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Protein

Aminopeptidase N

Gene
N/A
Organism
Haemonchus contortus (Barber pole worm)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208SubstrateBy similarity1
Metal bindingi379Zinc; catalyticPROSITE-ProRule annotation1
Active sitei380Proton acceptorPROSITE-ProRule annotation1
Metal bindingi383Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi402Zinc; catalyticPROSITE-ProRule annotation1
Sitei466Transition state stabilizerBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Short name:
AP-N
Alternative name(s):
Membrane glycoprotein H11
Microsomal aminopeptidase
OrganismiHaemonchus contortus (Barber pole worm)
Taxonomic identifieri6289 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 17CytoplasmicSequence analysisAdd BLAST16
Transmembranei18 – 39Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST22
Topological domaini40 – 972ExtracellularSequence analysisAdd BLAST933

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000950872 – 972Aminopeptidase NAdd BLAST971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi549N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi759 ↔ 766By similarity
Disulfide bondi804 ↔ 840By similarity
Glycosylationi858N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ10737.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 347Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

CDDicd09601. M1_APN_2. 1 hit.
InterProiView protein in InterPro
IPR024571. ERAP1-like_C_dom.
IPR034016. M1_APN-typ.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiView protein in Pfam
PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
PRINTSiPR00756. ALADIPTASE.
PROSITEiView protein in PROSITE
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q10737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSQGRTRTL LNLTPIRLIV ALFLVAAAVG LSIGLTYYFT RKAFDTSEKP
60 70 80 90 100
GKDDTGGKDK DNSPSAAELL LPSNIKPLSY DLTIKTYLPG YVDFPPEKNL
110 120 130 140 150
TFDGRVEISM VVIEPTKSIV LNSKKISVIP QECELVSGDK KLEIESVKEH
160 170 180 190 200
PRLEKVEFLI KSQLEKDQQI LLKVGYIGLI SNSFGGIYQT TYTTPDGTPK
210 220 230 240 250
IAAVSQNEPI DARRMVPCMD EPKYKANWTV TVIHPKGTKA VSNGIEVNGD
260 270 280 290 300
GEISGDWITS KFLTTPRMSS YLLAVMVSEF EYIEGETKTG VRFRIWSRPE
310 320 330 340 350
AKKMTQYALQ SGIKCIEFYE DFFDIRFPLK KQDMIALPDF SAGAMENWGL
360 370 380 390 400
ITYRENSLLY DDRFYAPMNK QRIARIVAHE LAHQWFGDLV TMKWWDNLWL
410 420 430 440 450
NEGFARFTEF IGAGQITQDD ARMRNYFLID VLERALKADS VASSHPLSFR
460 470 480 490 500
IDKAAEVEEA FDDITYAKGA SVLTMLRALI GEEKHKHAVS QYLKKFSYSN
510 520 530 540 550
AEATDLWAVF DEVVTDVEGP DGKPMKTTEF ASQWTTQMGF PVISVAEFNS
560 570 580 590 600
TTLKLTQSRY EANKDAVEKE KYRHPKYGFK WDIPLWYQEG DKKEIKRTWL
610 620 630 640 650
RRDEPLYLHV SDAGAPFVVN ADRYGFYRQN HDANGWKKII KQLKDNHEVY
660 670 680 690 700
SPRTRNAIIS DAFAAAATDA IEYETVFELL NYAEKETEYL PLEIAMSGIS
710 720 730 740 750
SILKYFGTEP EAKPAQTYMM NILKPMYEKS SIDFIANNYR NDKLFFQINL
760 770 780 790 800
QKDVIDMFCA LGSQDCRKKY KKLFDDEVMN KCRDGQAATE CVRIAAPLRS
810 820 830 840 850
SVYCYGVKEG GDYASDKVME LYTAETLALE KDFLRLALGC HKDVTALKGL
860 870 880 890 900
LLRALDRNSS FVRMQDIPSA FNDVAANPIG GEFIFNFLIE RWPDIIESIG
910 920 930 940 950
TKHTYVEKVI PACTSGIRSQ QQIDQLKNLQ KNGMNARQFG AFDKAIERAQ
960 970
NRVDWIKKHF QKLAAFFKKA TL
Length:972
Mass (Da):110,674
Last modified:January 23, 2007 - v2
Checksum:i5964328CC3C80E43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94187 mRNA. Translation: CAA63897.1.

Similar proteinsi

Entry informationi

Entry nameiAMPN_HAECO
AccessioniPrimary (citable) accession number: Q10737
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families