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Protein

Tyrosine-protein kinase sid-3

Gene

sid-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine-protein kinase which plays a role in RNA-mediated gene silencing by mediating import of double-stranded RNA (dsRNA) into cells. Not required for import of ingested dsRNA into intestinal cells but involved in subsequent export from intestinal cells to internal tissues.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei139 – 1391ATPPROSITE-ProRule annotation
Active sitei230 – 2301Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi113 – 1219ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  3. receptor binding Source: GO_Central

GO - Biological processi

  1. cell differentiation Source: GO_Central
  2. cell migration Source: GO_Central
  3. innate immune response Source: GO_Central
  4. peptidyl-tyrosine autophosphorylation Source: GO_Central
  5. regulation of cell proliferation Source: GO_Central
  6. RNA interference Source: WormBase
  7. transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase sid-3 (EC:2.7.10.2)
Alternative name(s):
Systemic RNA interference defective protein 3
Systemic RNAi enabling protein
Gene namesi
Name:sid-3
ORF Names:B0302.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiB0302.1a; CE33506; WBGene00002207; sid-3.
B0302.1b; CE33548; WBGene00002207; sid-3.
B0302.1c; CE35209; WBGene00002207; sid-3.

Subcellular locationi

  1. Cytoplasm 1 Publication

  2. Note: Displays diffuse localization in cytoplasm and cytoplasmic foci, and also in a punctate pattern.

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Defective in transport of silencing RNAs but not defective in execution of RNAi. Marginal enhancement of cell-autonomous RNAi. Mildly defective in silencing the intestine-expressed gene, act-5.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi124 – 1241G → D in qt32; induces defects in mobile RNA silencing. 1 Publication
Mutagenesisi139 – 1391K → A: Failure to rescue silencing defect in sid-3 mutants. 1 Publication
Mutagenesisi353 – 3531R → Q in qt38; induces defects in mobile RNA silencing. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12371237Tyrosine-protein kinase sid-3PRO_0000088119Add
BLAST

Proteomic databases

PaxDbiQ10925.
PRIDEiQ10925.

Expressioni

Tissue specificityi

Ubiquitously present in all tissues tested. Expressed in the somatic cells of gut, pharynx, body wall muscle, neurons, skin and excretory canal cells.1 Publication

Developmental stagei

Expressed throughout all developmental stages.1 Publication

Gene expression databases

ExpressionAtlasiQ10925. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ10925.
SMRiQ10925. Positions 39-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 369263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini366 – 42661SH3Add
BLAST

Domaini

The kinase domain is required for import of dsRNA into cells.1 Publication

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118799.
HOGENOMiHOG000020761.
InParanoidiQ10925.
KOiK08886.
OMAiFLESKHC.

Family and domain databases

Gene3Di4.10.680.10. 1 hit.
InterProiIPR015116. Cdc42_binding_dom_like.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF09027. GTPase_binding. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q10925-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTSGALVD DNVLEVLRKA QLDAFISQFV FLFNVRRFDH FSHVRDKDML
60 70 80 90 100
EIGMQQVQIR QLREQILKMS REMWNRSDPK QVYIQADQSM PAQNSIDEKA
110 120 130 140 150
LIPNEQIKLY ELIGEGSFAV VKRGTWTQSN GTHVNVAVKI LRDISPNIMD
160 170 180 190 200
DLRVEASHLL KLQHPSLIRL YGIVRQPAMM VFELCEGGSL LDRLRDDKKA
210 220 230 240 250
ILLVSRLHDY CMQIAKALQF LESKHCVHRD VAARNILLAR DERTVKICDF
260 270 280 290 300
GLMRALKENE QMYTMAPQKK VPFAWCPPEA LRHRKFSHAS DVWSYGVTIW
310 320 330 340 350
EVFTFGEEPW VGCRAIDVLK NIDAGERLEK PKYCSERIYQ IMKNCWKFNP
360 370 380 390 400
AERCKFGAIR EDLVAAMFLD AVARETYNSI QPGALQLTKG DEVVVVENTG
410 420 430 440 450
QDWFGQNKKN QKFGTFPRSV VFAQTNNAVA AATAVTPQKV PTAPTIRIPP
460 470 480 490 500
SHPPPAPLKP LNNNTKTSLN DRTSKISMPV AGSFIHTGHG DPLGGQSWGN
510 520 530 540 550
PATIADMYLK NPVNGAPLSS MSSGAEIIAS KELLTNGGRS THQPAAPSPA
560 570 580 590 600
VMSKIRGLSL DLPEYDDFDR AFDDGFSPSK IELPREFCGN DSVISGGSNS
610 620 630 640 650
IGLANTYVME PPKQAFDIRG NRVLPPTNKA PVLIPTNPAP SVISSTASAG
660 670 680 690 700
ITLSTNSSQM FTSQDRHSNM PANLFPELQH RLNQGSSTGN GVRPRPASSI
710 720 730 740 750
GIQNNDLSML NPQVNRPFSV VNVPIVQQPA NIPCLVPTPA PPAPAHFSQP
760 770 780 790 800
VSSQRVAQQQ QNTLQKALND ELKGNLNKRP TGTTAPPSNG FNAPRADVAP
810 820 830 840 850
VQQRPISSAS IPALQPQPIQ HIQKPIQPQQ VRIPPSTAPV QKPVQVSAPT
860 870 880 890 900
HSNVAPTTSS QASADARNPL PPKTSPPVSN TPITVAPVHA APTTSAPSTS
910 920 930 940 950
VVTRRPTSTT AQMSDEERRS RIAMDISSAL PAPSALLYGS NSTSSLPSAA
960 970 980 990 1000
VSTASSVPST ARDNPVETRP SQPHVTMPPK KSSEPILSSE VLQPTRLPSA
1010 1020 1030 1040 1050
TTSQAKPVTQ PIRHPSPPVA TVIPTAVVDK KPVSQNQGSN VPLFNITNSS
1060 1070 1080 1090 1100
NGYPQLNGYP NYGNGFQAYG YGMNYHQGYP GYQGYNSYGN GMGQLALTHN
1110 1120 1130 1140 1150
AVTSLPPLVP SENRFSGTAQ PLGESDIMEF LGTQQRQAGS SSRAVPPASA
1160 1170 1180 1190 1200
STSAASGITD LSMADKMEVL YREADFTHKG NCDTMVSQCN GNTEQALKLL
1210 1220 1230
KQQHLVDMEL AMSTETARQA LEARQYDLPA AANMLLG

