Q10789 (LEP_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	Rv2903c, MT2971
ORF Names:	MTCY274.34c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins. Ref.4
Enzyme regulation	Inhibited by 1-(2,5-dichlorophenyl)-3-(dimethylamino)propan-1-one (MD3). Ref.4
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from genetic interaction PubMed 10781540. Source: MTBBASE integral to plasma membrane Inferred from direct assay PubMed 15952732. Source: MTBBASE
Molecular_function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Signal peptidase I		PRO_0000109511
Regions
	Topological domain	1 – 66	66	Cytoplasmic Potential
	Transmembrane	67 – 87	21	Helical; Potential
	Topological domain	88 – 294	207	Extracellular Potential
Sites
	Active site	96	1	Probable
	Active site	174	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	Q10789 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BBCAE8ADF68F897D	FASTA	294	31,880
	10 20 30 40 50 60 MTETTDSPSE RQPGPAEPEL SSRDPDIAGQ VFDAAPFDAA PDADSEGDSK AAKTDEPRPA 70 80 90 100 110 120 KRSTLREFAV LAVIAVVLYY VMLTFVARPY LIPSESMEPT LHGCSTCVGD RIMVDKLSYR 130 140 150 160 170 180 FGSPQPGDVI VFRGPPSWNV GYKSIRSHNV AVRWVQNALS FIGFVPPDEN DLVKRVIAVG 190 200 210 220 230 240 GQTVQCRSDT GLTVNGRPLK EPYLDPATMM ADPSIYPCLG SEFGPVTVPP GRVWVMGDNR 250 260 270 280 290 THSADSRAHC PLLCTDDPLP GTVPVANVIG KARLIVWPPS RWGVVRSVNP QQGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[4]	"Inhibition of the sole type I signal peptidase of Mycobacterium tuberculosis is bactericidal under replicating and nonreplicating conditions." Ollinger J., O'Malley T., Ahn J., Odingo J., Parish T. J. Bacteriol. 194:2614-2619(2012) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842581 Genomic DNA. Translation: CAA98341.1. AE000516 Genomic DNA. Translation: AAK47297.1. AL123456 Genomic DNA. Translation: CCP45705.1.
PIR	B70927.
RefSeq	NP_217419.1. NC_000962.3. NP_337483.1. NC_002755.2. YP_006516356.1. NC_018143.1.
3D structure databases
ProteinModelPortal	Q10789.
SMR	Q10789. Positions 84-285.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv2903c.
Protein family/group databases
MEROPS	S26.024.
Proteomic databases
PRIDE	Q10789.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK47297; AAK47297; MT2971.
GeneID	13317696. 887157. 925307.
KEGG	mtc:MT2971. mtu:Rv2903c. mtv:RVBD_2903c.
PATRIC	18128302. VBIMycTub22151_3250.
Organism-specific databases
TubercuList	Rv2903c.
Phylogenomic databases
eggNOG	COG0681.
HOGENOM	HOG000003673.
KO	K03100.
OMA	TVECCDD.
ProtClustDB	CLSK792152.
Family and domain databases
Gene3D	2.10.109.10. 2 hits.
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
PANTHER	PTHR12383. PTHR12383. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. False negative. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_MYCTU

Accession

Primary (citable) accession number: Q10789
Secondary accession number(s): L0TAZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program