIGIQNNDL 610 620 630 640 650 660 SMLNPQVNRP FSVVNVPIVQ QPANIPCLVP TPAPPAPAHF SQPVSSQRVA QQQQNTLQKA 670 680 690 700 710 720 LNDELKGNLN KRPTGTTAPP SNGFNAPRAD VAPVQQRPIS SASIPALQPQ PIQHIQKPIQ 730 740 750 760 770 780 PQQVRIPPST APVQKPVQVS APTHSNVAPT TSSQASADAR NPLPPKTSPP VSNTPITVAP 790 800 810 820 830 840 VHAAPTTSAP STSVVTRRPT STTAQMSDEE RRSRIAMDIS SALPAPSALL YGSNSTSSLP 850 860 870 880 890 900 SAAVSTASSV PSTARDNPVE TRPSQPHVTM PPKKSSEPIL SSEVLQPTRL PSATTSQAKP 910 920 930 940 950 960 VTQPIRHPSP PVATVIPTAV VDKKPVSQNQ GSNVPLFNIT NSSNGYPQLN GYPNYGNGFQ 970 980 990 1000 1010 1020 AYGYGMNYHQ GYPGYQGYNS YGNGMGQLAL THNAVTSLPP LVPSENRFSG TAQPLGESDI 1030 1040 1050 1060 1070 1080 MEFLGTQQRQ AGSSSRAVPP ASASTSAASG ITDLSMADKM EVLYREADFT HKGNCDTMVS 1090 1100 1110 1120 1130 QCNGNTEQAL KLLKQQHLVD MELAMSTETA RQALEARQYD LPAAANMLLG
	Isoform c [UniParc]. Checksum: 8DDFD3D44D77A0E2	FASTA	403	46,040
	10 20 30 40 50 60 MASTSGALVD DNVLEVLRKA QLDAFISQFV FLFNVRRFDH FSHVRDKDML EIGMQQVQIR 70 80 90 100 110 120 QLREQILKMS REMWNRSDPK QVYIQADQSM PAQNSIDEKA LIPNEQTLHR SNSDRSHRHL 130 140 150 160 170 180 FQRSNHNPFN TFRSLSNRNK FVYRHQQLPF RNQFKSQLLP IVMWHPQLHL KRLQMHAIRY 190 200 210 220 230 240 LQKQAHQLAT RLSQLLLFTR HQLLRHHQLR CITNSSNGYP QLNGYPNYGN GFQAYGYGMN 250 260 270 280 290 300 YHQGYPGYQG YNSYGNGMGQ LALTHNAVTS LPPLVPSENR FSGTAQPLGE SDIMEFLGTQ 310 320 330 340 350 360 QRQAGSSSRA VPPASASTSA ASGITDLSMA DKMEVLYREA DFTHKGNCDT MVSQCNGNTE 370 380 390 400 QALKLLKQQH LVDMELAMST ETARQALEAR QYDLPAAANM LLG

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080160 Genomic DNA. Translation: CCD61695.1. FO080160 Genomic DNA. Translation: CCD61696.1. FO080160 Genomic DNA. Translation: CCD61697.1.
PIR	T15316.
RefSeq	NP_510783.2. NM_078382.6. NP_510784.2. NM_078383.4. NP_872245.2. NM_182445.2.
UniGene	Cel.7063.
3D structure databases
ProteinModelPortal	Q10925.
SMR	Q10925. Positions 39-387.
ModBase	Search...
Proteomic databases
PaxDb	Q10925.
PRIDE	Q10925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0302.1a.1; B0302.1a.1; B0302.1. B0302.1a.2; B0302.1a.2; B0302.1.
GeneID	181756.
KEGG	cel:CELE_B0302.1.
UCSC	B0302.1b. c. elegans.
Organism-specific databases
CTD	181756.
WormBase	B0302.1a; CE33506; WBGene00002207; kin-25. B0302.1b; CE33548; WBGene00002207; kin-25. B0302.1c; CE35209; WBGene00002207; kin-25.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00630000089536.
HOGENOM	HOG000020761.
InParanoid	Q10925.
OMA	FLESKHC.
Gene expression databases
ArrayExpress	Q10925.
Family and domain databases
Gene3D	4.10.680.10. 1 hit.
InterPro	IPR015116. Cdc42_binding_dom_like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR011511. SH3_2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF09027. GTPase_binding. 1 hit. PF07714. Pkinase_Tyr. 1 hit. PF07653. SH3_2. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SMART	SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	915210.

Entry information

Entry name

KIN25_CAEEL

Accession

Primary (citable) accession number: Q10925
Secondary accession number(s): Q7JP57 , Q7JP58, Q7JP59, Q95ZZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project