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Protein

Dual specificity mitogen-activated protein kinase kinase mek-2

Gene

mek-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the sur-1/mpk-1 gene product in controlling vulval cell differentiation (PubMed:7729690). Required for progression of developing oocytes through the pachytene stage (PubMed:19826475). Plays a role in responses to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation (PubMed:15268855). Involved in fluid homeostasis (PubMed:11689700). Positively regulates lifespan upstream of mpk-1 (PubMed:20624915).5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation catalyzed by MAP kinase kinase kinases.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021ATPPROSITE-ProRule annotation
Active sitei195 – 1951Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 879ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • meiotic cell cycle Source: UniProtKB-KW
  • oogenesis Source: UniProtKB-KW
  • protein phosphorylation Source: WormBase
  • Ras protein signal transduction Source: WormBase
  • vulval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Differentiation, Meiosis, Oogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 1045.
ReactomeiR-CEL-110056. MAPK3 (ERK1) activation.
R-CEL-112411. MAPK1 (ERK2) activation.
R-CEL-445144. Signal transduction by L1.
R-CEL-5674135. MAP2K and MAPK activation.
R-CEL-5674499. Negative feedback regulation of MAPK pathway.
SignaLinkiQ10664.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase mek-2 (EC:2.7.12.2)
Short name:
MAP kinase kinase mek-2
Gene namesi
Name:mek-2
ORF Names:Y54E10BL.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiY54E10BL.6; CE25437; WBGene00003186; mek-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes a defect in pachytene progression resulting in a proximal gonad devoid of nuclei. The phenotype is more severe in gck-1 km15 mutant background (PubMed:19826475). RNAi-mediated knockdown in adults decreases lifespan (PubMed:20624915).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi213 – 2131D → N in n2678; rescues fluid accumulation in crl-1 e1745ts mutant. 1 Publication
Mutagenesisi217 – 2171S → F in n1989; severe constipation following M.nematophilium infection. 1 Publication
Mutagenesisi223 – 2231S → E: Phosphomimetic mutant which, in a wild type background, induces tail swelling; in association with D-227.
Mutagenesisi227 – 2271S → D: Phosphomimetic mutant which, in a wild type background, induces tail swelling; in association with E-223. 1 Publication
Mutagenesisi237 – 2371P → S in KU114; 8% larval lethality. 1 Publication
Mutagenesisi238 – 2381E → K in H294; 100% larval lethality. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Dual specificity mitogen-activated protein kinase kinase mek-2PRO_0000086322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei223 – 2231PhosphoserineBy similarity
Modified residuei227 – 2271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ10664.
PaxDbiQ10664.
PeptideAtlasiQ10664.
PRIDEiQ10664.

PTM databases

iPTMnetiQ10664.

Interactioni

Subunit structurei

Interacts with ksr-1.1 Publication

Protein-protein interaction databases

BioGridi37349. 5 interactions.
IntActiQ10664. 3 interactions.
MINTiMINT-114232.
STRINGi6239.Y54E10BL.6.

Structurei

3D structure databases

ProteinModelPortaliQ10664.
SMRiQ10664. Positions 44-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 360288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
InParanoidiQ10664.
KOiK04368.
OMAiHHERHII.
OrthoDBiEOG7HF1KZ.
PhylomeDBiQ10664.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGKRRNPL GLSLPPTVNE QSESGEATAE EATATVPLEE QLKKLGLTEP
60 70 80 90 100
QTQRLSEFLQ VKEGIKELSE DMLQTEGELG HGNGGVVNKC VHRKTGVIMA
110 120 130 140 150
RKLVHLEIKP SVRQQIVKEL AVLHKCNSPF IVGFYGAFVD NNDISICMEY
160 170 180 190 200
MDGLSLDIVL KKVGRLPEKF VGRISVAVVR GLTYLKDEIK ILHRDVKPSN
210 220 230 240 250
MLVNSNGEIK LCDFGVSGML IDSMANSFVG TRSYMAPERL TGSHYTISSD
260 270 280 290 300
IWSFGLSLVE LLIGRYPVPA PSQAEYATMF NVAENEIELA DSLEEPNYHP
310 320 330 340 350
PSNPASMAIF EMLDYIVNGP PPTLPKRFFT DEVIGFVSKC LRKLPSERAT
360 370 380
LKSLTADVFF TQYADHDDQG EFAVFVKGTI NLPKLNP
Length:387
Mass (Da):42,794
Last modified:November 1, 1996 - v1
Checksum:i8FD8556236B6624B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21107 mRNA. Translation: AAA85118.1.
FO081804 Genomic DNA. Translation: CCD73487.1.
PIRiA56466.
RefSeqiNP_491087.1. NM_058686.4.
UniGeneiCel.19739.

Genome annotation databases

EnsemblMetazoaiY54E10BL.6; Y54E10BL.6; WBGene00003186.
GeneIDi171872.
KEGGicel:CELE_Y54E10BL.6.
UCSCiY54E10BL.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21107 mRNA. Translation: AAA85118.1.
FO081804 Genomic DNA. Translation: CCD73487.1.
PIRiA56466.
RefSeqiNP_491087.1. NM_058686.4.
UniGeneiCel.19739.

3D structure databases

ProteinModelPortaliQ10664.
SMRiQ10664. Positions 44-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37349. 5 interactions.
IntActiQ10664. 3 interactions.
MINTiMINT-114232.
STRINGi6239.Y54E10BL.6.

PTM databases

iPTMnetiQ10664.

Proteomic databases

EPDiQ10664.
PaxDbiQ10664.
PeptideAtlasiQ10664.
PRIDEiQ10664.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY54E10BL.6; Y54E10BL.6; WBGene00003186.
GeneIDi171872.
KEGGicel:CELE_Y54E10BL.6.
UCSCiY54E10BL.6. c. elegans.

Organism-specific databases

CTDi171872.
WormBaseiY54E10BL.6; CE25437; WBGene00003186; mek-2.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
InParanoidiQ10664.
KOiK04368.
OMAiHHERHII.
OrthoDBiEOG7HF1KZ.
PhylomeDBiQ10664.

Enzyme and pathway databases

BRENDAi2.7.12.2. 1045.
ReactomeiR-CEL-110056. MAPK3 (ERK1) activation.
R-CEL-112411. MAPK1 (ERK2) activation.
R-CEL-445144. Signal transduction by L1.
R-CEL-5674135. MAP2K and MAPK activation.
R-CEL-5674499. Negative feedback regulation of MAPK pathway.
SignaLinkiQ10664.

Miscellaneous databases

PROiQ10664.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events."
    Wu Y., Han M., Guan K.-L.
    Genes Dev. 9:742-755(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-237 AND GLU-238.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization."
    Stewart S., Sundaram M., Zhang Y., Lee J., Han M., Guan K.L.
    Mol. Cell. Biol. 19:5523-5534(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KSR-1.
  4. "The Caenorhabditis elegans EGL-15 signaling pathway implicates a DOS-like multisubstrate adaptor protein in fibroblast growth factor signal transduction."
    Schutzman J.L., Borland C.Z., Newman J.C., Robinson M.K., Kokel M., Stern M.J.
    Mol. Cell. Biol. 21:8104-8116(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-213.
    Strain: Bristol N2.
  5. "The ERK MAP kinase cascade mediates tail swelling and a protective response to rectal infection in C. elegans."
    Nicholas H.R., Hodgkin J.
    Curr. Biol. 14:1256-1261(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-217; SER-223 AND SER-227.
  6. "The germinal center kinase GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C. elegans germline."
    Schouest K.R., Kurasawa Y., Furuta T., Hisamoto N., Matsumoto K., Schumacher J.M.
    PLoS ONE 4:E7450-E7450(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "The ERK-MAPK pathway regulates longevity through SKN-1 and insulin-like signaling in Caenorhabditis elegans."
    Okuyama T., Inoue H., Ookuma S., Satoh T., Kano K., Honjoh S., Hisamoto N., Matsumoto K., Nishida E.
    J. Biol. Chem. 285:30274-30281(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMEK2_CAEEL
AccessioniPrimary (citable) accession number: Q10664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.