Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q10663

- GCP_CAEEL

UniProt

Q10663 - GCP_CAEEL

Protein

Bifunctional glyoxylate cycle protein

Gene

icl-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (20 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation
    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei601 – 6011Proton acceptorPROSITE-ProRule annotation
    Active sitei881 – 8811Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. isocitrate lyase activity Source: WormBase
    2. malate synthase activity Source: WormBase

    GO - Biological processi

    1. determination of adult lifespan Source: WormBase
    2. embryo development Source: WormBase
    3. glyoxylate cycle Source: WormBase
    4. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00719.
    UPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional glyoxylate cycle protein
    Alternative name(s):
    Gex-3-interacting protein 7
    Including the following 2 domains:
    Isocitrate lyase (EC:4.1.3.1)
    Short name:
    ICL
    Short name:
    Isocitrase
    Short name:
    Isocitratase
    Malate synthase (EC:2.3.3.9)
    Gene namesi
    Name:icl-1
    Synonyms:gei-7
    ORF Names:C05E4.9
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiC05E4.9a; CE23521; WBGene00001564; icl-1.
    C05E4.9b; CE32565; WBGene00001564; icl-1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 968968Bifunctional glyoxylate cycle proteinPRO_0000166874Add
    BLAST

    Proteomic databases

    PaxDbiQ10663.
    PRIDEiQ10663.

    Expressioni

    Tissue specificityi

    Intestinal and body wall muscle cells.

    Developmental stagei

    First detected early in the generation of the clonal E cell lineage that is committed to intestinal development and then increases to maximal levels in actively differentiating intestinal and body wall muscle cells. Highest activity in embryos and falls dramatically during L1 larval development (PubMed:7781887). Expression is highest in L3 larval stage with low levels at other stages (PubMed:21884719).2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi43657. 1 interaction.
    DIPiDIP-25113N.
    MINTiMINT-1060277.
    STRINGi6239.C05E4.9a.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10663.
    SMRiQ10663. Positions 7-432, 455-963.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 443443Isocitrate lyaseAdd
    BLAST
    Regioni444 – 968525Malate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.Curated
    In the C-terminal section; belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    GeneTreeiENSGT00390000002932.
    HOGENOMiHOG000112704.
    InParanoidiQ10663.
    OMAiIWMETSH.
    OrthoDBiEOG771267.
    PhylomeDBiQ10663.

    Family and domain databases

    Gene3Di3.20.20.60. 1 hit.
    InterProiIPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF00463. ICL. 1 hit.
    PF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51621. SSF51621. 1 hit.
    SSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
    TIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
    PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q10663-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSAAKNFYQ VVKSAPKGRF KGIKRDYTVE DVLKLRGSID IDYTLATRGA    50
    NKLWQLLHTE PFVPALGAQT GNQAVQMVRA GLKAIYLSGW QVAADANSAG 100
    DMYPDQSLYP ANSGPELAKR INRSLRRADQ IEACEAEDYL AQRDWYAPIV 150
    ADAEAGFGGA LNCFELMKAY IEAGAAGVHY EDQLGSEKKC GHMGGKVLIP 200
    TAQHIRHLNA SRLAADVCGV PTIIVARTDA ESSRLLTSDI DPRDHPYIDY 250
    EAGRTIEGFY RLKDSTAIQY CIDRAIQYAP YTDLIWMETS HPTIADAREF 300
    AEGVHKQYPD KMFAYNCSPS FNWKKHLSPS QMEKFQKELG AMGFKYQFIT 350
    LAGYHANSYS MFDLARNYKE KGMLAYSGLQ EGEFAAEKHG YTAVKHQREV 400
    GTGYFDAVSR AVTGGLSSTT ALSGSTEEAQ FQTAVASQDE EILSLTAQNV 450
    AGDEKILTPD ALRFLHDLNT EFNPRRLRLL SKRNQVQADI NNSLWFPDFN 500
    KETEVLRSDQ GWKGAEIPRD LQDRRVEITG PTDRKMVINA MNSGANVFMA 550
    DFEDSNSPTW RNQLEGQINL YDAVRNNISY THPTTKKEYT LNEKHAVLKV 600
    RPRGWHLPEK HVLIHNQPTS GSLFDFGLFV FHNAKALIAQ GSGPYFYLPK 650
    LQSAEEAQLW ADVFKYTEDK LGLARGTIKC TVLIEHLLAS FQLHEIIHAL 700
    KDNIVGLNCG RWDYIFSYIK TFQNHRKFLL PDRFQIGMTA PFMRNYSLEV 750
    IKACHLRGIH AMGGMAAQIP IKHDQVANDK AFALVRADKE REATDGHDGT 800
    WVAHPGLVPL AKRVFDQMMP KPNQISKNLT RANCTKEDLT VIPEGTRTEA 850
    GFRHNISVTL GYLDSWLRGT GCVPLYNLME DAATAEISRA QLWQWLHHDA 900
    KLEDGRTIDA GLVKQTIAAE TERRLIRAGS VVNRIPEAAD LLEKFVTEEK 950
    MSDFLTTDAY DRLVSEGY 968
    Length:968
    Mass (Da):108,629
    Last modified:June 20, 2003 - v2
    Checksum:i7BAAF979C1F6D31F
    GO
    Isoform b (identifier: Q10663-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         862-863: YL → EP
         864-968: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:863
    Mass (Da):96,743
    Checksum:i5392FF3F102D0903
    GO

