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Q10836

- TRHDE_RAT

UniProt

Q10836 - TRHDE_RAT

Protein

Thyrotropin-releasing hormone-degrading ectoenzyme

Gene

Trhde

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Specific inactivation of TRH after its release.

    Catalytic activityi

    Release of the N-terminal pyroglutamyl group from pGlu-|-His-Xaa tripeptides and pGlu-|-His-Xaa-Gly tetrapeptides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi441 – 4411Zinc; catalyticPROSITE-ProRule annotation
    Active sitei442 – 4421Proton acceptorPROSITE-ProRule annotation
    Metal bindingi445 – 4451Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi464 – 4641Zinc; catalyticPROSITE-ProRule annotation
    Sitei528 – 5281Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: RGD
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: RGD

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thyrotropin-releasing hormone-degrading ectoenzyme (EC:3.4.19.6)
    Short name:
    TRH-DE
    Short name:
    TRH-degrading ectoenzyme
    Alternative name(s):
    Pyroglutamyl-peptidase II
    Short name:
    PAP-II
    TRH-specific aminopeptidase
    Thyroliberinase
    Gene namesi
    Name:Trhde
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi728895. Trhde.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10251025Thyrotropin-releasing hormone-degrading ectoenzymePRO_0000095120Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Phosphothreonine; by PKCSequence Analysis
    Disulfide bondi68 – 68Interchain
    Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi339 – 3391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi606 – 6061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi635 – 6351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi650 – 6501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi664 – 6641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi685 – 6851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi907 – 9071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ10836.
    PRIDEiQ10836.

    PTM databases

    PhosphoSiteiQ10836.

    Expressioni

    Tissue specificityi

    Predominantly expressed in brain and pituitary. Lower levels in lung and liver.

    Gene expression databases

    GenevestigatoriQ10836.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000007461.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10836.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4040CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini62 – 1025964ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei41 – 6121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni405 – 4095Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0308.
    HOGENOMiHOG000106482.
    HOVERGENiHBG095698.
    InParanoidiQ10836.
    PhylomeDBiQ10836.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    IPR015570. TRH-DE.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PTHR11533:SF40. PTHR11533:SF40. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q10836-1 [UniParc]FASTAAdd to Basket

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    MGEDDAALRA SGRGLSDPWA DSVGVRPRTT ERHIAVHKRL VLAFAVSIVA     50
    LLAVTMLAVL LSLRFDECGA SAAMPGTDGG LGGFPERGGN SSYPGSARRN 100
    HHAGEESSQR EIGEVGTAGT PSAHPPSEEE QEQWQPWTQL RLSGHLKPLH 150
    YNLMLTAFME NFTFSGEVNV EIACQNATRY VVLHASRVAV EKVQVAEDRA 200
    FGAVPVAGFF LYPQTQVLVV VLNRTLDAQR HYNLKIIYNA LIENELLGFF 250
    RSSYVIHGER RFLGVTQFSP THARKAFPCF DEPIYKATFK ISIKHQATYL 300
    SLSNMPVETS VFEEDGWVTD HFSQTPLMST YYLAWAICNF TYRETTTKSG 350
    VVVRLYARPD AIRRGSGDYA LHITKRLIEF YEDYFKVPYS LPKLDLLAVP 400
    KHPYAAMENW GLSIFVEQRI LLDPSVSSIS YLLDVTMVIV HEICHQWFGD 450
    LVTPVWWEDV WLKEGFAHYF EFVGTDYLYP SWNMEKQRFL TDVLHEVMLL 500
    DGLASSHPVS QEVLRATDID KVFDWIAYKK GAALIRMLAN FMGHSVFQRG 550
    LQDYLTIHKY GNAARNDLWN TLSEALKRNG KYVNIQEVMD QWTLQMGYPV 600
    ITILGNMTAE NRILITQQHF IYDIGAKTKA LQLQNSSYLW QIPLTIVVGN 650
    RSHVSSEAII WVSNKSEHHR ITYLDKGSWI LGNINQTGYF RVNYDLRNWR 700
    LLIDQLIRNH EVLSVSNRAG LIDDAFSLAR AGYLPQNIPL EIIRYLSEEK 750
    DFLPWHAASR ALYPLDKLLD RMENYNIFNE YILKQVATTY SKLGWPKNNF 800
    NGSVVQASYQ HEELRREVIM LACSFGNKHC HQQASTLISD WISSNRNRIP 850
    LNVRDIVYCT GVSLLDEDVW EFIWMKFHST TAVSEKKILL EALTCSDDRN 900
    LLSRLLNLSL NSEVVLDQDA IDVIIHVARN PHGRDLAWKF FRDKWKILNT 950
    RYGEALFMNS KLISGVTEFL NTEGELKELK NFMKSYDGVA SASFSRAVET 1000
    VEANVRWKRL YQDELFQWLG KAMRH 1025
    Length:1,025
    Mass (Da):117,287
    Last modified:November 1, 1996 - v1
    Checksum:i4024EB262608D16B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80535 mRNA. Translation: CAA56675.1.
    PIRiI59331.
    UniGeneiRn.23393.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80535 mRNA. Translation: CAA56675.1 .
    PIRi I59331.
    UniGenei Rn.23393.

    3D structure databases

    ProteinModelPortali Q10836.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000007461.

    Protein family/group databases

    MEROPSi M01.008.

    PTM databases

    PhosphoSitei Q10836.

    Proteomic databases

    PaxDbi Q10836.
    PRIDEi Q10836.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 728895. Trhde.

    Phylogenomic databases

    eggNOGi COG0308.
    HOGENOMi HOG000106482.
    HOVERGENi HBG095698.
    InParanoidi Q10836.
    PhylomeDBi Q10836.

    Miscellaneous databases

    PROi Q10836.

    Gene expression databases

    Genevestigatori Q10836.

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    IPR015570. TRH-DE.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    PTHR11533:SF40. PTHR11533:SF40. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding an ectoenzyme that degrades thyrotropin-releasing hormone."
      Schauder B., Schomburg L., Koehrle J., Bauer K.
      Proc. Natl. Acad. Sci. U.S.A. 91:9534-9538(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Sprague-Dawley.
      Tissue: Pituitary.
    2. "Analysis of the thyrotropin-releasing hormone-degrading ectoenzyme by site-directed mutagenesis of cysteine residues. Cys68 is involved in disulfide-linked dimerization."
      Papadopoulos T., Heuer H., Bauer K.
      Eur. J. Biochem. 267:2617-2623(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERCHAIN DISULFIDE BOND.

    Entry informationi

    Entry nameiTRHDE_RAT
    AccessioniPrimary (citable) accession number: Q10836
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3