Reviewed, UniProtKB/Swiss-Prot Q10664 (MEK2_CAEEL)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase mek-2
      Short name=MAP kinase kinase mek-2
    EC=2.7.12.2

Gene names

Name:	mek-2
ORF Names:	Y54E10BL.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions in the let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the sur-1/mpK-1 gene product in controlling vulval cell differentiation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation catalyzed by MAP kinase kinase kinases.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase gastrulation with mouth forming first Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Dual specificity mitogen-activated protein kinase kinase mek-2		PRO_0000086322
Regions
	Domain	73 – 360	288	Protein kinase
	Nucleotide binding	79 – 87	9	ATP By similarity
Sites
	Active site	195	1	Proton acceptor By similarity
	Binding site	102	1	ATP By similarity
Amino acid modifications
	Modified residue	223	1	Phosphoserine By similarity
	Modified residue	227	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	237	1	P → S in KU114; 8% larval lethality. Ref.1
	Mutagenesis	238	1	E → K in H294; 100% larval lethality. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q10664-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8FD8556236B6624B	FASTA	387	42,794
	10 20 30 40 50 60 MSSGKRRNPL GLSLPPTVNE QSESGEATAE EATATVPLEE QLKKLGLTEP QTQRLSEFLQ 70 80 90 100 110 120 VKEGIKELSE DMLQTEGELG HGNGGVVNKC VHRKTGVIMA RKLVHLEIKP SVRQQIVKEL 130 140 150 160 170 180 AVLHKCNSPF IVGFYGAFVD NNDISICMEY MDGLSLDIVL KKVGRLPEKF VGRISVAVVR 190 200 210 220 230 240 GLTYLKDEIK ILHRDVKPSN MLVNSNGEIK LCDFGVSGML IDSMANSFVG TRSYMAPERL 250 260 270 280 290 300 TGSHYTISSD IWSFGLSLVE LLIGRYPVPA PSQAEYATMF NVAENEIELA DSLEEPNYHP 310 320 330 340 350 360 PSNPASMAIF EMLDYIVNGP PPTLPKRFFT DEVIGFVSKC LRKLPSERAT LKSLTADVFF 370 380 TQYADHDDQG EFAVFVKGTI NLPKLNP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events." Wu Y., Han M., Guan K.-L. Genes Dev. 9:742-755(1995) [PubMed: 7729690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-237 AND GLU-238.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U21107 mRNA. Translation: AAA85118.1. AC024811 Genomic DNA. Translation: AAF60779.1.
PIR	A56466.
RefSeq	NP_491087.1.
UniGene	Cel.19739
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10664. 3 interactions.
STRING	Q10664.
Genome annotation databases
Ensembl	Y54E10BL.6; Y54E10BL.6; Y54E10BL.6; Caenorhabditis elegans. [Genome view]
GeneID	171872.
KEGG	cel:Y54E10BL.6.
NMPDR	fig|6239.3.peg.549.
UCSC	Y54E10BL.6. c. elegans.
Organism-specific databases
CTD	171872.
WormBase	WBGene00003186. mek-2.
WormPep	Y54E10BL.6. CE25437. [WorfDB]
Phylogenomic databases
OMA	SHYTISS.
Enzyme and pathway databases
BRENDA	2.7.12.2. 672.
Gene expression databases
ArrayExpress	Q10664.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	873047.

Entry information

Entry name

MEK2_CAEEL

Accession

Primary (citable) accession number: Q10664

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project