Reviewed,
UniProtKB/Swiss-Prot Q10739 (MMP14_RAT)
Last modified
October 13, 2009.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Matrix metalloproteinase-14 Short name=MMP-14 EC=3.4.24.80 Alternative name(s): Membrane-type matrix metalloproteinase 1 Short name=MT-MMP 1 Short name=MTMMP1 Membrane-type-1 matrix metalloproteinase Short name=MT1-MMP Short name=MT1MMP Short name=MT-MMP | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 582 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. |
| Catalytic activity | Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. Calcium By similarity. |
| Subcellular location | Membrane; Single-pass type I membrane protein Potential. Melanosome By similarity. |
| Domain | The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. |
| Sequence similarities | Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 20 | 20 | Potential | ||||||||
| Propeptide | 21 – 111 | 91 | Activation peptide | PRO_0000028806 | |||||||
| Chain | 112 – 582 | 471 | Matrix metalloproteinase-14 | PRO_0000028807 | |||||||
Regions | |||||||||||
| Topological domain | 112 – 541 | 430 | Extracellular Potential | ||||||||
| Transmembrane | 542 – 562 | 21 | Potential | ||||||||
| Topological domain | 563 – 582 | 20 | Cytoplasmic Potential | ||||||||
| Domain | 323 – 366 | 44 | Hemopexin-like 1 | ||||||||
| Domain | 368 – 412 | 45 | Hemopexin-like 2 | ||||||||
| Domain | 415 – 461 | 47 | Hemopexin-like 3 | ||||||||
| Domain | 463 – 508 | 46 | Hemopexin-like 4 | ||||||||
| Motif | 91 – 98 | 8 | Cysteine switch By similarity | ||||||||
Sites | |||||||||||
| Active site | 240 | 1 | By similarity | ||||||||
| Metal binding | 93 | 1 | Zinc; in inhibited form By similarity | ||||||||
| Metal binding | 239 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 243 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 249 | 1 | Zinc; catalytic By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 319 ↔ 508 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 68 | 1 | M → I in CAA58521. Ref.1 | ||||||||
| Sequence conflict | 255 | 1 | A → D in CAA58521. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas." Okada A., Bellocq J.-P., Rouyer N., Chenard M.P., Rio M.C., Chambon P., Basset P. Proc. Natl. Acad. Sci. U.S.A. 92:2730-2734(1995) [PubMed: 7708715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Expression and function of MT-MMP in glial cells." Cossins J., Clements J., Catlin G., Wells G. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X83537 mRNA. Translation: CAA58521.1. X91785 mRNA. Translation: CAA62897.1. BC072509 mRNA. Translation: AAH72509.1. | |
| IPI | IPI00327562. |
| PIR | I84471. |
| RefSeq | NP_112318.1. |
| UniGene | Rn.10371 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BQQ based on UniProtKB P50281. |
| SMR | Q10739. Positions 114-287. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q10739. |
Protein family/group databases | |
| MEROPS | M10.014. |
PTM databases | |
| PhosphoSite | Q10739. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000015101; ENSRNOP00000015101; ENSRNOG00000010947; Rattus norvegicus. [Genome view] ENSRNOT00000042938; ENSRNOP00000049168; ENSRNOG00000010947; Rattus norvegicus. [Genome view] |
| GeneID | 81707. |
| KEGG | rno:81707. |
| UCSC | NM_031056. rat. |
Organism-specific databases | |
| CTD | 81707. |
| RGD | 620198. Mmp14. |
Phylogenomic databases | |
| HOVERGEN | Q10739. |
Enzyme and pathway databases | |
| BRENDA | 3.4.24.80. 248. |
Gene expression databases | |
| ArrayExpress | Q10739. |
| Genevestigator | Q10739. |
| GermOnline | ENSRNOG00000010947. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR016293. Pept_M10A_matrix. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view] |
| Gene3D | G3DSA:2.110.10.10. Hemopexin. 1 hit. |
| Pfam | PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF001191. Peptidase_M10A_matrix. 1 hit. |
| PRINTS | PR00138. MATRIXIN. |
| SMART | SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view] |
| PROSITE | PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 615328. |
Entry information
| Entry name | MMP14_RAT | ||||||||
| Accession | Primary (citable) accession number: Q10739 Secondary accession number(s): Q6IN06 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


