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Q10663 (GCP_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional glyoxylate cycle protein
Alternative name(s):
Gex-3-interacting protein 7

Including the following 2 domains:

  1. Isocitrate lyase
    Short name=ICL
    Short name=Isocitrase
    Short name=Isocitratase
    EC=4.1.3.1
  2. Malate synthase
    EC=2.3.3.9
Gene names
Name:gei-7
ORF Names:C05E4.9
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length968 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate = succinate + glyoxylate.

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Tissue specificity

Intestinal and body wall muscle cells.

Developmental stage

First detected early in the generation of the clonal E cell lineage that is committed to intestinal development and then increases to maximal levels in actively differentiating intestinal and body wall muscle cells. Highest activity in embryos and falls dramatically during L1 larval development.

Sequence similarities

In the N-terminal section; belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

In the C-terminal section; belongs to the malate synthase family.

Sequence caution

The sequence AAA85857.1 differs from that shown. Reason: Chimeric cDNA.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 968968Bifunctional glyoxylate cycle protein
PRO_0000166874

Regions

Region1 – 443443Isocitrate lyase
Region444 – 968525Malate synthase

Sites

Active site6011Proton acceptor By similarity
Active site8811Proton donor By similarity

Experimental info

Sequence conflict1521D → H in AAA85857. Ref.1
Sequence conflict4361A → R in AAA85857. Ref.1
Sequence conflict4631R → A in AAA85857. Ref.1
Sequence conflict957 – 96812TDAYD…VSEGY → DRRLRQACL in AAA85857. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q10663 [UniParc].

Last modified June 20, 2003. Version 2.
Checksum: 7BAAF979C1F6D31F

FASTA968108,629
        10         20         30         40         50         60 
MSSAAKNFYQ VVKSAPKGRF KGIKRDYTVE DVLKLRGSID IDYTLATRGA NKLWQLLHTE 

        70         80         90        100        110        120 
PFVPALGAQT GNQAVQMVRA GLKAIYLSGW QVAADANSAG DMYPDQSLYP ANSGPELAKR 

       130        140        150        160        170        180 
INRSLRRADQ IEACEAEDYL AQRDWYAPIV ADAEAGFGGA LNCFELMKAY IEAGAAGVHY 

       190        200        210        220        230        240 
EDQLGSEKKC GHMGGKVLIP TAQHIRHLNA SRLAADVCGV PTIIVARTDA ESSRLLTSDI 

       250        260        270        280        290        300 
DPRDHPYIDY EAGRTIEGFY RLKDSTAIQY CIDRAIQYAP YTDLIWMETS HPTIADAREF 

       310        320        330        340        350        360 
AEGVHKQYPD KMFAYNCSPS FNWKKHLSPS QMEKFQKELG AMGFKYQFIT LAGYHANSYS 

       370        380        390        400        410        420 
MFDLARNYKE KGMLAYSGLQ EGEFAAEKHG YTAVKHQREV GTGYFDAVSR AVTGGLSSTT 

       430        440        450        460        470        480 
ALSGSTEEAQ FQTAVASQDE EILSLTAQNV AGDEKILTPD ALRFLHDLNT EFNPRRLRLL 

       490        500        510        520        530        540 
SKRNQVQADI NNSLWFPDFN KETEVLRSDQ GWKGAEIPRD LQDRRVEITG PTDRKMVINA 

       550        560        570        580        590        600 
MNSGANVFMA DFEDSNSPTW RNQLEGQINL YDAVRNNISY THPTTKKEYT LNEKHAVLKV 

       610        620        630        640        650        660 
RPRGWHLPEK HVLIHNQPTS GSLFDFGLFV FHNAKALIAQ GSGPYFYLPK LQSAEEAQLW 

       670        680        690        700        710        720 
ADVFKYTEDK LGLARGTIKC TVLIEHLLAS FQLHEIIHAL KDNIVGLNCG RWDYIFSYIK 

       730        740        750        760        770        780 
TFQNHRKFLL PDRFQIGMTA PFMRNYSLEV IKACHLRGIH AMGGMAAQIP IKHDQVANDK 

       790        800        810        820        830        840 
AFALVRADKE REATDGHDGT WVAHPGLVPL AKRVFDQMMP KPNQISKNLT RANCTKEDLT 

       850        860        870        880        890        900 
VIPEGTRTEA GFRHNISVTL GYLDSWLRGT GCVPLYNLME DAATAEISRA QLWQWLHHDA 

       910        920        930        940        950        960 
KLEDGRTIDA GLVKQTIAAE TERRLIRAGS VVNRIPEAAD LLEKFVTEEK MSDFLTTDAY 


DRLVSEGY 

« Hide

References

« Hide 'large scale' references
[1]"Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle."
Liu F., Thatcher J.D., Barral J.M., Epstein H.F.
Dev. Biol. 169:399-414(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 429-461.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U23159 mRNA. Translation: AAA85857.1. Sequence problems.
FO080368 Genomic DNA. Translation: CCD63238.1.
PIRE88940.
RefSeqNP_503306.1. NM_070905.5.
UniGeneCel.18040.

3D structure databases

ProteinModelPortalQ10663.
SMRQ10663. Positions 7-432, 455-963.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid43657. 1 interaction.
DIPDIP-25113N.
MINTMINT-1060277.
STRING6239.C05E4.9a.

Proteomic databases

PaxDbQ10663.
PRIDEQ10663.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC05E4.9a; C05E4.9a; WBGene00001564.
GeneID178583.
KEGGcel:CELE_C05E4.9.
UCSCC05E4.9a. c. elegans.

Organism-specific databases

CTD178583.
WormBaseC05E4.9a; CE23521; WBGene00001564; gei-7.

Phylogenomic databases

eggNOGCOG2225.
GeneTreeENSGT00390000002932.
HOGENOMHOG000112704.
InParanoidQ10663.
OMAIWMETSH.
OrthoDBEOG771267.
PhylomeDBQ10663.

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.
UPA00703; UER00720.

Family and domain databases

Gene3D3.20.20.60. 1 hit.
InterProIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF00463. ICL. 1 hit.
PF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51621. SSF51621. 1 hit.
SSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01346. isocit_lyase. 1 hit.
TIGR01344. malate_syn_A. 1 hit.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio901736.

Entry information

Entry nameGCP_CAEEL
AccessionPrimary (citable) accession number: Q10663
Secondary accession number(s): O17353
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 20, 2003
Last modified: June 11, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase