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Protein

Transcription factor elt-2

Gene

elt-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3' (PubMed:7782329, PubMed:15733671, PubMed:18003741, PubMed:18024960, PubMed:18448117, PubMed:26700680, PubMed:26016853). Predominantly directs the transcription of intestinal genes such as ges-1, cpr-6, pho-1, ftn-1 and lev-11, and itself (PubMed:9659934, PubMed:10518545, PubMed:15733671, PubMed:18448117, PubMed:19111532, PubMed:26700680). Required for gut-specific differentiation, specifically acting with the GATA region-binding transcription factor elt-7 to control normal gene expression and promote normal formation of the intestine (PubMed:20807527). Regulates intestinal gene expression in response to hypoxia to promote longevity (PubMed:25284791). Regulates tissue specific gene expression at basal levels and in response to bacterial infection in the intestine to control innate immununity (PubMed:16968778, PubMed:21168435, PubMed:25340560, PubMed:26016853). Plays a role in the induction of metal-responsive genes, activating gene expression from zinc-activated promoters and iron-dependent promoters and enhancers (PubMed:18024960, PubMed:22194696, PubMed:25552416). May regulate the expression of genes that control sensitivity to oxidative stress, in a mab-3-dependent manner, and osmotic stress, in conjunction with the GATA region-binding transcription factor elt-3 (PubMed:17901115, PubMed:20498281, PubMed:20126308). May play a role in sphingolipid signaling by regulating the expression of the sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735). May act with the Notch signaling pathway to promote endodermal gene expression (PubMed:18003741). Has a protective role in response to infection by Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen C.neoformans (PubMed:16968778, PubMed:17183709, PubMed:21168435, PubMed:23980181). May regulate gene expression during the recovery phase following a bacterial infection (PubMed:25340560). May act with p38-activated transcription factors to control p38 gene induction in response to bacterial infection (PubMed:22967128, PubMed:26016853). Controls lysosome formation in the intestine by controlling lysosomal gene expression (PubMed:26828939).23 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri237 – 261GATA-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: GO_Central
  • animal organ morphogenesis Source: GO_Central
  • cell development Source: GO_Central
  • cell fate commitment Source: GO_Central
  • defense response to Gram-negative bacterium Source: WormBase
  • digestion Source: WormBase
  • digestive tract development Source: GO_Central
  • endoderm development Source: WormBase
  • heart development Source: GO_Central
  • innate immune response Source: WormBase
  • nematode larval development Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter involved in defense response to Gram-negative bacterium Source: WormBase
  • regulation of epithelial cell differentiation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor elt-2Curated
Gene namesi
Name:elt-2Imported
ORF Names:C33D3.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC33D3.1; CE31430; WBGene00001250; elt-2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: WormBase
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Animals do not survive for long beyond hatching and frequently die at the L1 larval stage (PubMed:9659934, PubMed:20807527). Upon attempts to feed, food accumulates behind the pharynx and there is blockage of the brush border that surrounds the gut lumen in surviving L1 stage larvae (PubMed:9659934, PubMed:20807527). RNAi-mediated knockdown results in a reduced body size and reduced gene expression from zinc-activated promoters and of genes related to the immune response (PubMed:25552416, PubMed:26016853). This reduction in target gene expression is enhanced when infected with B. pseudomallei (PubMed:23980181). RNAi-mediated knockdown results in increased sensitivity and mortality when exposed to Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen C.neoformans (PubMed:16968778, PubMed:17183709, PubMed:21168435, PubMed:23980181). Furthermore, when exposed to E.coli and P.aeruginosa, the intestines distend due to the colonization and proliferation of its bacterial content (PubMed:16968778, PubMed:17183709, PubMed:21168435). RNAi-mediated knockdown also results in increased sensitivity to heat and to arsenite- and paraquat-induced oxidative stress (PubMed:16968778, PubMed:20498281). RNAi-mediated knockdown results in fewer intestinal lysosomes (PubMed:26828939). RNAi-mediated knockdown during larval development results in smaller animals (PubMed:26016853). RNAi-mediated knockdown specifically during the L4 larval stage inhibits the expression of intestinal genes such as gsto-1, which is normally induced under hypoxic conditions, prevents increased longevity induced by transient hypoxia exposure and prevents mitohormesis when exposed to the mitochondrial reactive oxygen species-generating agent paraquat (PubMed:25284791). In addition, RNAi-mediated knockdown at this stage prevents the expression of genes that are usually up-regulated during recovery in response to tetracycline or kanamycin treatment following an infection and furthermore, prevents the recovery of S. enterica infected animals treated with tetracycline (PubMed:25340560). RNAi-mediated knockdown on an elt-3 knockout background results in increased sensitivity to osmotic stress (PubMed:20126308). Double mutation with elt-7 results in arrest at the L1 stage of larval development, reduced expression of gut-specific genes and a severe disruption to normal gut differentiation with the absence of birefringent and rhabditin granules, which are characteristic of normal gut differentiation, largely at the regions of the cell that interface with the pharyngeal and rectal valves (PubMed:20807527). These mutants also have essentially no gut lumen, with the infrequent occurance of patches of lumen and brush border in few animals, and reduced gut epithelialization as indicated by reduced expression of epithelial markers erm-1b, itx-1 and ajm-1 (PubMed:20807527).13 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834721 – 433Transcription factor elt-2CuratedAdd BLAST433

