ID   LEP_MYCTU               Reviewed;         294 AA.
AC   Q10789;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 69.
DE   RecName: Full=Probable signal peptidase I;
DE            Short=SPase I;
DE            EC=3.4.21.89;
DE   AltName: Full=Leader peptidase I;
GN   Name=lepB; OrderedLocusNames=Rv2903c, MT2971; ORFNames=MTCY274.34c;
OS   Mycobacterium tuberculosis.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=1773;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   MEDLINE=98295987; PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M.,
RA   Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III,
RA   Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
RA   Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S.,
RA   Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A.,
RA   Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R.,
RA   Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the
RT   complete genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   MEDLINE=22206494; PubMed=12218036;
RX   DOI=10.1128/JB.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H.,
RA   Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D.,
RA   Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M.,
RA   Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C.,
RA   Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY: Cleavage of hydrophobic, N-terminal signal or
CC       leader sequences from secreted and periplasmic proteins.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane
CC       protein (By similarity).
CC   -!- SIMILARITY: Belongs to the peptidase S26 family.
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DR   EMBL; BX842581; CAA98341.1; -; Genomic_DNA.
DR   EMBL; AE000516; AAK47297.1; -; Genomic_DNA.
DR   PIR; B70927; B70927.
DR   RefSeq; NP_217419.1; -.
DR   RefSeq; NP_337483.1; -.
DR   HSSP; P00803; 1B12.
DR   GeneID; 887157; -.
DR   GeneID; 925307; -.
DR   GenomeReviews; AE000516_GR; MT2971.
DR   GenomeReviews; AL123456_GR; Rv2903c.
DR   KEGG; mtc:MT2971; -.
DR   KEGG; mtu:Rv2903c; -.
DR   TIGR; MT2971; -.
DR   TubercuList; Rv2903c; -.
DR   HOGENOM; Q10789; -.
DR   OMA; Q10789; VDKLTYR.
DR   BRENDA; 3.4.21.89; 809.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR   InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR   InterPro; IPR019757; Pept_S26A_signal_pept_1_Lys-AS.
DR   InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR   InterPro; IPR019759; Peptidase_S24_S26_cons-reg.
DR   InterPro; IPR011056; Peptidase_S24_S26A/B/C_b-rbn.
DR   Gene3D; G3DSA:2.10.109.10; Pept_S24_S26_C; 1.
DR   Pfam; PF00717; Peptidase_S24; 1.
DR   PRINTS; PR00727; LEADERPTASE.
DR   TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR   PROSITE; PS00501; SPASE_I_1; 1.
DR   PROSITE; PS00760; SPASE_I_2; FALSE_NEG.
DR   PROSITE; PS00761; SPASE_I_3; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Complete proteome; Hydrolase; Membrane; Protease;
KW   Transmembrane.
FT   CHAIN         1    294       Probable signal peptidase I.
FT                                /FTId=PRO_0000109511.
FT   TOPO_DOM      1     66       Cytoplasmic (Potential).
FT   TRANSMEM     67     87       Potential.
FT   TOPO_DOM     88    294       Extracellular (Potential).
FT   ACT_SITE     96     96       By similarity.
FT   ACT_SITE    174    174       By similarity.
SQ   SEQUENCE   294 AA;  31880 MW;  BBCAE8ADF68F897D CRC64;
     MTETTDSPSE RQPGPAEPEL SSRDPDIAGQ VFDAAPFDAA PDADSEGDSK AAKTDEPRPA
     KRSTLREFAV LAVIAVVLYY VMLTFVARPY LIPSESMEPT LHGCSTCVGD RIMVDKLSYR
     FGSPQPGDVI VFRGPPSWNV GYKSIRSHNV AVRWVQNALS FIGFVPPDEN DLVKRVIAVG
     GQTVQCRSDT GLTVNGRPLK EPYLDPATMM ADPSIYPCLG SEFGPVTVPP GRVWVMGDNR
     THSADSRAHC PLLCTDDPLP GTVPVANVIG KARLIVWPPS RWGVVRSVNP QQGR
//