Reviewed, UniProtKB/Swiss-Prot Q10630 (ADA_MYCTU)

Names and origin

Protein names

Recommended name:
    Putative regulatory protein ada
Alternative name(s):
    Regulatory protein of adaptative response
Including the following 1 domains:
    1- Recommended name:
            Methylated-DNA--protein-cysteine methyltransferase
              EC=2.1.1.63
        Alternative name(s):
            O-6-methylguanine-DNA alkyltransferase

Gene names

Name:	ada
Synonyms:	alkA
Ordered Locus Names:	Rv1317c, MT1358
ORF Names:	MTCY130.02c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine By similarity. The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus By similarity.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	In the C-terminal section; belongs to the MGMT family. Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Putative regulatory protein ada		PRO_0000139389
Regions
	DNA binding	103 – 122	20	H-T-H motif By similarity
	Compositional bias	181 – 197	17	Ala-rich
Sites
	Active site	34	1	Nucleophile; methyl group acceptor from phosphotriester By similarity
	Active site	403	1	Nucleophile; methyl group acceptor By similarity
	Metal binding	34	1	Zinc By similarity
	Metal binding	38	1	Zinc By similarity
	Metal binding	65	1	Zinc By similarity
	Metal binding	68	1	Zinc By similarity
	Binding site	30	1	DNA By similarity
	Binding site	41	1	DNA By similarity
	Binding site	63	1	DNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10630-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CA1F52032181268F	FASTA	496	53,743
	10 20 30 40 50 60 MHDDFERCYR AIQSKDARFD GWFVVAVLTT GVYCRPSCPV RPPFARNVRF LPTAAAAQGE 70 80 90 100 110 120 GFRACKRCRP DASPGSPEWN VRSDVVARAM RLIADGTVDR DGVSGLAAQL GYTIRQLERL 130 140 150 160 170 180 LQAVVGAGPL ALARAQRMQT ARVLIETTNL PFGDVAFAAG FSSIRQFNDT VRLACDGTPT 190 200 210 220 230 240 ALRARAAARF ESATASAGTV SLRLPVRAPF AFEGVFGHLA ATAVPGCEEV RDGAYRRTLR 250 260 270 280 290 300 LPWGNGIVSL TPAPDHVRCL LVLDDFRDLM TATARCRRLL DLDADPEAIV EALGADPDLR 310 320 330 340 350 360 AVVGKAPGQR IPRTVDEAEF AVRAVLAQQV STKAASTHAG RLVAAYGRPV HDRHGALTHT 370 380 390 400 410 420 FPSIEQLAEI DPGHLAVPKA RQRTINALVA SLADKSLVLD AGCDWQRARG QLLALPGVGP 430 440 450 460 470 480 WTAEVIAMRG LGDPDAFPAS DLGLRLAAKK LGLPAQRRAL TVHSARWRPW RSYATQHLWT 490 TLEHPVNQWP PQEKIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Mycobacterium tuberculosis 3-methyladenine glycosidase and O6-methylguanine methyltransferase genes." Bourn W.R., Harington A., Wiid I., van Helden P.D. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 263-496. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842576 Genomic DNA. Translation: CAA98104.1. U65786 Genomic DNA. Translation: AAB06751.1. AE000516 Genomic DNA. Translation: AAK45620.1.
PIR	A70769.
RefSeq	NP_215833.1. NP_335806.1.
3D structure databases
HSSP	HSSP built from PDB template 1ADN based on UniProtKB P06134.
ModBase	Search...
Protein family/group databases
AraC-XylS	Search...
Genome annotation databases
GeneID	886916. 924701.
GenomeReviews	Gene locus MT1358 in contig AE000516_GR. Gene locus Rv1317c in contig AL123456_GR.
KEGG	mtc:MT1358. mtu:Rv1317c.
TIGR	MT1358.
Organism-specific databases
TubercuList	Rv1317c.
Phylogenomic databases
HOGENOM	Q10630.
OMA	Q10630. RCRPDSA.
Enzyme and pathway databases
BRENDA	2.1.1.63. 809.
Family and domain databases
InterPro	IPR004026. Ada_DNA_repair_Zn-bd. IPR010316. AlkA_N. IPR003583. Helix-hairpin-helix_DNA-bd. IPR003265. HhH-GPD_domain. IPR012287. Homeodomain-rel. IPR000005. HTH_AraC-typ. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_domain. [Graphical view]
Gene3D	G3DSA:3.40.10.10. Ada_DNA_repair_Zn-bd. 1 hit. G3DSA:3.30.310.20. AlkA_N. 1 hit. G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF02805. Ada_Zn_binding. 1 hit. PF06029. AlkA_N. 1 hit. PF00730. HhH-GPD. 1 hit. PF00165. HTH_AraC. 2 hits. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. SM00278. HhH1. 1 hit. SM00342. HTH_ARAC. 1 hit. [Graphical view]
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADA_MYCTU

Accession

Primary (citable) accession number: Q10630

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)