Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q10656 (EGL15_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myoblast growth factor receptor egl-15

EC=2.7.10.1
Alternative name(s):
Egg-laying defective protein 15
Gene names
Name:egl-15
ORF Names:F58A3.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1040 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activation leads to the phosphorylation of SOC-1 on Tyr-408 triggering PTP-2 recruitment and/or activation. Activates the SEM-5/SOS-1/RAS/MAPK pathway. Acts in the hypodermis to regulate axon growth. Isoform 2has no effect on axon growth but affects the maintenance of axon position. Isoform b acts as a receptor for an extracellular signal required for the guidance of sex myoblast migration during gonad development. Isoforms 1, 3, 4 and 5 appear to play a role in maintaining body morphology at higher temperatures. Ref.1 Ref.2 Ref.4

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactor

Manganese By similarity.

Subunit structure

Interaction of egl-17 with isoform b is required for sex myoblast chemoattraction. Isoforms a, c, d and e interact with let-756 and play a role in the regulation of temperature-dependent growth.

Subcellular location

Membrane; Single-pass type I membrane protein.

Developmental stage

Expressed in the hypodermal membrane during the larval stage. Ref.4

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   LigandATP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Receptor
Transferase
Tyrosine-protein kinase
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell migration

Inferred from mutant phenotype Ref.1. Source: WormBase

digestive tract morphogenesis

Inferred from mutant phenotype PubMed 20004187. Source: WormBase

fibroblast growth factor receptor signaling pathway

Inferred from mutant phenotype Ref.1. Source: WormBase

muscle organ development

Inferred from mutant phenotype PubMed 16495308. Source: WormBase

nematode larval development

Inferred from mutant phenotype Ref.4. Source: WormBase

oviposition

Inferred from mutant phenotype Ref.4. Source: WormBase

protein phosphorylation

Inferred from direct assay PubMed 20004187. Source: WormBase

regulation of cell projection organization

Inferred from mutant phenotype PubMed 16495308. Source: WormBase

   Cellular_componentcell

Inferred from direct assay PubMed 9463355. Source: WormBase

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein kinase activity

Inferred from direct assay PubMed 20004187. Source: WormBase

transmembrane receptor protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q10656-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q10656-2)

Also known as: EGL-15(5A);

The sequence of this isoform differs from the canonical sequence as follows:
     129-245: FCDYFLFPDI...DSEESPSESR → PLKLFDWLTE...RLRQINHALG
Isoform c (identifier: Q10656-3)

The sequence of this isoform differs from the canonical sequence as follows:
     829-829: R → RL
     984-984: E → EVDQN
     1027-1040: RIPSNNNSMSKPEF → LYIHKVLNEP...NPLPTKETIV
Isoform d (identifier: Q10656-4)

The sequence of this isoform differs from the canonical sequence as follows:
     829-829: R → RL
     984-984: E → EVDQN
     1027-1040: RIPSNNNSMSKPEF → LYIHKVLNEP...DDEKHHYYYS
Isoform e (identifier: Q10656-5)

The sequence of this isoform differs from the canonical sequence as follows:
     829-829: R → RL
     984-984: E → EVDQN
     1027-1040: RIPSNNNSMSKPEF → LYIHKVLNEPIGNGSNSPVL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 10401021Myoblast growth factor receptor egl-15
PRO_0000016796

Regions

Topological domain20 – 525506Extracellular Potential
Transmembrane526 – 54924Helical; Potential
Topological domain550 – 1040491Cytoplasmic Potential
Domain33 – 12593Ig-like C2-type 1
Domain287 – 38397Ig-like C2-type 2
Domain391 – 501111Ig-like C2-type 3
Domain640 – 931292Protein kinase
Nucleotide binding646 – 6549ATP By similarity

Sites

Active site7971Proton acceptor By similarity
Binding site6721ATP By similarity

Amino acid modifications

Modified residue8281Phosphotyrosine; by autocatalysis By similarity
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Glycosylation4011N-linked (GlcNAc...) Potential
Glycosylation4071N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Glycosylation4401N-linked (GlcNAc...) Potential
Glycosylation4491N-linked (GlcNAc...) Potential
Glycosylation4741N-linked (GlcNAc...) Potential
Glycosylation4971N-linked (GlcNAc...) Potential
Disulfide bond55 ↔ 109 Potential
Disulfide bond314 ↔ 367 Potential
Disulfide bond414 ↔ 485 Potential

