Reviewed,
UniProtKB/Swiss-Prot Q10630 (ADA_MYCTU)
Last modified
June 16, 2009.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Putative regulatory protein ada Alternative name(s): Regulatory protein of adaptative response Including the following 1 domains: 1- Recommended name: Methylated-DNA--protein-cysteine methyltransferase EC=2.1.1.63 Alternative name(s): O-6-methylguanine-DNA alkyltransferase | ||||||||
| Gene names |
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| Organism | Mycobacterium tuberculosis [Complete proteome] [HAMAP] | ||||||||
| Taxonomic identifier | 1773 [NCBI] | ||||||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex |
Protein attributes
| Sequence length | 496 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine By similarity. The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus By similarity. |
| Catalytic activity | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Sequence similarities | In the C-terminal section; belongs to the MGMT family. Contains 1 HTH araC/xylS-type DNA-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair Transcription Transcription regulation |
| Ligand | DNA-binding Metal-binding Zinc |
| Molecular function | Activator Methyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | base-excision repair Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | intracellular Inferred from electronic annotation. Source: InterPro |
| Molecular function | methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC sequence-specific DNA bindingInferred from electronic annotation. Source: InterPro transcription factor activityInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 496 | 496 | Putative regulatory protein ada | PRO_0000139389 | |||||
Regions | |||||||||
| DNA binding | 103 – 122 | 20 | H-T-H motif By similarity | ||||||
| Compositional bias | 181 – 197 | 17 | Ala-rich | ||||||
Sites | |||||||||
| Active site | 34 | 1 | Nucleophile; methyl group acceptor from phosphotriester By similarity | ||||||
| Active site | 403 | 1 | Nucleophile; methyl group acceptor By similarity | ||||||
| Metal binding | 34 | 1 | Zinc By similarity | ||||||
| Metal binding | 38 | 1 | Zinc By similarity | ||||||
| Metal binding | 65 | 1 | Zinc By similarity | ||||||
| Metal binding | 68 | 1 | Zinc By similarity | ||||||
| Binding site | 30 | 1 | DNA By similarity | ||||||
| Binding site | 41 | 1 | DNA By similarity | ||||||
| Binding site | 63 | 1 | DNA By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| BX842576 Genomic DNA. Translation: CAA98104.1. U65786 Genomic DNA. Translation: AAB06751.1. AE000516 Genomic DNA. Translation: AAK45620.1. | |
| PIR | A70769. |
| RefSeq | NP_215833.1. NP_335806.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ADN based on UniProtKB P06134. |
| ModBase | Search... |
Protein family/group databases | |
| AraC-XylS | Search... |
Genome annotation databases | |
| GeneID | 886916. 924701. |
| GenomeReviews | Gene locus MT1358 in contig AE000516_GR. Gene locus Rv1317c in contig AL123456_GR. |
| KEGG | mtc:MT1358. mtu:Rv1317c. |
| TIGR | MT1358. |
Organism-specific databases | |
| TubercuList | Rv1317c. |
Phylogenomic databases | |
| HOGENOM | Q10630. |
| OMA | Q10630. RCRPDSA. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.63. 809. |
Family and domain databases | |
| InterPro | IPR004026. Ada_DNA_repair_Zn-bd. IPR010316. AlkA_N. IPR003583. Helix-hairpin-helix_DNA-bd. IPR003265. HhH-GPD_domain. IPR012287. Homeodomain-rel. IPR000005. HTH_AraC-typ. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_domain. [Graphical view] |
| Gene3D | G3DSA:3.40.10.10. Ada_DNA_repair_Zn-bd. 1 hit. G3DSA:3.30.310.20. AlkA_N. 1 hit. G3DSA:1.10.10.60. Homeodomain-rel. 1 hit. |
| Pfam | PF02805. Ada_Zn_binding. 1 hit. PF06029. AlkA_N. 1 hit. PF00730. HhH-GPD. 1 hit. PF00165. HTH_AraC. 2 hits. [Graphical view] |
| SMART | SM00478. ENDO3c. 1 hit. SM00278. HhH1. 1 hit. SM00342. HTH_ARAC. 1 hit. [Graphical view] |
| PROSITE | PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ADA_MYCTU | ||||||||
| Accession | Primary (citable) accession number: Q10630 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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