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Q10752

- CDC28_SCHPO

UniProt

Q10752 - CDC28_SCHPO

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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28

Gene
cdc28, prp8, SPBC19C2.01, SPBC21B10.01c, SPBC874.01
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in pre-mRNA splicing. Is required together with ATP and at least one other factor, for the first cleavage-ligation reaction. Functions as a molecular motor in the activation of the precatalytic spliceosome for the first transesterification reaction of pre-mRNA splicing by hydrolyzing ATP to cause the activation of the spliceosome without the occurrence of splicing By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi441 – 4488ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent RNA helicase activity Source: PomBase
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. mRNA cis splicing, via spliceosome Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28 (EC:3.6.4.13)
Alternative name(s):
Pre-mRNA-processing protein 8
Gene namesi
Name:cdc28
Synonyms:prp8
ORF Names:SPBC19C2.01, SPBC21B10.01c, SPBC874.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC19C2.01.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. spliceosomal complex Source: PomBase
  2. U4/U6 x U5 tri-snRNP complex Source: PomBase
  3. U5 snRNP Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Cells show pre-mRNA splicing defects. Cdc28-P8 temperature-sensitive mutant causes cell-cycle arrest in G2 and exhibits a splicing defect that leads to accumulation of unspliced precursors at the restrictive temperature. Temperature-sensitive pre-mRNA splicing mutant Prp8-1 exhibits a cell-cycle phenotype identical to cdc28-p8. Prp8-1 mutant produces elongated cells, accumulates U6 snRNA precursor and has defects in an early step of TFIID pre-mRNA splicing at the nonpermissive temperature.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10551055Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28PRO_0000055154Add
BLAST

Proteomic databases

MaxQBiQ10752.

Interactioni

Protein-protein interaction databases

BioGridi277230. 11 interactions.
STRINGi4896.SPBC19C2.01-1.

Structurei

3D structure databases

ProteinModelPortaliQ10752.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini428 – 592165Helicase ATP-bindingAdd
BLAST
Domaini617 – 790174Helicase C-terminalAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi539 – 5424DEAH box

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1643.
HOGENOMiHOG000175261.
KOiK12813.
OMAiIEIESKW.
OrthoDBiEOG7M98QW.
PhylomeDBiQ10752.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10752-1 [UniParc]FASTAAdd to Basket

« Hide

MSLEQYVSDK AISLLGMSEP SVVEYLIAEA KGSSSSNNLY QKLVSFGMDG     50
DDPAVKEFAH TLYARIPREG SRPKENYNAR KKKEQGILQM ERLNSSYDLL 100
IEPQSHETPG KPLKKKSRSK TPKREIARRQ RDEDEWESDE YEEVVDGSAS 150
HPIEEDSVST DFQNHDYEKS SDPETERLND LREREEFEER LRRKDLEAAT 200
NEFVEDYSSK FSSEELALRK LADDPESWRK LASELRKKSR QQYLKPRAQQ 250
QLEILRREIR DEEQLFAGEK LTQAEIRELE KKKELLRIAE ERQRLEKQAT 300
EYQMPEDYFT EQGKLDRKRK EEVLYQRYKD SNEGEQNEVT MGAAEQQRWE 350
AQQINKALLF DQNEWLPPGE KQFDFVFDES QQIDFLLDTK LSAENPVDTD 400
KMTDVKVEKS LESSRKSLPV YQYKDDLLKA INEYQVLLIV AETGSGKTTQ 450
LPQFLHEAGY TKGNKKICCT QPRRVAAMSV AARVAKEMDV RLGQEVGYSI 500
RFENATSEKT VIKYLTDGML LREFLTEPDL ASYSVIIIDE AHERTLHTDI 550
LFGLVKDIAR FRPDLKVLIS SATIDAEKFS AYFDEAPVFY VPGRRYPVDI 600
YYTPQPEANY IQAAITTILQ IHTTQPAGDI LVFLTGQDEI ELMSENMQEL 650
CRILGKRIPE IILCPIYANL PSELQAKIFD PTPPGARKVV LATNIAETSI 700
TIDGVNFVID SGFVKQNMYN PRTGMESLVS VPCSRASADQ RAGRAGRVGP 750
GKCFRLYTRR TYNNELDMVT SPEIQRTNLT NIVLLLKSLG INNLLDFDFM 800
DAPPPETLMR SLELLYALGA LNNRGELTKL GRQMAEFPTD PMLSKSLIAS 850
SKYGCVEEVL SIVSMLGEAS SLFYRPKDKI MEADKARANF TQPGGDHLTL 900
LHIWNEWVDT DFSYNWAREN FLQYKSLCRA RDVRDQLANL CERVEIELVT 950
NSSESLDPIK KAITAGYFSN AARLDRSGDS YRTVKSNQTV YIHPSSSVAE 1000
KKPKVIIYFE LVLTTKEYCR QITEIQPEWL LEISPHYFKP ENIEELQKTQ 1050
KRHKR 1055
Length:1,055
Mass (Da):121,217
Last modified:August 29, 2001 - v2
Checksum:i223694BD85404DCC
GO

Sequence cautioni

The sequence AAC49377.1 differs from that shown. Reason: Frameshift at positions 80 and 1012.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti760 – 7601R → W in AAC49377. 1 Publication
Sequence conflicti969 – 9691S → P in AAC49377. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48733 Genomic DNA. Translation: AAC49377.1. Frameshift.
CU329671 Genomic DNA. Translation: CAB57929.2.
PIRiT46568.
T50372.
RefSeqiNP_595686.2. NM_001021581.3.

Genome annotation databases

EnsemblFungiiSPBC19C2.01.1; SPBC19C2.01.1:pep; SPBC19C2.01.
GeneIDi2540707.
KEGGispo:SPBC19C2.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48733 Genomic DNA. Translation: AAC49377.1 . Frameshift.
CU329671 Genomic DNA. Translation: CAB57929.2 .
PIRi T46568.
T50372.
RefSeqi NP_595686.2. NM_001021581.3.

3D structure databases

ProteinModelPortali Q10752.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277230. 11 interactions.
STRINGi 4896.SPBC19C2.01-1.

Proteomic databases

MaxQBi Q10752.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC19C2.01.1 ; SPBC19C2.01.1:pep ; SPBC19C2.01 .
GeneIDi 2540707.
KEGGi spo:SPBC19C2.01.

Organism-specific databases

PomBasei SPBC19C2.01.

Phylogenomic databases

eggNOGi COG1643.
HOGENOMi HOG000175261.
KOi K12813.
OMAi IEIESKW.
OrthoDBi EOG7M98QW.
PhylomeDBi Q10752.

Miscellaneous databases

NextBioi 20801829.
PROi Q10752.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase."
    Lundgren K., Allan S., Urushiyama S., Tani T., Ohshima Y., Frendewey D., Beach D.
    Mol. Biol. Cell 7:1083-1094(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe."
    Urushiyama S., Tani T., Ohshima Y.
    Mol. Gen. Genet. 253:118-127(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCDC28_SCHPO
AccessioniPrimary (citable) accession number: Q10752
Secondary accession number(s): Q9URU1, Q9USV9, Q9UUD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 29, 2001
Last modified: May 14, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi