Dual specificity mitogen-activated protein kinase kinase mek-2

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Reviewed, UniProtKB/Swiss-Prot Q10664 (MEK2_CAEEL)

Last modified February 9, 2010. Version 75. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase mek-2
      Short name=MAP kinase kinase mek-2
    EC=2.7.12.2

Gene names

Name:	mek-2
ORF Names:	Y54E10BL.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Functions in the let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the sur-1/mpK-1 gene product in controlling vulval cell differentiation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation catalyzed by MAP kinase kinase kinases.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase gastrulation with mouth forming first Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 387

387

Dual specificity mitogen-activated protein kinase kinase mek-2

PRO_0000086322

Regions

Domain

73 – 360

288

Protein kinase

Nucleotide binding

79 – 87

ATP By similarity

Sites

Active site

195

Proton acceptor By similarity

Binding site

102

ATP By similarity

Amino acid modifications

Modified residue

223

Phosphoserine By similarity

Modified residue

227

Phosphoserine By similarity

Experimental info

Mutagenesis

237

P → S in KU114; 8% larval lethality. Ref.1

Mutagenesis

238

E → K in H294; 100% larval lethality. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q10664-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8FD8556236B6624B
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FASTA

387

42,794

        10         20         30         40         50         60 
MSSGKRRNPL GLSLPPTVNE QSESGEATAE EATATVPLEE QLKKLGLTEP QTQRLSEFLQ 

        70         80         90        100        110        120 
VKEGIKELSE DMLQTEGELG HGNGGVVNKC VHRKTGVIMA RKLVHLEIKP SVRQQIVKEL 

       130        140        150        160        170        180 
AVLHKCNSPF IVGFYGAFVD NNDISICMEY MDGLSLDIVL KKVGRLPEKF VGRISVAVVR 

       190        200        210        220        230        240 
GLTYLKDEIK ILHRDVKPSN MLVNSNGEIK LCDFGVSGML IDSMANSFVG TRSYMAPERL 

       250        260        270        280        290        300 
TGSHYTISSD IWSFGLSLVE LLIGRYPVPA PSQAEYATMF NVAENEIELA DSLEEPNYHP 

       310        320        330        340        350        360 
PSNPASMAIF EMLDYIVNGP PPTLPKRFFT DEVIGFVSKC LRKLPSERAT LKSLTADVFF 

       370        380 
TQYADHDDQG EFAVFVKGTI NLPKLNP

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events." Wu Y., Han M., Guan K.-L. Genes Dev. 9:742-755(1995) [PubMed: 7729690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-237 AND GLU-238.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	U21107 mRNA. Translation: AAA85118.1. AC024811 Genomic DNA. Translation: AAF60779.1.
PIR	A56466.
RefSeq	NP_491087.1.
UniGene	Cel.19739
3D structure databases
SMR	Q10664. Positions 62-382.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10664. 3 interactions.
STRING	Q10664.
Genome annotation databases
Ensembl	Y54E10BL.6; Y54E10BL.6; Y54E10BL.6; Caenorhabditis elegans. [Genome view]
GeneID	171872.
KEGG	cel:Y54E10BL.6.
NMPDR	fig\|6239.3.peg.549.
UCSC	Y54E10BL.6. c. elegans.
Organism-specific databases
CTD	171872.
WormBase	WBGene00003186. mek-2.
WormPep	Y54E10BL.6. CE25437. [WorfDB]
Phylogenomic databases
eggNOG	meNOG05601.
HOGENOM	HBG755340.
InParanoid	Q10664.
OMA	GWVCKTM.
PhylomeDB	Q10664.
Enzyme and pathway databases
BRENDA	2.7.12.2. 672.
Gene expression databases
ArrayExpress	Q10664.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	873047.

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Entry information

Entry name

MEK2_CAEEL

Accession

Primary (citable) accession number: Q10664

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents