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Q10663

- GCP_CAEEL

UniProt

Q10663 - GCP_CAEEL

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Protein

Bifunctional glyoxylate cycle protein

Gene

icl-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation
Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei601 – 6011Proton acceptorPROSITE-ProRule annotation
Active sitei881 – 8811Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. isocitrate lyase activity Source: WormBase
  2. malate synthase activity Source: WormBase

GO - Biological processi

  1. determination of adult lifespan Source: WormBase
  2. embryo development Source: WormBase
  3. glyoxylate cycle Source: WormBase
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.
UPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glyoxylate cycle protein
Alternative name(s):
Gex-3-interacting protein 7
Including the following 2 domains:
Isocitrate lyase (EC:4.1.3.1)
Short name:
ICL
Short name:
Isocitrase
Short name:
Isocitratase
Malate synthase (EC:2.3.3.9)
Gene namesi
Name:icl-1
Synonyms:gei-7
ORF Names:C05E4.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiC05E4.9a; CE23521; WBGene00001564; icl-1.
C05E4.9b; CE32565; WBGene00001564; icl-1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Bifunctional glyoxylate cycle proteinPRO_0000166874Add
BLAST

Proteomic databases

PaxDbiQ10663.
PRIDEiQ10663.

Expressioni

Tissue specificityi

Intestinal and body wall muscle cells.

Developmental stagei

First detected early in the generation of the clonal E cell lineage that is committed to intestinal development and then increases to maximal levels in actively differentiating intestinal and body wall muscle cells. Highest activity in embryos and falls dramatically during L1 larval development (PubMed:7781887). Expression is highest in L3 larval stage with low levels at other stages (PubMed:21884719).2 Publications

Gene expression databases

ExpressionAtlasiQ10663. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi43657. 1 interaction.
DIPiDIP-25113N.
MINTiMINT-1060277.
STRINGi6239.C05E4.9a.

Structurei

3D structure databases

ProteinModelPortaliQ10663.
SMRiQ10663. Positions 7-432, 455-963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 443443Isocitrate lyaseAdd
BLAST
Regioni444 – 968525Malate synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.Curated
In the C-terminal section; belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
GeneTreeiENSGT00390000002932.
HOGENOMiHOG000112704.
InParanoidiQ10663.
OMAiIWMETSH.
OrthoDBiEOG771267.
PhylomeDBiQ10663.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
PF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
SSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
TIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q10663-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAAKNFYQ VVKSAPKGRF KGIKRDYTVE DVLKLRGSID IDYTLATRGA
60 70 80 90 100
NKLWQLLHTE PFVPALGAQT GNQAVQMVRA GLKAIYLSGW QVAADANSAG
110 120 130 140 150
DMYPDQSLYP ANSGPELAKR INRSLRRADQ IEACEAEDYL AQRDWYAPIV
160 170 180 190 200
ADAEAGFGGA LNCFELMKAY IEAGAAGVHY EDQLGSEKKC GHMGGKVLIP
210 220 230 240 250
TAQHIRHLNA SRLAADVCGV PTIIVARTDA ESSRLLTSDI DPRDHPYIDY
260 270 280 290 300
EAGRTIEGFY RLKDSTAIQY CIDRAIQYAP YTDLIWMETS HPTIADAREF
310 320 330 340 350
AEGVHKQYPD KMFAYNCSPS FNWKKHLSPS QMEKFQKELG AMGFKYQFIT
360 370 380 390 400
LAGYHANSYS MFDLARNYKE KGMLAYSGLQ EGEFAAEKHG YTAVKHQREV
410 420 430 440 450
GTGYFDAVSR AVTGGLSSTT ALSGSTEEAQ FQTAVASQDE EILSLTAQNV
460 470 480 490 500
AGDEKILTPD ALRFLHDLNT EFNPRRLRLL SKRNQVQADI NNSLWFPDFN
510 520 530 540 550
KETEVLRSDQ GWKGAEIPRD LQDRRVEITG PTDRKMVINA MNSGANVFMA
560 570 580 590 600
DFEDSNSPTW RNQLEGQINL YDAVRNNISY THPTTKKEYT LNEKHAVLKV
610 620 630 640 650
RPRGWHLPEK HVLIHNQPTS GSLFDFGLFV FHNAKALIAQ GSGPYFYLPK
660 670 680 690 700
LQSAEEAQLW ADVFKYTEDK LGLARGTIKC TVLIEHLLAS FQLHEIIHAL
710 720 730 740 750
KDNIVGLNCG RWDYIFSYIK TFQNHRKFLL PDRFQIGMTA PFMRNYSLEV
760 770 780 790 800
IKACHLRGIH AMGGMAAQIP IKHDQVANDK AFALVRADKE REATDGHDGT
810 820 830 840 850
WVAHPGLVPL AKRVFDQMMP KPNQISKNLT RANCTKEDLT VIPEGTRTEA
860 870 880 890 900
GFRHNISVTL GYLDSWLRGT GCVPLYNLME DAATAEISRA QLWQWLHHDA
910 920 930 940 950
KLEDGRTIDA GLVKQTIAAE TERRLIRAGS VVNRIPEAAD LLEKFVTEEK
960
MSDFLTTDAY DRLVSEGY
Length:968
Mass (Da):108,629
Last modified:June 20, 2003 - v2
Checksum:i7BAAF979C1F6D31F
GO
Isoform b (identifier: Q10663-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     862-863: YL → EP
     864-968: Missing.

