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Q10663

- GCP_CAEEL

UniProt

Q10663 - GCP_CAEEL

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Protein
Bifunctional glyoxylate cycle protein
Gene
gei-7, C05E4.9
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate = succinate + glyoxylate.
Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei601 – 6011Proton acceptor By similarity
Active sitei881 – 8811Proton donor By similarity

GO - Molecular functioni

  1. isocitrate lyase activity Source: WormBase
  2. malate synthase activity Source: WormBase
Complete GO annotation...

GO - Biological processi

  1. determination of adult lifespan Source: WormBase
  2. embryo development Source: WormBase
  3. glyoxylate cycle Source: WormBase
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.
UPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glyoxylate cycle protein
Alternative name(s):
Gex-3-interacting protein 7
Including the following 2 domains:
Isocitrate lyase (EC:4.1.3.1)
Short name:
ICL
Short name:
Isocitrase
Short name:
Isocitratase
Malate synthase (EC:2.3.3.9)
Gene namesi
Name:gei-7
ORF Names:C05E4.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiC05E4.9a; CE23521; WBGene00001564; gei-7.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Bifunctional glyoxylate cycle protein
PRO_0000166874Add
BLAST

Proteomic databases

PaxDbiQ10663.
PRIDEiQ10663.

Expressioni

Tissue specificityi

Intestinal and body wall muscle cells.

Developmental stagei

First detected early in the generation of the clonal E cell lineage that is committed to intestinal development and then increases to maximal levels in actively differentiating intestinal and body wall muscle cells. Highest activity in embryos and falls dramatically during L1 larval development.

Interactioni

Protein-protein interaction databases

BioGridi43657. 1 interaction.
DIPiDIP-25113N.
MINTiMINT-1060277.
STRINGi6239.C05E4.9a.

Structurei

3D structure databases

ProteinModelPortaliQ10663.
SMRiQ10663. Positions 7-432, 455-963.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 443443Isocitrate lyase
Add
BLAST
Regioni444 – 968525Malate synthase
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.
In the C-terminal section; belongs to the malate synthase family.

Phylogenomic databases

eggNOGiCOG2225.
GeneTreeiENSGT00390000002932.
HOGENOMiHOG000112704.
InParanoidiQ10663.
OMAiIWMETSH.
OrthoDBiEOG771267.
PhylomeDBiQ10663.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
PF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
SSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
TIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10663-1 [UniParc]FASTAAdd to Basket

« Hide

MSSAAKNFYQ VVKSAPKGRF KGIKRDYTVE DVLKLRGSID IDYTLATRGA    50
NKLWQLLHTE PFVPALGAQT GNQAVQMVRA GLKAIYLSGW QVAADANSAG 100
DMYPDQSLYP ANSGPELAKR INRSLRRADQ IEACEAEDYL AQRDWYAPIV 150
ADAEAGFGGA LNCFELMKAY IEAGAAGVHY EDQLGSEKKC GHMGGKVLIP 200
TAQHIRHLNA SRLAADVCGV PTIIVARTDA ESSRLLTSDI DPRDHPYIDY 250
EAGRTIEGFY RLKDSTAIQY CIDRAIQYAP YTDLIWMETS HPTIADAREF 300
AEGVHKQYPD KMFAYNCSPS FNWKKHLSPS QMEKFQKELG AMGFKYQFIT 350
LAGYHANSYS MFDLARNYKE KGMLAYSGLQ EGEFAAEKHG YTAVKHQREV 400
GTGYFDAVSR AVTGGLSSTT ALSGSTEEAQ FQTAVASQDE EILSLTAQNV 450
AGDEKILTPD ALRFLHDLNT EFNPRRLRLL SKRNQVQADI NNSLWFPDFN 500
KETEVLRSDQ GWKGAEIPRD LQDRRVEITG PTDRKMVINA MNSGANVFMA 550
DFEDSNSPTW RNQLEGQINL YDAVRNNISY THPTTKKEYT LNEKHAVLKV 600
RPRGWHLPEK HVLIHNQPTS GSLFDFGLFV FHNAKALIAQ GSGPYFYLPK 650
LQSAEEAQLW ADVFKYTEDK LGLARGTIKC TVLIEHLLAS FQLHEIIHAL 700
KDNIVGLNCG RWDYIFSYIK TFQNHRKFLL PDRFQIGMTA PFMRNYSLEV 750
IKACHLRGIH AMGGMAAQIP IKHDQVANDK AFALVRADKE REATDGHDGT 800
WVAHPGLVPL AKRVFDQMMP KPNQISKNLT RANCTKEDLT VIPEGTRTEA 850
GFRHNISVTL GYLDSWLRGT GCVPLYNLME DAATAEISRA QLWQWLHHDA 900
KLEDGRTIDA GLVKQTIAAE TERRLIRAGS VVNRIPEAAD LLEKFVTEEK 950
MSDFLTTDAY DRLVSEGY 968
Length:968
Mass (Da):108,629
Last modified:June 20, 2003 - v2
Checksum:i7BAAF979C1F6D31F
GO

