Q10788 (EFTS_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Ts
Short name=EF-Ts

Gene names

Name:	tsf
Ordered Locus Names:	Rv2889c, MT2957
ORF Names:	MTCY274.20c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. HAMAP MF_00050
Subcellular location	Cytoplasm HAMAP MF_00050.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00050
Sequence similarities	Belongs to the EF-Ts family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Elongation factor Ts HAMAP MF_00050		PRO_0000161160
Regions
	Region	76 – 79	4	Involved in Mg(2+) ion dislocation from EF-Tu By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10788 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 23064C8C2D95B0A2	FASTA	271	28,755
	10 20 30 40 50 60 MANFTAADVK RLRELTGAGM LACKNALAET DGDFDKAVEA LRIKGAKDVG KRAERATAEG 70 80 90 100 110 120 LVAAKDGALI ELNCETDFVA KNAEFQTLAD QVVAAAAAAK PADVDALKGA SIGDKTVEQA 130 140 150 160 170 180 IAELSAKIGE KLELRRVAIF DGTVEAYLHR RSADLPPAVG VLVEYRGDDA AAAHAVALQI 190 200 210 220 230 240 AALRARYLSR DDVPEDIVAS ERRIAEETAR AEGKPEQALP KIVEGRLNGF FKDAVLLEQA 250 260 270 SVSDNKKTVK ALLDVAGVTV TRFVRFEVGQ A

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842581 Genomic DNA. Translation: CAA98365.1. AE000516 Genomic DNA. Translation: AAK47282.1.
PIR	D70925.
RefSeq	NP_217405.1. NC_000962.2. NP_337468.1. NC_002755.2.
3D structure databases
ProteinModelPortal	Q10788.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001377; EBMYCP00000001377; EBMYCG00000001377. EBMYCT00000071329; EBMYCP00000069388; EBMYCG00000071324.
GeneID	888187. 925323.
GenomeReviews	Gene locus MT2957 in contig AE000516_GR. Gene locus Rv2889c in contig AL123456_GR.
KEGG	mtc:MT2957. mtu:Rv2889c.
PATRIC	18128272. VBIMycTub22151_3235.
TIGR	MT2957.
Organism-specific databases
TubercuList	Rv2889c.
Phylogenomic databases
GeneTree	EBGT00050000016347.
HOGENOM	HBG713289.
OMA	YLHGTRI.
PhylomeDB	Q10788.
ProtClustDB	PRK09377.
Family and domain databases
HAMAP	MF_00050. EF_Ts. [Tree]
InterPro	IPR001816. Transl_elong_EFTs/EF1B. IPR014039. Transl_elong_EFTs/EF1B_dimer. IPR018101. Transl_elong_Ts_CS. IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. [Graphical view]
Gene3D	G3DSA:3.30.479.20. Transl_elong_EFTs/EF1B_dimer. 3 hits.
KO	K02357.
PANTHER	PTHR11741. Transl_elong_EFTs/EF1B. 1 hit.
Pfam	PF00889. EF_TS. 1 hit. PF00627. UBA. 1 hit. [Graphical view]
SUPFAM	SSF54713. EF_TS. 1 hit. SSF46934. UBA_like. 1 hit.
TIGRFAMs	TIGR00116. Tsf. 1 hit.
PROSITE	PS01126. EF_TS_1. 1 hit. PS01127. EF_TS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTS_MYCTU

Accession

Primary (citable) accession number: Q10788

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program