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Q10739

- MMP14_RAT

UniProt

Q10739 - MMP14_RAT

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Protein
Matrix metalloproteinase-14
Gene
Mmp14, Mtmmp
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7 By similarity. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues By similarity.

Catalytic activityi

Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.

Cofactori

Binds 1 zinc ion per subunit By similarity.
Calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi93 – 931Zinc; in inhibited form By similarity
Metal bindingi239 – 2391Zinc; catalytic By similarity
Active sitei240 – 2401 By similarity
Metal bindingi243 – 2431Zinc; catalytic By similarity
Metal bindingi249 – 2491Zinc; catalytic By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. integrin binding Source: RGD
  3. metalloendopeptidase activity Source: InterPro
  4. peptidase activator activity Source: RGD
  5. sequence-specific DNA binding transcription factor activity Source: Ensembl
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. angiogenesis Source: RGD
  2. astrocyte cell migration Source: RGD
  3. branching morphogenesis of an epithelial tube Source: Ensembl
  4. endothelial cell proliferation Source: RGD
  5. lung development Source: Ensembl
  6. male gonad development Source: RGD
  7. negative regulation of focal adhesion assembly Source: RGD
  8. ossification Source: RGD
  9. ovarian follicle development Source: RGD
  10. positive regulation of cell growth Source: Ensembl
  11. positive regulation of cell migration Source: Ensembl
  12. positive regulation of peptidase activity Source: GOC
  13. response to estrogen Source: RGD
  14. response to hormone Source: RGD
  15. response to hypoxia Source: RGD
  16. response to mechanical stimulus Source: RGD
  17. response to organic cyclic compound Source: RGD
  18. response to oxidative stress Source: RGD
  19. tissue remodeling Source: RGD
  20. zymogen activation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198590. Activation of Matrix Metalloproteinases.
REACT_199177. Collagen degradation.
REACT_199178. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiM10.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-14 (EC:3.4.24.80)
Short name:
MMP-14
Alternative name(s):
Membrane-type matrix metalloproteinase 1
Short name:
MT-MMP 1
Short name:
MTMMP1
Membrane-type-1 matrix metalloproteinase
Short name:
MT-MMP
Short name:
MT1-MMP
Short name:
MT1MMP
Gene namesi
Name:Mmp14
Synonyms:Mtmmp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi620198. Mmp14.

Subcellular locationi

Membrane; Single-pass type I membrane protein Reviewed prediction. Melanosome By similarity. Cytoplasm By similarity
Note: Forms a complex with BST2 and localizes to the cytoplasm By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini112 – 541430Extracellular Reviewed prediction
Add
BLAST
Transmembranei542 – 56221Helical; Reviewed prediction
Add
BLAST
Topological domaini563 – 58220Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. extracellular matrix Source: InterPro
  3. integral component of membrane Source: UniProtKB-KW
  4. melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Propeptidei21 – 11191Activation peptide
PRO_0000028806Add
BLAST
Chaini112 – 582471Matrix metalloproteinase-14
PRO_0000028807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi319 ↔ 508 By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

PRIDEiQ10739.

PTM databases

PhosphoSiteiQ10739.

Expressioni

Gene expression databases

GenevestigatoriQ10739.

Interactioni

Subunit structurei

Interacts (via C-terminal cytoplasmic tail) with BST2 By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049168.

Structurei

3D structure databases

ProteinModelPortaliQ10739.
SMRiQ10739. Positions 114-287.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati316 – 36449Hemopexin 1
Add
BLAST
Repeati365 – 41046Hemopexin 2
Add
BLAST
Repeati412 – 46049Hemopexin 3
Add
BLAST
Repeati461 – 50848Hemopexin 4
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi91 – 988Cysteine switch By similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.
Contains 4 hemopexin repeats.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295915.
GeneTreeiENSGT00750000117332.
HOGENOMiHOG000217928.
HOVERGENiHBG052484.
InParanoidiQ10739.
KOiK07763.
OMAiWMGCPSG.
OrthoDBiEOG7XPZ57.
PhylomeDBiQ10739.
TreeFamiTF352396.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028693. MMP14.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF24. PTHR10201:SF24. 1 hit.
PfamiPF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10739-1 [UniParc]FASTAAdd to Basket

