Q10789 (LEP_MYCTU) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 100.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Signal peptidase I Short name=SPase I EC=3.4.21.89 Alternative name(s): Leader peptidase I | ||||||
| Gene names |
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| Organism | Mycobacterium tuberculosis [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1773 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex![]() |
Protein attributes
| Sequence length | 294 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins. Ref.4 |
| Enzyme regulation | Inhibited by 1-(2,5-dichlorophenyl)-3-(dimethylamino)propan-1-one (MD3). Ref.4 |
| Subcellular location | Cell membrane; Single-pass type II membrane protein By similarity. |
| Miscellaneous | Was identified as a high-confidence drug target. |
| Sequence similarities | Belongs to the peptidase S26 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Hydrolase Protease |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | extracellular region Inferred from genetic interaction PubMed 10781540. Source: MTBBASE integral to plasma membraneInferred from direct assay PubMed 15952732. Source: MTBBASE |
| Molecular_function | serine-type peptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 294 | 294 | Signal peptidase I | PRO_0000109511 | |||||
Regions | |||||||||
| Topological domain | 1 – 66 | 66 | Cytoplasmic Potential | ||||||
| Transmembrane | 67 – 87 | 21 | Helical; Potential | ||||||
| Topological domain | 88 – 294 | 207 | Extracellular Potential | ||||||
Sites | |||||||||
| Active site | 96 | 1 | Probable | ||||||
| Active site | 174 | 1 | Probable | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G.Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv. |
| [2] | "Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M.J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh. |
| [3] | "targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS]. |
| [4] | "Inhibition of the sole type I signal peptidase of Mycobacterium tuberculosis is bactericidal under replicating and nonreplicating conditions." Ollinger J., O'Malley T., Ahn J., Odingo J., Parish T. J. Bacteriol. 194:2614-2619(2012) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BX842581 Genomic DNA. Translation: CAA98341.1. AE000516 Genomic DNA. Translation: AAK47297.1. AL123456 Genomic DNA. Translation: CCP45705.1. |
| PIR | B70927. |
| RefSeq | NP_217419.1. NC_000962.3. NP_337483.1. NC_002755.2. YP_006516356.1. NC_018143.1. |
3D structure databases | |
| ProteinModelPortal | Q10789. |
| SMR | Q10789. Positions 84-285. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 83332.Rv2903c. |
Protein family/group databases | |
| MEROPS | S26.024. |
Proteomic databases | |
| PRIDE | Q10789. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAK47297; AAK47297; MT2971. |
| GeneID | 13317696. 887157. 925307. |
| KEGG | mtc:MT2971. mtu:Rv2903c. mtv:RVBD_2903c. |
| PATRIC | 18128302. VBIMycTub22151_3250. |
Organism-specific databases | |
| TubercuList | Rv2903c. |
Phylogenomic databases | |
| eggNOG | COG0681. |
| HOGENOM | HOG000003673. |
| KO | K03100. |
| OMA | TVECCDD. |
| ProtClustDB | CLSK792152. |
Family and domain databases | |
| Gene3D | 2.10.109.10. 2 hits. |
| InterPro | IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view] |
| PANTHER | PTHR12383. PTHR12383. 1 hit. |
| Pfam | PF00717. Peptidase_S24. 1 hit. [Graphical view] |
| PRINTS | PR00727. LEADERPTASE. |
| SUPFAM | SSF51306. Pept_S24_S26_C. 1 hit. |
| TIGRFAMs | TIGR02227. sigpep_I_bact. 1 hit. |
| PROSITE | PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. False negative. PS00761. SPASE_I_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LEP_MYCTU | ||||||||
| Accession | Primary (citable) accession number: Q10789 Secondary accession number(s): L0TAZ5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