    Sequence cautioni

    The sequence AAA85857.1 differs from that shown. Reason: Chimeric cDNA.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 1521D → H in AAA85857. (PubMed:7781887)Curated
    Sequence conflicti436 – 4361A → R in AAA85857. (PubMed:7781887)Curated
    Sequence conflicti463 – 4631R → A in AAA85857. (PubMed:7781887)Curated
    Sequence conflicti957 – 96812TDAYD…VSEGY → DRRLRQACL in AAA85857. (PubMed:7781887)CuratedAdd
    BLAST

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei862 – 8632YL → EP in isoform b. CuratedVSP_056035
    Alternative sequencei864 – 968105Missing in isoform b. CuratedVSP_056036Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23159 mRNA. Translation: AAA85857.1. Sequence problems.
    FO080368 Genomic DNA. Translation: CCD63238.1.
    FO080368 Genomic DNA. Translation: CCD63239.1.
    PIRiE88940.
    RefSeqiNP_001021367.1. NM_001026196.4.
    NP_503306.1. NM_070905.5.
    UniGeneiCel.18040.

    Genome annotation databases

    EnsemblMetazoaiC05E4.9a; C05E4.9a; WBGene00001564.
    C05E4.9b; C05E4.9b; WBGene00001564.
    GeneIDi178583.
    KEGGicel:CELE_C05E4.9.
    UCSCiC05E4.9a. c. elegans.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23159 mRNA. Translation: AAA85857.1 . Sequence problems.
    FO080368 Genomic DNA. Translation: CCD63238.1 .
    FO080368 Genomic DNA. Translation: CCD63239.1 .
    PIRi E88940.
    RefSeqi NP_001021367.1. NM_001026196.4.
    NP_503306.1. NM_070905.5.
    UniGenei Cel.18040.

    3D structure databases

    ProteinModelPortali Q10663.
    SMRi Q10663. Positions 7-432, 455-963.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 43657. 1 interaction.
    DIPi DIP-25113N.
    MINTi MINT-1060277.
    STRINGi 6239.C05E4.9a.

    Proteomic databases

    PaxDbi Q10663.
    PRIDEi Q10663.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C05E4.9a ; C05E4.9a ; WBGene00001564 .
    C05E4.9b ; C05E4.9b ; WBGene00001564 .
    GeneIDi 178583.
    KEGGi cel:CELE_C05E4.9.
    UCSCi C05E4.9a. c. elegans.

    Organism-specific databases

    CTDi 178583.
    WormBasei C05E4.9a ; CE23521 ; WBGene00001564 ; icl-1.
    C05E4.9b ; CE32565 ; WBGene00001564 ; icl-1.

    Phylogenomic databases

    eggNOGi COG2225.
    GeneTreei ENSGT00390000002932.
    HOGENOMi HOG000112704.
    InParanoidi Q10663.
    OMAi IWMETSH.
    OrthoDBi EOG771267.
    PhylomeDBi Q10663.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00719 .
    UPA00703 ; UER00720 .

    Miscellaneous databases

    NextBioi 901736.

    Family and domain databases

    Gene3Di 3.20.20.60. 1 hit.
    InterProi IPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF00463. ICL. 1 hit.
    PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51621. SSF51621. 1 hit.
    SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01346. isocit_lyase. 1 hit.
    TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
    PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle."
      Liu F., Thatcher J.D., Barral J.M., Epstein H.F.
      Dev. Biol. 169:399-414(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DEVELOPMENTAL STAGE, PROTEIN SEQUENCE OF 429-461.
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "Increased longevity of some C. elegans mitochondrial mutants explained by activation of an alternative energy-producing pathway."
      Gallo M., Park D., Riddle D.L.
      Mech. Ageing Dev. 132:515-518(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiGCP_CAEEL
    AccessioniPrimary (citable) accession number: Q10663
    Secondary accession number(s): O17353, Q8IA71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 20, 2003
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3