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Q10925

- SID3_CAEEL

UniProt

Q10925 - SID3_CAEEL

Protein

Tyrosine-protein kinase sid-3

Gene

sid-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Tyrosine-protein kinase which plays a role in RNA-mediated gene silencing by mediating import of double-stranded RNA (dsRNA) into cells. Not required for import of ingested dsRNA into intestinal cells but involved in subsequent export from intestinal cells to internal tissues.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei139 – 1391ATPPROSITE-ProRule annotation
    Active sitei230 – 2301Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi113 – 1219ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. RNA interference Source: WormBase

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    RNA-mediated gene silencing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase sid-3 (EC:2.7.10.2)
    Alternative name(s):
    Systemic RNA interference defective protein 3
    Systemic RNAi enabling protein
    Gene namesi
    Name:sid-3
    ORF Names:B0302.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiB0302.1a; CE33506; WBGene00002207; sid-3.
    B0302.1b; CE33548; WBGene00002207; sid-3.
    B0302.1c; CE35209; WBGene00002207; sid-3.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Displays diffuse localization in cytoplasm and cytoplasmic foci, and also in a punctate pattern.

    GO - Cellular componenti

    1. cytoplasm Source: WormBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Defective in transport of silencing RNAs but not defective in execution of RNAi. Marginal enhancement of cell-autonomous RNAi. Mildly defective in silencing the intestine-expressed gene, act-5.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi124 – 1241G → D in qt32; induces defects in mobile RNA silencing. 1 Publication
    Mutagenesisi139 – 1391K → A: Failure to rescue silencing defect in sid-3 mutants. 1 Publication
    Mutagenesisi353 – 3531R → Q in qt38; induces defects in mobile RNA silencing. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12371237Tyrosine-protein kinase sid-3PRO_0000088119Add
    BLAST

    Proteomic databases

    PaxDbiQ10925.
    PRIDEiQ10925.

    Expressioni

    Tissue specificityi

    Ubiquitously present in all tissues tested. Expressed in the somatic cells of gut, pharynx, body wall muscle, neurons, skin and excretory canal cells.1 Publication

    Developmental stagei

    Expressed throughout all developmental stages.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ10925.
    SMRiQ10925. Positions 39-387.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini107 – 369263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini366 – 42661SH3Add
    BLAST

    Domaini

    The kinase domain is required for import of dsRNA into cells.1 Publication

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.Curated

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115195.
    HOGENOMiHOG000020761.
    InParanoidiQ10925.
    OMAiFLESKHC.

    Family and domain databases

    Gene3Di4.10.680.10. 1 hit.
    InterProiIPR015116. Cdc42_binding_dom_like.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF09027. GTPase_binding. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SMARTiSM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q10925-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASTSGALVD DNVLEVLRKA QLDAFISQFV FLFNVRRFDH FSHVRDKDML     50
    EIGMQQVQIR QLREQILKMS REMWNRSDPK QVYIQADQSM PAQNSIDEKA 100
    LIPNEQIKLY ELIGEGSFAV VKRGTWTQSN GTHVNVAVKI LRDISPNIMD 150
    DLRVEASHLL KLQHPSLIRL YGIVRQPAMM VFELCEGGSL LDRLRDDKKA 200
    ILLVSRLHDY CMQIAKALQF LESKHCVHRD VAARNILLAR DERTVKICDF 250
    GLMRALKENE QMYTMAPQKK VPFAWCPPEA LRHRKFSHAS DVWSYGVTIW 300
    EVFTFGEEPW VGCRAIDVLK NIDAGERLEK PKYCSERIYQ IMKNCWKFNP 350
    AERCKFGAIR EDLVAAMFLD AVARETYNSI QPGALQLTKG DEVVVVENTG 400
    QDWFGQNKKN QKFGTFPRSV VFAQTNNAVA AATAVTPQKV PTAPTIRIPP 450
    SHPPPAPLKP LNNNTKTSLN DRTSKISMPV AGSFIHTGHG DPLGGQSWGN 500
    PATIADMYLK NPVNGAPLSS MSSGAEIIAS KELLTNGGRS THQPAAPSPA 550
    VMSKIRGLSL DLPEYDDFDR AFDDGFSPSK IELPREFCGN DSVISGGSNS 600
    IGLANTYVME PPKQAFDIRG NRVLPPTNKA PVLIPTNPAP SVISSTASAG 650
    ITLSTNSSQM FTSQDRHSNM PANLFPELQH RLNQGSSTGN GVRPRPASSI 700
    GIQNNDLSML NPQVNRPFSV VNVPIVQQPA NIPCLVPTPA PPAPAHFSQP 750
    VSSQRVAQQQ QNTLQKALND ELKGNLNKRP TGTTAPPSNG FNAPRADVAP 800
    VQQRPISSAS IPALQPQPIQ HIQKPIQPQQ VRIPPSTAPV QKPVQVSAPT 850
    HSNVAPTTSS QASADARNPL PPKTSPPVSN TPITVAPVHA APTTSAPSTS 900
    VVTRRPTSTT AQMSDEERRS RIAMDISSAL PAPSALLYGS NSTSSLPSAA 950
    VSTASSVPST ARDNPVETRP SQPHVTMPPK KSSEPILSSE VLQPTRLPSA 1000
    TTSQAKPVTQ PIRHPSPPVA TVIPTAVVDK KPVSQNQGSN VPLFNITNSS 1050
    NGYPQLNGYP NYGNGFQAYG YGMNYHQGYP GYQGYNSYGN GMGQLALTHN 1100
    AVTSLPPLVP SENRFSGTAQ PLGESDIMEF LGTQQRQAGS SSRAVPPASA 1150
    STSAASGITD LSMADKMEVL YREADFTHKG NCDTMVSQCN GNTEQALKLL 1200
    KQQHLVDMEL AMSTETARQA LEARQYDLPA AANMLLG 1237

