Q10663 (GCP_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional glyoxylate cycle protein

Alternative name(s):
Gex-3-interacting protein 7

Including the following 2 domains:

1. Isocitrate lyase
   Short name=ICL
   Short name=Isocitrase
   Short name=Isocitratase
   EC=4.1.3.1
2. Malate synthase
   EC=2.3.3.9

Gene names

Name:	gei-7
ORF Names:	C05E4.9

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate. Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2. Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Tissue specificity	Intestinal and body wall muscle cells.
Developmental stage	First detected early in the generation of the clonal E cell lineage that is committed to intestinal development and then increases to maximal levels in actively differentiating intestinal and body wall muscle cells. Highest activity in embryos and falls dramatically during L1 larval development.
Sequence similarities	In the N-terminal section; belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family. In the C-terminal section; belongs to the malate synthase family.
Sequence caution	The sequence AAA85857.1 differs from that shown. Reason: Chimeric cDNA.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Molecular function	Lyase Transferase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	determination of adult lifespan Inferred from genetic interaction PubMed 12845331. Source: WormBase embryo development Inferred from physical interaction PubMed 11922622. Source: WormBase glyoxylate cycle Inferred from sequence or structural similarity PubMed 11922622. Source: WormBase tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from direct assay PubMed 20188671. Source: WormBase
Molecular_function	isocitrate lyase activity Inferred from sequence or structural similarity PubMed 11922622. Source: WormBase malate synthase activity Inferred from sequence or structural similarity PubMed 11922622. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 968	968	Bifunctional glyoxylate cycle protein		PRO_0000166874
Regions
	Region	1 – 443	443	Isocitrate lyase
	Region	444 – 968	525	Malate synthase
Sites
	Active site	601	1	Proton acceptor By similarity
	Active site	881	1	Proton donor By similarity
Experimental info
	Sequence conflict	152	1	D → H in AAA85857. Ref.1
	Sequence conflict	436	1	A → R in AAA85857. Ref.1
	Sequence conflict	463	1	R → A in AAA85857. Ref.1
	Sequence conflict	957 – 968	12	TDAYD…VSEGY → DRRLRQACL in AAA85857. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q10663 [UniParc]. Last modified June 20, 2003. Version 2. Checksum: 7BAAF979C1F6D31F	FASTA	968	108,629
	10 20 30 40 50 60 MSSAAKNFYQ VVKSAPKGRF KGIKRDYTVE DVLKLRGSID IDYTLATRGA NKLWQLLHTE 70 80 90 100 110 120 PFVPALGAQT GNQAVQMVRA GLKAIYLSGW QVAADANSAG DMYPDQSLYP ANSGPELAKR 130 140 150 160 170 180 INRSLRRADQ IEACEAEDYL AQRDWYAPIV ADAEAGFGGA LNCFELMKAY IEAGAAGVHY 190 200 210 220 230 240 EDQLGSEKKC GHMGGKVLIP TAQHIRHLNA SRLAADVCGV PTIIVARTDA ESSRLLTSDI 250 260 270 280 290 300 DPRDHPYIDY EAGRTIEGFY RLKDSTAIQY CIDRAIQYAP YTDLIWMETS HPTIADAREF 310 320 330 340 350 360 AEGVHKQYPD KMFAYNCSPS FNWKKHLSPS QMEKFQKELG AMGFKYQFIT LAGYHANSYS 370 380 390 400 410 420 MFDLARNYKE KGMLAYSGLQ EGEFAAEKHG YTAVKHQREV GTGYFDAVSR AVTGGLSSTT 430 440 450 460 470 480 ALSGSTEEAQ FQTAVASQDE EILSLTAQNV AGDEKILTPD ALRFLHDLNT EFNPRRLRLL 490 500 510 520 530 540 SKRNQVQADI NNSLWFPDFN KETEVLRSDQ GWKGAEIPRD LQDRRVEITG PTDRKMVINA 550 560 570 580 590 600 MNSGANVFMA DFEDSNSPTW RNQLEGQINL YDAVRNNISY THPTTKKEYT LNEKHAVLKV 610 620 630 640 650 660 RPRGWHLPEK HVLIHNQPTS GSLFDFGLFV FHNAKALIAQ GSGPYFYLPK LQSAEEAQLW 670 680 690 700 710 720 ADVFKYTEDK LGLARGTIKC TVLIEHLLAS FQLHEIIHAL KDNIVGLNCG RWDYIFSYIK 730 740 750 760 770 780 TFQNHRKFLL PDRFQIGMTA PFMRNYSLEV IKACHLRGIH AMGGMAAQIP IKHDQVANDK 790 800 810 820 830 840 AFALVRADKE REATDGHDGT WVAHPGLVPL AKRVFDQMMP KPNQISKNLT RANCTKEDLT 850 860 870 880 890 900 VIPEGTRTEA GFRHNISVTL GYLDSWLRGT GCVPLYNLME DAATAEISRA QLWQWLHHDA 910 920 930 940 950 960 KLEDGRTIDA GLVKQTIAAE TERRLIRAGS VVNRIPEAAD LLEKFVTEEK MSDFLTTDAY DRLVSEGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle." Liu F., Thatcher J.D., Barral J.M., Epstein H.F. Dev. Biol. 169:399-414(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 429-461. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23159 mRNA. Translation: AAA85857.1. Sequence problems. FO080368 Genomic DNA. Translation: CCD63238.1.
PIR	E88940.
RefSeq	NP_503306.1. NM_070905.5.
3D structure databases
ProteinModelPortal	Q10663.
SMR	Q10663. Positions 7-432, 455-963.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25113N.
MINT	MINT-1060277.
STRING	6239.C05E4.9a.
Proteomic databases
PaxDb	Q10663.
PRIDE	Q10663.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C05E4.9a; C05E4.9a; C05E4.9.
GeneID	178583.
KEGG	cel:CELE_C05E4.9.
UCSC	C05E4.9a. c. elegans.
Organism-specific databases
CTD	178583.
WormBase	C05E4.9a; CE23521; WBGene00001564; gei-7.
Phylogenomic databases
eggNOG	COG2225.
GeneTree	ENSGT00390000002932.
HOGENOM	HOG000112704.
InParanoid	Q10663.
OMA	IWMETSH.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719. UPA00703; UER00720.
Gene expression databases
ArrayExpress	Q10663.
Family and domain databases
Gene3D	3.20.20.60. 1 hit.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR011076. Malate_synth-like. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00463. ICL. 1 hit. PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit. TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	901736.

Entry information

Entry name

GCP_CAEEL

Accession

Primary (citable) accession number: Q10663
Secondary accession number(s): O17353

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 20, 2003
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project