Natural variations

Alternative sequence129 – 245117FCDYF…PSESR → PLKLFDWLTEHRVFDISHLL PKLLPPAEMRRVKSQLGGWE KMNNEQKIVRARHILRLRQI NHALG in isoform b.
VSP_019861
Alternative sequence8291R → RL in isoform c, isoform d and isoform e.
VSP_002991
Alternative sequence9841E → EVDQN in isoform c, isoform d and isoform e.
VSP_002992
Alternative sequence1027 – 104014RIPSN…SKPEF → LYIHKVLNEPIGNGYVRQDK LARAVSGVANQSLDSALGSP AWPSYDRPSNKASCLDQTHQ YYNTTSKIQYLHFTFDDPDC MTRSRDSAIFEESYHPNYIQ SHPLYSKIIIKKNMTPRNPL PTKETIV in isoform c.
VSP_002994
Alternative sequence1027 – 104014RIPSN…SKPEF → LYIHKVLNEPIGNGYVRQDK LARAVSGVANQSLDSALGSP AWPSYDRPSNKASCLDDEKH HYYYS in isoform d.
VSP_019862
Alternative sequence1027 – 104014RIPSN…SKPEF → LYIHKVLNEPIGNGSNSPVL in isoform e.
VSP_019863

Experimental info

Mutagenesis6801E → K in N1775; loss of activity. Ref.1
Mutagenesis7141P → L in N1783; loss of activity. Ref.1
Mutagenesis9301W → * in N1477ts; loss of activity. Ref.1
Sequence conflict5511Q → G in AAP44084. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 8CA227195DDBCD69

FASTA1,040118,956
        10         20         30         40         50         60 
MSYFLASCLG VGLLSTVSCS LQGLTSHYRE NIPRFKHVAN ERYEVFLGDE IKFDCQTAAS 

        70         80         90        100        110        120 
KISAFVEWYR NDKLLKNDQI DKDKIRKDNN RMMLHLKNID VSDQGLWSCR VHNAYGQISR 

       130        140        150        160        170        180 
NFTVEVIDFC DYFLFPDIHH LNIPMECVCL WKYNKEAKRS DVNYAAVTGE VCSKYASRMI 

       190        200        210        220        230        240 
NRARKPLPMI PCFGDHCKEF DTTPVSDFGL PGKPEDDPLV KRVVLKKDDV IVPVHDSEES 

       250        260        270        280        290        300 
PSESRTEFIN ADEKENKEDE EEDYSVSQPV APDAGLTELN ITAEEPPYFK SNDNIVLFNE 

       310        320        330        340        350        360 
THALPAGRTL KLNCRAKGYP EPQIIWYKNG KMLKKSSARS GGYEFKFNRW SLEVEDAVVA 

       370        380        390        400        410        420 
DSGEFHCEAL NKVGSAKKYF HVIIVNRMRR PPIIVPNILA NQSVNINDTA TFHCKVVSDL 

       430        440        450        460        470        480 
LPHIIWVRIN KINGSYSYYN NSAEEYMFNY TEMDTFDKAH VHHVGDESTL TIFNVSLDDQ 

       490        500        510        520        530        540 
GIYACLSGNS LGMSMANATL TVNEFMAIHL LTGDEPKIDR WTTSDYIFTT ILLFLLLAAT 

       550        560        570        580        590        600 
LFGILFMVCK QTLHKKGFMD DTVGLVARKK RVVVSKRPMN EDNENSDDEP SPYQIQIIET 

       610        620        630        640        650        660 
PITKKEAARK QRKRMNSENT VLSEYEVDSD PVWEVERSKL SLVHMLGEGA FGEVWKATYK 

       670        680        690        700        710        720 
ETENNEIAVA VKKLKMSAHE KELIDLVSEM ETFKVIGEHE NVLRLIGCCT GAGPLYVVVE 

       730        740        750        760        770        780 
LCKHGNLRDF LRAHRPKEEK AKKSSQELTD YLEPRKASDK DDIELIPNLT QRHLVQFAWQ 

       790        800        810        820        830        840 
VAQGMNFLAS KKIIHRDLAA RNVLVGDGHV LKISDFGLSR DVHCNDYYRK RGNGRLPIKW 

       850        860        870        880        890        900 
MALEALDSNV YTVESDVWSY GVLLWEIMTL GGTPYPTIAM PELYANLKEG YRMEPPHLCP 

       910        920        930        940        950        960 
QEVYHLMCSC WREKLEERPS FKTIVDYLDW MLTMTNETIE GSQEFNDQFF SERSTASGPV 

       970        980        990       1000       1010       1020 
SPMESFQKKR KHRPLSAPVN LPSEPQHTIC DDYESNFSVE PPNDPNHLYC NDNMLKNHII 

      1030       1040 
TPETSQRIPS NNNSMSKPEF 

« Hide

Isoform b (EGL-15(5A)) [UniParc].

