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Q10730

- AMPN_LACHE

UniProt

Q10730 - AMPN_LACHE

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Protein

Aminopeptidase N

Gene

pepN

Organism
Lactobacillus helveticus (Lactobacillus suntoryeus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201SubstrateBy similarity
Metal bindingi289 – 2891Zinc; catalyticPROSITE-ProRule annotation
Active sitei290 – 2901Proton acceptorPROSITE-ProRule annotation
Metal bindingi293 – 2931Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi312 – 3121Zinc; catalyticPROSITE-ProRule annotation
Sitei376 – 3761Transition state stabilizerBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
OrganismiLactobacillus helveticus (Lactobacillus suntoryeus)
Taxonomic identifieri1587 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 844844Aminopeptidase NPRO_0000095072Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ10730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni253 – 2575Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10730-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVKRFYKTF HPEHYDLRIN VNRKNKTING TSTITGDVFE NPVLINQKFM
60 70 80 90 100
TIDSVKVDGK NVDFDVIEKD EAIKIKTGVT GKAVIEIAYS APLTDTMMGI
110 120 130 140 150
YPSYYELEGK KKQIIGTQFE TTFARQAFPC VDEPEAKATF SLALKWDEQD
160 170 180 190 200
GEVALANMPE VEVDKDGYHH FEETVRMSSY LVAFAFGELQ SKTTHTKDGV
210 220 230 240 250
LIGVYATKAH KPKELDFALD IAKRAIEFYE EFYQTKYPLP QSLQLALPDF
260 270 280 290 300
SAGAMENWGL VTYREAYLLL DPDNTSLEMK KLVATVITHE LAHQWFGDLV
310 320 330 340 350
TMKWWDNLWL NESFANMMEY LSVDGLEPDW HIWEMFQTSE ASSALNRDAT
360 370 380 390 400
DGVQPIQMEI NDPADIDSVF DSAIVYAKGS RMLVMVRSLL GDDALRKGLK
410 420 430 440 450
YYFDHHKFGN ATGDDLWDAL STATDLDIGK IMHSWLKQPG YPVVNAFVAE
460 470 480 490 500
DGHLKLTQKQ FFIGEGEDKG RQWQIPLNAN FDAPKIMSDK EIDLGSYKVL
510 520 530 540 550
REEAGHPLRL NVGNNSHFIV EYDKTLHDDI LSDVNELDPI DKLQLLQDLR
560 570 580 590 600
LLAEGKQISY ASIVPLLVKF ADSKSSLVIN ALYTTAAKLR QFVEPESNEE
610 620 630 640 650
KNLKKLYDLL SKDQVARLGW EVKPGESDED VQIRPYELSA SLYAENADSI
660 670 680 690 700
KAAHQIFTEN EDNLEALNAD IRPYVLINEV KNFGNAELVD KLIKEYQRTA
710 720 730 740 750
DPSYKVDLRS AVTSTKDLAA IKAIVGDFEN ADVVKPQDLC DWYRGLLANH
760 770 780 790 800
YGQQAAWDWI REDWDWLDKT VGGDMEFAKF ITVTAGVFHT PERLKEFKEF
810 820 830 840
FEPKINVPLL SREIKMDVKV IESKVNLIEA EKDAVNDAVA KAID
Length:844
Mass (Da):95,838
Last modified:October 1, 1996 - v1
Checksum:i1EA5DE6AC69410F4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti39 – 391F → I in strain: 53/7.
Natural varianti44 – 441L → F in strain: 53/7.
Natural varianti342 – 3421S → A in strain: 53/7.
Natural varianti455 – 4551K → R in strain: 53/7.
Natural varianti496 – 4961S → N in strain: 53/7.
Natural varianti527 – 5271H → L in strain: 53/7.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08224 Genomic DNA. Translation: AAA81951.1.
Z30323 Genomic DNA. Translation: CAA82978.1.
PIRiJC4054.
S47274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08224 Genomic DNA. Translation: AAA81951.1 .
Z30323 Genomic DNA. Translation: CAA82978.1 .
PIRi JC4054.
S47274.

3D structure databases

ProteinModelPortali Q10730.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M01.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis, distribution and expression of an aminopeptidase N-encoding gene from Lactobacillus helveticus CNRZ32."
    Christensen J.E., Lin D.-L., Palva A., Steele J.L.
    Gene 155:89-93(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CNRZ 32.
  2. "Characterization and expression of the pepN gene encoding a general aminopeptidase from Lactobacillus helveticus."
    Varmanen P., Vesanto E., Steele J.L., Palva A.
    FEMS Microbiol. Lett. 124:315-320(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 53/7.

Entry informationi

Entry nameiAMPN_LACHE
AccessioniPrimary (citable) accession number: Q10730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3