Q10639 (PHSG_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogen phosphorylase
EC=2.4.1.1

Gene names

Name:	glgP
Ordered Locus Names:	Rv1328, MT1370
ORF Names:	MTCY130.13

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties By similarity.
Catalytic activity	(1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.
Cofactor	Pyridoxal phosphate By similarity.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the glycogen phosphorylase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	Pyridoxal phosphate
Molecular function	Glycosyltransferase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	phosphorylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 863	863	Glycogen phosphorylase		PRO_0000188556
Amino acid modifications
	Modified residue	618	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	255	1	K → E in AAK45634. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q10639 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E39826B03D5B374F	FASTA	863	95,515
	10 20 30 40 50 60 MKALRRFTVR AHLPERLAAL DQLSTNLRWS WDKPTQDLFA AIDPALWEQC GHDPVALLGA 70 80 90 100 110 120 VNPARLDELA LDAEFLGALD ELAADLNDYL SRPLWYQEQQ DAGVAAQALP TGIAYFSLEF 130 140 150 160 170 180 GVAEVLPNYS GGLGILAGDH LKSASDLGVP LIAVGLYYRS GYFRQSLTAD GWQHETYPSL 190 200 210 220 230 240 DPQGLPLRLL TDANGDPVLV EVALGDNAVL RARIWVAQVG RVPLLLLDSD IPENEHDLRN 250 260 270 280 290 300 VTDRLYGGDQ EHRIKQEILA GIGGVRAIRA YTAVEKLTPP EVFHMNEGHA GFLGIERIRE 310 320 330 340 350 360 LVTDAGLDFD TALTVVRSST VFTTHTPVPA GIDRFPLEMV QRYVNDQRGD GRSRLLPGLP 370 380 390 400 410 420 ADRIVALGAE DDPAKFNMAH MGLRLAQRAN GVSLLHGRVS RAMFNELWAG FDPDEVPIGS 430 440 450 460 470 480 VTNGVHAPTW AAPQWLQLGR ELAGSDSLRE PVVWQRLHQV DPAHLWWIRS QLRSMLVEDV 490 500 510 520 530 540 RARLRQSWLE RGATDAELGW IATAFDPNVL TVGFARRVPT YKRLTLMLRD PDRLEQLLLD 550 560 570 580 590 600 EQRPIQLIVA GKSHPADDGG KALIQQVVRF ADRPQVRHRI AFLPNYDMSM ARLLYWGCDV 610 620 630 640 650 660 WLNNPLRPLE ACGTSGMKSA LNGGLNLSIR DGWWDEWYDG ENGWEIPSAD GVADENRRDD 670 680 690 700 710 720 LEAGALYDLL AQAVAPKFYE RDERGVPQRW VEMVRHTLQT LGPKVLASRM VRDYVEHYYA 730 740 750 760 770 780 PAAQSFRRTA GAQFDAAREL ADYRRRAEEA WPKIEIADVD STGLPDTPLL GSQLTLTATV 790 800 810 820 830 840 RLAGLRPNDV TVQGVLGRVD AGDVLMDPVT VEMAHTGTGD GGYEIFSTTT PLPLAGPVGY 850 860 TVRVLPRHPM LAASNELGLV TLA

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842576 Genomic DNA. Translation: CAA98092.1. AE000516 Genomic DNA. Translation: AAK45634.1.
PIR	D70770.
RefSeq	NP_215844.1. NC_000962.2. NP_335820.1. NC_002755.2.
3D structure databases
ProteinModelPortal	Q10639.
ModBase	Search...
Protein family/group databases
CAZy	GT35. Glycosyltransferase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003173; EBMYCP00000003173; EBMYCG00000003171. EBMYCT00000072654; EBMYCP00000070713; EBMYCG00000072649.
GeneID	886886. 924691.
GenomeReviews	Gene locus MT1370 in contig AE000516_GR. Gene locus Rv1328 in contig AL123456_GR.
KEGG	mtc:MT1370. mtu:Rv1328.
PATRIC	18124786. VBIMycTub22151_1508.
TIGR	MT1370.
Organism-specific databases
TubercuList	Rv1328.
Phylogenomic databases
GeneTree	EBGT00050000016289.
HOGENOM	HBG302557.
OMA	LRWSWHP.
PhylomeDB	Q10639.
ProtClustDB	CLSK871925.
Family and domain databases
InterPro	IPR011834. Agluc_phsphrylas. IPR000811. Glyco_trans_35. IPR024517. Glycogen_phosphorylase_DUF3417. [Graphical view]
KO	K00688.
PANTHER	PTHR11468. Glyco_trans_35. 1 hit.
Pfam	PF11897. DUF3417. 1 hit. PF00343. Phosphorylase. 1 hit. [Graphical view]
PIRSF	PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMs	TIGR02094. More_P_ylases. 1 hit.
PROSITE	PS00102. PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHSG_MYCTU

Accession

Primary (citable) accession number: Q10639

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program