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Q10925 (SID3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase sid-3

EC=2.7.10.2
Alternative name(s):
Systemic RNA interference defective protein 3
Systemic RNAi enabling protein
Gene names
Name:sid-3
ORF Names:B0302.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1237 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine-protein kinase which plays a role in RNA-mediated gene silencing by mediating import of double-stranded RNA (dsRNA) into cells. Not required for import of ingested dsRNA into intestinal cells but involved in subsequent export from intestinal cells to internal tissues. Ref.2

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Cytoplasm. Note: Displays diffuse localization in cytoplasm and cytoplasmic foci, and also in a punctate pattern. Ref.2

Tissue specificity

Ubiquitously present in all tissues tested. Expressed in the somatic cells of gut, pharynx, body wall muscle, neurons, skin and excretory canal cells. Ref.2

Developmental stage

Expressed throughout all developmental stages. Ref.2

Domain

The kinase domain is required for import of dsRNA into cells. Ref.2

Disruption phenotype

Defective in transport of silencing RNAs but not defective in execution of RNAi. Marginal enhancement of cell-autonomous RNAi. Mildly defective in silencing the intestine-expressed gene, act-5. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.

Contains 1 protein kinase domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processRNA-mediated gene silencing
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainSH3 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Tyrosine-protein kinase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA interference

Inferred from mutant phenotype Ref.2. Source: WormBase

   Cellular_componentcytoplasm

Inferred from direct assay Ref.2. Source: WormBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q10925-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: Q10925-2)

The sequence of this isoform differs from the canonical sequence as follows:
     482-588: Missing.
Note: No experimental confirmation available.
Isoform c (identifier: Q10925-3)

The sequence of this isoform differs from the canonical sequence as follows:
     107-211: IKLYELIGEG...LLVSRLHDYC → TLHRSNSDRS...QLLRHHQLRC
     212-1045: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12371237Tyrosine-protein kinase sid-3
PRO_0000088119

Regions

Domain107 – 369263Protein kinase
Domain366 – 42661SH3
Nucleotide binding113 – 1219ATP By similarity

Sites

Active site2301Proton acceptor By similarity
Binding site1391ATP By similarity

Natural variations

Alternative sequence107 – 211105IKLYE…LHDYC → TLHRSNSDRSHRHLFQRSNH NPFNTFRSLSNRNKFVYRHQ QLPFRNQFKSQLLPIVMWHP QLHLKRLQMHAIRYLQKQAH QLATRLSQLLLFTRHQLLRH HQLRC in isoform c.
VSP_014534
Alternative sequence212 – 1045834Missing in isoform c.
VSP_014535
Alternative sequence482 – 588107Missing in isoform b.
VSP_004999

Experimental info

Mutagenesis1241G → D in qt32; induces defects in mobile RNA silencing. Ref.2
Mutagenesis1391K → A: Failure to rescue silencing defect in sid-3 mutants. Ref.2
Mutagenesis3531R → Q in qt38; induces defects in mobile RNA silencing. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: 05531196D8F865CB

FASTA1,237134,491
        10         20         30         40         50         60 
MASTSGALVD DNVLEVLRKA QLDAFISQFV FLFNVRRFDH FSHVRDKDML EIGMQQVQIR 

        70         80         90        100        110        120 
QLREQILKMS REMWNRSDPK QVYIQADQSM PAQNSIDEKA LIPNEQIKLY ELIGEGSFAV 

       130        140        150        160        170        180 
VKRGTWTQSN GTHVNVAVKI LRDISPNIMD DLRVEASHLL KLQHPSLIRL YGIVRQPAMM 

       190        200        210        220        230        240 
VFELCEGGSL LDRLRDDKKA ILLVSRLHDY CMQIAKALQF LESKHCVHRD VAARNILLAR 

       250        260        270        280        290        300 
DERTVKICDF GLMRALKENE QMYTMAPQKK VPFAWCPPEA LRHRKFSHAS DVWSYGVTIW 

       310        320        330        340        350        360 
EVFTFGEEPW VGCRAIDVLK NIDAGERLEK PKYCSERIYQ IMKNCWKFNP AERCKFGAIR 

       370        380        390        400        410        420 
EDLVAAMFLD AVARETYNSI QPGALQLTKG DEVVVVENTG QDWFGQNKKN QKFGTFPRSV 

       430        440        450        460        470        480 
VFAQTNNAVA AATAVTPQKV PTAPTIRIPP SHPPPAPLKP LNNNTKTSLN DRTSKISMPV 