Checksum: A7365D218C082E49
Show »

FASTA988113,327
Isoform c [UniParc].

Checksum: 2159D2E7C6564685
Show »

FASTA1,158132,425
Isoform d [UniParc].

Checksum: C76B0B8A20852F80
Show »

FASTA1,096125,172
Isoform e [UniParc].

Checksum: A9616D4CAEA14AEE
Show »

FASTA1,051120,070

References

« Hide 'large scale' references
[1]"An FGF receptor signaling pathway is required for the normal cell migrations of the sex myoblasts in C. elegans hermaphrodites."
Devore D.L., Horvitz H.R., Stern M.J.
Cell 83:611-620(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, MUTAGENESIS OF GLU-680; PRO-714 AND TRP-930.
Strain: Bristol N2.
[2]"Alternative splicing affecting a novel domain in the C. elegans EGL-15 FGF receptor confers functional specificity."
Goodman S.J., Branda C.S., Robinson M.K., Burdine R.D., Stern M.J.
Development 130:3757-3766(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), NUCLEOTIDE SEQUENCE [MRNA] OF 932-1040 (ISOFORMS A; C; D AND E), FUNCTION, INTERACTION WITH EGL-17 AND LET-756.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[4]"The Caenorhabditis elegans EGL-15 signaling pathway implicates a DOS-like multisubstrate adaptor protein in fibroblast growth factor signal transduction."
Schutzman J.L., Borland C.Z., Newman J.C., Robinson M.K., Kokel M., Stern M.J.
Mol. Cell. Biol. 21:8104-8116(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39761 mRNA. Translation: AAC46934.1.
AY268435 mRNA. Translation: AAP31029.1.
AY268436 mRNA. Translation: AAP31030.1.
AY288941 mRNA. Translation: AAP44084.1.
AY288942 mRNA. Translation: AAP44085.1.
AY292532 mRNA. Translation: AAP74805.1.
Z81017 Genomic DNA. Translation: CAB02673.2.
Z81017 Genomic DNA. Translation: CAC70094.2.
Z81017 Genomic DNA. Translation: CAC70095.2.
Z81017 Genomic DNA. Translation: CAD44136.1.
Z81017 Genomic DNA. Translation: CAE47468.1.
PIRA57638.
T22889.
RefSeqNP_001024723.1. NM_001029552.2. [Q10656-2]
NP_001024724.2. NM_001029553.4. [Q10656-3]
NP_001024725.1. NM_001029554.3. [Q10656-4]
NP_001024726.1. NM_001029555.3. [Q10656-5]
NP_509842.2. NM_077441.6. [Q10656-1]
UniGeneCel.17140.

3D structure databases

ProteinModelPortalQ10656.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid46202. 148 interactions.
IntActQ10656. 1 interaction.

Proteomic databases

PaxDbQ10656.
PRIDEQ10656.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF58A3.2a.1; F58A3.2a.1; WBGene00001184. [Q10656-1]
F58A3.2a.2; F58A3.2a.2; WBGene00001184. [Q10656-1]
F58A3.2a.3; F58A3.2a.3; WBGene00001184. [Q10656-1]
F58A3.2a.4; F58A3.2a.4; WBGene00001184. [Q10656-1]
GeneID181291.
KEGGcel:CELE_F58A3.2.
UCSCF58A3.2e. c. elegans. [Q10656-1]

Organism-specific databases

CTD181291.
WormBaseF58A3.2a; CE28238; WBGene00001184; egl-15.
F58A3.2b; CE35726; WBGene00001184; egl-15.
F58A3.2c; CE41325; WBGene00001184; egl-15.
F58A3.2d; CE31549; WBGene00001184; egl-15.
F58A3.2e; CE35727; WBGene00001184; egl-15.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00670000097694.
InParanoidQ10656.
OMARAHRPKE.
PhylomeDBQ10656.

Enzyme and pathway databases

BRENDA2.7.10.1. 1045.
SignaLinkQ10656.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF07679. I-set. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SMARTSM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50835. IG_LIKE. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio913304.

Entry information

Entry nameEGL15_CAEEL
AccessionPrimary (citable) accession number: Q10656
Secondary accession number(s): Q7JL68 expand/collapse secondary AC list , Q7YZM7, Q7YZM8, Q7YZN0, Q7YZN1, Q7Z025, Q8MQ14, Q93804, Q95QE0, Q95QE1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase