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Reviewed, UniProtKB/Swiss-Prot Q10789 (LEP_MYCTU)

Last modified February 9, 2010. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I
Gene names
Name: lepB
Ordered Locus Names: Rv2903c, MT2971
ORF Names: MTCY274.34c
OrganismMycobacterium tuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Subcellular location

Cell membrane; Single-pass type II membrane protein By similarity.

Miscellaneous

Was identified as a high-confidence drug target.

Sequence similarities

Belongs to the peptidase S26 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   Molecular functionHydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionserine-type peptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Probable signal peptidase I
PRO_0000109511

Regions

Topological domain1 – 6666Cytoplasmic Potential
Transmembrane67 – 8721 Potential
Topological domain88 – 294207Extracellular Potential

Sites

Active site961 By similarity
Active site1741 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q10789-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BBCAE8ADF68F897D

FASTA29431,880
        10         20         30         40         50         60 
MTETTDSPSE RQPGPAEPEL SSRDPDIAGQ VFDAAPFDAA PDADSEGDSK AAKTDEPRPA 

        70         80         90        100        110        120 
KRSTLREFAV LAVIAVVLYY VMLTFVARPY LIPSESMEPT LHGCSTCVGD RIMVDKLSYR 

       130        140        150        160        170        180 
FGSPQPGDVI VFRGPPSWNV GYKSIRSHNV AVRWVQNALS FIGFVPPDEN DLVKRVIAVG 

       190        200        210        220        230        240 
GQTVQCRSDT GLTVNGRPLK EPYLDPATMM ADPSIYPCLG SEFGPVTVPP GRVWVMGDNR 

       250        260        270        280        290 
THSADSRAHC PLLCTDDPLP GTVPVANVIG KARLIVWPPS RWGVVRSVNP QQGR 

« Hide

References

[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis."
Raman K., Yeturu K., Chandra N.
BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract]
Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX842581 Genomic DNA. Translation: CAA98341.1.
AE000516 Genomic DNA. Translation: AAK47297.1.
PIRB70927.
RefSeqNP_217419.1.
NP_337483.1.

3D structure databases

SMRQ10789. Positions 84-287.
ModBaseSearch...

Genome annotation databases

GeneID887157.
925307.
GenomeReviewsGene locus MT2971 in contig AE000516_GR.
KEGGmtc:MT2971.
mtu:Rv2903c.
TIGRMT2971.

Organism-specific databases

TubercuListRv2903c.

Phylogenomic databases

HOGENOMHBG596607.
OMAQTVECCD.

Enzyme and pathway databases

BRENDA3.4.21.89. 809.

Family and domain databases

InterProIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR019759. Peptidase_S24_S26_cons-reg.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011056. Peptidase_S24_S26A/B/C_b-rbn.
[Graphical view]
Gene3DG3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
PfamPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSPR00727. LEADERPTASE.
TIGRFAMsTIGR02227. sigpep_I_bact. 1 hit.
PROSITEPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. False negative.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEP_MYCTU
AccessionPrimary (citable) accession number: Q10789
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents