Q10630 (ALKA_MYCTU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 99.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Probable bifunctional transcriptional activator/DNA repair enzyme AlkA Alternative name(s): Regulatory protein AlkA | ||||||||
| Gene names |
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| Organism | Mycobacterium tuberculosis | ||||||||
| Taxonomic identifier | 1773 [NCBI] | ||||||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex |
Protein attributes
| Sequence length | 496 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Is involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs the Sp diastereomer of DNA methylphosphotriester lesions by a direct and irreversible transfer of the methyl group to one of its own cysteine residues. Also catalyzes the hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions By similarity. The methylation of Alka by methylphosphotriesters in DNA leads to its activation as a transcriptional regulator that activates the transcription of its own gene and other alkylation resistance genes By similarity. |
| Catalytic activity | DNA (containing Sp-methylphosphotriester) + protein L-cysteine = DNA (without Sp-methylphosphotriester) + protein S-methyl-L-cysteine. Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Miscellaneous | This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles. |
| Sequence similarities | In the C-terminal section; belongs to the alkylbase DNA glycosidase AlkA family. Contains 1 HTH araC/xylS-type DNA-binding domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 496 | 496 | Probable bifunctional transcriptional activator/DNA repair enzyme AlkA | PRO_0000139389 | |||||
Regions | |||||||||
| Domain | 87 – 185 | 99 | HTH araC/xylS-type | ||||||
| DNA binding | 104 – 125 | 22 | H-T-H motif By similarity | ||||||
| Region | 1 – 185 | 185 | Methylphosphotriester-DNA--protein-cysteine methyltransferase | ||||||
| Region | 202 – 354 | 153 | DNA-3-methyladenine glycosylase | ||||||
| Compositional bias | 181 – 197 | 17 | Ala-rich | ||||||
Sites | |||||||||
| Active site | 34 | 1 | Nucleophile; methyl group acceptor from methylphosphotriester By similarity | ||||||
| Active site | 441 | 1 | Proton acceptor; for DNA glycosylase activity By similarity | ||||||
| Metal binding | 34 | 1 | Zinc By similarity | ||||||
| Metal binding | 38 | 1 | Zinc By similarity | ||||||
| Metal binding | 65 | 1 | Zinc By similarity | ||||||
| Metal binding | 68 | 1 | Zinc By similarity | ||||||
| Binding site | 30 | 1 | DNA By similarity | ||||||
| Binding site | 41 | 1 | DNA By similarity | ||||||
| Binding site | 63 | 1 | DNA By similarity | ||||||
| Site | 421 | 1 | Determinant for substrate specificity and/or DNA glycosylase activity By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BX842576 Genomic DNA. Translation: CAA98104.1. U65786 Genomic DNA. Translation: AAB06751.1. AE000516 Genomic DNA. Translation: AAK45620.1. |
| PIR | A70769. |
| RefSeq | NP_215833.1. NC_000962.2. NP_335806.1. NC_002755.2. |
3D structure databases | |
| ProteinModelPortal | Q10630. |
| ModBase | Search... |
Protein family/group databases | |
| AraC-XylS | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBMYCT00000003926; EBMYCP00000003926; EBMYCG00000003924. EBMYCT00000071713; EBMYCP00000069772; EBMYCG00000071708. |
| GeneID | 886916. 924701. |
| GenomeReviews | Gene locus MT1358 in contig AE000516_GR. Gene locus Rv1317c in contig AL123456_GR. |
| KEGG | mtc:MT1358. mtu:Rv1317c. |
| PATRIC | 18124756. VBIMycTub22151_1493. |
| TIGR | MT1358. |
Organism-specific databases | |
| TubercuList | Rv1317c. |
Phylogenomic databases | |
| GeneTree | EBGT00050000015628. |
| HOGENOM | HBG510250. |
| OMA | RCRPDSA. |
| PhylomeDB | Q10630. |
| ProtClustDB | CLSK791096. |
Family and domain databases | |
| InterPro | IPR004026. Ada_DNA_repair_Zn-bd. IPR010316. AlkA_N. IPR011257. DNA_glycosylase. IPR003265. HhH-GPD_domain. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_DNA-bd. IPR023170. HTH_base_excis_C. IPR012294. TFIID_C/glycos_N. [Graphical view] |
| Gene3D | G3DSA:3.40.10.10. Ada_DNA_repair_Zn-bd. 1 hit. G3DSA:3.30.310.20. AlkA_N. 1 hit. G3DSA:1.10.340.30. DNA_glycosylase. 1 hit. G3DSA:1.10.10.60. Homeodomain-rel. 1 hit. G3DSA:1.10.1670.10. HTH_base_excis_C. 1 hit. |
| KO | K13529. |
| Pfam | PF02805. Ada_Zn_binding. 1 hit. PF06029. AlkA_N. 1 hit. PF00730. HhH-GPD. 1 hit. [Graphical view] |
| SMART | SM01009. AlkA_N. 1 hit. SM00478. ENDO3c. 1 hit. SM00278. HhH1. 1 hit. SM00342. HTH_ARAC. 1 hit. [Graphical view] |
| SUPFAM | SSF57884. Ada_DNA_repair_Zn-bd. 1 hit. SSF48150. DNA_glycsylse. 1 hit. SSF46689. Homeodomain_like. 1 hit. SSF55945. TFIID_C/glycos_N. 1 hit. |
| PROSITE | PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ALKA_MYCTU | ||||||||
| Accession | Primary (citable) accession number: Q10630 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with