Reviewed, UniProtKB/Swiss-Prot Q10651 (A4_CAEEL)

Names and origin

Protein names

Recommended name:
    Beta-amyloid-like protein

Gene names

Name:	apl-1
ORF Names:	C42D8.8

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the APP family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Amyloid Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase nervous system development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of locomotion Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q10651-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q10651-2)

The sequence of this isoform differs from the canonical sequence as follows:
     538-539: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 686	665	Beta-amyloid-like protein		PRO_0000000201
Regions
	Topological domain	22 – 621	600	Extracellular Potential
	Transmembrane	622 – 642	21	Potential
	Topological domain	643 – 686	44	Cytoplasmic Potential
	Motif	676 – 679	4	Clathrin-binding Potential
	Compositional bias	205 – 228	24	Asp-rich
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	538 – 539	2	Missing in isoform b.		VSP_000017

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified May 2, 2002. Version 2. Checksum: A0816858FDD48608	FASTA	686	79,435
	10 20 30 40 50 60 MTVGKLMIGL LIPILVATVY AEGSPAGSKR HEKFIPMVAF SCGYRNQYMT EEGSWKTDDE 70 80 90 100 110 120 RYATCFSGKL DILKYCRKAY PSMNITNIVE YSHEVSISDW CREEGSPCKW THSVRPYHCI 130 140 150 160 170 180 DGEFHSEALQ VPHDCQFSHV NSRDQCNDYQ HWKDEAGKQC KTKKSKGNKD MIVRSFAVLE 190 200 210 220 230 240 PCALDMFTGV EFVCCPNDQT NKTDVQKTKE DEDDDDDEDD AYEDDYSEES DEKDEEEPSS 250 260 270 280 290 300 QDPYFKIANW TNEHDDFKKA EMRMDEKHRK KVDKVMKEWG DLETRYNEQK AKDPKGAEKF 310 320 330 340 350 360 KSQMNARFQK TVSSLEEEHK RMRKEIEAVH EERVQAMLNE KKRDATHDYR QALATHVNKP 370 380 390 400 410 420 NKHSVLQSLK AYIRAEEKDR MHTLNRYRHL LKADSKEAAA YKPTVIHRLR YIDLRINGTL 430 440 450 460 470 480 AMLRDFPDLE KYVRPIAVTY WKDYRDEVSP DISVEDSELT PIIHDDEFSK NAKLDVKAPT 490 500 510 520 530 540 TTAKPVKETD NAKVLPTEAS DSEEEADEYY EDEDDEQVKK TPDMKKKVKV VDIKPKEIKV 550 560 570 580 590 600 TIEEEKKAPK LVETSVQTDD EDDDEDSSSS TSSESDEDED KNIKELRVDI EPIIDEPASF 610 620 630 640 650 660 YRHDKLIQSP EVERSASSVF QPYVLASAMF ITAICIIAFA ITNARRRRAM RGFIEVDVYT 670 680 PEERHVAGMQ VNGYENPTYS FFDSKA
	Isoform b. Checksum: 2E86C4ABD2CD0C59	FASTA	684	79,193
	10 20 30 40 50 60 MTVGKLMIGL LIPILVATVY AEGSPAGSKR HEKFIPMVAF SCGYRNQYMT EEGSWKTDDE 70 80 90 100 110 120 RYATCFSGKL DILKYCRKAY PSMNITNIVE YSHEVSISDW CREEGSPCKW THSVRPYHCI 130 140 150 160 170 180 DGEFHSEALQ VPHDCQFSHV NSRDQCNDYQ HWKDEAGKQC KTKKSKGNKD MIVRSFAVLE 190 200 210 220 230 240 PCALDMFTGV EFVCCPNDQT NKTDVQKTKE DEDDDDDEDD AYEDDYSEES DEKDEEEPSS 250 260 270 280 290 300 QDPYFKIANW TNEHDDFKKA EMRMDEKHRK KVDKVMKEWG DLETRYNEQK AKDPKGAEKF 310 320 330 340 350 360 KSQMNARFQK TVSSLEEEHK RMRKEIEAVH EERVQAMLNE KKRDATHDYR QALATHVNKP 370 380 390 400 410 420 NKHSVLQSLK AYIRAEEKDR MHTLNRYRHL LKADSKEAAA YKPTVIHRLR YIDLRINGTL 430 440 450 460 470 480 AMLRDFPDLE KYVRPIAVTY WKDYRDEVSP DISVEDSELT PIIHDDEFSK NAKLDVKAPT 490 500 510 520 530 540 TTAKPVKETD NAKVLPTEAS DSEEEADEYY EDEDDEQVKK TPDMKKKVKV VDIKPKEVTI 550 560 570 580 590 600 EEEKKAPKLV ETSVQTDDED DDEDSSSSTS SESDEDEDKN IKELRVDIEP IIDEPASFYR 610 620 630 640 650 660 HDKLIQSPEV ERSASSVFQP YVLASAMFIT AICIIAFAIT NARRRRAMRG FIEVDVYTPE 670 680 ERHVAGMQVN GYENPTYSFF DSKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"apl-1, a Caenorhabditis elegans gene encoding a protein related to the human beta-amyloid protein precursor." Daigle I., Li C. Proc. Natl. Acad. Sci. U.S.A. 90:12045-12049(1993) [PubMed: 8265668] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-686. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-249, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U56966 Genomic DNA. Translation: AAA98722.1. U56966 Genomic DNA. Translation: AAK68242.1. U00240 mRNA. Translation: AAC46470.1. Different initiation.
PIR	T15795.
RefSeq	NP_508870.3. NP_508871.1.
UniGene	Cel.6164
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25431N.
IntAct	Q10651. 1 interaction.
Genome annotation databases
Ensembl	C42D8.8a; C42D8.8a; C42D8.8; Caenorhabditis elegans. [Genome view]
GeneID	180783.
KEGG	cel:C42D8.8.
UCSC	C42D8.8a. c. elegans.
Organism-specific databases
CTD	180783.
WormBase	WBGene00000149. apl-1.
WormPep	C42D8.8a. CE04209. [WorfDB] C42D8.8b. CE27845. [WorfDB]
Phylogenomic databases
HOGENOM	HBG388116.
InParanoid	Q10651.
OMA	EYSHEVS.
Gene expression databases
ArrayExpress	Q10651.
Family and domain databases
InterPro	IPR008155. Amyloid_glyco. IPR011178. Amyloid_glyco_Cu-bd. IPR008154. Amyloid_glyco_extra. IPR019744. Amyloid_glyco_extracell_CS. IPR015849. Amyloid_glyco_heparin-bd. IPR019543. APP_amyloid. [Graphical view]
Gene3D	G3DSA:3.30.1490.140. Amyloid_glyco_Cu-bd. 1 hit. G3DSA:3.90.570.10. Amyloid_glyco_heparin-bd. 1 hit.
Pfam	PF02177. A4_EXTRA. 1 hit. PF10515. APP_amyloid. 1 hit. [Graphical view]
PRINTS	PR00203. AMYLOIDA4.
SMART	SM00006. A4_EXTRA. 1 hit. [Graphical view]
PROSITE	PS00319. A4_EXTRA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	910936.

Entry information

Entry name

A4_CAEEL

Accession

Primary (citable) accession number: Q10651
Secondary accession number(s): Q18583, Q95ZX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	December 15, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project