Note: No experimental confirmation available.

Length:1,237
Mass (Da):134,491
Last modified:July 5, 2005 - v2
Checksum:i05531196D8F865CB
GO
Isoform b (identifier: Q10925-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     482-588: Missing.

Note: No experimental confirmation available.

Show »
Length:1,130
Mass (Da):123,190
Checksum:i0074D7ACF67C3059
GO
Isoform c (identifier: Q10925-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     107-211: IKLYELIGEG...LLVSRLHDYC → TLHRSNSDRS...QLLRHHQLRC
     212-1045: Missing.

Note: No experimental confirmation available.

Show »
Length:403
Mass (Da):46,040
Checksum:i8DDFD3D44D77A0E2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei107 – 211105IKLYE…LHDYC → TLHRSNSDRSHRHLFQRSNH NPFNTFRSLSNRNKFVYRHQ QLPFRNQFKSQLLPIVMWHP QLHLKRLQMHAIRYLQKQAH QLATRLSQLLLFTRHQLLRH HQLRC in isoform c. CuratedVSP_014534Add
BLAST
Alternative sequencei212 – 1045834Missing in isoform c. CuratedVSP_014535Add
BLAST
Alternative sequencei482 – 588107Missing in isoform b. CuratedVSP_004999Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080160 Genomic DNA. Translation: CCD61695.1.
FO080160 Genomic DNA. Translation: CCD61696.1.
FO080160 Genomic DNA. Translation: CCD61697.1.
PIRiT15316.
RefSeqiNP_510783.2. NM_078382.6. [Q10925-1]
NP_510784.2. NM_078383.4. [Q10925-2]
NP_872245.2. NM_182445.2. [Q10925-3]
UniGeneiCel.7063.

Genome annotation databases

EnsemblMetazoaiB0302.1a.1; B0302.1a.1; WBGene00002207. [Q10925-1]
B0302.1a.2; B0302.1a.2; WBGene00002207. [Q10925-1]
GeneIDi181756.
KEGGicel:CELE_B0302.1.
UCSCiB0302.1b. c. elegans. [Q10925-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080160 Genomic DNA. Translation: CCD61695.1.
FO080160 Genomic DNA. Translation: CCD61696.1.
FO080160 Genomic DNA. Translation: CCD61697.1.
PIRiT15316.
RefSeqiNP_510783.2. NM_078382.6. [Q10925-1]
NP_510784.2. NM_078383.4. [Q10925-2]
NP_872245.2. NM_182445.2. [Q10925-3]
UniGeneiCel.7063.

3D structure databases

ProteinModelPortaliQ10925.
SMRiQ10925. Positions 39-387.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ10925.
PRIDEiQ10925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0302.1a.1; B0302.1a.1; WBGene00002207. [Q10925-1]
B0302.1a.2; B0302.1a.2; WBGene00002207. [Q10925-1]
GeneIDi181756.
KEGGicel:CELE_B0302.1.
UCSCiB0302.1b. c. elegans. [Q10925-1]

Organism-specific databases

CTDi181756.
WormBaseiB0302.1a; CE33506; WBGene00002207; sid-3.
B0302.1b; CE33548; WBGene00002207; sid-3.
B0302.1c; CE35209; WBGene00002207; sid-3.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118799.
HOGENOMiHOG000020761.
InParanoidiQ10925.
KOiK08886.
OMAiFLESKHC.

Miscellaneous databases

NextBioi915210.
PROiQ10925.

Gene expression databases

ExpressionAtlasiQ10925. baseline.

Family and domain databases

Gene3Di4.10.680.10. 1 hit.
InterProiIPR015116. Cdc42_binding_dom_like.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF09027. GTPase_binding. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "Conserved tyrosine kinase promotes the import of silencing RNA into Caenorhabditis elegans cells."
    Jose A.M., Kim Y.A., Leal-Ekman S., Hunter C.P.
    Proc. Natl. Acad. Sci. U.S.A. 109:14520-14525(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DOMAIN, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-124; LYS-139 AND ARG-353.

Entry informationi

Entry nameiSID3_CAEEL
AccessioniPrimary (citable) accession number: Q10925
Secondary accession number(s): Q7JP57
, Q7JP58, Q7JP59, Q95ZZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 5, 2005
Last modified: April 29, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.