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Protein

Triosephosphate isomerase

Gene

tpi-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpi-1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10SubstrateBy similarity1
Binding sitei12SubstrateBy similarity1
Active sitei94ElectrophileBy similarity1
Active sitei164Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: WormBase
  • determination of adult lifespan Source: WormBase
  • gluconeogenesis Source: GO_Central
  • glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
  • glycerol catabolic process Source: GO_Central
  • glycolytic process Source: GO_Central
  • pentose-phosphate shunt Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BRENDAi5.3.1.1. 1045.
ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:tpi-1
ORF Names:Y17G7B.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiY17G7B.7; CE19040; WBGene00006601; tpi-1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • intracellular Source: WormBase
  • myofibril Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000901271 – 247Triosephosphate isomeraseAdd BLAST247

Proteomic databases

EPDiQ10657.
PaxDbiQ10657.
PeptideAtlasiQ10657.
PRIDEiQ10657.

2D gel databases

World-2DPAGE0020:Q10657.

Expressioni

Gene expression databases

BgeeiWBGene00006601.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi6239.Y17G7B.7.2.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi17 – 29Combined sources13
Beta strandi36 – 41Combined sources6
Helixi44 – 46Combined sources3
Helixi47 – 53Combined sources7
Beta strandi58 – 63Combined sources6
Beta strandi67 – 72Combined sources6
Helixi79 – 84Combined sources6
Beta strandi89 – 93Combined sources5
Helixi95 – 99Combined sources5
Helixi105 – 117Combined sources13
Beta strandi121 – 126Combined sources6
Helixi130 – 134Combined sources5
Helixi138 – 150Combined sources13
Turni151 – 153Combined sources3
Beta strandi159 – 163Combined sources5
Helixi166 – 168Combined sources3
Turni169 – 171Combined sources3
Helixi177 – 194Combined sources18
Helixi197 – 202Combined sources6
Beta strandi205 – 210Combined sources6
Turni213 – 215Combined sources3
Helixi216 – 219Combined sources4
Beta strandi227 – 231Combined sources5
Helixi232 – 235Combined sources4
Helixi238 – 246Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MO0X-ray1.70A/B2-247[»]
ProteinModelPortaliQ10657.
SMRiQ10657.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ10657.

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
InParanoidiQ10657.
KOiK01803.
OMAiIEKNGTM.
OrthoDBiEOG091G0LEZ.
PhylomeDBiQ10657.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRKFFVGGN WKMNGDYASV DGIVTFLNAS ADNSSVDVVV APPAPYLAYA
60 70 80 90 100
KSKLKAGVLV AAQNCYKVPK GAFTGEISPA MIKDLGLEWV ILGHSERRHV
110 120 130 140 150
FGESDALIAE KTVHALEAGI KVVFCIGEKL EEREAGHTKD VNFRQLQAIV
160 170 180 190 200
DKGVSWENIV IAYEPVWAIG TGKTASGEQA QEVHEWIRAF LKEKVSPAVA
210 220 230 240
DATRIIYGGS VTADNAAELG KKPDIDGFLV GGASLKPDFV KIINARS
Length:247
Mass (Da):26,574
Last modified:June 20, 2002 - v2
Checksum:iB4ADF85D0D1A37EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti218 – 219EL → DV in AAA79846 (PubMed:7667320).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23081 Genomic DNA. Translation: AAA79846.1.
AL023828 Genomic DNA. Translation: CAA19447.1.
PIRiT26493.
RefSeqiNP_496563.1. NM_064162.4.
UniGeneiCel.6518.

Genome annotation databases

EnsemblMetazoaiY17G7B.7; Y17G7B.7; WBGene00006601.
GeneIDi174844.
KEGGicel:CELE_Y17G7B.7.
UCSCiY17G7B.7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23081 Genomic DNA. Translation: AAA79846.1.
AL023828 Genomic DNA. Translation: CAA19447.1.
PIRiT26493.
RefSeqiNP_496563.1. NM_064162.4.
UniGeneiCel.6518.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MO0X-ray1.70A/B2-247[»]
ProteinModelPortaliQ10657.
SMRiQ10657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.Y17G7B.7.2.

2D gel databases

World-2DPAGE0020:Q10657.

Proteomic databases

EPDiQ10657.
PaxDbiQ10657.
PeptideAtlasiQ10657.
PRIDEiQ10657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY17G7B.7; Y17G7B.7; WBGene00006601.
GeneIDi174844.
KEGGicel:CELE_Y17G7B.7.
UCSCiY17G7B.7.1. c. elegans.

Organism-specific databases

CTDi174844.
WormBaseiY17G7B.7; CE19040; WBGene00006601; tpi-1.

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
InParanoidiQ10657.
KOiK01803.
OMAiIEKNGTM.
OrthoDBiEOG091G0LEZ.
PhylomeDBiQ10657.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
BRENDAi5.3.1.1. 1045.
ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Miscellaneous databases

EvolutionaryTraceiQ10657.
PROiQ10657.

Gene expression databases

BgeeiWBGene00006601.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPIS_CAEEL
AccessioniPrimary (citable) accession number: Q10657
Secondary accession number(s): Q9U2R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.