Q10793 (RNH2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Rv2902c, MT2970
ORF Names:	MTCY274.33c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00052_B
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Ribonuclease HII HAMAP MF_00052_B		PRO_0000111592
Sites
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	40	1	Divalent metal cation By similarity
	Metal binding	133	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10793 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 786F0E790188279F	FASTA	264	27,646
	10 20 30 40 50 60 MTKTWPPRTV IRKSGGLRGM RTLESALHRG GLGPVAGVDE VGRGACAGPL VVAACVLGPG 70 80 90 100 110 120 RIASLAALDD SKKLSEQARE KLFPLICRYA VAYHVVFIPS AEVDRRGVHV ANIEGMRRAV 130 140 150 160 170 180 AGLAVRPGYV LSDGFRVPGL PMPSLPVIGG DAAAACIAAA SVLAKVSRDR VMVALDADHP 190 200 210 220 230 240 GYGFAEHKGY STPAHSRALA RLGPCPQHRY SFINVRRVAS GSNTAEVADG QPDPRDGTAQ 250 260 TGEGRWSKSS HPATMRATGR AQGT

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842581 Genomic DNA. Translation: CAA98340.1. AE000516 Genomic DNA. Translation: AAK47296.1.
PIR	A70927.
RefSeq	NP_217418.1. NC_000962.2. NP_337482.1. NC_002755.2.
3D structure databases
ProteinModelPortal	Q10793.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000002732; EBMYCP00000002732; EBMYCG00000002730. EBMYCT00000069717; EBMYCP00000067776; EBMYCG00000069712.
GeneID	888504. 925308.
GenomeReviews	Gene locus MT2970 in contig AE000516_GR. Gene locus Rv2902c in contig AL123456_GR.
KEGG	mtc:MT2970. mtu:Rv2902c.
PATRIC	18128300. VBIMycTub22151_3249.
TIGR	MT2970.
Organism-specific databases
TubercuList	Rv2902c.
Phylogenomic databases
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	Q10793.
ProtClustDB	PRK00015.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCTU

Accession

Primary (citable) accession number: Q10793

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program