       490        500        510        520        530        540 
AGSFIHTGHG DPLGGQSWGN PATIADMYLK NPVNGAPLSS MSSGAEIIAS KELLTNGGRS 

       550        560        570        580        590        600 
THQPAAPSPA VMSKIRGLSL DLPEYDDFDR AFDDGFSPSK IELPREFCGN DSVISGGSNS 

       610        620        630        640        650        660 
IGLANTYVME PPKQAFDIRG NRVLPPTNKA PVLIPTNPAP SVISSTASAG ITLSTNSSQM 

       670        680        690        700        710        720 
FTSQDRHSNM PANLFPELQH RLNQGSSTGN GVRPRPASSI GIQNNDLSML NPQVNRPFSV 

       730        740        750        760        770        780 
VNVPIVQQPA NIPCLVPTPA PPAPAHFSQP VSSQRVAQQQ QNTLQKALND ELKGNLNKRP 

       790        800        810        820        830        840 
TGTTAPPSNG FNAPRADVAP VQQRPISSAS IPALQPQPIQ HIQKPIQPQQ VRIPPSTAPV 

       850        860        870        880        890        900 
QKPVQVSAPT HSNVAPTTSS QASADARNPL PPKTSPPVSN TPITVAPVHA APTTSAPSTS 

       910        920        930        940        950        960 
VVTRRPTSTT AQMSDEERRS RIAMDISSAL PAPSALLYGS NSTSSLPSAA VSTASSVPST 

       970        980        990       1000       1010       1020 
ARDNPVETRP SQPHVTMPPK KSSEPILSSE VLQPTRLPSA TTSQAKPVTQ PIRHPSPPVA 

      1030       1040       1050       1060       1070       1080 
TVIPTAVVDK KPVSQNQGSN VPLFNITNSS NGYPQLNGYP NYGNGFQAYG YGMNYHQGYP 

      1090       1100       1110       1120       1130       1140 
GYQGYNSYGN GMGQLALTHN AVTSLPPLVP SENRFSGTAQ PLGESDIMEF LGTQQRQAGS 

      1150       1160       1170       1180       1190       1200 
SSRAVPPASA STSAASGITD LSMADKMEVL YREADFTHKG NCDTMVSQCN GNTEQALKLL 

      1210       1220       1230 
KQQHLVDMEL AMSTETARQA LEARQYDLPA AANMLLG 

« Hide

Isoform b [UniParc].

Checksum: 0074D7ACF67C3059
Show »

FASTA1,130123,190
Isoform c [UniParc].

Checksum: 8DDFD3D44D77A0E2
Show »

FASTA40346,040

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[2]"Conserved tyrosine kinase promotes the import of silencing RNA into Caenorhabditis elegans cells."
Jose A.M., Kim Y.A., Leal-Ekman S., Hunter C.P.
Proc. Natl. Acad. Sci. U.S.A. 109:14520-14525(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DOMAIN, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-124; LYS-139 AND ARG-353.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080160 Genomic DNA. Translation: CCD61695.1.
FO080160 Genomic DNA. Translation: CCD61696.1.
FO080160 Genomic DNA. Translation: CCD61697.1.
PIRT15316.
RefSeqNP_510783.2. NM_078382.6.
NP_510784.2. NM_078383.4.
NP_872245.2. NM_182445.2.
UniGeneCel.7063.

3D structure databases

ProteinModelPortalQ10925.
SMRQ10925. Positions 39-366.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ10925.
PRIDEQ10925.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaB0302.1a.1; B0302.1a.1; B0302.1. [Q10925-1]
B0302.1a.2; B0302.1a.2; B0302.1. [Q10925-1]
GeneID181756.
KEGGcel:CELE_B0302.1.
UCSCB0302.1b. c. elegans. [Q10925-1]

Organism-specific databases

CTD181756.
WormBaseB0302.1a; CE33506; WBGene00002207; sid-3.
B0302.1b; CE33548; WBGene00002207; sid-3.
B0302.1c; CE35209; WBGene00002207; sid-3.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000020761.
InParanoidQ10925.
OMAFLESKHC.

Family and domain databases

Gene3D4.10.680.10. 1 hit.
InterProIPR015116. Cdc42_binding_dom_like.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF09027. GTPase_binding. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SMARTSM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio915210.
PROQ10925.

Entry information

Entry nameSID3_CAEEL
AccessionPrimary (citable) accession number: Q10925
Secondary accession number(s): Q7JP57 expand/collapse secondary AC list , Q7JP58, Q7JP59, Q95ZZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase