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Q10752 (CDC28_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28

EC=3.6.4.13
Alternative name(s):
Pre-mRNA-processing protein 8
Gene names
Name:cdc28
Synonyms:prp8
ORF Names:SPBC19C2.01, SPBC21B10.01c, SPBC874.01
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length1055 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in pre-mRNA splicing. Is required together with ATP and at least one other factor, for the first cleavage-ligation reaction. Functions as a molecular motor in the activation of the precatalytic spliceosome for the first transesterification reaction of pre-mRNA splicing by hydrolyzing ATP to cause the activation of the spliceosome without the occurrence of splicing By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Nucleus By similarity.

Disruption phenotype

Cells show pre-mRNA splicing defects. Cdc28-P8 temperature-sensitive mutant causes cell-cycle arrest in G2 and exhibits a splicing defect that leads to accumulation of unspliced precursors at the restrictive temperature. Temperature-sensitive pre-mRNA splicing mutant Prp8-1 exhibits a cell-cycle phenotype identical to cdc28-p8. Prp8-1 mutant produces elongated cells, accumulates U6 snRNA precursor and has defects in an early step of TFIID pre-mRNA splicing at the nonpermissive temperature. Ref.1 Ref.3

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence AAC49377.1 differs from that shown. Reason: Frameshift at positions 80 and 1012.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10551055Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein cdc28
PRO_0000055154

Regions

Domain428 – 592165Helicase ATP-binding
Domain617 – 790174Helicase C-terminal
Nucleotide binding441 – 4488ATP By similarity
Motif539 – 5424DEAH box

Experimental info

Sequence conflict7601R → W in AAC49377. Ref.1
Sequence conflict9691S → P in AAC49377. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q10752 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 223694BD85404DCC

FASTA1,055121,217
        10         20         30         40         50         60 
MSLEQYVSDK AISLLGMSEP SVVEYLIAEA KGSSSSNNLY QKLVSFGMDG DDPAVKEFAH 

        70         80         90        100        110        120 
TLYARIPREG SRPKENYNAR KKKEQGILQM ERLNSSYDLL IEPQSHETPG KPLKKKSRSK 

       130        140        150        160        170        180 
TPKREIARRQ RDEDEWESDE YEEVVDGSAS HPIEEDSVST DFQNHDYEKS SDPETERLND 

       190        200        210        220        230        240 
LREREEFEER LRRKDLEAAT NEFVEDYSSK FSSEELALRK LADDPESWRK LASELRKKSR 

       250        260        270        280        290        300 
QQYLKPRAQQ QLEILRREIR DEEQLFAGEK LTQAEIRELE KKKELLRIAE ERQRLEKQAT 

       310        320        330        340        350        360 
EYQMPEDYFT EQGKLDRKRK EEVLYQRYKD SNEGEQNEVT MGAAEQQRWE AQQINKALLF 

       370        380        390        400        410        420 
DQNEWLPPGE KQFDFVFDES QQIDFLLDTK LSAENPVDTD KMTDVKVEKS LESSRKSLPV 

       430        440        450        460        470        480 
YQYKDDLLKA INEYQVLLIV AETGSGKTTQ LPQFLHEAGY TKGNKKICCT QPRRVAAMSV 

       490        500        510        520        530        540 
AARVAKEMDV RLGQEVGYSI RFENATSEKT VIKYLTDGML LREFLTEPDL ASYSVIIIDE 

       550        560        570        580        590        600 
AHERTLHTDI LFGLVKDIAR FRPDLKVLIS SATIDAEKFS AYFDEAPVFY VPGRRYPVDI 

       610        620        630        640        650        660 
YYTPQPEANY IQAAITTILQ IHTTQPAGDI LVFLTGQDEI ELMSENMQEL CRILGKRIPE 

       670        680        690        700        710        720 
IILCPIYANL PSELQAKIFD PTPPGARKVV LATNIAETSI TIDGVNFVID SGFVKQNMYN 

       730        740        750        760        770        780 
PRTGMESLVS VPCSRASADQ RAGRAGRVGP GKCFRLYTRR TYNNELDMVT SPEIQRTNLT 

       790        800        810        820        830        840 
NIVLLLKSLG INNLLDFDFM DAPPPETLMR SLELLYALGA LNNRGELTKL GRQMAEFPTD 

       850        860        870        880        890        900 
PMLSKSLIAS SKYGCVEEVL SIVSMLGEAS SLFYRPKDKI MEADKARANF TQPGGDHLTL 

       910        920        930        940        950        960 
LHIWNEWVDT DFSYNWAREN FLQYKSLCRA RDVRDQLANL CERVEIELVT NSSESLDPIK 

       970        980        990       1000       1010       1020 
KAITAGYFSN AARLDRSGDS YRTVKSNQTV YIHPSSSVAE KKPKVIIYFE LVLTTKEYCR 

      1030       1040       1050 
QITEIQPEWL LEISPHYFKP ENIEELQKTQ KRHKR 

« Hide

References

« Hide 'large scale' references
[1]"A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase."
Lundgren K., Allan S., Urushiyama S., Tani T., Ohshima Y., Frendewey D., Beach D.
Mol. Biol. Cell 7:1083-1094(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe."
Urushiyama S., Tani T., Ohshima Y.
Mol. Gen. Genet. 253:118-127(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48733 Genomic DNA. Translation: AAC49377.1. Frameshift.
CU329671 Genomic DNA. Translation: CAB57929.2.
PIRT46568.
T50372.
RefSeqNP_595686.2. NM_001021581.3.

3D structure databases

ProteinModelPortalQ10752.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277230. 11 interactions.
STRING4896.SPBC19C2.01-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC19C2.01.1; SPBC19C2.01.1:pep; SPBC19C2.01.
GeneID2540707.
KEGGspo:SPBC19C2.01.

Organism-specific databases

PomBaseSPBC19C2.01.

Phylogenomic databases

eggNOGCOG1643.
HOGENOMHOG000175261.
KOK12813.
OMAIEIESKW.
OrthoDBEOG7M98QW.
PhylomeDBQ10752.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801829.
PROQ10752.

Entry information

Entry nameCDC28_SCHPO
AccessionPrimary (citable) accession number: Q10752
Secondary accession number(s): Q9URU1, Q9USV9, Q9UUD7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 29, 2001
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names