Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Excitatory amino acid transporter

Gene

glt-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity).By similarity

GO - Molecular functioni

  • L-glutamate transmembrane transporter activity Source: WormBase
  • symporter activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-CEL-210500. Glutamate Neurotransmitter Release Cycle.
R-CEL-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Protein family/group databases

TCDBi2.A.23.2.10. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter
Alternative name(s):
Sodium-dependent glutamate/ aspartate transporter
Gene namesi
Name:glt-1
ORF Names:C12D12.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC12D12.2a; CE29083; WBGene00001620; glt-1.
C12D12.2b; CE29084; WBGene00001620; glt-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence analysisAdd
BLAST
Transmembranei19 – 3921HelicalSequence analysisAdd
BLAST
Transmembranei59 – 7921HelicalSequence analysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 19882ExtracellularSequence analysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST
Transmembranei281 – 30121HelicalSequence analysisAdd
BLAST
Transmembranei369 – 38921HelicalSequence analysisAdd
BLAST
Transmembranei400 – 42021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell projection Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Excitatory amino acid transporterPRO_0000202074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi177 – 1771N-linked (GlcNAc...)1 Publication
Glycosylationi187 – 1871N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ10901.
PaxDbiQ10901.
PRIDEiQ10901.

PTM databases

iPTMnetiQ10901.

Interactioni

Protein-protein interaction databases

STRINGi6239.C12D12.2a.1.

Structurei

3D structure databases

ProteinModelPortaliQ10901.
SMRiQ10901. Positions 29-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
InParanoidiQ10901.
KOiK05613.
OMAiITFNCLE.
PhylomeDBiQ10901.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q10901-1) [UniParc]FASTAAdd to basket

Also known as: Glt-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPDTRINKE IMVSWIRKNL LLVLTVSSVV LGALCGFLLR GLQLSPQNIM
60 70 80 90 100
YISFPGELLM HMLKMMILPL IMSSLISGLA QLDARQSGKL GSLAVTYYMF
110 120 130 140 150
TTAVAVVTGI FLVLVIHPGD PTIKKEIGTG TEGKTVSTVD TLLDLLRNMF
160 170 180 190 200
PENVVQATFQ QVQTKYIKVR PKVVKNNDSA TLAALNNGSL DYVKASVEYT
210 220 230 240 250
SGMNVLGVIV FCIAIGISLS QLGQEAHVMV QFFVIMDKVI MKLVMTVMWY
260 270 280 290 300
SPFGIFCLIM GKILEIHDLA DTARMLAMYM VTVLSGLAIH SLISLPLIFF
310 320 330 340 350
VTTKKNPYVF MRGLFQAWIT ALGTASSSAT LPITFNCLEE NLGVDRRVTR
360 370 380 390 400
FVLPVGATIN MDGTALYEAV AAIFIAQING VHLSFGQVVT VSLTATLASI
410 420 430 440 450
GAASVPSAGL VTMLLVLTAV GLPVKDVSLI VAVDWLLDRI RTSINVLGDA
460 470 480 490 500
MGAGIVYHYS KADLDAHDRL AATTRSHSIA MNDEKRQLAV YNSLPTDDEK

HTH
Length:503
Mass (Da):54,676
Last modified:July 15, 1998 - v2
Checksum:i3014BFF28E41E798
GO
Isoform b (identifier: Q10901-2) [UniParc]FASTAAdd to basket

Also known as: Glt-1

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Show »
Length:492
Mass (Da):53,380
Checksum:i5A4A53894038B1EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → P in AAB41909 (PubMed:8885221).Curated
Sequence conflicti68 – 681L → P in AAB41910 (PubMed:8885221).Curated
Sequence conflicti270 – 2734ADTA → ENTT in AAB41909 (PubMed:8885221).Curated
Sequence conflicti270 – 2734ADTA → ENTT in AAB41910 (PubMed:8885221).Curated
Sequence conflicti321 – 3211A → G in AAB41909 (PubMed:8885221).Curated
Sequence conflicti321 – 3211A → G in AAB41910 (PubMed:8885221).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1111Missing in isoform b. 1 PublicationVSP_006267Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35250 mRNA. Translation: AAB41909.1.
U35250 mRNA. Translation: AAB41910.1.
D86740 mRNA. Translation: BAA13164.1.
D86741 mRNA. Translation: BAA21840.1.
FO080514 Genomic DNA. Translation: CCD64307.1.
FO080514 Genomic DNA. Translation: CCD64308.1.
PIRiJC5078.
RefSeqiNP_001024393.1. NM_001029222.3. [Q10901-1]
NP_001024394.1. NM_001029223.2. [Q10901-2]
UniGeneiCel.21360.

Genome annotation databases

EnsemblMetazoaiC12D12.2a; C12D12.2a; WBGene00001620. [Q10901-1]
GeneIDi180641.
KEGGicel:CELE_C12D12.2.
UCSCiC12D12.2b.3. c. elegans. [Q10901-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35250 mRNA. Translation: AAB41909.1.
U35250 mRNA. Translation: AAB41910.1.
D86740 mRNA. Translation: BAA13164.1.
D86741 mRNA. Translation: BAA21840.1.
FO080514 Genomic DNA. Translation: CCD64307.1.
FO080514 Genomic DNA. Translation: CCD64308.1.
PIRiJC5078.
RefSeqiNP_001024393.1. NM_001029222.3. [Q10901-1]
NP_001024394.1. NM_001029223.2. [Q10901-2]
UniGeneiCel.21360.

3D structure databases

ProteinModelPortaliQ10901.
SMRiQ10901. Positions 29-461.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C12D12.2a.1.

Protein family/group databases

TCDBi2.A.23.2.10. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

iPTMnetiQ10901.

Proteomic databases

EPDiQ10901.
PaxDbiQ10901.
PRIDEiQ10901.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC12D12.2a; C12D12.2a; WBGene00001620. [Q10901-1]
GeneIDi180641.
KEGGicel:CELE_C12D12.2.
UCSCiC12D12.2b.3. c. elegans. [Q10901-1]

Organism-specific databases

CTDi180641.
WormBaseiC12D12.2a; CE29083; WBGene00001620; glt-1.
C12D12.2b; CE29084; WBGene00001620; glt-1.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
InParanoidiQ10901.
KOiK05613.
OMAiITFNCLE.
PhylomeDBiQ10901.

Enzyme and pathway databases

ReactomeiR-CEL-210500. Glutamate Neurotransmitter Release Cycle.
R-CEL-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

PROiQ10901.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of cDNAs encoding putative glutamate transporters from Caenorhabditis elegans and Onchocerca volvulus."
    Radice A.D., Lustigman S.
    Mol. Biochem. Parasitol. 80:41-53(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
    Strain: Bristol N2.
  2. "Molecular cloning of a cDNA for the glutamate transporter of the nematode Caenorhabditis elegans."
    Kawano T., Takuwa K., Nakajima T.
    Biochem. Biophys. Res. Commun. 228:415-420(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Bristol N2.
  3. "Structure and activity of a new form of the glutamate transporter of the nematode Caenorhabditis elegans."
    Kawano T., Takuwa K., Nakajima T.
    Biosci. Biotechnol. Biochem. 61:927-929(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Bristol N2.
  4. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  5. "Identification of the hydrophobic glycoproteins of Caenorhabditis elegans."
    Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J.
    Glycobiology 15:952-964(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-177 AND ASN-187, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEAA1_CAEEL
AccessioniPrimary (citable) accession number: Q10901
Secondary accession number(s): P90798, Q17920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: July 6, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.