Reviewed, UniProtKB/Swiss-Prot Q10726 (KAX61_PANIM)

Names and origin

Protein names	Recommended name:     Potassium channel toxin alpha-KTx 6.1 Alternative name(s):     Potassium channel-blocking toxin 1       Short name=Pi-1       Short name=Pi1     PiTX-K-gamma
Organism	Pandinus imperator (Emperor scorpion)
Taxonomic identifier	55084 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Scorpionoidea › Scorpionidae › Scorpioninae › Pandinus

Protein attributes

Sequence length	35 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Potently and reversibly blocks voltage-gated shaker B (Sh) potassium channels.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 6 subfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 35

35

Potassium channel toxin alpha-KTx 6.1

PRO_0000044912

Sites

Site

24

1

Critical for activity By similarity

Amino acid modifications

Disulfide bond

4 ↔ 25

Ref.4 Ref.5 Ref.1

Disulfide bond

10 ↔ 30

Ref.4 Ref.5 Ref.1

Disulfide bond

14 ↔ 32

Ref.4 Ref.5 Ref.1

Disulfide bond

20 ↔ 35

Ref.4 Ref.5 Ref.1

Experimental info

Sequence conflict

20

1

C → A Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

35

Helix Strand Turn

Details...

Turn

6 – 10

5

Helix

11 – 17

7

Beta strand

24 – 26

3

Beta strand

29 – 31

3

Sequences

	Sequence		Length	Mass (Da)
	Q10726-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 208001C82B2C9800	FASTA	35	3,843
	10 20 30 LVKCRGTSDC GRPCQQQTGC PNSKCINRMC KCYGC

References

[1]	"A novel structural class of K+-channel blocking toxin from the scorpion Pandinus imperator." Olamendi-Portugal T., Gomez-Lagunas F., Gurrola G.B., Possani L.D. Biochem. J. 315:977-981(1996) [PubMed: 8645186] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Three new toxins from the scorpion Pandinus imperator selectively block certain voltage-gated K+ channels." Rogowski R.S., Collins J.H., O'Neill T.J., Gustafson T.A., Werkman T.R., Rogawski M.A., Tenenholz T.C., Weber D.J., Blaustein M.P. Mol. Pharmacol. 50:1167-1177(1996) [PubMed: 8913348] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[3]	"Two novel toxins from the venom of the scorpion Pandinus imperator show that the N-terminal amino acid sequence is important for their affinities towards Shaker B K+ channels." Gomez-Lagunas F., Olamendi-Portugal T., Zamudio F.Z., Possani L.D. J. Membr. Biol. 152:49-56(1996) [PubMed: 8660410] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[4]	"A novel potassium channel blocking toxin from the scorpion Pandinus imperator: a 1H NMR analysis using a nano-NMR probe." Delepierre M., Prochnicka-Chalufour A., Possani L.D. Biochemistry 36:2649-2658(1997) [PubMed: 9054572] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.
[5]	"The impact of the fourth disulfide bridge in scorpion toxins of the alpha-KTx6 subfamily." Carrega L., Mosbah A., Ferrat G., Beeton C., Andreotti N., Mansuelle P., Darbon H., De Waard M., Sabatier J.M. Proteins 61:1010-1023(2005) [PubMed: 16247791] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

S69599.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WZ5	NMR	-	A	1-34	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR001947. Scorpion_toxinS.
[Graphical view]

ProDom

PD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KAX61_PANIM

Accession

Primary (citable) accession number: Q10726

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)