Note: No experimental confirmation available.

Show »
Length:863
Mass (Da):96,743
Checksum:i5392FF3F102D0903
GO

Sequence cautioni

The sequence AAA85857.1 differs from that shown. Reason: Chimeric cDNA.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521D → H in AAA85857. (PubMed:7781887)Curated
Sequence conflicti436 – 4361A → R in AAA85857. (PubMed:7781887)Curated
Sequence conflicti463 – 4631R → A in AAA85857. (PubMed:7781887)Curated
Sequence conflicti957 – 96812TDAYD…VSEGY → DRRLRQACL in AAA85857. (PubMed:7781887)CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei862 – 8632YL → EP in isoform b. CuratedVSP_056035
Alternative sequencei864 – 968105Missing in isoform b. CuratedVSP_056036Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23159 mRNA. Translation: AAA85857.1. Sequence problems.
FO080368 Genomic DNA. Translation: CCD63238.1.
FO080368 Genomic DNA. Translation: CCD63239.1.
PIRiE88940.
RefSeqiNP_001021367.1. NM_001026196.4.
NP_503306.1. NM_070905.5.
UniGeneiCel.18040.

Genome annotation databases

EnsemblMetazoaiC05E4.9a; C05E4.9a; WBGene00001564. [Q10663-1]
C05E4.9b; C05E4.9b; WBGene00001564. [Q10663-2]
GeneIDi178583.
KEGGicel:CELE_C05E4.9.
UCSCiC05E4.9a. c. elegans. [Q10663-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23159 mRNA. Translation: AAA85857.1 . Sequence problems.
FO080368 Genomic DNA. Translation: CCD63238.1 .
FO080368 Genomic DNA. Translation: CCD63239.1 .
PIRi E88940.
RefSeqi NP_001021367.1. NM_001026196.4.
NP_503306.1. NM_070905.5.
UniGenei Cel.18040.

3D structure databases

ProteinModelPortali Q10663.
SMRi Q10663. Positions 7-432, 455-963.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 43657. 1 interaction.
DIPi DIP-25113N.
MINTi MINT-1060277.
STRINGi 6239.C05E4.9a.

Proteomic databases

PaxDbi Q10663.
PRIDEi Q10663.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C05E4.9a ; C05E4.9a ; WBGene00001564 . [Q10663-1 ]
C05E4.9b ; C05E4.9b ; WBGene00001564 . [Q10663-2 ]
GeneIDi 178583.
KEGGi cel:CELE_C05E4.9.
UCSCi C05E4.9a. c. elegans. [Q10663-1 ]

Organism-specific databases

CTDi 178583.
WormBasei C05E4.9a ; CE23521 ; WBGene00001564 ; icl-1.
C05E4.9b ; CE32565 ; WBGene00001564 ; icl-1.

Phylogenomic databases

eggNOGi COG2225.
GeneTreei ENSGT00390000002932.
HOGENOMi HOG000112704.
InParanoidi Q10663.
OMAi IWMETSH.
OrthoDBi EOG771267.
PhylomeDBi Q10663.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00719 .
UPA00703 ; UER00720 .

Miscellaneous databases

NextBioi 901736.

Gene expression databases

ExpressionAtlasi Q10663. baseline and differential.

Family and domain databases

Gene3Di 3.20.20.60. 1 hit.
InterProi IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
Pfami PF00463. ICL. 1 hit.
PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51621. SSF51621. 1 hit.
SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01346. isocit_lyase. 1 hit.
TIGR01344. malate_syn_A. 1 hit.
PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle."
    Liu F., Thatcher J.D., Barral J.M., Epstein H.F.
    Dev. Biol. 169:399-414(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DEVELOPMENTAL STAGE, PROTEIN SEQUENCE OF 429-461.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Increased longevity of some C. elegans mitochondrial mutants explained by activation of an alternative energy-producing pathway."
    Gallo M., Park D., Riddle D.L.
    Mech. Ageing Dev. 132:515-518(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiGCP_CAEEL
AccessioniPrimary (citable) accession number: Q10663
Secondary accession number(s): O17353, Q8IA71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 20, 2003
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3