Sequence cautioni

The sequence AAA85857.1 differs from that shown. Reason: Chimeric cDNA.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521D → H in AAA85857. 1 Publication
Sequence conflicti436 – 4361A → R in AAA85857. 1 Publication
Sequence conflicti463 – 4631R → A in AAA85857. 1 Publication
Sequence conflicti957 – 96812TDAYD…VSEGY → DRRLRQACL in AAA85857. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23159 mRNA. Translation: AAA85857.1. Sequence problems.
FO080368 Genomic DNA. Translation: CCD63238.1.
PIRiE88940.
RefSeqiNP_503306.1. NM_070905.5.
UniGeneiCel.18040.

Genome annotation databases

EnsemblMetazoaiC05E4.9a; C05E4.9a; WBGene00001564.
GeneIDi178583.
KEGGicel:CELE_C05E4.9.
UCSCiC05E4.9a. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23159 mRNA. Translation: AAA85857.1 . Sequence problems.
FO080368 Genomic DNA. Translation: CCD63238.1 .
PIRi E88940.
RefSeqi NP_503306.1. NM_070905.5.
UniGenei Cel.18040.

3D structure databases

ProteinModelPortali Q10663.
SMRi Q10663. Positions 7-432, 455-963.
ModBasei Search...

Protein-protein interaction databases

BioGridi 43657. 1 interaction.
DIPi DIP-25113N.
MINTi MINT-1060277.
STRINGi 6239.C05E4.9a.

Proteomic databases

PaxDbi Q10663.
PRIDEi Q10663.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C05E4.9a ; C05E4.9a ; WBGene00001564 .
GeneIDi 178583.
KEGGi cel:CELE_C05E4.9.
UCSCi C05E4.9a. c. elegans.

Organism-specific databases

CTDi 178583.
WormBasei C05E4.9a ; CE23521 ; WBGene00001564 ; gei-7.

Phylogenomic databases

eggNOGi COG2225.
GeneTreei ENSGT00390000002932.
HOGENOMi HOG000112704.
InParanoidi Q10663.
OMAi IWMETSH.
OrthoDBi EOG771267.
PhylomeDBi Q10663.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00719 .
UPA00703 ; UER00720 .

Miscellaneous databases

NextBioi 901736.

Family and domain databases

Gene3Di 3.20.20.60. 1 hit.
InterProi IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
Pfami PF00463. ICL. 1 hit.
PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51621. SSF51621. 1 hit.
SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01346. isocit_lyase. 1 hit.
TIGR01344. malate_syn_A. 1 hit.
PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle."
    Liu F., Thatcher J.D., Barral J.M., Epstein H.F.
    Dev. Biol. 169:399-414(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 429-461.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiGCP_CAEEL
AccessioniPrimary (citable) accession number: Q10663
Secondary accession number(s): O17353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 20, 2003
Last modified: June 11, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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