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Protein

Triosephosphate isomerase

Gene

tpi-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpi-1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101SubstrateBy similarity
Binding sitei12 – 121SubstrateBy similarity
Active sitei94 – 941ElectrophileBy similarity
Active sitei164 – 1641Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: WormBase
  • determination of adult lifespan Source: WormBase
  • gluconeogenesis Source: UniProtKB-UniPathway
  • glycolytic process Source: UniProtKB-UniPathway
  • pentose-phosphate shunt Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BRENDAi5.3.1.1. 1045.
ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:tpi-1
ORF Names:Y17G7B.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiY17G7B.7; CE19040; WBGene00006601; tpi-1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: WormBase
  • myofibril Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247Triosephosphate isomerasePRO_0000090127Add
BLAST

Proteomic databases

EPDiQ10657.
PaxDbiQ10657.
PRIDEiQ10657.

2D gel databases

World-2DPAGE0020:Q10657.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi6239.Y17G7B.7.2.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Helixi17 – 2913Combined sources
Beta strandi36 – 416Combined sources
Helixi44 – 463Combined sources
Helixi47 – 537Combined sources
Beta strandi58 – 636Combined sources
Beta strandi67 – 726Combined sources
Helixi79 – 846Combined sources
Beta strandi89 – 935Combined sources
Helixi95 – 995Combined sources
Helixi105 – 11713Combined sources
Beta strandi121 – 1266Combined sources
Helixi130 – 1345Combined sources
Helixi138 – 15013Combined sources
Turni151 – 1533Combined sources
Beta strandi159 – 1635Combined sources
Helixi166 – 1683Combined sources
Turni169 – 1713Combined sources
Helixi177 – 19418Combined sources
Helixi197 – 2026Combined sources
Beta strandi205 – 2106Combined sources
Turni213 – 2153Combined sources
Helixi216 – 2194Combined sources
Beta strandi227 – 2315Combined sources
Helixi232 – 2354Combined sources
Helixi238 – 2469Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MO0X-ray1.70A/B2-247[»]
ProteinModelPortaliQ10657.
SMRiQ10657. Positions 3-247.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ10657.

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
InParanoidiQ10657.
KOiK01803.
OMAiIEKNGTM.
PhylomeDBiQ10657.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRKFFVGGN WKMNGDYASV DGIVTFLNAS ADNSSVDVVV APPAPYLAYA
60 70 80 90 100
KSKLKAGVLV AAQNCYKVPK GAFTGEISPA MIKDLGLEWV ILGHSERRHV
110 120 130 140 150
FGESDALIAE KTVHALEAGI KVVFCIGEKL EEREAGHTKD VNFRQLQAIV
160 170 180 190 200
DKGVSWENIV IAYEPVWAIG TGKTASGEQA QEVHEWIRAF LKEKVSPAVA
210 220 230 240
DATRIIYGGS VTADNAAELG KKPDIDGFLV GGASLKPDFV KIINARS
Length:247
Mass (Da):26,574
Last modified:June 20, 2002 - v2
Checksum:iB4ADF85D0D1A37EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti218 – 2192EL → DV in AAA79846 (PubMed:7667320).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23081 Genomic DNA. Translation: AAA79846.1.
AL023828 Genomic DNA. Translation: CAA19447.1.
PIRiT26493.
RefSeqiNP_496563.1. NM_064162.4.
UniGeneiCel.6518.

Genome annotation databases

EnsemblMetazoaiY17G7B.7; Y17G7B.7; WBGene00006601.
GeneIDi174844.
KEGGicel:CELE_Y17G7B.7.
UCSCiY17G7B.7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23081 Genomic DNA. Translation: AAA79846.1.
AL023828 Genomic DNA. Translation: CAA19447.1.
PIRiT26493.
RefSeqiNP_496563.1. NM_064162.4.
UniGeneiCel.6518.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MO0X-ray1.70A/B2-247[»]
ProteinModelPortaliQ10657.
SMRiQ10657. Positions 3-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.Y17G7B.7.2.

2D gel databases

World-2DPAGE0020:Q10657.

Proteomic databases

EPDiQ10657.
PaxDbiQ10657.
PRIDEiQ10657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY17G7B.7; Y17G7B.7; WBGene00006601.
GeneIDi174844.
KEGGicel:CELE_Y17G7B.7.
UCSCiY17G7B.7.1. c. elegans.

Organism-specific databases

CTDi174844.
WormBaseiY17G7B.7; CE19040; WBGene00006601; tpi-1.

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
InParanoidiQ10657.
KOiK01803.
OMAiIEKNGTM.
PhylomeDBiQ10657.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
BRENDAi5.3.1.1. 1045.
ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Miscellaneous databases

EvolutionaryTraceiQ10657.
PROiQ10657.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Seven newly discovered intron positions in the triose-phosphate isomerase gene: evidence for the introns-late theory."
    Logsdon J.M. Jr., Tyshenko M.G., Dixon C., D-Jafari J., Walker V.K., Palmer J.D.
    Proc. Natl. Acad. Sci. U.S.A. 92:8507-8511(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Structural genomics of Caenorhabditis elegans: triosephosphate isomerase."
    Symersky J., Li S., Carson M., Luo M.
    Proteins 51:484-486(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 2-247, HOMODIMERIZATION.

Entry informationi

Entry nameiTPIS_CAEEL
AccessioniPrimary (citable) accession number: Q10657
Secondary accession number(s): Q9U2R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: June 8, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.