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Protein

C-X-C motif chemokine 3

Gene

Cxcl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.By similarity2 Publications

GO - Molecular functioni

  • chemokine activity Source: UniProtKB

GO - Biological processi

  • cellular response to lipopolysaccharide Source: RGD
  • immune response Source: InterPro
  • inflammatory response Source: RGD
  • neutrophil chemotaxis Source: UniProtKB
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • response to lipopolysaccharide Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiR-RNO-380108. Chemokine receptors bind chemokines.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-X-C motif chemokine 3
Alternative name(s):
Cytokine-induced neutrophil chemoattractant 2
Short name:
CINC-2
Macrophage inflammatory protein 2-alpha/beta
Short name:
MIP2-alpha/beta
Gene namesi
Name:Cxcl3
Synonyms:Cinc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi621812. Cxcl3.

Subcellular locationi

GO - Cellular componenti

  • cell Source: GOC
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 32322 PublicationsAdd
BLAST
Chaini33 – 10169C-X-C motif chemokine 3PRO_0000144297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 63By similarity
Disulfide bondi39 ↔ 79By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ10746.

PTM databases

PhosphoSiteiQ10746.

Expressioni

Inductioni

By lipopolysaccharide. Expression increases until 4 hours after treatment and then gradually decreases, remaining high 24 hours after stimulation.1 Publication

Gene expression databases

GenevisibleiQ10746. RN.

Interactioni

GO - Molecular functioni

  • chemokine activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038520.

Structurei

3D structure databases

ProteinModelPortaliQ10746.
SMRiQ10746. Positions 30-100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J3WE. Eukaryota.
ENOG410ZT1C. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiQ10746.
KOiK05505.
OMAiEVCLNPQ.
OrthoDBiEOG7GTT6P.
PhylomeDBiQ10746.
TreeFamiTF333433.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q10746-1) [UniParc]FASTAAdd to basket

Also known as: CINC-2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPTRRLLN AALLLLLLLM ATSHQPSGTV VARELRCQCL KTLPRVDFEN
60 70 80 90 100
IQSLTVTPPG PHCTQTEVIA TLKDGQEVCL NPQAPRLQKI IQKLLKSDKS

S
Length:101
Mass (Da):11,109
Last modified:November 25, 2008 - v2
Checksum:iD949D5712FE30909
GO
Isoform 2 (identifier: Q10746-2) [UniParc]FASTAAdd to basket

Also known as: CINC-2B

The sequence of this isoform differs from the canonical sequence as follows:
     98-101: DKSS → PSL

Show »
Length:100
Mass (Da):10,989
Checksum:iEB53ECEFE30909D2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 1014DKSS → PSL in isoform 2. 1 PublicationVSP_035735

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21095 mRNA. Translation: BAA04657.1.
D87926 Genomic DNA. Translation: BAB12336.1.
D87926 Genomic DNA. Translation: BAB12279.1.
D87927 mRNA. Translation: BAB12280.1.
PIRiS46198.
RefSeqiNP_612531.1. NM_138522.1. [Q10746-2]
XP_006250783.1. XM_006250721.2. [Q10746-1]
UniGeneiRn.10525.

Genome annotation databases

EnsembliENSRNOT00000034090; ENSRNOP00000038520; ENSRNOG00000028043. [Q10746-2]
ENSRNOT00000078746; ENSRNOP00000070985; ENSRNOG00000028043. [Q10746-1]
GeneIDi171551.
KEGGirno:171551.
UCSCiRGD:621812. rat. [Q10746-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21095 mRNA. Translation: BAA04657.1.
D87926 Genomic DNA. Translation: BAB12336.1.
D87926 Genomic DNA. Translation: BAB12279.1.
D87927 mRNA. Translation: BAB12280.1.
PIRiS46198.
RefSeqiNP_612531.1. NM_138522.1. [Q10746-2]
XP_006250783.1. XM_006250721.2. [Q10746-1]
UniGeneiRn.10525.

3D structure databases

ProteinModelPortaliQ10746.
SMRiQ10746. Positions 30-100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038520.

PTM databases

PhosphoSiteiQ10746.

Proteomic databases

PaxDbiQ10746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000034090; ENSRNOP00000038520; ENSRNOG00000028043. [Q10746-2]
ENSRNOT00000078746; ENSRNOP00000070985; ENSRNOG00000028043. [Q10746-1]
GeneIDi171551.
KEGGirno:171551.
UCSCiRGD:621812. rat. [Q10746-1]

Organism-specific databases

CTDi2921.
RGDi621812. Cxcl3.

Phylogenomic databases

eggNOGiENOG410J3WE. Eukaryota.
ENOG410ZT1C. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiQ10746.
KOiK05505.
OMAiEVCLNPQ.
OrthoDBiEOG7GTT6P.
PhylomeDBiQ10746.
TreeFamiTF333433.

Enzyme and pathway databases

ReactomeiR-RNO-380108. Chemokine receptors bind chemokines.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

NextBioi622533.
PROiQ10746.

Gene expression databases

GenevisibleiQ10746. RN.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of cytokine-induced neutrophil chemoattractants (CINC), rat GRO/CINC-2 alpha and CINC-2 beta, produced by granulation tissue in culture: purification, complete amino acid sequences and characterization."
    Nakagawa H., Komorita N., Shibata F., Ikesue A., Konishi K., Fujioka M., Kato H.
    Biochem. J. 301:545-550(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 33-100 (ISOFORM 1), PROTEIN SEQUENCE OF 33-67 (ISOFORM 2), FUNCTION.
    Strain: Wistar.
    Tissue: Peritoneal cavity.
  2. "Gene structure, cDNA cloning, and expression of the rat cytokine-induced neutrophil chemoattractant-2 (CINC-2) gene."
    Shibata F., Konishi K., Nakagawa H.
    Cytokine 10:169-174(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2), INDUCTION.
    Strain: Wistar.
    Tissue: Peritoneal cavity.
  3. "Production of an interleukin-8-like chemokine by cytokine-stimulated rat NRK-49F fibroblasts and its suppression by anti-inflammatory steroids."
    Nakagawa H., Ikesue A., Hatakeyama S., Kato H., Gotoda T., Komorita N., Watanabe K., Miyai H.
    Biochem. Pharmacol. 45:1425-1430(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-56.
    Tissue: Fibroblast.
  4. "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
    Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
    Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-61, FUNCTION.
    Strain: Wistar.

Entry informationi

Entry nameiCXCL3_RAT
AccessioniPrimary (citable) accession number: Q10746
Secondary accession number(s): Q10747, Q5CZ55, Q9EP62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 25, 2008
Last modified: May 11, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.