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Protein

Excitatory amino acid transporter

Gene

glt-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity).By similarity

GO - Molecular functioni

  • L-glutamate transmembrane transporter activity Source: WormBase
  • symporter activity Source: UniProtKB-KW

Keywordsi

Biological processSymport, Transport

Enzyme and pathway databases

ReactomeiR-CEL-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
R-CEL-210500 Glutamate Neurotransmitter Release Cycle
R-CEL-425393 Transport of inorganic cations/anions and amino acids/oligopeptides

Protein family/group databases

TCDBi2.A.23.2.10 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter
Alternative name(s):
Sodium-dependent glutamate/ aspartate transporter
Gene namesi
Name:glt-1
ORF Names:C12D12.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC12D12.2a ; CE29083 ; WBGene00001620 ; glt-1
C12D12.2b ; CE29084 ; WBGene00001620 ; glt-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 198ExtracellularSequence analysisAdd BLAST82
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
Transmembranei400 – 420HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020741 – 503Excitatory amino acid transporterAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi177N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi187N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ10901
PaxDbiQ10901
PeptideAtlasiQ10901
PRIDEiQ10901

PTM databases

iPTMnetiQ10901

Expressioni

Gene expression databases

BgeeiWBGene00001620

Interactioni

Protein-protein interaction databases

STRINGi6239.C12D12.2a.1

Structurei

3D structure databases

ProteinModelPortaliQ10901
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787 Eukaryota
COG1301 LUCA
GeneTreeiENSGT00760000119117
HOGENOMiHOG000208776
InParanoidiQ10901
KOiK05613
OMAiYCSFASM
OrthoDBiEOG091G0UCE
PhylomeDBiQ10901

Family and domain databases

Gene3Di1.10.3860.10, 2 hits
InterProiView protein in InterPro
IPR001991 Na-dicarboxylate_symporter
IPR018107 Na-dicarboxylate_symporter_CS
IPR036458 Na:dicarbo_symporter_sf
PfamiView protein in Pfam
PF00375 SDF, 1 hit
SUPFAMiSSF118215 SSF118215, 2 hits
PROSITEiView protein in PROSITE
PS00713 NA_DICARBOXYL_SYMP_1, 1 hit
PS00714 NA_DICARBOXYL_SYMP_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q10901-1) [UniParc]FASTAAdd to basket
Also known as: Glt-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPDTRINKE IMVSWIRKNL LLVLTVSSVV LGALCGFLLR GLQLSPQNIM
60 70 80 90 100
YISFPGELLM HMLKMMILPL IMSSLISGLA QLDARQSGKL GSLAVTYYMF
110 120 130 140 150
TTAVAVVTGI FLVLVIHPGD PTIKKEIGTG TEGKTVSTVD TLLDLLRNMF
160 170 180 190 200
PENVVQATFQ QVQTKYIKVR PKVVKNNDSA TLAALNNGSL DYVKASVEYT
210 220 230 240 250
SGMNVLGVIV FCIAIGISLS QLGQEAHVMV QFFVIMDKVI MKLVMTVMWY
260 270 280 290 300
SPFGIFCLIM GKILEIHDLA DTARMLAMYM VTVLSGLAIH SLISLPLIFF
310 320 330 340 350
VTTKKNPYVF MRGLFQAWIT ALGTASSSAT LPITFNCLEE NLGVDRRVTR
360 370 380 390 400
FVLPVGATIN MDGTALYEAV AAIFIAQING VHLSFGQVVT VSLTATLASI
410 420 430 440 450
GAASVPSAGL VTMLLVLTAV GLPVKDVSLI VAVDWLLDRI RTSINVLGDA
460 470 480 490 500
MGAGIVYHYS KADLDAHDRL AATTRSHSIA MNDEKRQLAV YNSLPTDDEK

HTH
Length:503
Mass (Da):54,676
Last modified:July 15, 1998 - v2
Checksum:i3014BFF28E41E798
GO
Isoform b (identifier: Q10901-2) [UniParc]FASTAAdd to basket
Also known as: Glt-1

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Show »
Length:492
Mass (Da):53,380
Checksum:i5A4A53894038B1EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68L → P in AAB41909 (PubMed:8885221).Curated1
Sequence conflicti68L → P in AAB41910 (PubMed:8885221).Curated1
Sequence conflicti270 – 273ADTA → ENTT in AAB41909 (PubMed:8885221).Curated4
Sequence conflicti270 – 273ADTA → ENTT in AAB41910 (PubMed:8885221).Curated4
Sequence conflicti321A → G in AAB41909 (PubMed:8885221).Curated1
Sequence conflicti321A → G in AAB41910 (PubMed:8885221).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0062671 – 11Missing in isoform b. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35250 mRNA Translation: AAB41909.1
U35250 mRNA Translation: AAB41910.1
D86740 mRNA Translation: BAA13164.1
D86741 mRNA Translation: BAA21840.1
FO080514 Genomic DNA Translation: CCD64307.1
FO080514 Genomic DNA Translation: CCD64308.1
PIRiJC5078
RefSeqiNP_001024393.1, NM_001029222.3 [Q10901-1]
NP_001024394.1, NM_001029223.2 [Q10901-2]
UniGeneiCel.21360

Genome annotation databases

EnsemblMetazoaiC12D12.2a; C12D12.2a; WBGene00001620 [Q10901-1]
GeneIDi180641
KEGGicel:CELE_C12D12.2
UCSCiC12D12.2b.3 c. elegans [Q10901-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEAA1_CAEEL
AccessioniPrimary (citable) accession number: Q10901
Secondary accession number(s): P90798, Q17920
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health