    Note: No experimental confirmation available.

    Length:1,237
    Mass (Da):134,491
    Last modified:July 5, 2005 - v2
    Checksum:i05531196D8F865CB
    GO
    Isoform b (identifier: Q10925-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         482-588: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,130
    Mass (Da):123,190
    Checksum:i0074D7ACF67C3059
    GO
    Isoform c (identifier: Q10925-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         107-211: IKLYELIGEG...LLVSRLHDYC → TLHRSNSDRS...QLLRHHQLRC
         212-1045: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:403
    Mass (Da):46,040
    Checksum:i8DDFD3D44D77A0E2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei107 – 211105IKLYE…LHDYC → TLHRSNSDRSHRHLFQRSNH NPFNTFRSLSNRNKFVYRHQ QLPFRNQFKSQLLPIVMWHP QLHLKRLQMHAIRYLQKQAH QLATRLSQLLLFTRHQLLRH HQLRC in isoform c. CuratedVSP_014534Add
    BLAST
    Alternative sequencei212 – 1045834Missing in isoform c. CuratedVSP_014535Add
    BLAST
    Alternative sequencei482 – 588107Missing in isoform b. CuratedVSP_004999Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080160 Genomic DNA. Translation: CCD61695.1.
    FO080160 Genomic DNA. Translation: CCD61696.1.
    FO080160 Genomic DNA. Translation: CCD61697.1.
    PIRiT15316.
    RefSeqiNP_510783.2. NM_078382.6. [Q10925-1]
    NP_510784.2. NM_078383.4. [Q10925-2]
    NP_872245.2. NM_182445.2. [Q10925-3]
    UniGeneiCel.7063.

    Genome annotation databases

    EnsemblMetazoaiB0302.1a.1; B0302.1a.1; WBGene00002207. [Q10925-1]
    B0302.1a.2; B0302.1a.2; WBGene00002207. [Q10925-1]
    GeneIDi181756.
    KEGGicel:CELE_B0302.1.
    UCSCiB0302.1b. c. elegans. [Q10925-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080160 Genomic DNA. Translation: CCD61695.1 .
    FO080160 Genomic DNA. Translation: CCD61696.1 .
    FO080160 Genomic DNA. Translation: CCD61697.1 .
    PIRi T15316.
    RefSeqi NP_510783.2. NM_078382.6. [Q10925-1 ]
    NP_510784.2. NM_078383.4. [Q10925-2 ]
    NP_872245.2. NM_182445.2. [Q10925-3 ]
    UniGenei Cel.7063.

    3D structure databases

    ProteinModelPortali Q10925.
    SMRi Q10925. Positions 39-387.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q10925.
    PRIDEi Q10925.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai B0302.1a.1 ; B0302.1a.1 ; WBGene00002207 . [Q10925-1 ]
    B0302.1a.2 ; B0302.1a.2 ; WBGene00002207 . [Q10925-1 ]
    GeneIDi 181756.
    KEGGi cel:CELE_B0302.1.
    UCSCi B0302.1b. c. elegans. [Q10925-1 ]

    Organism-specific databases

    CTDi 181756.
    WormBasei B0302.1a ; CE33506 ; WBGene00002207 ; sid-3.
    B0302.1b ; CE33548 ; WBGene00002207 ; sid-3.
    B0302.1c ; CE35209 ; WBGene00002207 ; sid-3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115195.
    HOGENOMi HOG000020761.
    InParanoidi Q10925.
    OMAi FLESKHC.

    Miscellaneous databases

    NextBioi 915210.
    PROi Q10925.

    Family and domain databases

    Gene3Di 4.10.680.10. 1 hit.
    InterProi IPR015116. Cdc42_binding_dom_like.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF09027. GTPase_binding. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SMARTi SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "Conserved tyrosine kinase promotes the import of silencing RNA into Caenorhabditis elegans cells."
      Jose A.M., Kim Y.A., Leal-Ekman S., Hunter C.P.
      Proc. Natl. Acad. Sci. U.S.A. 109:14520-14525(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DOMAIN, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-124; LYS-139 AND ARG-353.

    Entry informationi

    Entry nameiSID3_CAEEL
    AccessioniPrimary (citable) accession number: Q10925
    Secondary accession number(s): Q7JP57
    , Q7JP58, Q7JP59, Q95ZZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3