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Q10664 (MEK2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dual specificity mitogen-activated protein kinase kinase mek-2

Short name=MAP kinase kinase mek-2
EC=2.7.12.2
Gene names
Name:mek-2
ORF Names:Y54E10BL.6
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in the let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the sur-1/mpK-1 gene product in controlling vulval cell differentiation.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation catalyzed by MAP kinase kinase kinases.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Dual specificity mitogen-activated protein kinase kinase mek-2
PRO_0000086322

Regions

Domain73 – 360288Protein kinase
Nucleotide binding79 – 879ATP By similarity

Sites

Active site1951Proton acceptor By similarity
Binding site1021ATP By similarity

Amino acid modifications

Modified residue2231Phosphoserine By similarity
Modified residue2271Phosphoserine By similarity

Experimental info

Mutagenesis2371P → S in KU114; 8% larval lethality. Ref.1
Mutagenesis2381E → K in H294; 100% larval lethality. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q10664 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8FD8556236B6624B

FASTA38742,794
        10         20         30         40         50         60 
MSSGKRRNPL GLSLPPTVNE QSESGEATAE EATATVPLEE QLKKLGLTEP QTQRLSEFLQ 

        70         80         90        100        110        120 
VKEGIKELSE DMLQTEGELG HGNGGVVNKC VHRKTGVIMA RKLVHLEIKP SVRQQIVKEL 

       130        140        150        160        170        180 
AVLHKCNSPF IVGFYGAFVD NNDISICMEY MDGLSLDIVL KKVGRLPEKF VGRISVAVVR 

       190        200        210        220        230        240 
GLTYLKDEIK ILHRDVKPSN MLVNSNGEIK LCDFGVSGML IDSMANSFVG TRSYMAPERL 

       250        260        270        280        290        300 
TGSHYTISSD IWSFGLSLVE LLIGRYPVPA PSQAEYATMF NVAENEIELA DSLEEPNYHP 

       310        320        330        340        350        360 
PSNPASMAIF EMLDYIVNGP PPTLPKRFFT DEVIGFVSKC LRKLPSERAT LKSLTADVFF 

       370        380 
TQYADHDDQG EFAVFVKGTI NLPKLNP 

« Hide

References

« Hide 'large scale' references
[1]"MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events."
Wu Y., Han M., Guan K.-L.
Genes Dev. 9:742-755(1995) [PubMed: 7729690] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-237 AND GLU-238.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U21107 mRNA. Translation: AAA85118.1.
FO081804 Genomic DNA. Translation: CCD73487.1.
PIRA56466.
RefSeqNP_491087.1. NM_058686.3.
UniGeneCel.19739.

3D structure databases

ProteinModelPortalQ10664.
SMRQ10664. Positions 48-382.
ModBaseSearch...

Protein-protein interaction databases

IntActQ10664. 3 interactions.
MINTMINT-114232.
STRINGQ10664.

Proteomic databases

PRIDEQ10664.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaY54E10BL.6; Y54E10BL.6; Y54E10BL.6.
GeneID171872.
KEGGcel:Y54E10BL.6.
NMPDRfig|6239.3.peg.549.
UCSCY54E10BL.6. c. elegans.

Organism-specific databases

CTD171872.
WormBaseY54E10BL.6; CE25437; WBGene00003186; mek-2.

Phylogenomic databases

eggNOGmeNOG05601.
GeneTreeEMGT00050000000989.
HOGENOMHBG755340.
InParanoidQ10664.
OMADIAGWVC.
PhylomeDBQ10664.

Enzyme and pathway databases

BRENDA2.7.12.2. 1045.

Gene expression databases

ArrayExpressQ10664.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR002290. Ser/Thr_kinase_dom.
[Graphical view]
KOK04368.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio873047.

Entry information

Entry nameMEK2_CAEEL
AccessionPrimary (citable) accession number: Q10664
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: November 1, 1996
Last modified: January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families