Post-translational modificationi

May be ubiquitinated in response to infection by B.pseudomallei.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ10655.
PeptideAtlasiQ10655.

PTM databases

iPTMnetiQ10655.

Expressioni

Tissue specificityi

Expressed in the intestine.2 Publications

Developmental stagei

Expressed at all stages but more highly during embryogenesis, with expression beginning at the 2E cell stage (endodermal stage). Expression continues to adulthood.8 Publications

Inductioni

By P.aeruginosa infection.1 Publication

Gene expression databases

BgeeiWBGene00001250.

Interactioni

Subunit structurei

Interacts with lag-1 (PubMed:18003741). Interacts with pha-4 (PubMed:18448117).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ10655. 2 interactors.
STRINGi6239.C33D3.1.

Structurei

3D structure databases

ProteinModelPortaliQ10655.
SMRiQ10655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri237 – 261GATA-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000021020.
InParanoidiQ10655.
OMAiNIQVHVM.
OrthoDBiEOG091G0F8D.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNYNDNVN GWAEMEPSQP MGGLRLPTQN MDPPEQNNES QLSELPRMKI
60 70 80 90 100
DNDYASPIER QSVITSGTNN YEPKVETVTS FFHTGIDYSN FGMLDQTTMQ
110 120 130 140 150
PFYPLYSGIP VNTLGTFSGY TNSIYDKPSL YDPSIPTINI PSTYPTVAPT
160 170 180 190 200
YECVKCSQSC GAGMKAVNGG MMCVNCSTPK TTYSPPVAYS TSLGQPPILE
210 220 230 240 250
IPSEQPTAKI AKQSSKKSSS SNRGSNGSAS RRQGLVCSNC NGTNTTLWRR
260 270 280 290 300
NAEGDPVCNA CGLYFKLHHI PRPTSMKKEG ALQTRKRKSK SGDSSTPSTS
310 320 330 340 350
RARERKFERA SSSTEKAQRS SNRRAGSAKA DRELSTAAVA AATATYVSHA
360 370 380 390 400
DLYPVSSAAV TLPDQTYSNY YQWNTAATAG LMMVPNDQNY VYAATNYQTG
410 420 430
LRPADNIQVH VMPVQDDETK AAARDLEAVD GDS
Length:433
Mass (Da):47,116
Last modified:October 1, 1996 - v1
Checksum:i59C2DEB0753E5499
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25175 Genomic DNA. Translation: AAC36130.1.
Z49867 Genomic DNA. Translation: CAA90029.2.
PIRiA56953.
T19677.
RefSeqiNP_509755.2. NM_077354.4.
UniGeneiCel.6404.

Genome annotation databases

EnsemblMetazoaiC33D3.1; C33D3.1; WBGene00001250.
GeneIDi181250.
KEGGicel:CELE_C33D3.1.
UCSCiC33D3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25175 Genomic DNA. Translation: AAC36130.1.
Z49867 Genomic DNA. Translation: CAA90029.2.
PIRiA56953.
T19677.
RefSeqiNP_509755.2. NM_077354.4.
UniGeneiCel.6404.

3D structure databases

ProteinModelPortaliQ10655.
SMRiQ10655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ10655. 2 interactors.
STRINGi6239.C33D3.1.

PTM databases

iPTMnetiQ10655.

Proteomic databases

PaxDbiQ10655.
PeptideAtlasiQ10655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC33D3.1; C33D3.1; WBGene00001250.
GeneIDi181250.
KEGGicel:CELE_C33D3.1.
UCSCiC33D3.1. c. elegans.

Organism-specific databases

CTDi181250.
WormBaseiC33D3.1; CE31430; WBGene00001250; elt-2.

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000021020.
InParanoidiQ10655.
OMAiNIQVHVM.
OrthoDBiEOG091G0F8D.

Miscellaneous databases

PROiQ10655.

Gene expression databases

BgeeiWBGene00001250.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELT2_CAEEL
AccessioniPrimary (citable) accession number: Q10655
Secondary accession number(s): Q18371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.