Reviewed, UniProtKB/Swiss-Prot Q10737 (AMPN_HAECO)

Names and origin

Protein names	Recommended name:     Aminopeptidase N       Short name=AP-N     EC=3.4.11.2 Alternative name(s):     Microsomal aminopeptidase     Membrane glycoprotein H11
Organism	Haemonchus contortus (Barber pole worm)
Taxonomic identifier	6289 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Strongylida › Trichostrongyloidea › Haemonchidae › Haemonchinae › Haemonchus

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 972	971	Aminopeptidase N		PRO_0000095087
Regions
	Topological domain	2 – 17	16	Cytoplasmic Potential
	Transmembrane	18 – 39	22	Signal-anchor for type II membrane protein Potential
	Topological domain	40 – 972	933	Extracellular Potential
Sites
	Active site	380	1	By similarity
	Active site	466	1	Proton donor Potential
	Metal binding	379	1	Zinc; catalytic By similarity
	Metal binding	383	1	Zinc; catalytic By similarity
	Metal binding	402	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	227	1	N-linked (GlcNAc...) Potential
	Glycosylation	549	1	N-linked (GlcNAc...) Potential
	Glycosylation	858	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10737-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5964328CC3C80E43	FASTA	972	110,674
	10 20 30 40 50 60 MTSQGRTRTL LNLTPIRLIV ALFLVAAAVG LSIGLTYYFT RKAFDTSEKP GKDDTGGKDK 70 80 90 100 110 120 DNSPSAAELL LPSNIKPLSY DLTIKTYLPG YVDFPPEKNL TFDGRVEISM VVIEPTKSIV 130 140 150 160 170 180 LNSKKISVIP QECELVSGDK KLEIESVKEH PRLEKVEFLI KSQLEKDQQI LLKVGYIGLI 190 200 210 220 230 240 SNSFGGIYQT TYTTPDGTPK IAAVSQNEPI DARRMVPCMD EPKYKANWTV TVIHPKGTKA 250 260 270 280 290 300 VSNGIEVNGD GEISGDWITS KFLTTPRMSS YLLAVMVSEF EYIEGETKTG VRFRIWSRPE 310 320 330 340 350 360 AKKMTQYALQ SGIKCIEFYE DFFDIRFPLK KQDMIALPDF SAGAMENWGL ITYRENSLLY 370 380 390 400 410 420 DDRFYAPMNK QRIARIVAHE LAHQWFGDLV TMKWWDNLWL NEGFARFTEF IGAGQITQDD 430 440 450 460 470 480 ARMRNYFLID VLERALKADS VASSHPLSFR IDKAAEVEEA FDDITYAKGA SVLTMLRALI 490 500 510 520 530 540 GEEKHKHAVS QYLKKFSYSN AEATDLWAVF DEVVTDVEGP DGKPMKTTEF ASQWTTQMGF 550 560 570 580 590 600 PVISVAEFNS TTLKLTQSRY EANKDAVEKE KYRHPKYGFK WDIPLWYQEG DKKEIKRTWL 610 620 630 640 650 660 RRDEPLYLHV SDAGAPFVVN ADRYGFYRQN HDANGWKKII KQLKDNHEVY SPRTRNAIIS 670 680 690 700 710 720 DAFAAAATDA IEYETVFELL NYAEKETEYL PLEIAMSGIS SILKYFGTEP EAKPAQTYMM 730 740 750 760 770 780 NILKPMYEKS SIDFIANNYR NDKLFFQINL QKDVIDMFCA LGSQDCRKKY KKLFDDEVMN 790 800 810 820 830 840 KCRDGQAATE CVRIAAPLRS SVYCYGVKEG GDYASDKVME LYTAETLALE KDFLRLALGC 850 860 870 880 890 900 HKDVTALKGL LLRALDRNSS FVRMQDIPSA FNDVAANPIG GEFIFNFLIE RWPDIIESIG 910 920 930 940 950 960 TKHTYVEKVI PACTSGIRSQ QQIDQLKNLQ KNGMNARQFG AFDKAIERAQ NRVDWIKKHF 970 QKLAAFFKKA TL

References

[1]

"Cloning and characterization of a microsomal aminopeptidase from the intestine of the nematode Haemonchus contortus." Smith T.S., Graham M., Munn E.A., Newton S.E., Knox D.P., Coadwell W.J., McMichael-Phillips D., Smith H., Smith W.D., Oliver J.J. Biochim. Biophys. Acta 1338:295-306(1997) [PubMed: 9128148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94187 mRNA. Translation: CAA63897.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M01.015.
Enzyme and pathway databases
BRENDA	3.4.11.2. 2482.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_HAECO

Accession

Primary (citable) accession number: Q10737

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)