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Q10752

- CDC28_SCHPO

UniProt

Q10752 - CDC28_SCHPO

Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28

Gene

cdc28

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (29 Aug 2001)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing. Is required together with ATP and at least one other factor, for the first cleavage-ligation reaction. Functions as a molecular motor in the activation of the precatalytic spliceosome for the first transesterification reaction of pre-mRNA splicing by hydrolyzing ATP to cause the activation of the spliceosome without the occurrence of splicing By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi441 – 4488ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: PomBase
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. mRNA cis splicing, via spliceosome Source: PomBase

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28 (EC:3.6.4.13)
    Alternative name(s):
    Pre-mRNA-processing protein 8
    Gene namesi
    Name:cdc28
    Synonyms:prp8
    ORF Names:SPBC19C2.01, SPBC21B10.01c, SPBC874.01
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC19C2.01.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. spliceosomal complex Source: PomBase
    2. U4/U6 x U5 tri-snRNP complex Source: PomBase
    3. U5 snRNP Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Cells show pre-mRNA splicing defects. Cdc28-P8 temperature-sensitive mutant causes cell-cycle arrest in G2 and exhibits a splicing defect that leads to accumulation of unspliced precursors at the restrictive temperature. Temperature-sensitive pre-mRNA splicing mutant Prp8-1 exhibits a cell-cycle phenotype identical to cdc28-p8. Prp8-1 mutant produces elongated cells, accumulates U6 snRNA precursor and has defects in an early step of TFIID pre-mRNA splicing at the nonpermissive temperature.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10551055Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28PRO_0000055154Add
    BLAST

    Proteomic databases

    MaxQBiQ10752.

    Interactioni

    Protein-protein interaction databases

    BioGridi277230. 11 interactions.
    STRINGi4896.SPBC19C2.01-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10752.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini428 – 592165Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini617 – 790174Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi539 – 5424DEAH box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    HOGENOMiHOG000175261.
    KOiK12813.
    OMAiIEIESKW.
    OrthoDBiEOG7M98QW.
    PhylomeDBiQ10752.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q10752-1 [UniParc]FASTAAdd to Basket

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    MSLEQYVSDK AISLLGMSEP SVVEYLIAEA KGSSSSNNLY QKLVSFGMDG     50
    DDPAVKEFAH TLYARIPREG SRPKENYNAR KKKEQGILQM ERLNSSYDLL 100
    IEPQSHETPG KPLKKKSRSK TPKREIARRQ RDEDEWESDE YEEVVDGSAS 150
    HPIEEDSVST DFQNHDYEKS SDPETERLND LREREEFEER LRRKDLEAAT 200
    NEFVEDYSSK FSSEELALRK LADDPESWRK LASELRKKSR QQYLKPRAQQ 250
    QLEILRREIR DEEQLFAGEK LTQAEIRELE KKKELLRIAE ERQRLEKQAT 300
    EYQMPEDYFT EQGKLDRKRK EEVLYQRYKD SNEGEQNEVT MGAAEQQRWE 350
    AQQINKALLF DQNEWLPPGE KQFDFVFDES QQIDFLLDTK LSAENPVDTD 400
    KMTDVKVEKS LESSRKSLPV YQYKDDLLKA INEYQVLLIV AETGSGKTTQ 450
    LPQFLHEAGY TKGNKKICCT QPRRVAAMSV AARVAKEMDV RLGQEVGYSI 500
    RFENATSEKT VIKYLTDGML LREFLTEPDL ASYSVIIIDE AHERTLHTDI 550
    LFGLVKDIAR FRPDLKVLIS SATIDAEKFS AYFDEAPVFY VPGRRYPVDI 600
    YYTPQPEANY IQAAITTILQ IHTTQPAGDI LVFLTGQDEI ELMSENMQEL 650
    CRILGKRIPE IILCPIYANL PSELQAKIFD PTPPGARKVV LATNIAETSI 700
    TIDGVNFVID SGFVKQNMYN PRTGMESLVS VPCSRASADQ RAGRAGRVGP 750
    GKCFRLYTRR TYNNELDMVT SPEIQRTNLT NIVLLLKSLG INNLLDFDFM 800
    DAPPPETLMR SLELLYALGA LNNRGELTKL GRQMAEFPTD PMLSKSLIAS 850
    SKYGCVEEVL SIVSMLGEAS SLFYRPKDKI MEADKARANF TQPGGDHLTL 900
    LHIWNEWVDT DFSYNWAREN FLQYKSLCRA RDVRDQLANL CERVEIELVT 950
    NSSESLDPIK KAITAGYFSN AARLDRSGDS YRTVKSNQTV YIHPSSSVAE 1000
    KKPKVIIYFE LVLTTKEYCR QITEIQPEWL LEISPHYFKP ENIEELQKTQ 1050
    KRHKR 1055
    Length:1,055
    Mass (Da):121,217
    Last modified:August 29, 2001 - v2
    Checksum:i223694BD85404DCC
    GO

    Sequence cautioni

    The sequence AAC49377.1 differs from that shown. Reason: Frameshift at positions 80 and 1012.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti760 – 7601R → W in AAC49377. (PubMed:8862522)Curated
    Sequence conflicti969 – 9691S → P in AAC49377. (PubMed:8862522)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48733 Genomic DNA. Translation: AAC49377.1. Frameshift.
    CU329671 Genomic DNA. Translation: CAB57929.2.
    PIRiT46568.
    T50372.
    RefSeqiNP_595686.2. NM_001021581.3.

    Genome annotation databases

    EnsemblFungiiSPBC19C2.01.1; SPBC19C2.01.1:pep; SPBC19C2.01.
    GeneIDi2540707.
    KEGGispo:SPBC19C2.01.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48733 Genomic DNA. Translation: AAC49377.1 . Frameshift.
    CU329671 Genomic DNA. Translation: CAB57929.2 .
    PIRi T46568.
    T50372.
    RefSeqi NP_595686.2. NM_001021581.3.

    3D structure databases

    ProteinModelPortali Q10752.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277230. 11 interactions.
    STRINGi 4896.SPBC19C2.01-1.

    Proteomic databases

    MaxQBi Q10752.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC19C2.01.1 ; SPBC19C2.01.1:pep ; SPBC19C2.01 .
    GeneIDi 2540707.
    KEGGi spo:SPBC19C2.01.

    Organism-specific databases

    PomBasei SPBC19C2.01.

    Phylogenomic databases

    eggNOGi COG1643.
    HOGENOMi HOG000175261.
    KOi K12813.
    OMAi IEIESKW.
    OrthoDBi EOG7M98QW.
    PhylomeDBi Q10752.

    Miscellaneous databases

    NextBioi 20801829.
    PROi Q10752.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase."
      Lundgren K., Allan S., Urushiyama S., Tani T., Ohshima Y., Frendewey D., Beach D.
      Mol. Biol. Cell 7:1083-1094(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe."
      Urushiyama S., Tani T., Ohshima Y.
      Mol. Gen. Genet. 253:118-127(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCDC28_SCHPO
    AccessioniPrimary (citable) accession number: Q10752
    Secondary accession number(s): Q9URU1, Q9USV9, Q9UUD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 29, 2001
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3