Reviewed, UniProtKB/Swiss-Prot Q10663 (GCP_CAEEL)

Names and origin

Protein names

Recommended name:
    Bifunctional glyoxylate cycle protein
Alternative name(s):
    Gex-3-interacting protein 7
Including the following 2 domains:
    1- Recommended name:
            Isocitrate lyase
                Short name=Isocitratase
                Short name=Isocitrase
                Short name=ICL
              EC=4.1.3.1
    2- Recommended name:
            Malate synthase
              EC=2.3.3.9

Gene names

Name:	gei-7
ORF Names:	C05E4.9

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate. Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2. Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Tissue specificity	Intestinal and body wall muscle cells.
Developmental stage	First detected early in the generation of the clonal E cell lineage that is committed to intestinal development and then increases to maximal levels in actively differentiating intestinal and body wall muscle cells. Highest activity in embryos and falls dramatically during L1 larval development.
Sequence similarities	In the N-terminal section; belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family. In the C-terminal section; belongs to the malate synthase family.
Sequence caution	The sequence AAA85857.1 differs from that shown. Reason: Miscellaneous discrepancy. Chimeric cDNA.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Molecular function	Lyase Transferase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	determination of adult lifespan Inferred from mutant phenotype. Source: WormBase glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate lyase activity Inferred from electronic annotation. Source: EC malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 968	968	Bifunctional glyoxylate cycle protein		PRO_0000166874
Regions
	Region	1 – 443	443	Isocitrate lyase
	Region	444 – 968	525	Malate synthase
Sites
	Active site	601	1	Proton acceptor By similarity
	Active site	881	1	Proton donor By similarity
Experimental info
	Sequence conflict	152	1	D → H in AAA85857. Ref.1
	Sequence conflict	436	1	A → R in AAA85857. Ref.1
	Sequence conflict	463	1	R → A in AAA85857. Ref.1
	Sequence conflict	957 – 968	12	TDAYD…VSEGY → DRRLRQACL in AAA85857. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q10663-1 [UniParc]. Last modified June 20, 2003. Version 2. Checksum: 7BAAF979C1F6D31F	FASTA	968	108,629
	10 20 30 40 50 60 MSSAAKNFYQ VVKSAPKGRF KGIKRDYTVE DVLKLRGSID IDYTLATRGA NKLWQLLHTE 70 80 90 100 110 120 PFVPALGAQT GNQAVQMVRA GLKAIYLSGW QVAADANSAG DMYPDQSLYP ANSGPELAKR 130 140 150 160 170 180 INRSLRRADQ IEACEAEDYL AQRDWYAPIV ADAEAGFGGA LNCFELMKAY IEAGAAGVHY 190 200 210 220 230 240 EDQLGSEKKC GHMGGKVLIP TAQHIRHLNA SRLAADVCGV PTIIVARTDA ESSRLLTSDI 250 260 270 280 290 300 DPRDHPYIDY EAGRTIEGFY RLKDSTAIQY CIDRAIQYAP YTDLIWMETS HPTIADAREF 310 320 330 340 350 360 AEGVHKQYPD KMFAYNCSPS FNWKKHLSPS QMEKFQKELG AMGFKYQFIT LAGYHANSYS 370 380 390 400 410 420 MFDLARNYKE KGMLAYSGLQ EGEFAAEKHG YTAVKHQREV GTGYFDAVSR AVTGGLSSTT 430 440 450 460 470 480 ALSGSTEEAQ FQTAVASQDE EILSLTAQNV AGDEKILTPD ALRFLHDLNT EFNPRRLRLL 490 500 510 520 530 540 SKRNQVQADI NNSLWFPDFN KETEVLRSDQ GWKGAEIPRD LQDRRVEITG PTDRKMVINA 550 560 570 580 590 600 MNSGANVFMA DFEDSNSPTW RNQLEGQINL YDAVRNNISY THPTTKKEYT LNEKHAVLKV 610 620 630 640 650 660 RPRGWHLPEK HVLIHNQPTS GSLFDFGLFV FHNAKALIAQ GSGPYFYLPK LQSAEEAQLW 670 680 690 700 710 720 ADVFKYTEDK LGLARGTIKC TVLIEHLLAS FQLHEIIHAL KDNIVGLNCG RWDYIFSYIK 730 740 750 760 770 780 TFQNHRKFLL PDRFQIGMTA PFMRNYSLEV IKACHLRGIH AMGGMAAQIP IKHDQVANDK 790 800 810 820 830 840 AFALVRADKE REATDGHDGT WVAHPGLVPL AKRVFDQMMP KPNQISKNLT RANCTKEDLT 850 860 870 880 890 900 VIPEGTRTEA GFRHNISVTL GYLDSWLRGT GCVPLYNLME DAATAEISRA QLWQWLHHDA 910 920 930 940 950 960 KLEDGRTIDA GLVKQTIAAE TERRLIRAGS VVNRIPEAAD LLEKFVTEEK MSDFLTTDAY DRLVSEGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle." Liu F., Thatcher J.D., Barral J.M., Epstein H.F. Dev. Biol. 169:399-414(1995) [PubMed: 7781887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 429-461. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23159 mRNA. Translation: AAA85857.1. Sequence problems. AF026209 Genomic DNA. Translation: AAB71278.2.
PIR	E88940.
RefSeq	NP_503306.1.
UniGene	Cel.18040
3D structure databases
SMR	Q10663. Positions 19-431, 530-904.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10663. 1 interaction.
STRING	Q10663.
Proteomic databases
PRIDE	Q10663.
Genome annotation databases
Ensembl	C05E4.9a; C05E4.9a; C05E4.9; Caenorhabditis elegans. [Genome view]
GeneID	178583.
UCSC	C05E4.9a. c. elegans.
Organism-specific databases
CTD	178583.
WormBase	WBGene00001564. gei-7.
WormPep	C05E4.9a. CE23521. [WorfDB]
Phylogenomic databases
eggNOG	meNOG07067.
InParanoid	Q10663.
OMA	WRNQLEG.
PhylomeDB	Q10663.
Enzyme and pathway databases
BRENDA	2.3.3.9. 672. 4.1.3.1. 672.
Gene expression databases
ArrayExpress	Q10663.
Family and domain databases
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR019830. Malate_synthase_CS. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR21631:SF3. Isocit_lyase. 1 hit.
Pfam	PF00463. ICL. 1 hit. PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit. TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	901736.

Entry information

Entry name

GCP_CAEEL

Accession

Primary (citable) accession number: Q10663
Secondary accession number(s): O17353

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 20, 2003
Last modified:	February 9, 2010
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project