Reviewed, UniProtKB/Swiss-Prot Q10680 (COBK_MYCTU)

Names and origin

Protein names

Recommended name:
    Precorrin-6A reductase
    EC=1.3.1.54
Alternative name(s):
    Precorrin-6X reductase

Gene names

Name:	cobK
Ordered Locus Names:	Rv2070c, MT2130
ORF Names:	MTCY49.09c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the macrocycle of precorrin-6X into precorrin-6Y By similarity.
Catalytic activity	Precorrin-6B + NADP(+) = precorrin-6A + NADPH.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinic acid a,c-diamide from precorrin-2 (aerobic route): step 6/10.
Sequence similarities	Belongs to the precorrin-6x reductase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	precorrin-6A reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Precorrin-6A reductase		PRO_0000135919

Sequences

	Sequence		Length	Mass (Da)
	Q10680-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 946A3F5E7740807C	FASTA	244	25,737
	10 20 30 40 50 60 MTRVLLLGGT AEGRALAKEL HPHVEIVSSL AGRVPNPALP IGPVRIGGFG GVEGLRGWLR 70 80 90 100 110 120 EERIDAVVDA THPFAVTITA HAAQVCGELG LPYLVLARPP WDPGTAIIAV SDIEAADVVA 130 140 150 160 170 180 EQGYSRVFLT TGRSGIAAFA NSDAWFLIRV VTAPDGTALP RRHKLVLSRG PYGYHDEFAL 190 200 210 220 230 240 LREQRIDALV TKNSGGKMTR AKLDAAAALG ISVVMIARPL LPAGVAAVDS VHRAAMWVAG LPSR

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842578 Genomic DNA. Translation: CAA98217.1. AE000516 Genomic DNA. Translation: AAK46410.1.
PIR	A70765.
RefSeq	NP_216586.1. NP_336596.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	887566. 924600.
GenomeReviews	Gene locus MT2130 in contig AE000516_GR. Gene locus Rv2070c in contig AL123456_GR.
KEGG	mtc:MT2130. mtu:Rv2070c.
TIGR	MT2130.
Organism-specific databases
TubercuList	Rv2070c.
Phylogenomic databases
HOGENOM	Q10680.
Family and domain databases
InterPro	IPR003723. Cbl_synth_CobK/CbiJ. [Graphical view]
Pfam	PF02571. CbiJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00715. precor6x_red. 1 hit.
PROSITE	PS51014. COBK_CBIJ. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q10680.

Entry information

Entry name

COBK_MYCTU

Accession

Primary (citable) accession number: Q10680

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)