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MSPAPRPSRS LLLPLLTLGT TLASLGWAQS SNFSPEAWLQ QYGYLPPGDL    50
RTHTQRSPQS LSAAIAAMQR FYGLQVTGKA DSDTMKAMRR PRCGVPDKFG 100
TEIKANVRRK RYAIQGLKWQ HNEITFCIQN YTPKVGEYAT FEAIRKAFRV 150
WESATPLRFR EVPYAYIREG HEKQADIMIL FAEGFHGDST PFDGEGGFLA 200
HAYFPGPNIG GDTHFDSAEP WTVQNEDLNG NDIFLVAVHE LGHALGLEHS 250
NDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYGSKSGSP TKMPPQPRTT 300
SRPSVPDKPR NPTYGPNICD GNFDTVAMLR GEMFVFKERW FWRVRNNQVM 350
DGYPMPIGQF WRGLPASINT AYERKDGKFV FFKGDKHWVF DEASLEPGYP 400
KHIKELGRGL PTDKIDAALF WMPNGKTYFF RGNKYYRFNE EFRAVDSEYP 450
KNIKVWEGIP ESPRGSFMGS DEVFTYFYKG NKYWKFNNQK LKVEPGYPKS 500
ALRDWMGCPS GGRPDEGTEE ETEVIIIEVD EEGSGAVSAA AVVLPVLLLL 550
LVLAVGLAVF FFRRHGTPKR LLYCQRSLLD KV 582
Length:582
Mass (Da):66,080
Last modified:May 1, 2007 - v2
Checksum:i48F1A8E3065E1AE8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681M → I in CAA58521. 1 Publication
Sequence conflicti255 – 2551A → D in CAA58521. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83537 mRNA. Translation: CAA58521.1.
X91785 mRNA. Translation: CAA62897.1.
BC072509 mRNA. Translation: AAH72509.1.
PIRiI84471.
RefSeqiNP_112318.1. NM_031056.1.
UniGeneiRn.10371.

Genome annotation databases

EnsembliENSRNOT00000042938; ENSRNOP00000049168; ENSRNOG00000010947.
ENSRNOT00000075610; ENSRNOP00000065434; ENSRNOG00000010947.
GeneIDi81707.
KEGGirno:81707.
UCSCiRGD:620198. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83537 mRNA. Translation: CAA58521.1 .
X91785 mRNA. Translation: CAA62897.1 .
BC072509 mRNA. Translation: AAH72509.1 .
PIRi I84471.
RefSeqi NP_112318.1. NM_031056.1.
UniGenei Rn.10371.

3D structure databases

ProteinModelPortali Q10739.
SMRi Q10739. Positions 114-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000049168.

Protein family/group databases

MEROPSi M10.014.

PTM databases

PhosphoSitei Q10739.

Proteomic databases

PRIDEi Q10739.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000042938 ; ENSRNOP00000049168 ; ENSRNOG00000010947 .
ENSRNOT00000075610 ; ENSRNOP00000065434 ; ENSRNOG00000010947 .
GeneIDi 81707.
KEGGi rno:81707.
UCSCi RGD:620198. rat.

Organism-specific databases

CTDi 4323.
RGDi 620198. Mmp14.

Phylogenomic databases

eggNOGi NOG295915.
GeneTreei ENSGT00750000117332.
HOGENOMi HOG000217928.
HOVERGENi HBG052484.
InParanoidi Q10739.
KOi K07763.
OMAi WMGCPSG.
OrthoDBi EOG7XPZ57.
PhylomeDBi Q10739.
TreeFami TF352396.

Enzyme and pathway databases

Reactomei REACT_198590. Activation of Matrix Metalloproteinases.
REACT_199177. Collagen degradation.
REACT_199178. Degradation of the extracellular matrix.

Miscellaneous databases

NextBioi 615328.
PROi Q10739.

Gene expression databases

Genevestigatori Q10739.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028693. MMP14.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
PANTHERi PTHR10201:SF24. PTHR10201:SF24. 1 hit.
Pfami PF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas."
    Okada A., Bellocq J.-P., Rouyer N., Chenard M.P., Rio M.C., Chambon P., Basset P.
    Proc. Natl. Acad. Sci. U.S.A. 92:2730-2734(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression and function of MT-MMP in glial cells."
    Cossins J., Clements J., Catlin G., Wells G.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiMMP14_RAT
AccessioniPrimary (citable) accession number: Q10739
Secondary accession number(s): Q6IN06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 1, 2007
Last modified: September 3, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi